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Knight, Margaret A; Morris, J Jeffrey (2020): Seawater carbonate chemistry and growth of Prochlorococcus and Synechococcus strains [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.930555

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Abstract:
Anthropogenic CO2 emissions are projected to lower the pH of the ocean 0.3 units by 2100. Previous studies suggested that Prochlorococcus and Synechococcus, the numerically dominant phytoplankton in the oceans, have different responses to elevated CO2 that may result in a dramatic shift in their relative abundances in future oceans. Here we showed that the exponential growth rates of these two genera respond to future CO2 conditions in a manner similar to other cyanobacteria, but Prochlorococcus strains had significantly lower realized growth rates under elevated CO2 regimes due to poor survival after exposure to fresh culture media. Despite this, a Synechococcus strain was unable to outcompete a Prochlorococcus strain in co‐culture at elevated CO2. Under these conditions, Prochlorococcus' poor response to elevated CO2 disappeared, and Prochlorococcus' relative fitness showed negative frequency dependence, with both competitors having significant fitness advantages when initially rare. These experiments suggested that the two strains should be able to co‐exist indefinitely in co‐culture despite sharing nearly identical nutritional requirements. We speculate that negative frequency dependence exists due to reductive Black Queen evolution that has resulted in a passively mutualistic relationship analogous to that connecting Prochlorococcus with the “helper” heterotrophic microbes in its environment.
Keyword(s):
Bacteria; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Prochlorococcus sp.; Species interaction; Synechococcus sp.
Supplement to:
Knight, Margaret A; Morris, J Jeffrey (2020): Co-culture with Synechococcus facilitates growth of Prochlorococcus under ocean acidification conditions. Environmental Microbiology, 22(11), 4876-4889, https://doi.org/10.1111/1462-2920.15277
Original version:
Morris, J Jeffrey (2021): Data on laboratory cultures and statistical analysis code associated with the paper "Co-culture with Synechococcus facilitates the growth of Prochlorococcus under ocean acidification conditions" published in Environmental Microbiology [dataset]. Biological and Chemical Oceanography Data Management Office (BCO-DMO), https://doi.org/10.26008/1912/bco-dmo.839925.1
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-04-19.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeMorris, J Jeffreystudy
2FigureFigMorris, J Jeffrey
3ExperimentExpMorris, J Jeffrey
4SpeciesSpeciesMorris, J Jeffrey
5StrainStrainMorris, J Jeffrey
6ExperimentExpMorris, J JeffreyTogether
7IdentificationIDMorris, J JeffreyCulture
8TreatmentTreatMorris, J JeffreypCO2
9ReplicatesRepl#Morris, J Jeffrey
10CommentCommentMorris, J Jeffreysequential transfer
11Time in daysTimedaysMorris, J Jeffreybetween inoculation and transfer
12Cell densityCells#/mlMorris, J Jeffreyat the beginning of the transfer
13Cell densityCells#/mlMorris, J Jeffreyat the end of the transfer
14Growth rateµ1/dayMorris, J Jeffreymalthusian
15FrequencyFrequencyMorris, J JeffreyInitial
16FitnessFitnessMorris, J Jeffreycalculated as the ratio of the Prochlorococcus malthusian growth rate vs. the Synechococcus malthusian growth rate
17FitnessFitnessMorris, J Jeffreycalculated as the difference of the Prochlorococcus and Synechococcus malthusian growth rates, unit (1/day)
18Cell densityCells#/mlMorris, J JeffreyTotal cyanobacterial cell density at the beginning of the experiment
19Growth rateµ1/dayMorris, J Jeffreyexponential
20FitnessFitnessMorris, J Jeffreycalculated as the ratio of the Prochlorococcus exponential growth rate vs. the Synechococcus exponential growth rate
21FitnessFitnessMorris, J Jeffreycalculated as the difference of the Prochlorococcus and Synechococcus exponential growth rates, unit (1/day)
22Time in daysTimedaysMorris, J Jeffreylag
23SalinitySalMorris, J Jeffrey
24Temperature, waterTemp°CMorris, J Jeffrey
25Alkalinity, totalATµmol/lMorris, J JeffreyPotentiometric titration
26pHpHMorris, J JeffreyPotentiometric
27pH, standard deviationpH std dev±Morris, J JeffreyPotentiometric
28Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetppmvMorris, J JeffreyCalculated using seacarb
29Alkalinity, totalATµmol/kgMorris, J Jeffrey
30Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Carbon dioxide, standard deviationCO2 std dev±Yang, YanCalculated using seacarb after Orr et al. (2018)
33Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34Fugacity of carbon dioxide in seawater, standard deviationfCO2 std dev±Yang, YanCalculated using seacarb after Orr et al. (2018)
35Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
36Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Yang, YanCalculated using seacarb after Orr et al. (2018)
37Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
38Bicarbonate ion, standard deviation[HCO3]- std dev±Yang, YanCalculated using seacarb after Orr et al. (2018)
39Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
40Carbonate ion, standard deviation[CO3]2- std dev±Yang, YanCalculated using seacarb after Orr et al. (2018)
41Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
42Carbon, inorganic, dissolved, standard deviationDIC std dev±Yang, YanCalculated using seacarb after Orr et al. (2018)
43Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
44Aragonite saturation state, standard deviationOmega Arg std dev±Yang, YanCalculated using seacarb after Orr et al. (2018)
45Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
46Calcite saturation state, standard deviationOmega Cal std dev±Yang, YanCalculated using seacarb after Orr et al. (2018)
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
47250 data points

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