Rokitta, Sebastian D; Großmann, Christian H; Werner, Elisa; Moye, Jannika; Castellani, Giulia; Nöthig, Eva-Maria; Rost, Björn (2022): Isochrysis sp.'s responses to temperature (2 vs. 6 °C), light (55 vs. 160 µmol photons m-2 s-1) and pCO2 (400 vs. 1000 µatm) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.948489
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Published: 2022-09-19 • DOI registered: 2023-12-21
Abstract:
We assessed the responses of solitary cells of Arctic Isochrysis sp. (Strain PS78) grown under a matrix of temperature (2°C vs. 6°C), light intensity (55 vs. 160 μmol photons m-2 s-1) and CO2 partial pressures (pCO2; 400 vs. 1000 μatm). Before the experiments, the strain (isolated during Polarstern cruise PS78 in 2011) was kept as stock culture at 1° in 0.2 µm sterile-filtered Arctic seawater (Salinity 33), enriched with vitamins and trace metals according to F/2 medium (Guillard & Ryther, 1962). Nitrate and phosphate were added in concentrations of 100 and 6 µmol L-1, respectively. Experiments were conducted between May 2016 and September 2017 at the Alfred-Wegener-Institute, using standardized media and continuous light exposition. Next to acclimation parameters (growth rates, particulate and dissolved organic carbon and nitrogen, chlorophyll a content), we measured physiological processes in-vivo (electron transport rates and net photosynthesis) using fast-repetition rate fluorometry and membrane-inlet mass spectrometry.
Keyword(s):
Supplement to:
Rokitta, Sebastian D; Großmann, Christian H; Werner, Elisa; Moye, Jannika; Castellani, Giulia; Nöthig, Eva-Maria; Rost, Björn (2023): Future warming stimulates growth and photosynthesis in an Arctic microalga more strongly than changes in light intensity or pCO2. Limnology and Oceanography, 68(12), 2789-2799, https://doi.org/10.1002/lno.12460
Related to:
Guillard, R R L; Ryther, J H (1962): Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (cleve) Gran. Canadian Journal of Microbiology, 8(2), 229-239, https://doi.org/10.1139/m62-029
Knap, Anthony H; Michaels, A; Close, A R; Ducklow, Hugh W; Dickson, Andrew G (1996): Protocols for the Joint Global Ocean Flux Study (JGOFS) Core Measurements. JGOFS, Reprint of the IOC Manuals and Guides No. 29, UNESCO 1994, 19, 210 pp, hdl:10013/epic.27912.d001
Kottmeier, Dorothee; Rokitta, Sebastian D; Rost, Björn (2016): Acidification, not carbonation, is the major regulator of carbon fluxes in the coccolithophore Emiliania huxleyi. New Phytologist, 211(1), 126-137, https://doi.org/10.1111/nph.13885
Oxborough, Kevin; Moore, C Mark; Suggett, David J; Lawson, Tracy; Chan, Hoi Ga; Geider, Richard J (2012): Direct estimation of functional PSII reaction center concentration and PSII electron flux on a volume basis: a new approach to the analysis of Fast Repetition Rate fluorometry (FRRf) data. Limnology and Oceanography-Methods, 10(3), 142-154, https://doi.org/10.4319/lom.2012.10.142
Pierrot, D E; Lewis, E; Wallace, D W R (2006): MS Excel Program Developed for CO2 System Calculations. Carbon Dioxide Information Analysis Center, Oak Ridge National Laboratory, U.S. Department of Energy, https://doi.org/10.3334/CDIAC/otg.CO2SYS_XLS_CDIAC105a
Rokitta, Sebastian D; Rost, Björn (2012): Effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi. Limnology and Oceanography, 57(2), 607-618, https://doi.org/10.4319/lo.2012.57.2.0607
Stoll, Michel H C; Bakker, Karel; Nobbe, G; Haese, Ralf R (2001): Continuous-Flow Analysis of Dissolved Inorganic Carbon Content in Seawater. Analytical Chemistry, 73(17), 4111-4116, https://doi.org/10.1021/ac010303r
Coverage:
Latitude: 78.835000 * Longitude: 5.994000
Date/Time Start: 2016-05-10T00:00:00 * Date/Time End: 2017-09-15T00:00:00
Event(s):
Isochrysis_sp_PS78 * Latitude: 78.835000 * Longitude: 5.994000 * Location: Fram Strait * Method/Device: Experiment (EXP) * Comment: Strain isolated from the Fram strait
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experimental treatment | Exp treat | Rokitta, Sebastian D | |||
| 2 | Type of study | Study type | Rokitta, Sebastian D | Bottle incubation | ||
| 3 | DATE/TIME | Date/Time | Rokitta, Sebastian D | Geocode – Start of experiment | ||
| 4 | DATE/TIME | Date/Time | Rokitta, Sebastian D | Geocode – End of experiment | ||
| 5 | Species, unique identification | Species UID | Rokitta, Sebastian D | |||
| 6 | Species, unique identification (URI) | Species UID (URI) | Rokitta, Sebastian D | |||
| 7 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Rokitta, Sebastian D | |||
| 8 | Strain | Strain | Rokitta, Sebastian D | |||
| 9 | Identification | ID | Rokitta, Sebastian D | Replicate identifier | ||
| 10 | Treatment: temperature | T:temp | °C | Rokitta, Sebastian D | Thermometer, internal, Aquatrode Plus, Metrohm | |
| 11 | Treatment: light intensity | T:Io | µmol/m2/s | Rokitta, Sebastian D | Universal light meter & data logger, WALZ, ULM-500, with 4Pi sensor, LI-COR | |
| 12 | Treatment: partial pressure of carbon dioxide | T:pCO2 | µatm | Rokitta, Sebastian D | pCO2 mixed from CO2-free air and pure CO2 with a custom built gas mixing system | |
| 13 | Carbon, inorganic, dissolved | DIC | µmol/kg | Rokitta, Sebastian D | Colorimetric detection, TRAACs continuous flow autoanalyzer, according to the method of Stoll et al. (2001) | initial |
| 14 | pH, NBS scale | pH NBS | Rokitta, Sebastian D | pH 826 mobile handheld device, with Aquatrode Plus, Metrohm | initial, NBS scale | |
| 15 | Temperature, water | Temp | °C | Rokitta, Sebastian D | Thermometer, internal, Aquatrode Plus, Metrohm | during initial pH measurement |
| 16 | Alkalinity, total | AT | µmol/kg | Rokitta, Sebastian D | calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006) | initial |
| 17 | Carbon dioxide, partial pressure | pCO2 | µatm | Rokitta, Sebastian D | calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006) | initial |
| 18 | Carbon, inorganic, dissolved | DIC | µmol/kg | Rokitta, Sebastian D | Colorimetric detection, TRAACs continuous flow autoanalyzer, according to the method of Stoll et al. (2001) | final |
| 19 | pH, NBS scale | pH NBS | Rokitta, Sebastian D | pH 826 mobile handheld device, with Aquatrode Plus, Metrohm | final, NBS scale | |
| 20 | Temperature, water | Temp | °C | Rokitta, Sebastian D | Thermometer, internal, Aquatrode Plus, Metrohm | during final pH measurement |
| 21 | Alkalinity, total | AT | µmol/kg | Rokitta, Sebastian D | calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006) | final |
| 22 | Isochrysis sp., growth rate | Isochrysis sp. µ | 1/day | Rokitta, Sebastian D | Coulter counter, Beckman Coulter, Multisizer 3 | specific growth rate, derived from daily counts |
| 23 | Isochrysis sp., particulate organic carbon quota per cell | Isochrysis sp. POC quota/cell | pg/# | Rokitta, Sebastian D | Elemental analyzer, EuroVector, EuroEA | |
| 24 | Isochrysis sp., particulate organic nitrogen quota per cell | Isochrysis sp. PON quota/cell | pg/# | Rokitta, Sebastian D | Elemental analyzer, EuroVector, EuroEA | |
| 25 | Isochrysis sp., particulate organic carbon production per cell | Isochrysis sp. POC prod/cell | pg/#/day | Rokitta, Sebastian D | calculated from growth rate and particulate organic carbon (POC) quota | |
| 26 | Isochrysis sp., particulate organic nitrogen production per cell | Isochrysis sp. PON prod/cell | pg/#/day | Rokitta, Sebastian D | calculated from growth rate and particulate organic nitrogen (PON) quota | |
| 27 | Isochrysis sp., carbon, organic, particulate/nitrogen, organic, particulate ratio | Isochrysis sp. POC/PON | mol/mol | Rokitta, Sebastian D | calculated from particulate organic carbon (POC) and particulate organic nitrogen (PON) quota | |
| 28 | Isochrysis sp., chlorophyll a quota per cell | Isochrysis sp. chl a quota/cell | pg/# | Rokitta, Sebastian D | Fluorometer, Turner Designs, TD-700, using acidification method (Knap et al., 1996) | |
| 29 | Isochrysis sp., chlorophyll a/carbon, organic, particulate ratio | Isochrysis sp. chl a/POC | pg/pg | Rokitta, Sebastian D | calculated from chlorophyll a (chl a) and particulate organic carbon (POC) quota | |
| 30 | Electron transport rate, relative | rETR | Rokitta, Sebastian D | Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012); raw data obtained using a fast-repetition rate fluoremeter (FRRF), FastOcean PTX with FastAct Laboratory system, Chelsea Technologies after Oxborough et al. (201 | FRRF-Vmax [relative electron transfer rate] | |
| 31 | Light acclimation index | lk | µmol photons/m2/s | Rokitta, Sebastian D | Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012); raw data obtained using a fast-repetition rate fluoremeter (FRRF), FastOcean PTX with FastAct Laboratory system, Chelsea Technologies after Oxborough et al. (201 | FRRF-Ik [µmol photons m-2 s-1] |
| 32 | Initial slope of the photosynthesis-irradiance curve, relative electron transfer rate per unit light | alpha | 1/(µmol photons/m2/s) | Rokitta, Sebastian D | Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012); raw data obtained using a fast-repetition rate fluoremeter (FRRF), FastOcean PTX with FastAct Laboratory system, Chelsea Technologies after Oxborough et al. (201 | FRRF-alpha [relative electron transfer rate * (µmol photons m-2 s-1)-1] |
| 33 | Maximum photosynthesis rate, oxygen, per chlorophyll a | Pmax O2/Chl a | µmol/mg/h | Rokitta, Sebastian D | Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012), raw data obtained using a membrane-inlet mass spectrometer (MIMS) as described in Kottmeier, Rokitta & Rost (2016) | MIMS-Vmax [µmol O2 h-1 (mg Chl a)-1] |
| 34 | Light acclimation index | lk | µmol photons/m2/s | Rokitta, Sebastian D | Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012), raw data obtained using a membrane-inlet mass spectrometer (MIMS) as described in Kottmeier, Rokitta & Rost (2016) | MIMS-Ik [µmol photons m-2 s-1] |
| 35 | Initial slope of the photosynthesis-irradiance curve | alpha | (µmol O2/mg Chl a/h)/(µmol photons/m2/s) | Rokitta, Sebastian D | Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012), raw data obtained using a membrane-inlet mass spectrometer (MIMS) as described in Kottmeier, Rokitta & Rost (2016) | MIMS-alpha [(µmol O2 h-1 (mg Chl a)-1) (µmol photons m-2 s-1)-1] |
Change history:
2025-05-12T13:38:46 – The species name Phaeocystis pouchetii was replaced by Isochrysis sp. in the dataset title, keywords, abstract, event and data table. (Reason: new species identification of PS78 algal culture with sequencing)
2025-05-13T09:31:43 – Parameters 22-29: The species name in the parameter names were updated due to new species identification of the algal culture (see above).
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
908 data points