Citation:

PANGAEA.929464

Name: Seawater carbonate chemistry and half-time of calcein influx in the coral Stylophora pistillata
Published: 2020
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Acid-base regulation; Animalia; Benthic animals; Benthos; Calcification/Dissolution; Cnidaria; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Laboratory strains; Not applicable; Other studied parameter or process; Single species; Stylophora pistillata

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Abstract:

Coral calcification relies on the transport of ions and molecules to the extracellular calcifying medium (ECM). Little is known about paracellular transport (via intercellular junctions) in corals and other marine calcifiers. Here, we investigated whether the permeability of the paracellular pathway varied in different environmental conditions in the coral Stylophora pistillata. Using the fluorescent dye calcein, we characterised the dynamics of calcein influx from seawater to the ECM and showed that increases in paracellular permeability (leakiness) induced by hyperosmotic treatment could be detected by changes in calcein influx rates. We then used the calcein-imaging approach to investigate the effects of two environmental stressors on paracellular permeability: seawater acidification and temperature change. Under conditions of seawater acidification (pH 7.2) known to depress pH in the ECM and the calcifying cells of S. pistillata, we observed a decrease in half-times of calcein influx, indicating increased paracellular permeability. By contrast, high temperature (31°C) had no effect, whereas low temperature (20°C) caused decreases in paracellular permeability. Overall, our study establishes an approach to conduct further in vivo investigation of paracellular transport and suggests that changes in paracellular permeability could form an uncharacterised aspect of the physiological response of S. pistillata to seawater acidification.

Keyword(s):

Acid-base regulation; Animalia; Benthic animals; Benthos; Calcification/Dissolution; Cnidaria; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Laboratory strains; Not applicable; Other studied parameter or process; Single species; Stylophora pistillata

Supplement to:

Venn, Alexander A; Bernardet, Coralie; Chabenat, Apolline; Tambutté, Eric; Tambutté, Sylvie (2020): Paracellular transport to the coral calcifying medium: effects of environmental parameters. Journal of Experimental Biology, 223(17), jeb227074, https://doi.org/10.1242/jeb.227074

Original version:

Venn, Alexander A; Bernardet, Coralie; Chabenat, Apolline; Tambutté, Eric; Tambutté, Sylvie (2020): Half-times of calcein influx to the coral calcifying medium under conditions of seawater acidification and temperature variation. PANGAEA, https://doi.org/10.1594/PANGAEA.918127

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-03-15.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Venn, Alexander A		study
2	Species	Species		Venn, Alexander A
3	Registration number of species	Reg spec no		Venn, Alexander A
4	Uniform resource locator/link to reference	URL ref		Venn, Alexander A		WoRMS Aphia ID
5	Treatment	Treat		Venn, Alexander A
6	Treatment	Treat		Venn, Alexander A
7	Time in minutes	Time	min	Venn, Alexander A		Half time of calcein influx
8	pH, extracellular	pHe		Venn, Alexander A		pHECM: pH in the extracellular calcifying medium
9	pH, intracellular	pH in		Venn, Alexander A		pHi: pH in the calcifying cells
10	Distance	Distance	µm	Venn, Alexander A		Distance to growing edge
11	Comment	Comment		Venn, Alexander A
12	Temperature, water	Temp	°C	Venn, Alexander A
13	Salinity	Sal		Venn, Alexander A
14	Alkalinity, total	AT	µmol/kg	Venn, Alexander A	Potentiometric titration
15	pH	pH		Venn, Alexander A	Spectrophotometric	total scale
16	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
17	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
18	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
19	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
20	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
21	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
22	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
23	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
24	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

751 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Treat	6 Treat	7 Time [min] (Half time of calcein influx)	8 pHe (pHECM: pH in the extracellula...)	9 pH in (pHi: pH in the calcifying cells)	10 Distance [µm] (Distance to growing edge)	11 Comment	12 Temp [°C]	13 Sal	14 AT [µmol/kg] (Potentiometric titration)	15 pH (total scale, Spectrophotometric)	16 CSC flag (Calculated using seacarb afte...)	17 CO2 [µmol/kg] (Calculated using seacarb afte...)	18 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	19 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	20 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	21 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	22 DIC [µmol/kg] (Calculated using seacarb afte...)	23 Omega Arg (Calculated using seacarb afte...)	24 Omega Cal (Calculated using seacarb afte...)
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Hyperosmotic treatment	glycine concentration: 200 mM	4.69				Data used for figure 4 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Hyperosmotic treatment	glycine concentration: 200 mM	4.49				Data used for figure 4 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Hyperosmotic treatment	glycine concentration: 200 mM	2.79				Data used for figure 4 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Hyperosmotic treatment	glycine concentration: 200 mM	3.61				Data used for figure 4 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Hyperosmotic treatment	glycine concentration: 200 mM	3.41				Data used for figure 4 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Seawater control	glycine concentration: 0 mM	6.05				Data used for figure 4 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Seawater control	glycine concentration: 0 mM	5.78				Data used for figure 4 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Seawater control	glycine concentration: 0 mM	7.97				Data used for figure 4 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Seawater control	glycine concentration: 0 mM	7.27				Data used for figure 4 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Seawater control	glycine concentration: 0 mM	7.31				Data used for figure 4 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Ambient seawater pH	pH: 8	6.83				Data used for figure 5 in related manuscript	25	38	2436.14	8.03	8	12.00	429.15	430.53	1867.73	231.32	2111.05	3.59	5.43
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Ambient seawater pH	pH: 8	6.63				Data used for figure 5 in related manuscript	25	38	2436.14	8.03	8	12.00	429.15	430.53	1867.73	231.32	2111.05	3.59	5.43
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Ambient seawater pH	pH: 8	7.66				Data used for figure 5 in related manuscript	25	38	2436.14	8.03	8	12.00	429.15	430.53	1867.73	231.32	2111.05	3.59	5.43
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Ambient seawater pH	pH: 8	5.57				Data used for figure 5 in related manuscript	25	38	2436.14	8.03	8	12.00	429.15	430.53	1867.73	231.32	2111.05	3.59	5.43
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Ambient seawater pH	pH: 8	8.01				Data used for figure 5 in related manuscript	25	38	2436.14	8.03	8	12.00	429.15	430.53	1867.73	231.32	2111.05	3.59	5.43
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Seawater acidification	pH: 7.2	4.52				Data used for figure 5 in related manuscript	25	38	2463.03	7.24	8	94.17	3369.08	3379.91	2349.23	46.67	2490.07	0.72	1.10
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Seawater acidification	pH: 7.2	6.16				Data used for figure 5 in related manuscript	25	38	2463.03	7.24	8	94.17	3369.08	3379.91	2349.23	46.67	2490.07	0.72	1.10
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Seawater acidification	pH: 7.2	4.24				Data used for figure 5 in related manuscript	25	38	2463.03	7.24	8	94.17	3369.08	3379.91	2349.23	46.67	2490.07	0.72	1.10
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Seawater acidification	pH: 7.2	5.06				Data used for figure 5 in related manuscript	25	38	2463.03	7.24	8	94.17	3369.08	3379.91	2349.23	46.67	2490.07	0.72	1.10
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Seawater acidification	pH: 7.2	6.01				Data used for figure 5 in related manuscript	25	38	2463.03	7.24	8	94.17	3369.08	3379.91	2349.23	46.67	2490.07	0.72	1.10
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Low temperature	temperature: 20	8.63				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Low temperature	temperature: 20	8.60				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Low temperature	temperature: 20	10.84				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Low temperature	temperature: 20	9.37				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Low temperature	temperature: 20	9.14				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Control temperature	temperature: 25	7.43				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Control temperature	temperature: 25	4.85				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Control temperature	temperature: 25	6.51				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Control temperature	temperature: 25	7.33				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Control temperature	temperature: 25	7.14				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Control temperature	temperature: 25	8.01				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	High temperature	temperature: 31	12.39				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	High temperature	temperature: 31	6.29				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	High temperature	temperature: 31	6.53				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	High temperature	temperature: 31	9.02				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	High temperature	temperature: 31	7.21				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	High temperature	temperature: 31	7.08				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	High temperature	temperature: 31	6.61				Data used for figure 6 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Low temperature	temperature: 20		8.12	7.22		Data used for figure 7 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Low temperature	temperature: 20		8.12	7.32		Data used for figure 7 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Low temperature	temperature: 20		8.24	7.33		Data used for figure 7 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Low temperature	temperature: 20		8.18	7.28		Data used for figure 7 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Control temperature	temperature: 25		8.27	7.27		Data used for figure 7 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Control temperature	temperature: 25		8.24	7.25		Data used for figure 7 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Control temperature	temperature: 25		8.18	7.32		Data used for figure 7 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Control temperature	temperature: 25		8.09	7.32		Data used for figure 7 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Control temperature	temperature: 25		8.00			Data used for figure 7 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	High temperature	temperature: 31		8.03	7.30		Data used for figure 7 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	High temperature	temperature: 31		8.10	7.25		Data used for figure 7 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	High temperature	temperature: 31		8.01	7.33		Data used for figure 7 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	High temperature	temperature: 31		8.11	7.34		Data used for figure 7 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	High temperature	temperature: 31		8.18	7.40		Data used for figure 7 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 1		7.48			25.8	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 1		5.63			60.7	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 1		6.23			83.2	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 1		4.86			71.7	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 2		6.36			243.0	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 2		6.24			111.0	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 2		4.17			113.0	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 2		6.36			31.1	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 3		8.92			48.6	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 3		8.22			67.9	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 3		8.83			79.9	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 3		7.89			90.5	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 3		9.07			101.0	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 4		6.30			45.9	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 4		6.92			130.0	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 4		6.48			98.3	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 5		6.42			67.7	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 5		8.18			95.3	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 5		7.20			56.7	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 6		7.79			99.1	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 6		7.70			135.0	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 6		7.24			160.0	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 6		6.49			209.0	Data used for figure 3 in related manuscript
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	Coral colony 6		9.55			79.3	Data used for figure 3 in related manuscript