Citation:

PANGAEA.964944

Name: Seawater carbonate chemistry and Isochrysis sp.'s responses to temperature (2 vs. 6 °C), light (55 vs. 160 µmol photons m-2 s-1) and pCO2 (400 vs. 1000 µatm)
Published: 2024-01-20
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Arctic; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Haptophyta; Isochrysis sp.; Laboratory experiment; Light; Ocean acidification; Open ocean; Pelagos; Phytoplankton; Polar; Primary production/Photosynthesis; Single species; Temperature

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Published: 2024-01-20 • DOI registered: 2024-02-19

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Abstract:

We assessed the responses of solitary cells of Arctic Isochrysis sp. grown under a matrix of temperature (2°C vs. 6°C), light intensity (55 vs. 160 μmol photons m-2 s-1) and CO2 partial pressures (pCO2; 400 vs. 1000 μatm). Before the experiments, the strain (isolated during Polarstern cruise PS78 in 2011) was kept as stock culture at 1° in 0.2 µm sterile-filtered Arctic seawater (Salinity 33), enriched with vitamins and trace metals according to F/2 medium (Guillard & Ryther, 1962). Nitrate and phosphate were added in concentrations of 100 and 6 µmol L-1, respectively. Experiments were conducted between May 2016 and September 2017 at the Alfred-Wegener-Institute, using standardized media and continuous light exposition. Next to acclimation parameters (growth rates, particulate and dissolved organic carbon and nitrogen, chlorophyll a content), we measured physiological processes in-vivo (electron transport rates and net photosynthesis) using fast-repetition rate fluorometry and membrane-inlet mass spectrometry. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2024-01-20.

Keyword(s):

Arctic; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Haptophyta; Isochrysis sp.; Laboratory experiment; Light; Ocean acidification; Open ocean; Pelagos; Phytoplankton; Polar; Primary production/Photosynthesis; Single species; Temperature

Supplement to:

Rokitta, Sebastian D; Großmann, Christian H; Werner, Elisa; Moye, Jannika; Castellani, Giulia; Nöthig, Eva-Maria; Rost, Björn (2023): Future warming stimulates growth and photosynthesis in an Arctic microalga more strongly than changes in light intensity or pCO2. Limnology and Oceanography, 68(12), 2789-2799, https://doi.org/10.1002/lno.12460

Source:

Rokitta, Sebastian D; Großmann, Christian H; Werner, Elisa; Moye, Jannika; Castellani, Giulia; Nöthig, Eva-Maria; Rost, Björn (2022): Isochrysis sp.'s responses to temperature (2 vs. 6 °C), light (55 vs. 160 µmol photons m-2 s-1) and pCO2 (400 vs. 1000 µatm) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.948489

Documentation:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 78.835000 * Longitude: 5.994000

Event(s):

Isochrysis_sp_PS78 * Latitude: 78.835000 * Longitude: 5.994000 * Location: Fram Strait * Method/Device: Experiment (EXP) * Comment: Strain isolated from the Fram strait

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Rokitta, Sebastian D
2	Species	Species		Rokitta, Sebastian D
3	Experimental treatment	Exp treat		Rokitta, Sebastian D
4	Date/time start	Date/time start		Rokitta, Sebastian D		Start of experiment
5	Date/time end	Date/time end		Rokitta, Sebastian D		End of experiment
6	Strain	Strain		Rokitta, Sebastian D
7	Replicate	Repl		Rokitta, Sebastian D
8	Treatment: temperature	T:temp	°C	Rokitta, Sebastian D	Thermometer, internal, Aquatrode Plus, Metrohm
9	Treatment: light intensity	T:Io	µmol/m²/s	Rokitta, Sebastian D	Universal light meter & data logger, WALZ, ULM-500, with 4Pi sensor, LI-COR
10	Treatment: partial pressure of carbon dioxide	T:pCO2	µatm	Rokitta, Sebastian D	pCO2 mixed from CO2-free air and pure CO2 with a custom built gas mixing system
11	Carbon, inorganic, dissolved	DIC	µmol/kg	Rokitta, Sebastian D	Colorimetric detection, TRAACs continuous flow autoanalyzer, according to the method of Stoll et al. (2001)	initial
12	pH, NBS scale	pH NBS		Rokitta, Sebastian D	pH 826 mobile handheld device, with Aquatrode Plus, Metrohm	initial, NBS scale
13	Temperature, water	Temp	°C	Rokitta, Sebastian D	Thermometer, internal, Aquatrode Plus, Metrohm	during initial pH measurement
14	Alkalinity, total	AT	µmol/kg	Rokitta, Sebastian D	calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006)	initial
15	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Rokitta, Sebastian D	calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006)	initial
16	Carbon, inorganic, dissolved	DIC	µmol/kg	Rokitta, Sebastian D	Colorimetric detection, TRAACs continuous flow autoanalyzer, according to the method of Stoll et al. (2001)	final
17	pH, NBS scale	pH NBS		Rokitta, Sebastian D	pH 826 mobile handheld device, with Aquatrode Plus, Metrohm	final, NBS scale
18	Temperature, water	Temp	°C	Rokitta, Sebastian D	Thermometer, internal, Aquatrode Plus, Metrohm	during final pH measurement
19	Alkalinity, total	AT	µmol/kg	Rokitta, Sebastian D	calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006)	final
20	Growth rate	µ	1/day	Rokitta, Sebastian D	Coulter counter, Beckman Coulter, Multisizer 3	specific growth rate, derived from daily counts
21	Carbon, organic, particulate, per cell	POC/cell	pg/#	Rokitta, Sebastian D	Elemental analyzer, EuroVector, EuroEA
22	Nitrogen, organic, particulate, per cell	PON/cell	pg/#	Rokitta, Sebastian D	Elemental analyzer, EuroVector, EuroEA
23	Carbon, organic, particulate, production per cell	POC prod/cell	pg/#/day	Rokitta, Sebastian D	calculated from growth rate and particulate organic carbon (POC) quota
24	Nitrogen, organic, particulate, production	PON prod	pg/#/day	Rokitta, Sebastian D	calculated from growth rate and particulate organic nitrogen (PON) quota
25	Carbon, organic, particulate/Nitrogen, organic, particulate ratio	POC/PON		Rokitta, Sebastian D	calculated from particulate organic carbon (POC) and particulate organic nitrogen (PON) quota
26	Chlorophyll a per cell	Chl a/cell	pg/#	Rokitta, Sebastian D	Fluorometer, Turner Designs, TD-700, using acidification method (Knap et al., 1996)
27	Chlorophyll a/particulate organic carbon ratio	Chl a/POC		Rokitta, Sebastian D	calculated from chlorophyll a (chl a) and particulate organic carbon (POC) quota
28	Electron transport rate, relative	rETR		Rokitta, Sebastian D	Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012); raw data obtained using a fast-repetition rate fluoremeter (FRRF), FastOcean PTX with FastAct Laboratory system, Chelsea Technologies after Oxborough et al. (201	FRRF-Vmax [relative electron transfer rate]
29	Light acclimation index	lk	µmol photons/m²/s	Rokitta, Sebastian D	Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012); raw data obtained using a fast-repetition rate fluoremeter (FRRF), FastOcean PTX with FastAct Laboratory system, Chelsea Technologies after Oxborough et al. (201	FRRF-Ik [µmol photons m-2 s-1]
30	Initial slope of the photosynthesis-irradiance curve, relative electron transfer rate per unit light	alpha	1/(µmol photons/m²/s)	Rokitta, Sebastian D	Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012); raw data obtained using a fast-repetition rate fluoremeter (FRRF), FastOcean PTX with FastAct Laboratory system, Chelsea Technologies after Oxborough et al. (201	FRRF-alpha [relative electron transfer rate * (µmol photons m-2 s-1)-1]
31	Maximum photosynthesis rate, oxygen, per chlorophyll a	Pmax O2/Chl a	µmol/mg/h	Rokitta, Sebastian D	Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012), raw data obtained using a membrane-inlet mass spectrometer (MIMS) as described in Kottmeier, Rokitta & Rost (2016)	MIMS-Vmax [µmol O2 h-1 (mg Chl a)-1]
32	Light acclimation index	lk	µmol photons/m²/s	Rokitta, Sebastian D	Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012), raw data obtained using a membrane-inlet mass spectrometer (MIMS) as described in Kottmeier, Rokitta & Rost (2016)	MIMS-Ik [µmol photons m-2 s-1]
33	Initial slope of the photosynthesis-irradiance curve, relative electron transfer rate per unit light	alpha	1/(µmol photons/m²/s)	Rokitta, Sebastian D	Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012), raw data obtained using a membrane-inlet mass spectrometer (MIMS) as described in Kottmeier, Rokitta & Rost (2016)	MIMS-alpha [(µmol O2 h-1 (mg Chl a)-1) (µmol photons m-2 s-1)-1]
34	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	pH, total scale	pHT		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale, initial
36	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
37	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
38	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
39	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
40	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
41	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
42	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
43	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
44	pH, total scale	pHT		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale, final
45	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	final
46	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	final
47	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	final
48	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	final
49	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	final
50	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	final
51	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	final
52	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	final

Change history:

2025-05-09T08:11:26 – The parameters for species and keyword have been updated from "Phaeocystis pouchetii" to "Isochrysis sp." Additionally, all instances of "Phaeocystis pouchetii" in the Title and Abstract have been replaced with "Isochrysis sp."

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1580 data points

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