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Ahme, Antonia; Happe, Anika; Striebel, Maren; Olsson, Markus; Giesler, Jakob; Kline, Simon; John, Uwe (2023): Thermal performance assay of a plankton community from Helgoland Roads for an indoor mesocosm experiment  in March 2022 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.961508

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Abstract:
To choose the treatment temperatures for an indoor mesocosm temperature experiment at the ICBM in Wilhelmshaven (https://doi.pangaea.de/10.1594/PANGAEA.961155), a thermal performance curve assay was performed from the 8th of March until the 16th of March. It was started one day after filling the mesocosms with seawater from Helgoland Roads (https://deims.org/1e96ef9b-0915-4661-849f-b3a72f5aa9b1) by randomly spreading pooled sample water in 50 ml culture flasks across ten temperatures (3 °C to 30 °C in 3 °C steps) in triplicates. Their fluorescence (395/680 Excitation/Emission) was measured daily using a SYNERGY H1 microplate reader (BioTek, Winooski, Vermont, USA).
Keyword(s):
community composition; Helgoland; incubation experiment; North Sea; phytoplankton; plankton community; spring bloom; thermal performance curve
References:
Ahme, Antonia; Happe, Anika; Striebel, Maren; Olsson, Markus; Kühne, Nancy; Giesler, Jakob; Sentimenti, Alexander; Schulte-Hillen, Ruben L; Cabrerizo, Marco J; Kline, Simon; Neun, Sebastian; Naus-Wiezer, Suzanne M; Rickels, Heike; Van de Waal, Dedmer B; Torres-Valdés, Sinhué; John, Uwe (accepted): 18S-metabarcoding data of an indoor mesocosm experiment testing the effect of temperature on a plankton community from Helgoland Roads, March 2022. European Nucleotide Archive (ENA), insdc:PRJEB72441
Gall, Andrea; Uebel, Udo; Ebensen, Uwe; Hillebrand, Helmut; Meier, Sandra; Singer, Gabriel; Wacker, Alexander; Striebel, Maren (2017): Planktotrons: A novel indoor mesocosm facility for aquatic biodiversity and food web research. Limnology and Oceanography-Methods, 15(7), 663-677, https://doi.org/10.1002/lom3.10196
Related code / software:
Ahme, Antonia (2023): Data analysis R scripts for a mesocosm incubation experiment to assess the effect of temperature on a plankton community from Helgoland Roads. https://github.com/AntoniaAhme/TopTronsMesocosmIncubation
Project(s):
Funding:
Helmholtz Association of German Research Centres (HGF), grant/award no. Changing-Earth_Subtopic_6.2: Adaptation of marine life: From genes to ecosystems
Horizon 2020 (H2020), grant/award no. 871081: AQUACOSM-plus: Network of Leading Ecosystem Scale Experimental AQUAtic MesoCOSM Facilities Connecting Rivers, Lakes, Estuaries and Oceans in Europe and beyond
Coverage:
Latitude: 54.183333 * Longitude: 7.900000
Date/Time Start: 2022-03-08T00:00:00 * Date/Time End: 2022-03-16T00:00:00
Minimum Elevation: -1.0 m * Maximum Elevation: -1.0 m
Event(s):
SOT22 (HE593_SOT22)  * Latitude: 54.183333 * Longitude: 7.900000 * Date/Time: 2022-03-06T12:00:00 * Elevation: -1.0 m * DEIMS ID: deims.org * Location: North Sea * Campaign: HE593 * Basis: Heincke * Method/Device: Diaphragm pump; coupled with pipe [covered with a 200 µm net]
Comment:
For more information about the research site Helgoland Roads visit https://deims.org/1e96ef9b-0915-4661-849f-b3a72f5aa9b1
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
Event labelEventGiesler, Jakob
Type of studyStudy typeGiesler, Jakob
DATE/TIMEDate/TimeGiesler, JakobGeocode
Day of experimentDOEdayGiesler, Jakob
Bottle numberBottleGiesler, Jakob
ReplicateReplGiesler, Jakob
Treatment: temperatureT:temp°CGiesler, Jakob
Chlorophyll aChl aRFUGiesler, JakobMicroplate reader, BioTek, Synergy H1measured at 395/680 nm Exc./Em.
BlankBlankGiesler, JakobMicroplate reader, BioTek, Synergy H1RFU; measured at 395/680 nm Exc./Em.
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1890 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Event

Study type

Date/Time

DOE [day]

Bottle

Repl

T:temp [°C]

Chl a [RFU]

Blank
SOT22 Laboratory experiment2022-03-08030A13045
SOT22Laboratory experiment2022-03-08030B23020
SOT22Laboratory experiment2022-03-08030C33000
SOT22Laboratory experiment2022-03-08027A12700
SOT22Laboratory experiment2022-03-08027B227120
SOT22Laboratory experiment2022-03-08027C32760
SOT22Laboratory experiment2022-03-08024A12420
SOT22Laboratory experiment2022-03-08024B224018
SOT22Laboratory experiment2022-03-08024C32499
SOT22Laboratory experiment2022-03-08021A12100
SOT22Laboratory experiment2022-03-08021B221150
SOT22Laboratory experiment2022-03-08021C32100
SOT22Laboratory experiment2022-03-08018A11851
SOT22Laboratory experiment2022-03-08018B218143
SOT22Laboratory experiment2022-03-08018C3181611
SOT22Laboratory experiment2022-03-08015A115217
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SOT22Laboratory experiment2022-03-08012A11200
SOT22Laboratory experiment2022-03-08012B212220
SOT22Laboratory experiment2022-03-08012C312280
SOT22Laboratory experiment2022-03-0809A19027
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SOT22Laboratory experiment2022-03-0809C3905
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SOT22Laboratory experiment2022-03-0806C3604
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SOT22Laboratory experiment2022-03-0919A19627
SOT22Laboratory experiment2022-03-0919B29180
SOT22Laboratory experiment2022-03-0919C3905
SOT22Laboratory experiment2022-03-0916A16102
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SOT22Laboratory experiment2022-03-0913C331029
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SOT22Laboratory experiment2022-03-10230C33040
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SOT22Laboratory experiment2022-03-10227C32790
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SOT22Laboratory experiment2022-03-10224C32409
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SOT22Laboratory experiment2022-03-10221B221260
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SOT22Laboratory experiment2022-03-10218A118641
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SOT22Laboratory experiment2022-03-10218C3185011
SOT22Laboratory experiment2022-03-10215A1152017
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SOT22Laboratory experiment2022-03-10215C315138
SOT22Laboratory experiment2022-03-10212A112110
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SOT22Laboratory experiment2022-03-1139C39165
SOT22Laboratory experiment2022-03-1136A1612
SOT22Laboratory experiment2022-03-1136B2600
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SOT22Laboratory experiment2022-03-12421B221800
SOT22Laboratory experiment2022-03-12421C321660
SOT22Laboratory experiment2022-03-12418A118321
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SOT22Laboratory experiment2022-03-12418C3186011
SOT22Laboratory experiment2022-03-12415A1151817
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SOT22Laboratory experiment2022-03-12415C315328
SOT22Laboratory experiment2022-03-12412A112180
SOT22Laboratory experiment2022-03-12412B212420
SOT22Laboratory experiment2022-03-12412C312200
SOT22Laboratory experiment2022-03-1249A19027
SOT22Laboratory experiment2022-03-1249B29270
SOT22Laboratory experiment2022-03-1249C39215
SOT22Laboratory experiment2022-03-1246A16122
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SOT22Laboratory experiment2022-03-1243C33029
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SOT22Laboratory experiment2022-03-13527B22700
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SOT22Laboratory experiment2022-03-13524B2247918
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SOT22Laboratory experiment2022-03-13518B218743
SOT22Laboratory experiment2022-03-13518C3184611
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SOT22Laboratory experiment2022-03-1359B29590
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SOT22Laboratory experiment2022-03-14621C321850
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SOT22Laboratory experiment2022-03-14612A11200
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SOT22Laboratory experiment2022-03-14612C312430
SOT22Laboratory experiment2022-03-1469A196027
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SOT22Laboratory experiment2022-03-1466A16512
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SOT22Laboratory experiment2022-03-15730A13015
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SOT22Laboratory experiment2022-03-15730C330540
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SOT22Laboratory experiment2022-03-15727B22700
SOT22Laboratory experiment2022-03-15727C32700
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SOT22Laboratory experiment2022-03-15724B2249718
SOT22Laboratory experiment2022-03-15724C324839
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SOT22Laboratory experiment2022-03-15721B221800
SOT22Laboratory experiment2022-03-15721C3211360
SOT22Laboratory experiment2022-03-15718A118521
SOT22Laboratory experiment2022-03-15718B218623
SOT22Laboratory experiment2022-03-15718C3186811
SOT22Laboratory experiment2022-03-15715A1152917
SOT22Laboratory experiment2022-03-15715B215577
SOT22Laboratory experiment2022-03-15715C315518
SOT22Laboratory experiment2022-03-15712A11260
SOT22Laboratory experiment2022-03-15712B212100
SOT22Laboratory experiment2022-03-15712C312290
SOT22Laboratory experiment2022-03-1579A195127
SOT22Laboratory experiment2022-03-1579B29600
SOT22Laboratory experiment2022-03-1579C3955
SOT22Laboratory experiment2022-03-1576A16332
SOT22Laboratory experiment2022-03-1576B26500
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SOT22Laboratory experiment2022-03-16830A130185
SOT22Laboratory experiment2022-03-16830B23080
SOT22Laboratory experiment2022-03-16830C330560
SOT22Laboratory experiment2022-03-16827A127150
SOT22Laboratory experiment2022-03-16827B22760
SOT22Laboratory experiment2022-03-16827C32740
SOT22Laboratory experiment2022-03-16824A124970
SOT22Laboratory experiment2022-03-16824B2247518
SOT22Laboratory experiment2022-03-16824C324469
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SOT22Laboratory experiment2022-03-16818A1181341
SOT22Laboratory experiment2022-03-16818B218713
SOT22Laboratory experiment2022-03-16818C3184311
SOT22Laboratory experiment2022-03-16815A1153117
SOT22Laboratory experiment2022-03-16815B21527
SOT22Laboratory experiment2022-03-16815C315408
SOT22Laboratory experiment2022-03-16812A112260
SOT22Laboratory experiment2022-03-16812B212320
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