Rokitta, Sebastian D; Großmann, Christian H; Werner, Elisa; Moye, Jannika; Castellani, Giulia; Nöthig, Eva-Maria; Rost, Björn (2024): Seawater carbonate chemistry and Phaeocystis pouchetii's responses to temperature (2 vs. 6 °C), light (55 vs. 160 µmol photons m-2 s-1) and pCO2 (400 vs. 1000 µatm) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.964944
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Abstract:
We assessed the responses of solitary cells of Arctic Phaeocystis pouchetii (Strain PS78) grown under a matrix of temperature (2°C vs. 6°C), light intensity (55 vs. 160 μmol photons m-2 s-1) and CO2 partial pressures (pCO2; 400 vs. 1000 μatm). Before the experiments, the strain (isolated during Polarstern cruise PS78 in 2011) was kept as stock culture at 1° in 0.2 µm sterile-filtered Arctic seawater (Salinity 33), enriched with vitamins and trace metals according to F/2 medium (Guillard & Ryther, 1962). Nitrate and phosphate were added in concentrations of 100 and 6 µmol L-1, respectively. Experiments were conducted between May 2016 and September 2017 at the Alfred-Wegener-Institute, using standardized media and continuous light exposition. Next to acclimation parameters (growth rates, particulate and dissolved organic carbon and nitrogen, chlorophyll a content), we measured physiological processes in-vivo (electron transport rates and net photosynthesis) using fast-repetition rate fluorometry and membrane-inlet mass spectrometry. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2024-01-20.
Keyword(s):
Supplement to:
Rokitta, Sebastian D; Großmann, Christian H; Werner, Elisa; Moye, Jannika; Castellani, Giulia; Nöthig, Eva-Maria; Rost, Björn (2023): Future warming stimulates growth and photosynthesis in an Arctic microalga more strongly than changes in light intensity or pCO2. Limnology and Oceanography, 68(12), 2789-2799, https://doi.org/10.1002/lno.12460
Source:
Rokitta, Sebastian D; Großmann, Christian H; Werner, Elisa; Moye, Jannika; Castellani, Giulia; Nöthig, Eva-Maria; Rost, Björn (2022): Phaeocystis pouchetii's responses to temperature (2 vs. 6 °C), light (55 vs. 160 µmol photons m-2 s-1) and pCO2 (400 vs. 1000 µatm) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.948489
Documentation:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html
Project(s):
Coverage:
Latitude: 78.835000 * Longitude: 5.994000
Event(s):
Phaeocystis_pouchetii_PS78 * Latitude: 78.835000 * Longitude: 5.994000 * Location: Fram Strait * Method/Device: Experiment (EXP) * Comment: Strain isolated from the Fram strait
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Rokitta, Sebastian D | Bottle incubation | ||
| 2 | Species, unique identification | Species UID | Rokitta, Sebastian D | |||
| 3 | Species, unique identification (URI) | Species UID (URI) | Rokitta, Sebastian D | |||
| 4 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Rokitta, Sebastian D | |||
| 5 | Experimental treatment | Exp treat | Rokitta, Sebastian D | |||
| 6 | Date/time start | Date/time start | Rokitta, Sebastian D | Start of experiment | ||
| 7 | Date/time end | Date/time end | Rokitta, Sebastian D | End of experiment | ||
| 8 | Strain | Strain | Rokitta, Sebastian D | |||
| 9 | Replicate | Repl | Rokitta, Sebastian D | |||
| 10 | Treatment: temperature | T:temp | °C | Rokitta, Sebastian D | Thermometer, internal, Aquatrode Plus, Metrohm | |
| 11 | Treatment: light intensity | T:Io | µmol/m2/s | Rokitta, Sebastian D | Universal light meter & data logger, WALZ, ULM-500, with 4Pi sensor, LI-COR | |
| 12 | Treatment: partial pressure of carbon dioxide | T:pCO2 | µatm | Rokitta, Sebastian D | pCO2 mixed from CO2-free air and pure CO2 with a custom built gas mixing system | |
| 13 | Carbon, inorganic, dissolved | DIC | µmol/kg | Rokitta, Sebastian D | Colorimetric detection, TRAACs continuous flow autoanalyzer, according to the method of Stoll et al. (2001) | initial |
| 14 | pH | pH | Rokitta, Sebastian D | pH 826 mobile handheld device, with Aquatrode Plus, Metrohm | initial, NBS scale | |
| 15 | Temperature, water | Temp | °C | Rokitta, Sebastian D | Thermometer, internal, Aquatrode Plus, Metrohm | during initial pH measurement |
| 16 | Alkalinity, total | AT | µmol/kg | Rokitta, Sebastian D | calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006) | initial |
| 17 | Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) | pCO2water_SST_wet | µatm | Rokitta, Sebastian D | calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006) | initial |
| 18 | Carbon, inorganic, dissolved | DIC | µmol/kg | Rokitta, Sebastian D | Colorimetric detection, TRAACs continuous flow autoanalyzer, according to the method of Stoll et al. (2001) | final |
| 19 | pH | pH | Rokitta, Sebastian D | pH 826 mobile handheld device, with Aquatrode Plus, Metrohm | final, NBS scale | |
| 20 | Temperature, water | Temp | °C | Rokitta, Sebastian D | Thermometer, internal, Aquatrode Plus, Metrohm | during final pH measurement |
| 21 | Alkalinity, total | AT | µmol/kg | Rokitta, Sebastian D | calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006) | final |
| 22 | Growth rate | µ | 1/day | Rokitta, Sebastian D | Coulter counter, Beckman Coulter, Multisizer 3 | specific growth rate, derived from daily counts |
| 23 | Carbon, organic, particulate, per cell | POC/cell | pg/# | Rokitta, Sebastian D | Elemental analyzer, EuroVector, EuroEA | |
| 24 | Nitrogen, organic, particulate, per cell | PON/cell | pg/# | Rokitta, Sebastian D | Elemental analyzer, EuroVector, EuroEA | |
| 25 | Carbon, organic, particulate, production per cell | POC prod/cell | pg/#/day | Rokitta, Sebastian D | calculated from growth rate and particulate organic carbon (POC) quota | |
| 26 | Production of particulate organic nitrogen | PON prod | pg/#/day | Rokitta, Sebastian D | calculated from growth rate and particulate organic nitrogen (PON) quota | |
| 27 | Carbon, organic, particulate/Nitrogen, organic, particulate ratio | POC/PON | Rokitta, Sebastian D | calculated from particulate organic carbon (POC) and particulate organic nitrogen (PON) quota | ||
| 28 | Chlorophyll a per cell | Chl a/cell | pg/# | Rokitta, Sebastian D | Fluorometer, Turner Designs, TD-700, using acidification method (Knap et al., 1996) | |
| 29 | Chlorophyll a/particulate organic carbon ratio | Chl a/POC | Rokitta, Sebastian D | calculated from chlorophyll a (chl a) and particulate organic carbon (POC) quota | ||
| 30 | Electron transport rate, relative | rETR | Rokitta, Sebastian D | Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012); raw data obtained using a fast-repetition rate fluoremeter (FRRF), FastOcean PTX with FastAct Laboratory system, Chelsea Technologies after Oxborough et al. (201 | FRRF-Vmax [relative electron transfer rate] | |
| 31 | Light acclimation index | lk | µmol photons/m2/s | Rokitta, Sebastian D | Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012); raw data obtained using a fast-repetition rate fluoremeter (FRRF), FastOcean PTX with FastAct Laboratory system, Chelsea Technologies after Oxborough et al. (201 | FRRF-Ik [?mol photons m-2 s-1] |
| 32 | Initial slope of the photosynthesis-irradiance curve, relative electron transfer rate per unit light | alpha | 1/(µmol photons/m2/s) | Rokitta, Sebastian D | Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012); raw data obtained using a fast-repetition rate fluoremeter (FRRF), FastOcean PTX with FastAct Laboratory system, Chelsea Technologies after Oxborough et al. (201 | FRRF-alpha [relative electron transfer rate * (?mol photons m-2 s-1)-1] |
| 33 | Maximum photosynthesis rate, oxygen, per chlorophyll a | Pmax O2/Chl a | µmol/mg/h | Rokitta, Sebastian D | Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012), raw data obtained using a membrane-inlet mass spectrometer (MIMS) as described in Kottmeier, Rokitta & Rost (2016) | MIMS-Vmax [?mol O2 h-1 (mg Chl a)-1] |
| 34 | Light acclimation index | lk | µmol photons/m2/s | Rokitta, Sebastian D | Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012), raw data obtained using a membrane-inlet mass spectrometer (MIMS) as described in Kottmeier, Rokitta & Rost (2016) | MIMS-Ik [?mol photons m-2 s-1] |
| 35 | Initial slope of the photosynthesis-irradiance curve, relative electron transfer rate per unit light | alpha | 1/(µmol photons/m2/s) | Rokitta, Sebastian D | Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012), raw data obtained using a membrane-inlet mass spectrometer (MIMS) as described in Kottmeier, Rokitta & Rost (2016) | MIMS-alpha [(?mol O2 h-1 (mg Chl a)-1) (?mol photons m-2 s-1)-1] |
| 36 | Carbonate system computation flag | CSC flag | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | ||
| 37 | pH | pH | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | initial, total scale | |
| 38 | Carbon dioxide | CO2 | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | initial |
| 39 | Fugacity of carbon dioxide (water) at sea surface temperature (wet air) | fCO2water_SST_wet | µatm | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | initial |
| 40 | Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) | pCO2water_SST_wet | µatm | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | initial |
| 41 | Bicarbonate ion | [HCO3]- | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | initial |
| 42 | Carbonate ion | [CO3]2- | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | initial |
| 43 | Alkalinity, total | AT | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | initial |
| 44 | Aragonite saturation state | Omega Arg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | initial | |
| 45 | Calcite saturation state | Omega Cal | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | initial | |
| 46 | pH | pH | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | final, total scale | |
| 47 | Carbon dioxide | CO2 | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | final |
| 48 | Fugacity of carbon dioxide (water) at sea surface temperature (wet air) | fCO2water_SST_wet | µatm | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | final |
| 49 | Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) | pCO2water_SST_wet | µatm | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | final |
| 50 | Bicarbonate ion | [HCO3]- | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | final |
| 51 | Carbonate ion | [CO3]2- | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | final |
| 52 | Alkalinity, total | AT | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | final |
| 53 | Aragonite saturation state | Omega Arg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | final | |
| 54 | Calcite saturation state | Omega Cal | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | final |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1580 data points