Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

Fernández-Carrera, Ana; Steinkopf, Markus; Liskow, Iris; Wodarg, Dirk; Voss, Maren; Loick-Wilde, Natalie: Stable isotopes and mol% of amino acids in 3-200 µm seston at surface during METEOR cruise M174 [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.971279 (dataset in review)

Show MapGoogle Earth

Abstract:
The Amazon river accounts annually for 20% of riverine discharge into the global ocean with strong seasonal variations. This lens of freshwater spreads at surface as a plume, and can reach as far as the Caribbean. During this journey, the changing physicochemical characteristics of the aging plume define contrasting plankton habitats. These habitats select for communities with varying degrees of complexity of the plankton food web. During RV METEOR cruise M174 (Voss, 2021) we studied the dominant trophic function at the base of the food web (autotrophs vs mixotrophs) using stable isotopes of nitrogen in specific amino acids as well as bulk isotopes of carbon and nitrogen. This dataset contains the nitrogen signatures of 12 amino acids in δ notation (δ15N, ‰ vs N2 in air), the mol% of 12 amino acids and the carbon (δ13C, ‰ vs VPDB) and nitrogen isotopes in bulk samples at surface as well as environmental data and phytoplankton community structure. Seston was separated into two size fractions, the picoseston comprised of particles below ca. 3µm (https://doi.pangaea.de/10.1594/PANGAEA.971293), and a mixture of nano- and microseston with particles above ca. 3µm (this dataset).
Keyword(s):
Amazon River plume; amino acids mol%; Compound-specific stable isotope analyses of amino acids; seston; stable carbon isotopes δ13C; stable nitrogen isotope (δ15N)
Supplement to:
Fernández-Carrera, Ana; et al. (submitted): Mixotrophy emerges as the optimal strategy in mature waters of the Amazon River plume.
Related to:
Fernández-Carrera, Ana; Steinkopf, Markus; Liskow, Iris; Wodarg, Dirk; Voss, Maren; Loick-Wilde, Natalie: Stable isotopes and mol% of amino acids in 0.2-3 µm seston at surface during METEOR cruise M174 [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.971293
Voss, Maren (2021): Master tracks in different resolutions of METEOR cruise M174, Las Palmas - Emden, 2021-04-12 - 2021-05-29 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.935041
References:
Bricaud, Annick; Claustre, Hervé; Ras, Joséphine; Oubelkheir, Kadija (2004): Natural variability of phytoplanktonic absorption in oceanic waters: Influence of the size structure of algal populations. Journal of Geophysical Research: Oceans, 109(C11), 2004JC002419, https://doi.org/10.1029/2004JC002419
Grasshoff, Klaus; Kremling, Klaus; Ehrhardt, M (1999): Methods of Seawater Analysis. Wiley, https://doi.org/10.1002/9783527613984
Pham, Anh H; Choisnard, Noémie; Fernández-Carrera, Ana; Subramaniam, Ajit; Strope, Erica K; Carpenter, Edward J; Voss, Maren; Montoya, Joseph P (2024): Planktonic habitats in the Amazon Plume region of the Western Tropical North Atlantic. Frontiers in Marine Science, 11, 1287497, https://doi.org/10.3389/fmars.2024.1287497
Weber, Sarah C; Subramaniam, Ajit; Montoya, Joseph P; Doan-Nhu, Hai; Nguyen-Ngoc, Lam; Dippner, Joachim W; Voss, Maren (2019): Habitat Delineation in Highly Variable Marine Environments. Frontiers in Marine Science, 6, 112, https://doi.org/10.3389/fmars.2019.00112
Wright, Simon (2008): Chemtax version 1.95 for calculating the taxonomic composition of phytoplankton populations [dataset]. Australian Antarctic Data Centre, https://doi.org/10.4225/15/59FFF1C5EA8FC
Funding:
Deutsche Forschungsgemeinschaft, Bonn (DFG), grant/award no. 439440452: Metabolismus des Stickstoffs in der Amazonasfahne und dem westlichen, tropischen Nordatlantik (MeNARP)
Deutsche Forschungsgemeinschaft, Bonn (DFG), grant/award no. GPF19-1-13: N-Amazon
Coverage:
Median Latitude: 5.278214 * Median Longitude: -50.524344 * South-bound Latitude: -0.597550 * West-bound Longitude: -56.639300 * North-bound Latitude: 15.080400 * East-bound Longitude: -44.811720
Date/Time Start: 2021-04-21T07:21:11 * Date/Time End: 2021-05-13T21:57:46
Minimum Elevation: -5506.0 m * Maximum Elevation: -15.0 m
Event(s):
M174_7-4 * Latitude: 2.554400 * Longitude: -44.811720 * Date/Time: 2021-04-21T07:21:11 * Elevation: -4029.0 m * Location: North Atlantic Ocean * Campaign: M174 * Basis: Meteor (1986) * Method/Device: Water pump (PUMP)
M174_8-7 * Latitude: 1.647180 * Longitude: -45.854580 * Date/Time: 2021-04-21T18:53:15 * Elevation: -3020.0 m * Location: North Atlantic Ocean * Campaign: M174 * Basis: Meteor (1986) * Method/Device: Water pump (PUMP)
M174_9-7 * Latitude: -0.121700 * Longitude: -47.731100 * Date/Time: 2021-04-23T08:16:24 * Elevation: -36.0 m * Location: South Atlantic Ocean * Campaign: M174 * Basis: Meteor (1986) * Method/Device: Water pump (PUMP)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventLoick-Wilde, Natalie
2Station labelStationLoick-Wilde, Natalie
3CampaignCampaignLoick-Wilde, Natalie
4DATE/TIMEDate/TimeLoick-Wilde, NatalieGeocode
5LATITUDELatitudeLoick-Wilde, NatalieGeocode
6LONGITUDELongitudeLoick-Wilde, NatalieGeocode
7ELEVATIONElevationm a.s.l.Loick-Wilde, NatalieGeocode
8LocationLocationLoick-Wilde, Natalie
9HabitatHabitatLoick-Wilde, NatalieHabitat Delineation in Highly Variable Marine Environments (Weber et. al, 2019)
10DEPTH, waterDepth watermLoick-Wilde, NatalieCTD/Rosette (CTD-RO)Geocode
11SalinitySalLoick-Wilde, NatalieCTD/Rosette (CTD-RO)psu
12Temperature, waterTemp°CLoick-Wilde, NatalieCTD/Rosette (CTD-RO)
13OxygenO2µmol/lLoick-Wilde, NatalieCTD/Rosette (CTD-RO)
14SilicateSi(OH)4µmol/lLoick-Wilde, NatalieMethods of Seawater Analysis, Third Edition (Grasshoff et al., 1999)
15Nitrite[NO2]-µmol/lLoick-Wilde, NatalieMethods of Seawater Analysis, Third Edition (Grasshoff et al., 1999)
16Phosphate[PO4]3-µmol/lLoick-Wilde, NatalieMethods of Seawater Analysis, Third Edition (Grasshoff et al., 1999)
17Nitrate[NO3]-µmol/lLoick-Wilde, NatalieMethods of Seawater Analysis, Third Edition (Grasshoff et al., 1999)
18Ammonium[NH4]+µmol/lLoick-Wilde, NatalieMethods of Seawater Analysis, Third Edition (Grasshoff et al., 1999)
19Depth of nitraclineDepth nitraclinemLoick-Wilde, NatalieLinear interpolation in nutrient depth profile1µM
20Depth, phosphoclinePO4-clinemLoick-Wilde, NatalieLinear interpolation in nutrient depth profile0.1µM
21Depth, silicoclineSi-clinemLoick-Wilde, NatalieLinear interpolation in nutrient depth profile2µM
22Chlorophyll aChl aµg/lLoick-Wilde, NatalieHigh performance liquid chromatography (HPLC), Agilent 1200
23High-pressure liquid chromatography size indexHPLC Size indexµmLoick-Wilde, NatalieBased on HPLC pigments according to Bricaud et al (2004)
24River plume thicknessRiver plume thicknessmLoick-Wilde, NatalieLinear interpolation depth of 35 PSU
25Brunt-Väisälä frequency, maximumN2 max1/s2Loick-Wilde, Natalie
26Mixed layer depthMLDmLoick-Wilde, Natalie
27Depth, deep chlorophyll maximumDCM depthmLoick-Wilde, Natalie
28Depth, 1% photosynthetically active radiation1% PAR depthmLoick-Wilde, Natalie
29Filamentous CyanobacteriaFilamentous cyanob%Loick-Wilde, NatalieChemTax v 1.9.5 (Wright, S., 2017)
30SynechococcusSynechococcus%Loick-Wilde, NatalieChemTax v 1.9.5 (Wright, S., 2017)
31ProchlorococcusProchlorococcus%Loick-Wilde, NatalieChemTax v 1.9.5 (Wright, S., 2017)
32PrasinophytesPrasinophytes%Loick-Wilde, NatalieChemTax v 1.9.5 (Wright, S., 2017)
33CryptophytesCryptophytes%Loick-Wilde, NatalieChemTax v 1.9.5 (Wright, S., 2017)
34DiatomsDiatoms%Loick-Wilde, NatalieChemTax v 1.9.5 (Wright, S., 2017)
35HaptophytesHaptophytes%Loick-Wilde, NatalieChemTax v 1.9.5 (Wright, S., 2017)
36DinoflagellatesDinofl%Loick-Wilde, NatalieChemTax v 1.9.5 (Wright, S., 2017)
37Carbon, particulatePCµmol/lLoick-Wilde, NatalieElemental analyzer, Thermo Finnigan, 1112 Flash; coupled with Isotope ratio mass spectrometer, Thermo Finnigan, Delta Plus; continuous-flow carrier-gas system, Thermo Finnigan, ConFlo III
38δ13C, sestonδ13C sest‰ PDBLoick-Wilde, NatalieElemental analyzer, Thermo Finnigan, 1112 Flash; coupled with Isotope ratio mass spectrometer, Thermo Finnigan, Delta Plus; continuous-flow carrier-gas system, Thermo Finnigan, ConFlo III
39Nitrogen, particulatePNµmol/lLoick-Wilde, NatalieElemental analyzer, Thermo Finnigan, 1112 Flash; coupled with Isotope ratio mass spectrometer, Thermo Finnigan, Delta Plus; continuous-flow carrier-gas system, Thermo Finnigan, ConFlo III
40δ15N, sestonδ15N sest‰ airLoick-Wilde, NatalieElemental analyzer, Thermo Finnigan, 1112 Flash; coupled with Isotope ratio mass spectrometer, Thermo Finnigan, Delta Plus; continuous-flow carrier-gas system, Thermo Finnigan, ConFlo III
41Glutamine and Glutamic acid, δ15NGln + Glu δ15N‰ airLoick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
42Glutamine and Glutamic acid, δ15N standard deviationGln + Glu δ15N std dev±Loick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
43Phenylalanine, δ15NPhe δ15N‰ airLoick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
44Phenylalanine, δ15N, standard deviationPhe δ15N std dev±Loick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
45Alanine, δ15NAla δ15N‰ airLoick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
46Alanine, δ15N, standard deviationAla δ15N std dev±Loick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
47Valine, δ15NVal δ15N‰ airLoick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
48Valine, δ15N, standard deviationVal δ15N std dev±Loick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
49Leucine, δ15NLeu δ15N‰ airLoick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
50Leucine, δ15N, standard deviationLeu δ15N std dev±Loick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
51Isoleucine, δ15NIle δ15N‰ airLoick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
52Isoleucine, δ15N, standard deviationIle δ15N std dev±Loick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
53Proline, δ15NPro δ15N‰ airLoick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
54Proline, δ15N, standard deviationPro δ15N std dev±Loick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
55Aspartamine and Aspartic acid, δ15NAsn + Asp δ15N‰ airLoick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
56Aspartamine and Aspartic acid, δ15N standard deviationAsn + Asp δ15N std dev±Loick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
57Lysine, δ15NLys δ15N‰ airLoick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
58Lysine, δ15N, standard deviationLys δ15N std dev±Loick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
59Tyrosine, δ15NTyr δ15N‰ airLoick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
60Tyrosine, δ15N standard deviationTyr δ5N std dev±Loick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
61Serine, δ15NSer δ15N‰ airLoick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
62Serine, δ15N, standard deviationSer δ15N std dev±Loick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
63Threonine, δ15NThr δ15N‰ airLoick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
64Threonine, δ15N, standard deviationThr δ15N std dev±Loick-Wilde, NatalieIsotope ration mass spectrometer (IRMS), Thermo Fischer Scientific, MAT253; universal continuous flow interface, Thermo Scientific, ConFlo IV; gas chromatography, Thermo Scientific, GC Isolink II; gas chromatography, Thermo Scientific, Trace GC 1300
65Glutamine and Glutamic acidGln + Glu%Loick-Wilde, NatalieGas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finniganmol%
66PhenylalaninePhe%Loick-Wilde, NatalieGas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finniganmol%
67AlanineAla%Loick-Wilde, NatalieGas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finniganmol%
68ValineVal%Loick-Wilde, NatalieGas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finniganmol%
69LeucineLeu%Loick-Wilde, NatalieGas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finniganmol%
70IsoleucineIle%Loick-Wilde, NatalieGas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finniganmol%
71ProlinePro%Loick-Wilde, NatalieGas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finniganmol%
72Aspartamine and Aspartic acidAsn + Asp%Loick-Wilde, NatalieGas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finniganmol%
73LysineLys%Loick-Wilde, NatalieGas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finniganmol%
74TyrosineTyr%Loick-Wilde, NatalieGas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finniganmol%
75SerineSer%Loick-Wilde, NatalieGas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finniganmol%
76ThreonineThr%Loick-Wilde, NatalieGas chromatographer mass spectrometer, Thermo Finnigan, Trace GCMS DSQ II; coupled with TriPlus Autosampler, Thermo Finniganmol%
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1172 data points

Download Data (login required; moratorium until 2026-01-31)

Download dataset as tab-delimited text — use the following character encoding:

View dataset as HTML