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Piwosz, Kasia (2024): Response of Aerobic Anoxygenic Phototrophic bacteria to carbon limitation [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.967435

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Published: 2024-06-27 • DOI registered: 2024-06-27

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Abstract:
Aerobic Anoxygenic Phototrophic (AAP) bacteria are an important component of freshwater bacterioplankton. They can support their heterotrophic metabolism with energy from light, and by that enhance their growth efficiency. Based on results from cultures, it was hypothesized that photoheterotrophy provides an advantage under carbon limitation and facilitates access to recalcitrant or low-energy carbon sources. However, verification of these hypotheses for natural AAP communities has been lacking. Here, we conducted whole community manipulation experiments and compared the growth of AAP bacteria under carbon limited and with recalcitrant (lignin) or low energy (acetate) carbon sources under dark and infra-red (IR) light conditions to elucidate how they profit from photoheterotrophy. We performed two whole community manipulation experiments in 2-litre microcosms. The first experiment was conducted in June 2018. Lignin was used as the recalcitrant carbon source. Samples were collected every 12 hours. The second experiment was conducted in October 2018. Acetate was used as the low-energy carbon source. Samples were collected every 24 hours. Both experiments were done in at in situ temperature. We measured concentrations of bacteriochlorophyll-a, total prokaryotic count, aerobic anoxygenic phototrophic bacteria count, and heterotrophic nanoflagellates count. This dataset is related to Piwosz et al. (in prep).
Supplement to:
Piwosz, Kasia; Villena-Alemany, Cristian; Całkiewicz, Joanna; Mujakić, Izabela; Náhlík, Vít; Dean, Jason; Koblížek, Michal (2024): Response of aerobic anoxygenic phototrophic bacteria to limitation and availability of organic carbon. FEMS Microbiology Ecology, fiae090, https://doi.org/10.1093/femsec/fiae090
Funding:
National Science Center - Poland (NCN), grant/award no. 2021/03/Y/NZ8/00076
National Science Center - Poland (NCN), grant/award no. 2021/42/E/NZ8/00163
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 Type of studyStudy typePiwosz, Kasia
2 Date/time start, experimentDate/time start expPiwosz, Kasia
3 DurationDurationPiwosz, KasiaOf experiment
4 Sample code/labelSample labelPiwosz, Kasia
5 Experimental conditionExp conditionPiwosz, KasiaIngoranic basal medium dilution according to Hahn et al. (20023)A priori defined experimental conditions at the start of the experiment: control: 1:4 dilution with sterile-filtered lake water, C-limitation: 1:4 dilution with inorganic basal medium; Ligning: 1:4 dilution with inorganic basal medium amended with 2.5 mg per L of Lignin, Acetate: 1:4 dilution with inorganic basal medium amended with 3.0 mg per L of sodium acetate.
6 Treatment: light conditionT:lightPiwosz, KasiaA priori defined light conditions at the start of the expriment: dark: no light; IR: infrared light of wavelength > 800 nm.
7 ReplicateReplPiwosz, Kasia
8 Treatment: temperatureT:temp°CPiwosz, KasiaMercury thermometer
9 Bacteriochlorophyll aBChl anmol/lPiwosz, KasiaFluorometer, PSI (Photon Systems Instruments), spol. s.r.o., FL200/PS
10 ProkaryotesProkaryotes#/mlPiwosz, KasiaMicroscope, Carl Zeiss, Axio Imager.D2; coupled with Collibri LED module illumination system, Carl ZeissTotal cell counts. Further analysis methods: Coleman (1980, doi:10.4319/lo.1980.25.5.0948).
11 Bacteria, aerobic, anoxygenic, phototrophicAAPB#/mlPiwosz, KasiaMicroscope, Carl Zeiss, Axio Imager.D2; coupled with Collibri LED module illumination system, Carl ZeissFurther analysis methods: Cottrell et al. (2006, doi:10.1128/AEM.72.1.557-564.2006) and Piwosz et al. (2022, doi:10.1038/s41396-021-01142-2).
12 Nanoflagellates, heterotrophicHNF#/mlPiwosz, KasiaMicroscope, Carl Zeiss, Axio Imager.D2; coupled with Collibri LED module illumination system, Carl ZeissFurther analysis methods: Sherr et al. (1989, doi:10.3354/meps054209).
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2156 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Study type		2 
Date/time start exp		3 
Duration		4 
Sample label		5 
Exp condition		6 
T:light		7 
Repl		8 
T:temp [°C]		9 
BChl a [nmol/l]		10 
Prokaryotes [#/ml]		11 
AAPB [#/ml]		12 
HNF [#/ml]
Laboratory experiment2018-06-20T18:000 hrs 0 min1ControlDarkA21.011.613482791564601158
Laboratory experiment2018-06-20T18:000 hrs 0 min2ControlDarkB22.011.51536939193204826
Laboratory experiment2018-06-20T18:000 hrs 0 min3ControlDarkC23.011.71550372162386878
Laboratory experiment2018-06-20T18:000 hrs 0 min4ControlIRA21.011.81077545137495709
Laboratory experiment2018-06-20T18:000 hrs 0 min5ControlIRB22.011.412485161410511247
Laboratory experiment2018-06-20T18:000 hrs 0 min6ControlIRC23.010.51300274169498912
Laboratory experiment2018-06-20T12:150 hrs 0 min7DilutionDarkA21.010.71536939193204208
Laboratory experiment2018-06-20T12:150 hrs 0 min8DilutionDarkB22.010.81077545137495270
Laboratory experiment2018-06-20T12:150 hrs 0 min9DilutionDarkC23.010.51248516141051385
Laboratory experiment2018-06-20T12:150 hrs 0 min10DilutionIRA21.010.71348279156460306
Laboratory experiment2018-06-20T12:150 hrs 0 min11DilutionIRB22.010.61536939193204433
Laboratory experiment2018-06-20T12:150 hrs 0 min12DilutionIRC23.010.01550372162386584
Laboratory experiment2018-06-20T12:400 hrs 0 min13LigninDarkA21.010.01536939193204426
Laboratory experiment2018-06-20T12:400 hrs 0 min14LigninDarkB22.010.11077545137495496
Laboratory experiment2018-06-20T12:400 hrs 0 min15LigninDarkC23.010.11348279156460
Laboratory experiment2018-06-20T12:400 hrs 0 min16LigninIRA21.010.01300274169498567
Laboratory experiment2018-06-20T12:400 hrs 0 min17LigninIRB22.010.01550372162386347
Laboratory experiment2018-06-20T12:400 hrs 0 min18LigninIRC23.09.81608057196760483
Laboratory experiment2018-06-21T08:1514 hrs 15 min19ControlDarkA22.016.513334632927691045
Laboratory experiment2018-06-21T08:1514 hrs 15 min20ControlDarkB21.216.312184882714341450
Laboratory experiment2018-06-21T08:1514 hrs 15 min21ControlDarkC21.217.212063722975101217
Laboratory experiment2018-06-21T08:1514 hrs 15 min22ControlIRA22.017.21179374216910
Laboratory experiment2018-06-21T08:1514 hrs 15 min23ControlIRB21.217.612276322003161491
Laboratory experiment2018-06-21T08:1514 hrs 15 min24ControlIRC21.218.211917652015011055
Laboratory experiment2018-06-21T08:1520 hrs 0 min25DilutionDarkA22.09.471381480600847
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Laboratory experiment2018-06-21T08:1520 hrs 0 min29DilutionIRB21.28.232240282971275
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Laboratory experiment2018-06-21T20:1526 hrs 15 min42ControlIRC19.019.91398654359146545
Laboratory experiment2018-06-21T20:1532 hrs 0 min43DilutionDarkA19.015.11096403207428364
Laboratory experiment2018-06-21T20:1532 hrs 0 min44DilutionDarkB19.014.91255233257210370
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Laboratory experiment2018-06-21T20:1531 hrs 35 min49LigninDarkA19.010.51519339279731798
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Laboratory experiment2018-06-21T20:1531 hrs 35 min53LigninIRB19.09.81029518201501547
Laboratory experiment2018-06-21T20:1531 hrs 35 min54LigninIRC19.010.0934016182536
Laboratory experiment2018-06-22T08:1538 hrs 15 min55ControlDarkA19.022.72019620447451285
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Laboratory experiment2018-06-22T08:1538 hrs 15 min58ControlIRA19.022.72003157440932175
Laboratory experiment2018-06-22T08:1538 hrs 15 min59ControlIRB19.023.51706832352034453
Laboratory experiment2018-06-22T08:1538 hrs 15 min60ControlIRC19.023.11854995396483389
Laboratory experiment2018-06-22T08:1544 hrs 0 min61DilutionDarkA19.017.91333463278546168
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Laboratory experiment2018-06-22T08:1544 hrs 0 min66DilutionIRC19.020.71262345311734462
Laboratory experiment2018-06-22T08:1543 hrs 35 min67LigninDarkA19.012.01309757277360716
Laboratory experiment2018-06-22T08:1543 hrs 35 min68LigninDarkB19.012.21206635245357303
Laboratory experiment2018-06-22T08:1543 hrs 35 min69LigninDarkC19.011.91133443225207411
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Laboratory experiment2018-06-22T08:1543 hrs 35 min72LigninIRC19.011.048597382971487
Laboratory experiment2018-06-22T20:1550 hrs 15 min73ControlDarkA19.022.81964211336625289
Laboratory experiment2018-06-22T20:1550 hrs 15 min74ControlDarkB19.022.92019751318846175
Laboratory experiment2018-06-22T20:1550 hrs 15 min75ControlDarkC19.022.22515799373370202
Laboratory experiment2018-06-22T20:1550 hrs 15 min76ControlIRA19.020.11711912482417200
Laboratory experiment2018-06-22T20:1550 hrs 15 min77ControlIRB19.020.52209738581982346
Laboratory experiment2018-06-22T20:1550 hrs 15 min78ControlIRC19.020.92408530606874158
Laboratory experiment2018-06-22T20:1556 hrs 0 min79DilutionDarkA19.019.91672590407743108
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Laboratory experiment2018-06-22T20:1556 hrs 0 min83DilutionIRB19.021.71626824462267211
Laboratory experiment2018-06-22T20:1556 hrs 0 min84DilutionIRC19.020.71252467352034298
Laboratory experiment2018-06-22T20:1555 hrs 35 min85LigninDarkA19.015.31745778335440228
Laboratory experiment2018-06-22T20:1555 hrs 35 min86LigninDarkB19.015.41406556274990368
Laboratory experiment2018-06-22T20:1555 hrs 35 min87LigninDarkC19.014.31676661337811264
Laboratory experiment2018-06-22T20:1555 hrs 35 min88LigninIRA19.012.81151717169498352
Laboratory experiment2018-06-22T20:1555 hrs 35 min89LigninIRB19.011.91238639232319278
Laboratory experiment2018-06-22T20:1555 hrs 35 min90LigninIRC19.013.01512443256025255
Laboratory experiment2018-10-01T12:000 hrs 0 min145ControlDarkA16.51.4101461793639367
Laboratory experiment2018-10-01T12:000 hrs 0 min146ControlDarkB16.54.996839059265499
Laboratory experiment2018-10-01T12:000 hrs 0 min147ControlDarkC16.54.7110707050968472
Laboratory experiment2018-10-01T12:000 hrs 0 min148ControlIRA16.53.285578748597460
Laboratory experiment2018-10-01T12:000 hrs 0 min149ControlIRB16.51.981311636744453
Laboratory experiment2018-10-01T12:000 hrs 0 min150ControlIRC16.54.382496939115643
Laboratory experiment2018-10-01T12:000 hrs 0 min151DilutionDarkA16.52.286882542671256
Laboratory experiment2018-10-01T12:000 hrs 0 min152DilutionDarkB16.51.479415158080188
Laboratory experiment2018-10-01T12:000 hrs 0 min153DilutionDarkC16.51.692334956894163
Laboratory experiment2018-10-01T12:000 hrs 0 min154DilutionIRA16.54.7109284753339229
Laboratory experiment2018-10-01T12:000 hrs 0 min155DilutionIRB16.51.7108573547412101
Laboratory experiment2018-10-01T12:000 hrs 0 min156DilutionIRC16.51.890438440300264
Laboratory experiment2018-10-01T12:000 hrs 0 min157AcetateDarkA16.51.5105136137930409
Laboratory experiment2018-10-01T12:000 hrs 0 min158AcetateDarkB16.51.5124575068747472
Laboratory experiment2018-10-01T12:000 hrs 0 min159AcetateDarkC16.54.796483443856366
Laboratory experiment2018-10-01T12:000 hrs 0 min160AcetateIRA16.52.2109403274674343
Laboratory experiment2018-10-01T12:000 hrs 0 min161AcetateIRB16.51.687475158080375
Laboratory experiment2018-10-01T12:000 hrs 0 min162AcetateIRC16.51.5109758858080355
Laboratory experiment2018-10-02T12:1524 hrs 0 min163ControlDarkA16.50.61277753129198205
Laboratory experiment2018-10-02T12:1524 hrs 0 min164ControlDarkB16.51.11479254149348222
Laboratory experiment2018-10-02T12:1524 hrs 0 min165ControlDarkC16.51.11536149152904186
Laboratory experiment2018-10-02T12:1524 hrs 0 min166ControlIRA16.50.91395098110233241
Laboratory experiment2018-10-02T12:1524 hrs 0 min167ControlIRB16.51.31305015119715175
Laboratory experiment2018-10-02T12:1524 hrs 0 min168ControlIRC16.51.92105093177795296
Laboratory experiment2018-10-02T12:1524 hrs 0 min169DilutionDarkA16.51.41585931120901227
Laboratory experiment2018-10-02T12:1524 hrs 0 min170DilutionDarkB16.50.91411692107862281
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Laboratory experiment2018-10-02T12:1524 hrs 0 min172DilutionIRA16.51.71657049149348228
Laboratory experiment2018-10-02T12:1524 hrs 0 min173DilutionIRB16.53.01389172136310361
Laboratory experiment2018-10-02T12:1524 hrs 0 min174DilutionIRC16.51.5138680198380171
Laboratory experiment2018-10-02T12:1524 hrs 0 min175AcetateDarkA16.51.91405766109048237
Laboratory experiment2018-10-02T12:1524 hrs 0 min176AcetateDarkB16.51.6136072493639254
Laboratory experiment2018-10-02T12:1524 hrs 0 min177AcetateDarkC16.51.3138798672303347
Laboratory experiment2018-10-02T12:1524 hrs 0 min178AcetateIRA16.51.41815880182536266
Laboratory experiment2018-10-02T12:1524 hrs 0 min179AcetateIRB16.51.71436584132754278
Laboratory experiment2018-10-02T12:1524 hrs 0 min180AcetateIRC16.51.01531408136310200
Laboratory experiment2018-10-03T12:0048 hrs 0 min181ControlDarkA16.53.5143184273489345
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Laboratory experiment2018-10-03T12:0048 hrs 0 min184ControlIRA16.54.11387986107862318
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Laboratory experiment2018-10-03T12:0048 hrs 0 min186ControlIRC16.53.4141169275859374
Laboratory experiment2018-10-03T12:0048 hrs 0 min187DilutionDarkA16.54.51563411119715336
Laboratory experiment2018-10-03T12:0048 hrs 0 min188DilutionDarkB16.54.4150059099565341
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Laboratory experiment2018-10-03T12:0048 hrs 0 min192DilutionIRC16.53.7147332894824308
Laboratory experiment2018-10-03T12:0048 hrs 0 min193AcetateDarkA16.53.91539705143421274
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Laboratory experiment2018-10-04T12:0072 hrs 0 min215AcetateIRB16.55.5139509864006192
Laboratory experiment2018-10-04T12:0072 hrs 0 min216AcetateIRC16.54.7123034167562184
Laboratory experiment2018-10-05T12:0096 hrs 0 min217ControlDarkA16.54.7144606666377341
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Laboratory experiment2018-10-05T12:0096 hrs 0 min220ControlIRA16.54.3127656842671411
Laboratory experiment2018-10-05T12:0096 hrs 0 min221ControlIRB16.54.5169023856894472
Laboratory experiment2018-10-05T12:0096 hrs 0 min222ControlIRC16.54.5179217455709457
Laboratory experiment2018-10-05T12:0096 hrs 0 min223DilutionDarkA16.515.62012639348478317
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Laboratory experiment2018-10-05T12:0096 hrs 0 min229AcetateDarkA16.58.52313706138680142
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Laboratory experiment2018-10-05T12:0096 hrs 0 min234AcetateIRC16.54.9121967436744128