Cardoso, Isabel; Bartsch, Inka; Valentin, Klaus; Steinhagen, Sophie; Buck, Bela Hieronymus; Hofmann, Laurie C: Naturally occurring protoplasts in two Ulva spp. - Disc sizes from discs grown from protoplasts, after 35 days of germination, under culture conditions [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.972125 (dataset in review)
Abstract:
Degradation in Ulva lacinulata and reproduction in Ulva compressa were observed and described for four weeks. The natural occurrence of protoplasts in both species was found. Protoplast yields were calculated each week for several weeks. At the beginning of the experiment, heterogeneity of the thallus was visible under the microscope. The diameter of the cells in the thalli that later became protoplasts were measured. A germination experiment was performed after protoplasts were collected. Germination rates were calculated based on the number of individuals visible by the naked eye at the end of the experiment. 2 % (U. lacinulata) and 4 % (U. compressa) of protoplasts germinated into two morphologies: unattached germlings and unattached discs. Because discs released gametes early in their development, their diameter was measured to estimate the gametes yields produced at the end of the experiment. During degradation of U. lacinulata, we found an increase in biomass after the first two weeks of the experiment. Fresh weight measurements were taken each week and relative growth rates were calculated. Our results provide the first evidence of the natural production of protoplasts in Ulva spp. and estimate that higher seeding yields can be obtained by natural protoplast production than direct gametogenesis.
Keyword(s):
Related to:
Cardoso, Isabel; et al. (in review): Naturally occurring protoplasts in Ulva spp. reveal a previously underestimated proliferation process.
Funding:
Federal Ministry of Food and Agriculture (BMEL), grant/award no. 281DL02B20: Verbundprojekt: Industrietaugliche Verfahrensoptimierung zur Herstellung einer nachhaltigen Verpackungslösung aus Makroalgen für den Lebensmittel-Handel (MakPakScaleUp) - Teilprojekt B
Swedish Research Council (VR), grant/award no. 2022-00331_Formas: A manual for the use of sustainable marine resources – Definition of the cultivation potential and commercially relevant compounds of Sea Lettuces (Ulva spp.) along the Swedish coastline
Coverage:
Median Latitude: 45.358263 * Median Longitude: -1.844892 * South-bound Latitude: 39.394977 * West-bound Longitude: -9.213583 * North-bound Latitude: 53.741889 * East-bound Longitude: 8.514556
Date/Time Start: 2021-01-04T12:00:00 * Date/Time End: 2023-07-24T10:00:00
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Size:
5 datasets
Download Data (login required; moratorium until 2025-01-29)
Datasets listed in this bundled publication
- Cardoso, I; Bartsch, I; Valentin, K et al. (in review): Diameter of the discs grown from protoplasts of Ulva lacinulata at the end of the germination experiment. https://doi.pangaea.de/10.1594/PANGAEA.972127
- Cardoso, I; Bartsch, I; Valentin, K et al. (in review): Number of protoplasts collected during the degradation experiment with Ulva lacinulata and Ulva compressa. https://doi.pangaea.de/10.1594/PANGAEA.972132
- Cardoso, I; Bartsch, I; Valentin, K et al. (in review): Fresh weight of Ulva lacinulata's biomass during the degradation experiment. https://doi.pangaea.de/10.1594/PANGAEA.972133
- Cardoso, I; Bartsch, I; Valentin, K et al. (in review): Diameter of the larger green cells found in the thalli of Ulva lacinulata and Ulva compressa that became protoplasts after being released to the water. https://doi.pangaea.de/10.1594/PANGAEA.972134
- Cardoso, I; Bartsch, I; Valentin, K et al. (in review): Number of germinated individuals that originated from protoplasts from Ulva lacinulata and Ulva compressa. https://doi.pangaea.de/10.1594/PANGAEA.972135