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Cardoso, Isabel; Bartsch, Inka; Valentin, Klaus; Steinhagen, Sophie; Buck, Bela Hieronymus; Hofmann, Laurie C: Diameter of the larger green cells found in the thalli of Ulva lacinulata and Ulva compressa that became protoplasts after being released to the water [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.972134 (dataset in review), In: Cardoso, I et al.: Naturally occurring protoplasts in two Ulva spp. - Disc sizes from discs grown from protoplasts, after 35 days of germination, under culture conditions [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.972125 (dataset in review)

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Abstract:
This dataset presents the diameter of the larger green cells found in the thalli of Ulva lacinulata and Ulva compressa that became protoplasts after being released to the water.
Keyword(s):
degradation; Laboratory experiment; pincuschion; protoplasts; Reproduction; Ulva; Ulva compressa; Ulva lacinulata
Related to:
Cardoso, Isabel; et al. (in review): Naturally occurring protoplasts in Ulva spp. reveal a previously underestimated proliferation process.
References:
Provasoli, L (1968): Media and prospects for the cultivation of marine algae. In: Cultures and collections of algae. Proceedings of US-Japan Conference. Hakone, 63–75
Tatewaki, Masakazu (1966): Formation of a Crustaceous Sporophyte with Unilocular Sporangia in Scytosiphon lomentaria. Phycologia, 6(1), 62-66, https://doi.org/10.2216/i0031-8884-6-1-62.1
Funding:
Federal Ministry of Food and Agriculture (BMEL), grant/award no. 281DL02B20: Verbundprojekt: Industrietaugliche Verfahrensoptimierung zur Herstellung einer nachhaltigen Verpackungslösung aus Makroalgen für den Lebensmittel-Handel (MakPakScaleUp) - Teilprojekt B
Swedish Research Council (VR), grant/award no. 2022-00331_Formas: A manual for the use of sustainable marine resources – Definition of the cultivation potential and commercially relevant compounds of Sea Lettuces (Ulva spp.) along the Swedish coastline
Coverage:
Median Latitude: 50.689355 * Median Longitude: 4.742611 * South-bound Latitude: 39.394977 * West-bound Longitude: -9.213583 * North-bound Latitude: 53.741889 * East-bound Longitude: 8.514556
Date/Time Start: 2021-01-04T12:00:00 * Date/Time End: 2023-07-24T10:00:00
Minimum DEPTH, water: 0 m * Maximum DEPTH, water: 0 m
Event(s):
Ulva_Dorum * Latitude: 53.741889 * Longitude: 8.514556 * Date/Time: 2023-07-24T10:00:00 * Method/Device: Biological sample (BIOS) * Comment: Species were collected during low tide in a Lagoon were there was no water (at that specific time)
Ulva_NE-Atlantic * Latitude: 39.394977 * Longitude: -9.213583 * Date/Time: 2021-01-04T12:00:00 * Method/Device: Biological sample (BIOS) * Comment: Species were collected during low tide in a Lagoon were there was no water (at that specific time)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventHofmann, Laurie COriginal Sample collection
2DATE/TIMEDate/TimeHofmann, Laurie CGeocode – Original Sample collection
3LATITUDELatitudeHofmann, Laurie CGoogle MapsGeocode – Original Sample collection
4LONGITUDELongitudeHofmann, Laurie CGoogle MapsGeocode – Original Sample collection
5DEPTH, waterDepth watermHofmann, Laurie CGeocode – The species were collected by the principal investigator during low tide in mudflat areas were there was no water (at that specific time)
6Type of studyStudy typeHofmann, Laurie C
7LaboratoryLabHofmann, Laurie C
8Latitude 2Latitude 2Hofmann, Laurie CGoogle Mapsof laboratory
9Longitude 2Longitude 2Hofmann, Laurie CGoogle Mapsof laboratory
10Date/time start, experimentDate/time start expHofmann, Laurie C
11Date/time end, experimentDate/time end expHofmann, Laurie C
12Day of experimentDOEdayHofmann, Laurie Ctime of measurement
13SpeciesSpeciesHofmann, Laurie C
14Species, unique identification (Semantic URI)Species UID (Semantic URI)Hofmann, Laurie C
15Species, unique identification (URI)Species UID (URI)Hofmann, Laurie C
16CollectionCollectionHofmann, Laurie CCollection Number
17Accession number, geneticsAccess no genSteinhagen, SophieGenBank NCBIMolecular analysis with the Invisorb Spin Plant Mini Kit (Stratec); gel electrophoresis and subsequently purified using the QIAquick PCR Purification Kit (Qiagen); Sanger sequencing was performed by Eurofins Genomics (Konstanz, Germany); Sequence reads were assembled in sequencher (version 4.1.4, Gene Codes Corporation). BLAST function in GenBank, identifications via the specimens' tufA sequences were made.
18ReplicateReplHofmann, Laurie C
19ReplicateReplHofmann, Laurie CSubreplicate
20Treatment: temperatureT:temp°CHofmann, Laurie CDigital thermometer
21Treatment: salinityT:salHofmann, Laurie CRefractometerPSU
22Treatment: light conditionT:lightHofmann, Laurie CDay length: h, light:dark
23Treatment: light intensityT:Ioµmol/m2/sHofmann, Laurie CLight meter, LI-COR Biosciences GmbH, LI-250A
24Treatment: nutrientsT:nutrientsHofmann, Laurie CNutrient Source; Provasoli's enriched seawater (PES), half-strength (Provasoli 1986; Tatewaki 1966)
25Treatment: nutrientsT:nutrientsHofmann, Laurie CNutrient Source - Concentration [mL L-1]
26DiameterصmHofmann, Laurie CImageJ softwareDiameter of Ulva protoplasts
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1806 data points

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