Citation:

PANGAEA.968788 (dataset in review)

Name: Quantification of intracellular 'free' calcium in isolated coral cells of Stylophora pistillata in laboratory experiment
License: https://creativecommons.org/licenses/by/4.0/
Keywords: calcium; Cell biology; Coral

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Abstract:

Despite its prominent role as an intracellular messenger in all organisms, intracellular free calcium ([Ca2+]i) has not been quantified in corals or cnidarians in general. Ratiometric calcium dyes and cell imaging have been key methods in successful research on [Ca2+]i in mammlian and plat model systems, and could be applied to corals. Here, we developed a procedure to quantity [Ca2+]i in isolated cells from the model coral species Stylophora pistillata using Indo-1 and confocal microscopy mainatined in aquarium culture at the Centre Scientifique de Monaco, Monaco between 2020 and 2023. We quantified [Ca2+]i in coral cells with and without intracellular dinoflagellate symbionts, and verified our procedure on cultured mammalian cells. We then used our procedure to measure changes in [Ca2+]i in coral cells exposed to the classic inhibitor of [Ca2+], thapsigargin, and also used it to record elevations in [Ca2+]i in coral cells undergoing apoptosis. Our procedure paves the way for future studies into intracellular calcium in corals and other cnidarians.

Keyword(s):

calcium; Cell biology; Coral

Supplement to:

Venn, Alexander A; Techer, Nathalie; Segonds, Natacha; Tambutté, Eric; Tambutté, Sylvie (2024): Quantification of cytosolic 'free' calcium in isolated coral cells with confocal microscopy. Journal of Experimental Biology, jeb.247638, https://doi.org/10.1242/jeb.247638

Coverage:

Latitude: 43.738418 * Longitude: 7.424616

Event(s):

Stylophora_pistillata_experiment * Latitude: 43.738418 * Longitude: 7.424616 * Method/Device: Laboratory experiment * Comment: Stylophora pistillata is a species cultured for many years at the Centre Scientifique de Monaco.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Venn, Alexander A
2	Figure	Fig		Venn, Alexander A
3	Experimental treatment	Exp treat		Venn, Alexander A
4	Treatment: temperature	T:temp	°C	Venn, Alexander A	Thermometer, Ponsel
5	Treatment: salinity	T:sal		Venn, Alexander A
6	Stylophora pistillata, symbiont containing cells, intracellular calcium	P. pistillata SC cell [Ca2+] in	nmol/l	Venn, Alexander A	Confocal Microscope, Leica, SP8	Figure 2 in the associated manuscript
7	Stylophora pistillata, symbiont containing cells, intracellular calcium, standard deviation	P. pistillata SC cell [Ca2+] in std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP8	Figure 2 in the associated manuscript
8	Stylophora pistillata, annexin V positive symbiont containing cells, intracellular calcium	P. pistillata ann+SC [Ca2+] in	nmol/l	Venn, Alexander A	Confocal Microscope, Leica, SP8	Figure 3 in the associated manuscript
9	Stylophora pistillata, annexin V positive symbiont containing cells, intracellular calcium, standard deviation	P. pistillata ann+SC [Ca2+] in std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP8	Figure 3 in the associated manuscript
10	Stylophora pistillata, annexin V positive positive symbiont free cells, intracellular calcium	P. pistillata ann+SF [Ca2+] in	nmol/l	Venn, Alexander A	Confocal Microscope, Leica, SP8	Figure 3 in the associated manuscript
11	Stylophora pistillata, annexin V positive positive symbiont free cells, intracellular calcium, standard deviation	P. pistillata ann+SF [Ca2+] in std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP8	Figure 3 in the associated manuscript
12	Stylophora pistillata, annexin V negative symbiont containing cells, intracellular calcium	P. pistillata ann-SC [Ca2+] in	nmol/l	Venn, Alexander A	Confocal Microscope, Leica, SP8	Figure 3 in the associated manuscript
13	Stylophora pistillata, annexin V negative symbiont containing cells, intracellular calcium, standard deviation	P. pistillata ann-SC [Ca2+] in std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP8	Figure 3 in the associated manuscript
14	Stylophora pistillata, annexin V negative symbiont free cells, intracellular calcium	P. pistillata ann-SF [Ca2+] in	nmol/l	Venn, Alexander A	Confocal Microscope, Leica, SP8	Figure 3 in the associated manuscript
15	Stylophora pistillata, annexin V negative symbiont free cells, intracellular calcium, standard deviation	P. pistillata ann-SF [Ca2+] in std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP8	Figure 3 in the associated manuscript

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

41 data points

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