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Weerathunga, Veran; Huang, Wei-Jen; Dupont, Sam; Hsieh, Hsueh-Han; Piyawardhana, Nathangi; Yuan, Fei-Ling; Liao, Jhe-Syuan; Lai, Chia-Yu; Chen, Wei-Ming; Hung, Chin-Chang (2023): Seawater carbonate chemistry and fitness and immune system of Pacific White Shrimp [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.960103

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Abstract:
The atmospheric partial pressure of CO2 (pCO2) has been increasing dramatically since the beginning of the industrial revolution and about 30% of the CO2 produced by anthropogenic activities was absorbed by the ocean. This led to a perturbation of the seawater carbonate chemistry resulting in a decrease of the average surface ocean pH by 0.1 and termed ocean acidification (OA). Projections suggest that pCO2 may reach 900 μatm by the end of the twenty-first century lowering the average pH of the surface ocean by 0.4 units. The negative impacts of OA on many species of marine invertebrates such as mollusks, echinoderms, and crustaceans are well documented. However, less attention has been paid to the impacts of low pH on fitness and immune system in crustaceans. Here, we exposed Pacific white shrimps to 3 different pHs (nominal pH 8.0, 7.9, and 7.6) over a 100-days experiment. We found that, even though there were no significant effects on fitness parameters (survival, growth and allometries between length and weight), some immune markers were modified under low pH. A significant decrease in total hemocyte count and phenoloxidase activity was observed in shrimps exposed to pH 7.6 as compared to pH 8.0; and phagocytosis rate significantly decreased with decreasing pH. A significant increase in superoxide production was also observed at pH 7.6 as compared to pH 8.0. All these results suggest that a 100-days exposure to pH 7.6 did not have a direct effect on fitness but lead to a modulation of the immune response.
Keyword(s):
Animalia; Arthropoda; Benthic animals; Benthos; Containers and aquaria (20-1000 L or < 1 m**2); Growth/Morphology; Laboratory experiment; Laboratory strains; Litopenaeus vannamei; Mortality/Survival; Not applicable; Other studied parameter or process; Single species
Supplement to:
Weerathunga, Veran; Huang, Wei-Jen; Dupont, Sam; Hsieh, Hsueh-Han; Piyawardhana, Nathangi; Yuan, Fei-Ling; Liao, Jhe-Syuan; Lai, Chia-Yu; Chen, Wei-Ming; Hung, Chin-Chang (2021): Impacts of pH on the Fitness and Immune System of Pacific White Shrimp. Frontiers in Marine Science, 8, 748837, https://doi.org/10.3389/fmars.2021.748837
Documentation:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-06-28.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Type of studyStudy typeWeerathunga, Veran
2Species, unique identificationSpecies UIDWeerathunga, Veran
3Species, unique identification (URI)Species UID (URI)Weerathunga, Veran
4Species, unique identification (Semantic URI)Species UID (Semantic URI)Weerathunga, Veran
5IdentificationIDWeerathunga, Veran
6Treatment: pHT:pHWeerathunga, Veran
7pHpHWeerathunga, VeranPotentiometricNBS scale
8Carbon, inorganic, dissolvedDICµmol/kgWeerathunga, Veran
9Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmWeerathunga, VeranCalculated using CO2SYS
10Carbonate ion[CO3]2-µmol/kgWeerathunga, VeranCalculated using CO2SYS
11Bicarbonate ion[HCO3]-µmol/kgWeerathunga, VeranCalculated using CO2SYS
12SurvivalSurvival%Weerathunga, Veran
13MassMassgWeerathunga, VeranInitial
14LengthlcmWeerathunga, VeranInitial
15MassMassgWeerathunga, VeranEnd
16LengthlcmWeerathunga, VeranEnd
17Growth rateµcm/dayWeerathunga, Veran
18HemocytesHemocytes#/mlWeerathunga, Verantotal
19Superoxide productionSOPWeerathunga, Veran
20Phagocytic activityPO actWeerathunga, Veran
21PhagocytosisPhago%Weerathunga, Veranactivity
22SalinitySalWeerathunga, Veran
23Salinity, standard deviationSal std dev±Weerathunga, Veran
24Temperature, waterTemp°CWeerathunga, Veran
25Temperature, water, standard deviationTemp std dev±Weerathunga, Veran
26Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
28Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
35Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
297 data points

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