Ahme, Antonia; Happe, Anika; Striebel, Maren; Olsson, Markus; Kühne, Nancy; Giesler, Jakob; Sentimenti, Alexander; Schulte-Hillen, Ruben L; Cabrerizo, Marco J; Kline, Simon; Neun, Sebastian; Naus-Wiezer, Suzanne M; Rickels, Heike; Van de Waal, Dedmer B; Torres-Valdés, Sinhué; John, Uwe (2023): Temperature effects on a plankton community from Helgoland Roads tested in an indoor mesocosm experiment in March 2022 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.961155
Always quote citation above when using data! You can download the citation in several formats below.
Abstract:
To investigate the effect of temperature on a North Sea spring bloom community, we performed an incubation experiment in the mesocosm facility of the Institute for Chemistry and Biology of the Marine Environment (ICBM) in Wilhelmshaven. The plankton community was sampled from the long-term ecological research station Helgoland Roads (https://deims.org/1e96ef9b-0915-4661-849f-b3a72f5aa9b1) on the 6ᵗʰ of March, 2022. Collection of the surface community was conducted from the RV Heincke with a pipe covered with a 200 µm net that was attached to a diaphragm pump. The month-long incubation was started on the 7ᵗʰ of March in twelve indoor mesocosms, the Planktotrons (Gall et al., 2017). We chose three temperatures along the ascending part of the thermal performance curve (TPC) of the in situ community: the minimum temperature for positive growth (6°C, also the field temperature), the middle between the minimum and the optimum temperature (12 °C), and the optimum temperature for growth (18 °C). Ramping up the temperatures was conducted by 1 °C per day until the treatment temperatures were reached, resulting in a ramp phase (first twelve days) and a constant temperature phase. This dataset comprises all data collected within the experiment. Temperature, oxygen, pH, salinity, and in vivo fluorescence were measured daily at 10 am. Samples for dissolved nutrients (nitrate, nitrite, phosphate, silicate), chlorophyll a, DNA, particulate nutrients (biogenic silica, particulate organic carbon/nitrogen/phosphorus), as well as flow cytometric counts of bacteria (stained) and the unstained community were sampled every third day at the same time. The mesocosm water was generally filtered over a 200 µm mesh before sampling to exclude mesozooplankton. However, due to the appearance of large Phaeocystis colonies, additional samples without pre-filtration were taken for particulate organic carbon, nitrogen, phosphorus, and chlorophyll a starting on incubation day 15. PAR, total nitrogen and phosphorus as well as total alkalinity were measured at the start, in the middle, and at the end of the incubation. Samples for Mesozooplankton enumeration were taken and plankton species identified at the end of the experiment. All analysis scripts can be found on github (https://github.com/AntoniaAhme/TopTrons22MesocosmIncubation). The sequence data are available at the European Nucleotide Archive (ENA).
Keyword(s):
Related to:
Ahme, Antonia; Happe, Anika; Striebel, Maren; Olsson, Markus; Giesler, Jakob; Kline, Simon; John, Uwe (2023): Thermal performance assay of a plankton community from Helgoland Roads for an indoor mesocosm experiment in March 2022. PANGAEA, https://doi.org/10.1594/PANGAEA.961508
References:
Ahme, Antonia; Happe, Anika; Striebel, Maren; Olsson, Markus; Kühne, Nancy; Giesler, Jakob; Sentimenti, Alexander; Schulte-Hillen, Ruben L; Cabrerizo, Marco J; Kline, Simon; Neun, Sebastian; Naus-Wiezer, Suzanne M; Rickels, Heike; Van de Waal, Dedmer B; Torres-Valdés, Sinhué; John, Uwe (accepted): 18S-metabarcoding data of an indoor mesocosm experiment testing the effect of temperature on a plankton community from Helgoland Roads, March 2022. European Nucleotide Archive (ENA), insdc:PRJEB72441
Dickson, Andrew G; Millero, Frank J (1987): A comparison of the equilibrium constants for the dissociation of carbonic acid in seawater media. Deep Sea Research Part A. Oceanographic Research Papers, 34(10), 1733-1743, https://doi.org/10.1016/0198-0149(87)90021-5
Gall, Andrea; Uebel, Udo; Ebensen, Uwe; Hillebrand, Helmut; Meier, Sandra; Singer, Gabriel; Wacker, Alexander; Striebel, Maren (2017): Planktotrons: A novel indoor mesocosm facility for aquatic biodiversity and food web research. Limnology and Oceanography-Methods, 15(7), 663-677, https://doi.org/10.1002/lom3.10196
Grasshoff, Klaus; Kremling, Klaus; Ehrhardt, M (2009): Methods of Seawater Analysis, 3rd Completely Revised and Enlarged Edition. 632 pp
Mehrbach, C; Culberson, C H; Hawley, J E; Pytkowicx, R M (1973): Measurement of the apparent dissociation constants of carbonic acid in seawater at atmospheric pressure. Limnology and Oceanography, 18(6), 897-907, https://doi.org/10.4319/lo.1973.18.6.0897
Thrane, Jan-Erik; Kyle, Marcia; Striebel, Maren; Haande, Sigrid; Grung, Merete; Rohrlack, Thomas; Andersen, Tom; Schmitt, Francois G (2015): Spectrophotometric Analysis of Pigments: A Critical Assessment of a High-Throughput Method for Analysis of Algal Pigment Mixtures by Spectral Deconvolution. PLoS ONE, 10(9), e0137645, https://doi.org/10.1371/journal.pone.0137645
Related code / software:
Ahme, Antonia (2023): Data analysis R scripts for a mesocosm incubation experiment to assess the effect of temperature on a plankton community from Helgoland Roads. https://github.com/AntoniaAhme/TopTronsMesocosmIncubation
Pierrot, D E; Lewis, E; Wallace, D W R (2012): MS Excel Program Developed for CO2 System Calculations. Carbon Dioxide Information Analysis Center (CDIAC), https://doi.org/10.3334/CDIAC/OTG.CO2SYS_XLS_CDIAC105A
Project(s):
Changing Earth – Sustaining our Future (Helmholtz_ChangingEarth)
Funding:
Helmholtz Association of German Research Centres (HGF), grant/award no. ChangingEarth_Subtopic_6.2: Adaptation of marine life: From genes to ecosystems
Horizon 2020 (H2020), grant/award no. 871081: AQUACOSM-plus: Network of Leading Ecosystem Scale Experimental AQUAtic MesoCOSM Facilities Connecting Rivers, Lakes, Estuaries and Oceans in Europe and beyond
Coverage:
Latitude: 54.183333 * Longitude: 7.900000
Date/Time Start: 2022-03-06T12:00:00 * Date/Time End: 2022-03-06T12:00:00
Minimum Elevation: -1.0 m * Maximum Elevation: -1.0 m
Event(s):
Comment:
For more information about the research site Helgoland Roads visit https://deims.org/1e96ef9b-0915-4661-849f-b3a72f5aa9b1
Parameter(s):
# | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
---|---|---|---|---|---|---|
1 | Event label | Event | Ahme, Antonia | |||
2 | Type of study | Study type | Ahme, Antonia | |||
3 | Date/time start, experiment | Date/time start exp | Ahme, Antonia | |||
4 | Date/time end, experiment | Date/time end exp | Ahme, Antonia | |||
5 | Sample code/label | Sample label | Ahme, Antonia | |||
6 | Mesocosm label | Meso label | Ahme, Antonia | |||
7 | Treatment: temperature | T:temp | °C | Ahme, Antonia | ||
8 | Replicate | Repl | Ahme, Antonia | |||
9 | Incubation duration | Inc dur | days | Ahme, Antonia | ||
10 | Method comment | Method comm | Ahme, Antonia | whether the sample was filtered over a 200 µm mesh (yes) or not (no) before being put on a filter/fixed/measured | ||
11 | Treatment: temperature description | T:Temp descr | Ahme, Antonia | ramping/constant | ||
12 | Temperature, water | Temp | °C | Ahme, Antonia | Temperature probe, TC Mess- und Regeltechnik GmbH, PT100; measured with Temperature controller, Temperature Control GmbH, custom-built | |
13 | Radiation, photosynthetically active | PAR | µmol/m2/s | Ahme, Antonia | Conductivity and oxygen sensor, WTW, ConOx-1.5; measured with Handheld meter, WTW, Multi 3630 IDS | |
14 | Oxygen | O2 | µmol/l | Ahme, Antonia | Oxygen sensor, PreSens Precision Sensing GmbH, OXYBase WR-RS485-L5 | dissolved |
15 | pH | pH | Ahme, Antonia | Pressure resistant ORP electrode, WTW, SensoLyt ORP 900-P; measured with Handheld meter, WTW, Multi 3630 IDS | NBS scale | |
16 | Salinity | Sal | Ahme, Antonia | Conductivity probe, WTW, TetraCon 925/C; measured with Handheld meter, WTW, Multi 3630 IDS | PSU | |
17 | Fluorescence, in vivo | Fluorescence in vivo | RFU | Ahme, Antonia | Handheld Fluorometer, Turner Designs, AquaFluor | |
18 | Nitrate and Nitrite | [NO3]- + [NO2]- | µmol/l | Ahme, Antonia | Elemental analyzer, EuroVector, EA 3000 | Kept in dark at -20°C after 0.2 µm-filtration; measured colorimetrically |
19 | Silicate | Si(OH)4 | µmol/l | Ahme, Antonia | Elemental analyzer, EuroVector, EA 3000 | Kept in dark at -20°C after 0.2 µm-filtration; measured colorimetrically |
20 | Phosphate | [PO4]3- | µmol/l | Ahme, Antonia | Elemental analyzer, EuroVector, EA 3000 | Kept in dark at -20°C after 0.2 µm-filtration; measured colorimetrically |
21 | Chlorophyll a | Chl a | µg/l | Ahme, Antonia | Microplate reader, BioTek, Synergy H1 | Kept in dark at -80°C on precombusted Gf/f filter; extracted after Thrane et al. (2015); excitation wavelength: 614 nm, emission wavelength: 680 nm) |
22 | Biogenic silica | bSiO2 | µmol/l | Ahme, Antonia | Measured according to Grasshoeff et al. (2009) | |
23 | Carbon, organic, particulate | POC | µg/l | Ahme, Antonia | Carbon and hydrogen and nitrogen (CHN) analyzer, Thermo Scientific, FlashEA 1112 | Kept in dark at -20°C on precombusted Gf/f filter; after five days of drying at 60°C |
24 | Nitrogen, organic, particulate | PON | µg/l | Ahme, Antonia | Carbon and hydrogen and nitrogen (CHN) analyzer, Thermo Scientific, FlashEA 1112 | Kept in dark at -20°C on precombusted Gf/f filter; after five days of drying at 60°C |
25 | Phosphorus, organic, particulate | POP | µg/l | Ahme, Antonia | Measured according to Wetzel and Likens (2003) | Kept in dark at -20°C on precombusted Gf/f filter; quantified by molybdate reaction after digestion with a potassium peroxydisulfate solution |
26 | Bacteria | Bact | #/ml | Ahme, Antonia | Cell Sorter, BD Biosciences, BD Influx | total abundance; Kept in dark at -80°C in 1% GDA fixation; stained with SYBRgreen I (Thermofisher Scientific, Waltham, MA, USA) |
27 | Nitrogen, total dissolved | TDN | µmol/l | Ahme, Antonia | Segmented Flow Analyzer, SEAL Analytical, SEAL AA500 | Kept in dark at 4°C |
28 | Phosphorus, total dissolved | TDP | µmol/l | Ahme, Antonia | Segmented Flow Analyzer, SEAL Analytical, SEAL AA500 | Kept in dark at 4°C |
29 | Alkalinity, total | AT | µmol/kg | Ahme, Antonia | Titration analyzer, Schott Instruments, TitroLine alpha plus | Kept in dark at 4°C after 0.2 µm-filtration; corrected with certified reference (A. Dickson, Scripps Institution of Oceanography, San Diego, CA, USA) |
30 | Carbon dioxide, partial pressure | pCO2 | µatm | Ahme, Antonia | calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006) | with dissociation constants of carbonic acid by Mehrbach et al. (1973), refitted by Dickson and Millero (1987) |
31 | Bicarbonate ion | [HCO3]- | µmol/kg | Ahme, Antonia | calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006) | with dissociation constants of carbonic acid by Mehrbach et al. (1973), refitted by Dickson and Millero (1987) |
32 | Carbon trioxide | CO3 | µmol/kg | Ahme, Antonia | calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006) | with dissociation constants of carbonic acid by Mehrbach et al. (1973), refitted by Dickson and Millero (1987) |
33 | Carbon dioxide, dissolved | CO2 diss | µmol/kg | Ahme, Antonia | calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006) | with dissociation constants of carbonic acid by Mehrbach et al. (1973), refitted by Dickson and Millero (1987) |
34 | Carbon, inorganic, dissolved | DIC | µmol/kg | Ahme, Antonia | calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006) | with dissociation constants of carbonic acid by Mehrbach et al. (1973), refitted by Dickson and Millero (1987) |
35 | Mesozooplankton | Mesozooplankton | #/l | Ahme, Antonia | Stereo microscope, Leica Microsystems, S8 APO | Kept in dark at 4°C after lugole-fixation |
36 | Species | Species | Ahme, Antonia | Stereo microscope, Leica Microsystems, S8 APO | of Mesozooplankton in mesocosm; Kept in dark at 4°C after lugole-fixation; request the author for the full species list and relative proportions | |
37 | Plankton | Plank | #/ml | Ahme, Antonia | Flow cytometer, BD Biosciences, BD Accuri C6 | >20 µm size fraction; measured with fast speed for 3 minutes with a FL-3 threshold of 900; discriminated based on SSC (for sizes - size spectra calibrated with differently sized beads) |
38 | Plankton | Plank | #/ml | Ahme, Antonia | Flow cytometer, BD Biosciences, BD Accuri C6 | 1-4 µm size fraction; measured with fast speed for 3 minutes with a FL-3 threshold of 900; discriminated based on SSC (for sizes - size spectra calibrated with differently sized beads) |
39 | Phytoplankton, total | Phytopl tot | #/ml | Ahme, Antonia | Flow cytometer, BD Biosciences, BD Accuri C6 | 10-20 µm size fraction; measured with fast speed for 3 minutes with a FL-3 threshold of 900; discriminated based on high FL-3 (for chlorophyll) and SSC (for sizes - size spectra calibrated with differently sized beads) |
40 | Phytoplankton, total | Phytopl tot | #/ml | Ahme, Antonia | Flow cytometer, BD Biosciences, BD Accuri C6 | 8-12 µm size fraction; measured with fast speed for 3 minutes with a FL-3 threshold of 900; discriminated based on high FL-3 (for chlorophyll) and SSC (for sizes - size spectra calibrated with differently sized beads); Potentially Gephyrocapsa oceanica |
41 | Phytoplankton, total | Phytopl tot | #/ml | Ahme, Antonia | Flow cytometer, BD Biosciences, BD Accuri C6 | 4-10 µm size fraction; measured with fast speed for 3 minutes with a FL-3 threshold of 900; discriminated based on high FL-3 (for chlorophyll) and SSC (for sizes - size spectra calibrated with differently sized beads) |
42 | Plankton | Plank | #/ml | Ahme, Antonia | Flow cytometer, BD Biosciences, BD Accuri C6 | hetero- or mixotroph; measured with fast speed for 3 minutes with a FL-3 threshold of 900; discriminated based on low FL-3 (for chlorophyll) and SSC (for sizes - size spectra calibrated with differently sized beads) |
43 | Cyanobacteria | Cyanobact | #/ml | Ahme, Antonia | Flow cytometer, BD Biosciences, BD Accuri C6 | measured with fast speed for 3 minutes with a FL-3 threshold of 900; discriminated based on high FL-3 (for chlorophyll) and small SSC (for sizes - size spectra calibrated with differently sized beads) |
44 | Cryptophytes | Cryptophytes | #/ml | Ahme, Antonia | Flow cytometer, BD Biosciences, BD Accuri C6 | measured with fast speed for 3 minutes with a FL-3 threshold of 900; discriminated based on high FL-3 (for chlorophyll) and high SSC (for sizes - size spectra calibrated with differently sized beads) |
45 | Plankton | Plank | #/ml | Ahme, Antonia | Flow cytometer, BD Biosciences, BD Accuri C6 | containing phycocyanin; measured with fast speed for 3 minutes with a FL-3 threshold of 900; discriminated based on high FL-4 (for phycocyanin); Potentially Pseudomonas sp. |
46 | Phytoplankton, total | Phytopl tot | #/ml | Ahme, Antonia | Flow cytometer, BD Biosciences, BD Accuri C6 | 1.5-3 µm size fraction; measured with fast speed for 3 minutes with a FL-3 threshold of 900; discriminated based on high FL-3 (for chlorophyll) and SSC (for sizes - size spectra calibrated) |
47 | Phytoplankton, total | Phytopl tot | #/ml | Ahme, Antonia | Flow cytometer, BD Biosciences, BD Accuri C6 | 2-4 µm size fraction; measured with fast speed for 3 minutes with a FL-3 threshold of 900; discriminated based on high FL-3 (for chlorophyll) and SSC (for sizes - size spectra calibrated) |
48 | Phytoplankton, total | Phytopl tot | #/ml | Ahme, Antonia | Flow cytometer, BD Biosciences, BD Accuri C6 | 0.5-2 µm size fraction; measured with fast speed for 3 minutes with a FL-3 threshold of 900; discriminated based on high FL-3 (for chlorophyll) and SSC (for sizes - size spectra calibrated); Potentially Bathycoccus prasinos |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
8288 data points
Download Data
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