Citation:

PANGAEA.971946 (dataset in review)

Name: Stable isotopes and mol% of amino acids in seston at surface during ENDEAVOR cruise EN614
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Amazon River plume; amino acids mol%; Compound-specific stable isotope analyses of amino acids; seston; stable carbon isotopes δ13C; stable nitrogen isotope (δ15N)

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Abstract:

The Amazon river accounts annually for 20% of riverine discharge into the global ocean with strong seasonal variations. This lens of freshwater spreads at surface as a plume, and can reach as far as the Caribbean. During this journey, the changing physicochemical characteristics of the aging plume define contrasting plankton habitats. These habitats select for communities with varying degrees of complexity of the plankton food web. During ENDEAVOR cruise EN614 (https://demo.bco-dmo.org/dataset/757784) we studied the dominant trophic function at the base of the food web (autotrophs vs mixotrophs) using stable isotopes of nitrogen in specific amino acids as well as bulk isotopes of carbon and nitrogen. This dataset contains the nitrogen signatures of 12 amino acids in δ notation (δ15N, ‰ vs N2 in air), the mol% of 13 amino acids and the carbon (δ13C, ‰ vs VPDB) and nitrogen isotopes in bulk samples at surface as well as environmental data and phytoplankton community structure.

Keyword(s):

Amazon River plume; amino acids mol%; Compound-specific stable isotope analyses of amino acids; seston; stable carbon isotopes δ13C; stable nitrogen isotope (δ15N)

Supplement to:

Fernández-Carrera, Ana; et al. (submitted): Mixotrophy emerges as the optimal strategy in mature waters of the Amazon River plume.

Related to:

Fernández-Carrera, Ana; Steinkopf, Markus; Liskow, Iris; Wodarg, Dirk; Voss, Maren; Loick-Wilde, Natalie: Stable isotopes and mol% of amino acids in 0.2-3 µm seston at surface during METEOR cruise M174 [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.971293

Fernández-Carrera, Ana; Steinkopf, Markus; Liskow, Iris; Wodarg, Dirk; Voss, Maren; Loick-Wilde, Natalie: Stable isotopes and mol% of amino acids in 3-200 µm seston at surface during METEOR cruise M174 [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.971279

References:

Bricaud, Annick; Claustre, Hervé; Ras, Joséphine; Oubelkheir, Kadija (2004): Natural variability of phytoplanktonic absorption in oceanic waters: Influence of the size structure of algal populations. Journal of Geophysical Research: Oceans, 109(C11), 2004JC002419, https://doi.org/10.1029/2004JC002419

Grasshoff, Klaus; Kremling, Klaus; Ehrhardt, M (1999): Methods of Seawater Analysis, Third Edition. Wiley-VCH, https://doi.org/10.1002/9783527613984.fmatter

Pham, Anh H; Choisnard, Noémie; Fernández-Carrera, Ana; Subramaniam, Ajit; Strope, Erica K; Carpenter, Edward J; Voss, Maren; Montoya, Joseph P (2024): Planktonic habitats in the Amazon Plume region of the Western Tropical North Atlantic. Frontiers in Marine Science, 11, 1287497, https://doi.org/10.3389/fmars.2024.1287497

Weber, Sarah C; Subramaniam, Ajit; Montoya, Joseph P; Doan-Nhu, Hai; Nguyen-Ngoc, Lam; Dippner, Joachim W; Voss, Maren (2019): Habitat Delineation in Highly Variable Marine Environments. Frontiers in Marine Science, 6, 112, https://doi.org/10.3389/fmars.2019.00112

Funding:

Deutsche Forschungsgemeinschaft, Bonn (DFG), grant/award no. 439440452 : Metabolismus des Stickstoffs in der Amazonasfahne und dem westlichen, tropischen Nordatlantik (MeNARP)

National Science Foundation (NSF), grant/award no. OCE-1737078: Impact of the Amazon River Plume on Nitrogen Availability and Planktonic Food Web Dynamics in the Western Tropical North Atlantic (Amazon River Plume Nitrogen)

Coverage:

Median Latitude: 10.275539 * Median Longitude: -54.715836 * South-bound Latitude: 4.889500 * West-bound Longitude: -57.252960 * North-bound Latitude: 16.289230 * East-bound Longitude: -50.892740

Date/Time Start: 2018-05-09T23:34:00 * Date/Time End: 2018-05-29T15:02:00

Minimum DEPTH, water: 2.8 m * Maximum DEPTH, water: 10.5 m

Event(s):

EN614_3-09 * Latitude: 8.575890 * Longitude: -54.518280 * Date/Time: 2018-05-09T23:34:00 * Location: western tropical Atlantic * Campaign: * Basis: Endeavor * Method/Device: (CTD-RO)

EN614_6-04 * Latitude: 7.330560 * Longitude: -55.597440 * Date/Time: 2018-05-11T21:15:00 * Location: western tropical Atlantic * Campaign: * Basis: Endeavor * Method/Device: (CTD-RO)

EN614_8-02 * Latitude: 7.365010 * Longitude: -53.818070 * Date/Time: 2018-05-12T16:26:00 * Location: western tropical Atlantic * Campaign: * Basis: Endeavor * Method/Device: (CTD-RO)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Loick-Wilde, Natalie
2	Station label	Station		Loick-Wilde, Natalie
3	Campaign	Campaign		Loick-Wilde, Natalie
4	DATE/TIME	Date/Time		Loick-Wilde, Natalie		Geocode
5	LATITUDE	Latitude		Loick-Wilde, Natalie		Geocode
6	LONGITUDE	Longitude		Loick-Wilde, Natalie		Geocode
7	Location	Location		Loick-Wilde, Natalie
8	Habitat	Habitat		Loick-Wilde, Natalie	Habitat Delineation in Highly Variable Marine Environments (Weber et. al, 2019)
9	DEPTH, water	Depth water	m	Loick-Wilde, Natalie	CTD/Rosette (CTD-RO)	Geocode
10	Salinity	Sal		Loick-Wilde, Natalie	CTD/Rosette (CTD-RO)	psu
11	Temperature, water	Temp	°C	Loick-Wilde, Natalie	CTD/Rosette (CTD-RO)
12	Oxygen	O2	µmol/l	Loick-Wilde, Natalie	CTD/Rosette (CTD-RO)
13	Silicate	Si(OH)4	µmol/l	Montoya, Joseph P	Methods of Seawater Analysis, Third Edition (Grasshoff et al., 1999)
14	Nitrite	[NO2]-	µmol/l	Montoya, Joseph P	Methods of Seawater Analysis, Third Edition (Grasshoff et al., 1999)
15	Phosphate	[PO4]3-	µmol/l	Montoya, Joseph P	Methods of Seawater Analysis, Third Edition (Grasshoff et al., 1999)
16	Nitrate	[NO3]-	µmol/l	Montoya, Joseph P	Methods of Seawater Analysis, Third Edition (Grasshoff et al., 1999)
17	Depth of nitracline	Depth nitracline	m	Loick-Wilde, Natalie	Linear interpolation in nutrient depth profile	1µM
18	Depth, phosphocline	PO4-cline	m	Loick-Wilde, Natalie	Linear interpolation in nutrient depth profile	0.1µM
19	Depth, silicocline	Si-cline	m	Loick-Wilde, Natalie	Linear interpolation in nutrient depth profile	2µM
20	Chlorophyll a	Chl a	µg/l	Loick-Wilde, Natalie	High performance liquid chromatography (HPLC), Agilent 1200
21	High-pressure liquid chromatography size index	HPLC Size index	µm	Loick-Wilde, Natalie	Based on HPLC pigments according to Bricaud et al (2004)
22	River plume thickness	River plume thickness	m	Loick-Wilde, Natalie	Linear interpolation depth of 35 PSU
23	Brunt-Väisälä frequency, maximum	N2 max	1/s²	Loick-Wilde, Natalie
24	Mixed layer depth	MLD	m	Loick-Wilde, Natalie
25	Depth, deep chlorophyll maximum	DCM depth	m	Loick-Wilde, Natalie
26	Filamentous Cyanobacteria	Filamentous cyanob	%	Loick-Wilde, Natalie	ChemTax v 1.9.5 (Wright, S., 2017)
27	Synechococcus	Synechococcus	%	Loick-Wilde, Natalie	ChemTax v 1.9.5 (Wright, S., 2017)
28	Prochlorococcus	Prochlorococcus	%	Loick-Wilde, Natalie	ChemTax v 1.9.5 (Wright, S., 2017)
29	Prasinophytes	Prasinophytes	%	Loick-Wilde, Natalie	ChemTax v 1.9.5 (Wright, S., 2017)
30	Cryptophytes	Cryptophytes	%	Loick-Wilde, Natalie	ChemTax v 1.9.5 (Wright, S., 2017)
31	Diatoms	Diatoms	%	Loick-Wilde, Natalie	ChemTax v 1.9.5 (Wright, S., 2017)
32	Haptophytes	Haptophytes	%	Loick-Wilde, Natalie	ChemTax v 1.9.5 (Wright, S., 2017)
33	Dinoflagellates	Dinofl	%	Loick-Wilde, Natalie	ChemTax v 1.9.5 (Wright, S., 2017)
34	Carbon, particulate	PC	µmol/l	Montoya, Joseph P	Continuous flow isotope ratio mass spectrometer (CF-IRMS), Micromass, Isoprime; coupled with Elemental analyser, Carlo Erba, NA 2500
35	δ13C, seston	δ13C sest	‰ PDB	Montoya, Joseph P	Continuous flow isotope ratio mass spectrometer (CF-IRMS), Micromass, Isoprime; coupled with Elemental analyser, Carlo Erba, NA 2500
36	Nitrogen, particulate	PN	µmol/l	Montoya, Joseph P	Continuous flow isotope ratio mass spectrometer (CF-IRMS), Micromass, Isoprime; coupled with Elemental analyser, Carlo Erba, NA 2500
37	δ15N, seston	δ15N sest	‰ air	Montoya, Joseph P	Continuous flow isotope ratio mass spectrometer (CF-IRMS), Micromass, Isoprime; coupled with Elemental analyser, Carlo Erba, NA 2500
38	Glutamine and Glutamic acid, δ15N	Gln + Glu δ15N	‰ air	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
39	Glutamine and Glutamic acid, δ15N standard deviation	Gln + Glu δ15N std dev	±	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
40	Phenylalanine, δ15N	Phe δ15N	‰ air	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
41	Phenylalanine, δ15N, standard deviation	Phe δ15N std dev	±	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
42	Alanine, δ15N	Ala δ15N	‰ air	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
43	Alanine, δ15N, standard deviation	Ala δ15N std dev	±	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
44	Valine, δ15N	Val δ15N	‰ air	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
45	Valine, δ15N, standard deviation	Val δ15N std dev	±	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
46	Leucine, δ15N	Leu δ15N	‰ air	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
47	Leucine, δ15N, standard deviation	Leu δ15N std dev	±	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
48	Isoleucine, δ15N	Ile δ15N	‰ air	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
49	Isoleucine, δ15N, standard deviation	Ile δ15N std dev	±	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
50	Proline, δ15N	Pro δ15N	‰ air	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
51	Proline, δ15N, standard deviation	Pro δ15N std dev	±	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
52	Aspartamine and Aspartic acid, δ15N	Asn + Asp δ15N	‰ air	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
53	Aspartamine and Aspartic acid, δ15N standard deviation	Asn + Asp δ15N std dev	±	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
54	Lysine, δ15N	Lys δ15N	‰ air	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
55	Lysine, δ15N, standard deviation	Lys δ15N std dev	±	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
56	Tyrosine, δ15N	Tyr δ15N	‰ air	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
57	Tyrosine, δ15N standard deviation	Tyr δ5N std dev	±	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
58	Serine, δ15N	Ser δ15N	‰ air	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
59	Serine, δ15N, standard deviation	Ser δ15N std dev	±	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
60	Threonine, δ15N	Thr δ15N	‰ air	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
61	Threonine, δ15N, standard deviation	Thr δ15N std dev	±	Loick-Wilde, Natalie	Isotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
62	Glutamine and Glutamic acid	Gln + Glu	%	Loick-Wilde, Natalie	Gas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finnigan	mol%
63	Phenylalanine	Phe	%	Loick-Wilde, Natalie	Gas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finnigan	mol%
64	Alanine	Ala	%	Loick-Wilde, Natalie	Gas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finnigan	mol%
65	Valine	Val	%	Loick-Wilde, Natalie	Gas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finnigan	mol%
66	Leucine	Leu	%	Loick-Wilde, Natalie	Gas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finnigan	mol%
67	Isoleucine	Ile	%	Loick-Wilde, Natalie	Gas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finnigan	mol%
68	Proline	Pro	%	Loick-Wilde, Natalie	Gas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finnigan	mol%
69	Aspartamine and Aspartic acid	Asn + Asp	%	Loick-Wilde, Natalie	Gas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finnigan	mol%
70	Lysine	Lys	%	Loick-Wilde, Natalie	Gas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finnigan	mol%
71	Tyrosine	Tyr	%	Loick-Wilde, Natalie	Gas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finnigan	mol%
72	Serine	Ser	%	Loick-Wilde, Natalie	Gas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finnigan	mol%
73	Threonine	Thr	%	Loick-Wilde, Natalie	Gas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finnigan	mol%
74	Glycine	Gly	%	Loick-Wilde, Natalie	Gas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finnigan	mol%

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

720 data points

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