Ahme, Antonia; Happe, Anika; Striebel, Maren; Olsson, Markus; Giesler, Jakob; Kline, Simon; John, Uwe (2023): Thermal performance assay of a plankton community from Helgoland Roads for an indoor mesocosm experiment in March 2022 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.961508
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Abstract:
To choose the treatment temperatures for an indoor mesocosm temperature experiment at the ICBM in Wilhelmshaven (https://doi.pangaea.de/10.1594/PANGAEA.961155), a thermal performance curve assay was performed from the 8th of March until the 16th of March. It was started one day after filling the mesocosms with seawater from Helgoland Roads (https://deims.org/1e96ef9b-0915-4661-849f-b3a72f5aa9b1) by randomly spreading pooled sample water in 50 ml culture flasks across ten temperatures (3 °C to 30 °C in 3 °C steps) in triplicates. Their fluorescence (395/680 Excitation/Emission) was measured daily using a SYNERGY H1 microplate reader (BioTek, Winooski, Vermont, USA).
Keyword(s):
Related to:
Ahme, Antonia; Happe, Anika; Striebel, Maren; Olsson, Markus; Kühne, Nancy; Giesler, Jakob; Sentimenti, Alexander; Schulte-Hillen, Ruben L; Cabrerizo, Marco J; Kline, Simon; Neun, Sebastian; Naus-Wiezer, Suzanne M; Rickels, Heike; Van de Waal, Dedmer B; Torres-Valdés, Sinhué; John, Uwe (2023): Temperature effects on a plankton community from Helgoland Roads tested in an indoor mesocosm experiment in March 2022. PANGAEA, https://doi.org/10.1594/PANGAEA.961155
References:
Ahme, Antonia; Happe, Anika; Striebel, Maren; Olsson, Markus; Kühne, Nancy; Giesler, Jakob; Sentimenti, Alexander; Schulte-Hillen, Ruben L; Cabrerizo, Marco J; Kline, Simon; Neun, Sebastian; Naus-Wiezer, Suzanne M; Rickels, Heike; Van de Waal, Dedmer B; Torres-Valdés, Sinhué; John, Uwe (accepted): 18S-metabarcoding data of an indoor mesocosm experiment testing the effect of temperature on a plankton community from Helgoland Roads, March 2022. European Nucleotide Archive (ENA), insdc:PRJEB72441
Gall, Andrea; Uebel, Udo; Ebensen, Uwe; Hillebrand, Helmut; Meier, Sandra; Singer, Gabriel; Wacker, Alexander; Striebel, Maren (2017): Planktotrons: A novel indoor mesocosm facility for aquatic biodiversity and food web research. Limnology and Oceanography-Methods, 15(7), 663-677, https://doi.org/10.1002/lom3.10196
Related code / software:
Ahme, Antonia (2023): Data analysis R scripts for a mesocosm incubation experiment to assess the effect of temperature on a plankton community from Helgoland Roads. https://github.com/AntoniaAhme/TopTronsMesocosmIncubation
Project(s):
Changing Earth – Sustaining our Future (Helmholtz_ChangingEarth)
Funding:
Helmholtz Association of German Research Centres (HGF), grant/award no. ChangingEarth_Subtopic_6.2: Adaptation of marine life: From genes to ecosystems
Horizon 2020 (H2020), grant/award no. 871081: AQUACOSM-plus: Network of Leading Ecosystem Scale Experimental AQUAtic MesoCOSM Facilities Connecting Rivers, Lakes, Estuaries and Oceans in Europe and beyond
Coverage:
Latitude: 54.183333 * Longitude: 7.900000
Date/Time Start: 2022-03-08T00:00:00 * Date/Time End: 2022-03-16T00:00:00
Minimum Elevation: -1.0 m * Maximum Elevation: -1.0 m
Event(s):
Comment:
For more information about the research site Helgoland Roads visit https://deims.org/1e96ef9b-0915-4661-849f-b3a72f5aa9b1
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Giesler, Jakob | |||
| 2 | Type of study | Study type | Giesler, Jakob | |||
| 3 | DATE/TIME | Date/Time | Giesler, Jakob | Geocode | ||
| 4 | Day of experiment | DOE | day | Giesler, Jakob | ||
| 5 | Bottle number | Bottle | Giesler, Jakob | |||
| 6 | Replicate | Repl | Giesler, Jakob | |||
| 7 | Treatment: temperature | T:temp | °C | Giesler, Jakob | ||
| 8 | Chlorophyll a | Chl a | RFU | Giesler, Jakob | Microplate reader, BioTek, Synergy H1 | measured at 395/680 nm Exc./Em. |
| 9 | Blank | Blank | Giesler, Jakob | Microplate reader, BioTek, Synergy H1 | RFU; measured at 395/680 nm Exc./Em. |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1890 data points