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Venn, Alexander A; Bernardet, Coralie; Chabenat, Apolline; Tambutté, Eric; Tambutté, Sylvie (2020): Seawater carbonate chemistry and half-time of calcein influx in the coral Stylophora pistillata [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.929464

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Abstract:
Coral calcification relies on the transport of ions and molecules to the extracellular calcifying medium (ECM). Little is known about paracellular transport (via intercellular junctions) in corals and other marine calcifiers. Here, we investigated whether the permeability of the paracellular pathway varied in different environmental conditions in the coral Stylophora pistillata. Using the fluorescent dye calcein, we characterised the dynamics of calcein influx from seawater to the ECM and showed that increases in paracellular permeability (leakiness) induced by hyperosmotic treatment could be detected by changes in calcein influx rates. We then used the calcein-imaging approach to investigate the effects of two environmental stressors on paracellular permeability: seawater acidification and temperature change. Under conditions of seawater acidification (pH 7.2) known to depress pH in the ECM and the calcifying cells of S. pistillata, we observed a decrease in half-times of calcein influx, indicating increased paracellular permeability. By contrast, high temperature (31°C) had no effect, whereas low temperature (20°C) caused decreases in paracellular permeability. Overall, our study establishes an approach to conduct further in vivo investigation of paracellular transport and suggests that changes in paracellular permeability could form an uncharacterised aspect of the physiological response of S. pistillata to seawater acidification.
Keyword(s):
Acid-base regulation; Animalia; Benthic animals; Benthos; Calcification/Dissolution; Cnidaria; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Laboratory strains; Not applicable; Other studied parameter or process; Single species; Stylophora pistillata
Supplement to:
Venn, Alexander A; Bernardet, Coralie; Chabenat, Apolline; Tambutté, Eric; Tambutté, Sylvie (2020): Paracellular transport to the coral calcifying medium: effects of environmental parameters. Journal of Experimental Biology, 223(17), jeb227074, https://doi.org/10.1242/jeb.227074
Original version:
Venn, Alexander A; Bernardet, Coralie; Chabenat, Apolline; Tambutté, Eric; Tambutté, Sylvie (2020): Half-times of calcein influx to the coral calcifying medium under conditions of seawater acidification and temperature variation. PANGAEA, https://doi.org/10.1594/PANGAEA.918127
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-03-15.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeVenn, Alexander Astudy
2SpeciesSpeciesVenn, Alexander A
3Registration number of speciesReg spec noVenn, Alexander A
4Uniform resource locator/link to referenceURL refVenn, Alexander AWoRMS Aphia ID
5TreatmentTreatVenn, Alexander A
6TreatmentTreatVenn, Alexander A
7Time in minutesTimeminVenn, Alexander AHalf time of calcein influx
8pH, extracellularpHeVenn, Alexander ApHECM: pH in the extracellular calcifying medium
9pH, intracellularpH inVenn, Alexander ApHi: pH in the calcifying cells
10DistanceDistanceµmVenn, Alexander ADistance to growing edge
11CommentCommentVenn, Alexander A
12Temperature, waterTemp°CVenn, Alexander A
13SalinitySalVenn, Alexander A
14Alkalinity, totalATµmol/kgVenn, Alexander APotentiometric titration
15pHpHVenn, Alexander ASpectrophotometrictotal scale
16Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
17Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
18Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
19Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
20Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
21Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
22Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
23Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
24Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
751 data points

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