Venn, Alexander A; Techer, Nathalie; Segonds, Natacha; Tambutté, Eric; Tambutté, Sylvie: Indo--1 Fluorescence and intracellular Ca2+ concentration in solution of Stylophora pistillata laboratory experiment [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.968789 (dataset in review)
Abstract:
The fluorescence of the calcium dye Indo-1 was measured in solution by confocal microscopy at different calcium concentrations. A calibration curve was produced from the ratio of Indo-1 fluoresence at different calcium concentrations. A dissociation constant for indo-1 and calcium was derived from the calibration curve.
Supplement to:
Venn, Alexander A; Techer, Nathalie; Segonds, Natacha; Tambutté, Eric; Tambutté, Sylvie (2024): Quantification of cytosolic 'free' calcium in isolated coral cells with confocal microscopy. Journal of Experimental Biology, jeb.247638, https://doi.org/10.1242/jeb.247638
Coverage:
Latitude: 43.738418 * Longitude: 7.424616
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Venn, Alexander A | |||
| 2 | Figure | Fig | Venn, Alexander A | |||
| 3 | Experimental treatment | Exp treat | Venn, Alexander A | |||
| 4 | Treatment: temperature | T:temp | °C | Venn, Alexander A | Thermometer, Ponsel | |
| 5 | Treatment: salinity | T:sal | Venn, Alexander A | |||
| 6 | Stylophora pistillata, position in depth | P. pistillata z depth | µm | Venn, Alexander A | Confocal Microscope, Leica, SP8 | Supplimentary data in the associated manuscript |
| 7 | Indo-1 calcium indicator, fluorescence at 420-440 nm | Indo-1 420-440 | arbitrary units | Venn, Alexander A | Confocal Microscope, Leica, SP8 | Supplimentary data in the associated manuscript |
| 8 | Indo-1 calcium indicator, fluorescence at 430-450 nm | Indo-1 430-450 | arbitrary units | Venn, Alexander A | Confocal Microscope, Leica, SP8 | Supplimentary data in the associated manuscript |
| 9 | log-ratio | log-ratio | Venn, Alexander A | Calculated | ||
| 10 | Calcium (centered log ratio) | Ca (CLR) | Venn, Alexander A | Calculated |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
191 data points