Cardoso, Isabel; Bartsch, Inka; Valentin, Klaus; Steinhagen, Sophie; Buck, Bela Hieronymus; Hofmann, Laurie C: Diameter of the discs grown from protoplasts of Ulva lacinulata at the end of the germination experiment [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.972127 (dataset in review), In: Cardoso, I et al.: Naturally occurring protoplasts in two Ulva spp. - Disc sizes from discs grown from protoplasts, after 35 days of germination, under culture conditions [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.972125 (dataset in review)
Abstract:
This dataset presents the diameter of the discs grown from protoplasts of Ulva lacinulata at the end of the germination experiment.
Keyword(s):
Related to:
Cardoso, Isabel; et al. (in review): Naturally occurring protoplasts in Ulva spp. reveal a previously underestimated proliferation process.
References:
Provasoli, L (1968): Media and prospects for the cultivation of marine algae. In: Cultures and collections of algae. Proceedings of US-Japan
Conference. Hakone, 63–75
Tatewaki, Masakazu (1966): Formation of a Crustaceous Sporophyte with Unilocular Sporangia in Scytosiphon lomentaria. Phycologia, 6(1), 62-66, https://doi.org/10.2216/i0031-8884-6-1-62.1
Funding:
Federal Ministry of Food and Agriculture (BMEL), grant/award no. 281DL02B20: Verbundprojekt: Industrietaugliche Verfahrensoptimierung zur Herstellung einer nachhaltigen Verpackungslösung aus Makroalgen für den Lebensmittel-Handel (MakPakScaleUp) - Teilprojekt B
Swedish Research Council (VR), grant/award no. 2022-00331_Formas: A manual for the use of sustainable marine resources – Definition of the cultivation potential and commercially relevant compounds of Sea Lettuces (Ulva spp.) along the Swedish coastline
Coverage:
Latitude: 39.394977 * Longitude: -9.213583
Date/Time Start: 2021-01-04T12:00:00 * Date/Time End: 2021-01-04T12:00:00
Minimum DEPTH, water: 0 m * Maximum DEPTH, water: 0 m
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Hofmann, Laurie C | Original Sample collection | ||
| 2 | DATE/TIME | Date/Time | Hofmann, Laurie C | Geocode – Original Sample collection | ||
| 3 | LATITUDE | Latitude | Hofmann, Laurie C | Google Maps | Geocode – Original Sample collection | |
| 4 | LONGITUDE | Longitude | Hofmann, Laurie C | Google Maps | Geocode – Original Sample collection | |
| 5 | DEPTH, water | Depth water | m | Hofmann, Laurie C | Geocode – The species were collected by the principal investigator during low tide in mudflat areas were there was no water (at that specific time) | |
| 6 | Type of study | Study type | Hofmann, Laurie C | |||
| 7 | Laboratory | Lab | Hofmann, Laurie C | |||
| 8 | Latitude 2 | Latitude 2 | Hofmann, Laurie C | Google Maps | of laboratory | |
| 9 | Longitude 2 | Longitude 2 | Hofmann, Laurie C | Google Maps | of laboratory | |
| 10 | Date/time start, experiment | Date/time start exp | Hofmann, Laurie C | |||
| 11 | Date/time end, experiment | Date/time end exp | Hofmann, Laurie C | |||
| 12 | Day of experiment | DOE | day | Hofmann, Laurie C | time of measurement | |
| 13 | Species | Species | Hofmann, Laurie C | |||
| 14 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Hofmann, Laurie C | |||
| 15 | Species, unique identification (URI) | Species UID (URI) | Hofmann, Laurie C | |||
| 16 | Collection | Collection | Hofmann, Laurie C | Collection Number | ||
| 17 | Accession number, genetics | Access no gen | Steinhagen, Sophie | GenBank NCBI | Molecular analysis with the Invisorb Spin Plant Mini Kit (Stratec); gel electrophoresis and subsequently purified using the QIAquick PCR Purification Kit (Qiagen); Sanger sequencing was performed by Eurofins Genomics (Konstanz, Germany); Sequence reads were assembled in sequencher (version 4.1.4, Gene Codes Corporation). BLAST function in GenBank, identifications via the specimens' tufA sequences were made. | |
| 18 | Replicate | Repl | Hofmann, Laurie C | |||
| 19 | Treatment: temperature | T:temp | °C | Hofmann, Laurie C | Digital thermometer | |
| 20 | Treatment: salinity | T:sal | Hofmann, Laurie C | Refractometer | PSU | |
| 21 | Treatment: light condition | T:light | Hofmann, Laurie C | Day length: h, light:dark | ||
| 22 | Treatment: light intensity | T:Io | µmol/m2/s | Hofmann, Laurie C | Light meter, LI-COR Biosciences GmbH, LI-250A | |
| 23 | Treatment: nutrients | T:nutrients | Hofmann, Laurie C | Nutrient Source; Provasoli's enriched seawater (PES), half-strength (Provasoli 1986; Tatewaki 1966) | ||
| 24 | Treatment: nutrients | T:nutrients | Hofmann, Laurie C | Nutrient Source - Concentration [mL L-1] | ||
| 25 | Diameter | Ø | mm | Hofmann, Laurie C | ImageJ software | Diameter of Ulva individuals with an unnatached disc morphology |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
190 data points