Citation:

PANGAEA.967082

Name: Seawater carbonate chemistry and the immune performance of Mercenaria mercenaria to infection by Vibrio species
Published: 2024-04-01
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Bacteria; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Entire community; Growth/Morphology; Heterotrophic prokaryotes; Immunology/Self-protection; Laboratory experiment; Mercenaria mercenaria; Mollusca; Mortality/Survival; North Atlantic; Other; Pelagos; Proteobacteria; Single species; Temperate; Vibrio sp.; Zooplankton

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Published: 2024-04-01 • DOI registered: 2024-05-01

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Abstract:

This study was designed to assess the physiological cost of resilience to acidification in the hard clam (Mercenaria mercenaria, also known as northern quahog). We examined growth and susceptibility to bacterial infection of larval and juvenile clams reared in seawater with altered pCO2. We specifically focused on changes in immune performances, measured as alterations of resistance of larvae and juveniles towards opportunistic Vibrio spp., among clams exposed to acidified seawater. By examining different life history stages, we were able to document differences in exposure time to acidification as well as potential carry-over effects. To determine if increased susceptibility to infection was direct or indirect, we examined the response of the Vibrio species used in the bacterial challenge to different pH treatments and their native presence in the treatment tanks prior to bacterial challenges. Furthermore, we determined if reduced immune function induced by exposure to acidification was permanent or if clams can rebound and restore immunity after transfer to low pCO2/high pH conditions (indicating a plastic response and/or a selection event) and if early survival in OA conditions was related to success at later stages (through either acclimation or carry over effects).

Keyword(s):

Animalia; Bacteria; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Entire community; Growth/Morphology; Heterotrophic prokaryotes; Immunology/Self-protection; Laboratory experiment; Mercenaria mercenaria; Mollusca; Mortality/Survival; North Atlantic; Other; Pelagos; Proteobacteria; Single species; Temperate; Vibrio sp.; Zooplankton

Related to:

Schwaner, Caroline; Barbosa, Michelle; Connors, Peter; Park, Tae-Jin; de Silva, Darren; Griffith, Andrew W; Gobler, Christopher J; Pales Espinosa, Emmanuelle; Allam, Bassem (2020): Experimental acidification increases susceptibility of Mercenaria mercenaria to infection by Vibrio species. Marine Environmental Research, 154, 104872, https://doi.org/10.1016/j.marenvres.2019.104872

Related code / software:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2024): seacarb: seawater carbonate chemistry with R. R package version 3.3.3. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Median Latitude: 41.154350 * Median Longitude: -72.138450 * South-bound Latitude: 40.869000 * West-bound Longitude: -73.343200 * North-bound Latitude: 41.930500 * East-bound Longitude: -70.031000

Event(s):

Hampton_Bays * Latitude: 40.869000 * Longitude: -72.517600 * Method/Device: Experiment (EXP)

Northport * Latitude: 40.900900 * Longitude: -73.343200 * Method/Device: Experiment (EXP)

Riverhead_OA * Latitude: 40.917000 * Longitude: -72.662000 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2024) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2024-03-28.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Allam, Bassem
2	Species, unique identification	Species UID		Allam, Bassem
3	Species, unique identification (URI)	Species UID (URI)		Allam, Bassem
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Allam, Bassem
5	Treatment	Treat		Allam, Bassem
6	Time in days	Time d	days	Allam, Bassem		post-fertilization
7	Length	l	µm	Allam, Bassem		Larval
8	Duration	Duration	months	Allam, Bassem		post fertilization
9	Width	w	mm	Allam, Bassem		juvenile
10	Length	l	mm	Allam, Bassem		juvenile
11	Bacteria, abundance in colony forming units	Bacteria CFU	CFU/g	Allam, Bassem		Vibrio in tissue
12	Bacterial abundance, standard deviation	Bact std dev	±	Allam, Bassem		Vibrio in tissue
13	Bacteria, abundance in colony forming units	Bacteria CFU	CFU/g	Allam, Bassem		Marine heterotrophic bacteria in tissues
14	Bacterial abundance, standard deviation	Bact std dev	±	Allam, Bassem		Marine heterotrophic bacteria in tissues
15	Bacteria, abundance in colony forming units	Bacteria CFU	CFU/ml	Allam, Bassem		Vibrio in seawater
16	Bacterial abundance, standard deviation	Bact std dev	±	Allam, Bassem		Vibrio in seawater
17	Bacteria, abundance in colony forming units	Bacteria CFU	CFU/ml	Allam, Bassem		Marine heterotrophic bacteria in seawater
18	Bacterial abundance, standard deviation	Bact std dev	±	Allam, Bassem		Marine heterotrophic bacteria in seawater
19	Time in hours	Time h	h	Allam, Bassem
20	Bacteria, abundance in colony forming units	Bacteria CFU	CFU/ml	Allam, Bassem		Vibrio species growth
21	Bacterial abundance, standard deviation	Bact std dev	±	Allam, Bassem		Vibrio species growth
22	Individuals	Ind	#	Allam, Bassem		Live
23	Individuals	Ind	#	Allam, Bassem		Dead
24	Temperature, water	Temp	°C	Allam, Bassem
25	Temperature, water, standard deviation	Temp std dev	±	Allam, Bassem
26	pH, total scale	pHT		Allam, Bassem		total scale
27	pH, standard deviation	pH std dev	±	Allam, Bassem		total scale
28	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Allam, Bassem	Calculated with seacarb package in R
29	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Allam, Bassem	Calculated with seacarb package in R
30	Calcite saturation state	Omega Cal		Allam, Bassem	Calculated with seacarb package in R
31	Calcite saturation state, standard deviation	Omega Cal std dev	±	Allam, Bassem	Calculated with seacarb package in R
32	Aragonite saturation state	Omega Arg		Allam, Bassem	Calculated with seacarb package in R
33	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Allam, Bassem	Calculated with seacarb package in R
34	Carbon, inorganic, dissolved	DIC	µmol/kg	Allam, Bassem
35	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Allam, Bassem
36	Carbonate ion	[CO3]2-	µmol/kg	Allam, Bassem	Calculated with seacarb package in R
37	Carbonate ion, standard deviation	[CO3]2- std dev	±	Allam, Bassem	Calculated with seacarb package in R
38	Alkalinity, total	AT	µmol/kg	Allam, Bassem	Calculated with seacarb package in R
39	Alkalinity, total, standard deviation	AT std dev	±	Allam, Bassem	Calculated with seacarb package in R
40	Salinity	Sal		Allam, Bassem
41	Salinity, standard deviation	Sal std dev	±	Allam, Bassem
42	Temperature, water	Temp	°C	Allam, Bassem		after transplant
43	Temperature, water, standard deviation	Temp std dev	±	Allam, Bassem		after transplant
44	pH, total scale	pHT		Allam, Bassem		total scale, after transplant
45	pH, standard deviation	pH std dev	±	Allam, Bassem		total scale, after transplant
46	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Allam, Bassem	Calculated with seacarb package in R	after transplant
47	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Allam, Bassem	Calculated with seacarb package in R	after transplant
48	Calcite saturation state	Omega Cal		Allam, Bassem	Calculated with seacarb package in R	after transplant
49	Calcite saturation state, standard deviation	Omega Cal std dev	±	Allam, Bassem	Calculated with seacarb package in R	after transplant
50	Aragonite saturation state	Omega Arg		Allam, Bassem	Calculated with seacarb package in R	after transplant
51	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Allam, Bassem	Calculated with seacarb package in R	after transplant
52	Carbon, inorganic, dissolved	DIC	µmol/kg	Allam, Bassem		after transplant
53	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Allam, Bassem		after transplant
54	Carbonate ion	[CO3]2-	µmol/kg	Allam, Bassem	Calculated with seacarb package in R	after transplant
55	Carbonate ion, standard deviation	[CO3]2- std dev	±	Allam, Bassem	Calculated with seacarb package in R	after transplant
56	Alkalinity, total	AT	µmol/kg	Allam, Bassem	Calculated with seacarb package in R	after transplant
57	Alkalinity, total, standard deviation	AT std dev	±	Allam, Bassem	Calculated with seacarb package in R	after transplant
58	Salinity	Sal		Allam, Bassem		after transplant
59	Salinity, standard deviation	Sal std dev	±	Allam, Bassem		after transplant
60	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
61	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
62	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
63	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
64	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
65	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
66	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
67	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
68	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
69	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
70	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
71	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
72	Alkalinity, total, standard deviation	AT std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
73	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
74	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
75	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
76	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
77	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transplant
78	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	after transplant
79	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transplant
80	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	after transplant
81	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transplant
82	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	after transplant
83	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transplant
84	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	after transplant
85	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transplant
86	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	after transplant
87	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transplant
88	Alkalinity, total, standard deviation	AT std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	after transplant
89	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transplant
90	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	after transplant
91	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transplant
92	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	after transplant

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1334 data points

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