Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

Venn, Alexander A; Techer, Nathalie; Segonds, Natacha; Tambutté, Eric; Tambutté, Sylvie: Indo--1 Fluorescence and intracellular Ca2+ concentration in solution of Stylophora pistillata laboratory experiment [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.968789 (dataset in review)

Show MapGoogle Earth

Abstract:
The fluorescence of the calcium dye Indo-1 was measured in solution by confocal microscopy at different calcium concentrations. A calibration curve was produced from the ratio of Indo-1 fluoresence at different calcium concentrations. A dissociation constant for indo-1 and calcium was derived from the calibration curve.
Keyword(s):
calcium; Cell biology; Coral
Supplement to:
Venn, Alexander A; Techer, Nathalie; Segonds, Natacha; Tambutté, Eric; Tambutté, Sylvie (2024): Quantification of cytosolic 'free' calcium in isolated coral cells with confocal microscopy. Journal of Experimental Biology, jeb.247638, https://doi.org/10.1242/jeb.247638
Coverage:
Latitude: 43.738418 * Longitude: 7.424616
Event(s):
Stylophora_pistillata_experiment * Latitude: 43.738418 * Longitude: 7.424616 * Method/Device: Laboratory experiment * Comment: Stylophora pistillata is a species cultured for many years at the Centre Scientifique de Monaco.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Type of studyStudy typeVenn, Alexander A
2FigureFigVenn, Alexander A
3Experimental treatmentExp treatVenn, Alexander A
4Treatment: temperatureT:temp°CVenn, Alexander AThermometer, Ponsel
5Treatment: salinityT:salVenn, Alexander A
6Stylophora pistillata, position in depthP. pistillata z depthµmVenn, Alexander AConfocal Microscope, Leica, SP8Supplimentary data in the associated manuscript
7Indo-1 calcium indicator, fluorescence at 420-440 nmIndo-1 420-440arbitrary unitsVenn, Alexander AConfocal Microscope, Leica, SP8Supplimentary data in the associated manuscript
8Indo-1 calcium indicator, fluorescence at 430-450 nmIndo-1 430-450arbitrary unitsVenn, Alexander AConfocal Microscope, Leica, SP8Supplimentary data in the associated manuscript
9log-ratiolog-ratioVenn, Alexander ACalculated
10Calcium (centered log ratio)Ca (CLR)Venn, Alexander ACalculated
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
191 data points

Download Data (login required; moratorium until 2026-06-05)

Download dataset as tab-delimited text — use the following character encoding:

View dataset as HTML