Piwosz, Kasia: Response of Aerobic Anoxygenic Phototrophic bacteria to carbon limitation [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.967435 (dataset in review)
Abstract:
Aerobic Anoxygenic Phototrophic (AAP) bacteria are an important component of freshwater bacterioplankton. They can support their heterotrophic metabolism with energy from light, and by that enhance their growth efficiency. Based on results from cultures, it was hypothesized that photoheterotrophy provides an advantage under carbon limitation and facilitates access to recalcitrant or low-energy carbon sources. However, verification of these hypotheses for natural AAP communities has been lacking. Here, we conducted whole community manipulation experiments and compared the growth of AAP bacteria under carbon limited and with recalcitrant (lignin) or low energy (acetate) carbon sources under dark and infra-red (IR) light conditions to elucidate how they profit from photoheterotrophy. We performed two whole community manipulation experiments in 2-litre microcosms. The first experiment was conducted in June 2018. Lignin was used as the recalcitrant carbon source. Samples were collected every 12 hours. The second experiment was conducted in October 2018. Acetate was used as the low-energy carbon source. Samples were collected every 24 hours. Both experiments were done in at in situ temperature. We measured concentrations of bacteriochlorophyll-a, total prokaryotic count, aerobic anoxygenic phototrophic bacteria count, and heterotrophic nanoflagellates count. This dataset is related to Piwosz et al. (in prep).
Funding:
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Piwosz, Kasia | |||
| 2 | Date/time start, experiment | Date/time start exp | Piwosz, Kasia | |||
| 3 | Duration | Duration | Piwosz, Kasia | Of experiment | ||
| 4 | Sample code/label | Sample label | Piwosz, Kasia | |||
| 5 | Experimental condition | Exp condition | Piwosz, Kasia | Ingoranic basal medium dilution according to Hahn et al. (20023) | A priori defined experimental conditions at the start of the experiment: control: 1:4 dilution with sterile-filtered lake water, C-limitation: 1:4 dilution with inorganic basal medium; Ligning: 1:4 dilution with inorganic basal medium amended with 2.5 mg per L of Lignin, Acetate: 1:4 dilution with inorganic basal medium amended with 3.0 mg per L of sodium acetate. | |
| 6 | Treatment: light condition | T:light | Piwosz, Kasia | A priori defined light conditions at the start of the expriment: dark: no light; IR: infrared light of wavelength > 800 nm. | ||
| 7 | Replicate | Repl | Piwosz, Kasia | |||
| 8 | Treatment: temperature | T:temp | °C | Piwosz, Kasia | Mercury thermometer | |
| 9 | Bacteriochlorophyll a | BChl a | nmol/l | Piwosz, Kasia | Fluorometer, PSI (Photon Systems Instruments), spol. s.r.o., FL200/PS | |
| 10 | Prokaryotes | Prokaryotes | #/ml | Piwosz, Kasia | Microscope, Carl Zeiss, Axio Imager.D2; coupled with Collibri LED module illumination system, Carl Zeiss | Total cell counts. Further analysis methods: Coleman (1980, doi:10.4319/lo.1980.25.5.0948). |
| 11 | Bacteria, aerobic, anoxygenic, phototrophic | AAPB | #/ml | Piwosz, Kasia | Microscope, Carl Zeiss, Axio Imager.D2; coupled with Collibri LED module illumination system, Carl Zeiss | Further analysis methods: Cottrell et al. (2006, doi:10.1128/AEM.72.1.557-564.2006) and Piwosz et al. (2022, doi:10.1038/s41396-021-01142-2). |
| 12 | Nanoflagellates, heterotrophic | HNF | #/ml | Piwosz, Kasia | Microscope, Carl Zeiss, Axio Imager.D2; coupled with Collibri LED module illumination system, Carl Zeiss | Further analysis methods: Sherr et al. (1989, doi:10.3354/meps054209). |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2156 data points
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