Hanke, Inken; Hassenrück, Christiane; Kunzmann, Andreas; Aerts, Johan; Gärdes, Astrid (2019): Ontogenetic scale cortisol, body condition, and gut content analyses of juvenile milkfish (Chanos chanos) cultured in commercial marine cage systems [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.904040
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Published: 2019-07-18 • DOI registered: 2022-09-06
Abstract:
To quantify chronic stress levels in milkfish cultured within a typical commercial mariculture setting, we measured ontogenetic (OG) scale cortisol of milkfish sampled from four cage systems (F1-F4) with different geographic locations and management strategies. Sampling stations were located within a mariculture area (approx. 2.2 x 0.6 km) of the Guiguiwanen Channel, Bolinao (Philippines). Each station was sampled twice (time point I and II) with an intermediate time interval of 15 days. According to Aerts et al. (2015), ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) was used to analyze OG scale cortisol.
Additional to the stress-physiological data, environmental parameters (oxygen concentration, salinity) were recorded, and the gut content of the fish and the fish feed were analyzed for their carbon (C) and nitrogen (N) content, CN stable isotope signatures, presence of antibiotics (LC-MS/MS), organic matter (pyrolysis-GCMS, MetaboLights accession MTBLS1438), microbial community composition (16S amplicon sequencing, PRJEB33594, OTU table available here), and abundance of the virulence factor gene thermolabile hemolysin of Vibrio sp. (qPCR, primers: tlh-G-vibrio-0515-a-S-22: 5'-GCTGGTTCTTRGGDCAYTTCTC-3', tlh-G-vibrio-0750-a-A-22: 5'-TGGAACGCYACGGTTRTAGTTC-3'). Furthermore, the sampling took place as part of a larger sampling campaign within the ACUTE project (AquaCUlture practice in Tropical coastal Ecosystems: Understanding ecological and socio-economic consequences; funding: Leibniz SAW-2015-ZMT-4), during which also sediment microbial communities and environmental parameters were surveyed (sediment: doi: 10.1093/femsec/fiz006, SRA accession: SRP155505, doi:10.1594/PANGAEA.900758).
Related to:
Hanke, Inken; Hassenrück, Christiane; Ampe, Bart; Kunzmann, Andreas; Gärdes, Astrid; Aerts, Johan (2020): Chronic stress under commercial aquaculture conditions: Scale cortisol to identify and quantify potential stressors in milkfish (Chanos chanos) mariculture. Aquaculture, 526, 735352, https://doi.org/10.1016/j.aquaculture.2020.735352
Moncada, Chyrene; Hassenrück, Christiane; Gärdes, Astrid; Conaco, Cecilia (2019): Sediment and pore water measurements taken from a fish farming area in Bolinao, Philippines, 2017-06-10 and 2017-06-11 [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.900758
Further details:
Hanke, Inken; Hassenrück, Christiane; Kunzmann, Andreas; Aerts, Johan; Gärdes, Astrid (2021): Gut microbial communities of milkfish from open-cage mariculture [dataset]. European Nucleotide Archive (ENA), insdc:PRJEB33594
Coverage:
Median Latitude: 16.383955 * Median Longitude: 119.921657 * South-bound Latitude: 16.379720 * West-bound Longitude: 119.915700 * North-bound Latitude: 16.388020 * East-bound Longitude: 119.926830
Date/Time Start: 2017-05-12T00:00:00 * Date/Time End: 2017-06-05T00:00:00
Minimum DEPTH, water: 0.5 m * Maximum DEPTH, water: 20.5 m
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Hassenrück, Christiane | |||
| 2 | Sample code/label | Sample label | Hassenrück, Christiane | Sample title | ||
| 3 | Sample ID | Sample ID | Hassenrück, Christiane | Taxon ID | ||
| 4 | Sample comment | Sample comment | Hassenrück, Christiane | Sample description | ||
| 5 | DATE/TIME | Date/Time | Hassenrück, Christiane | Geocode – Sampling date | ||
| 6 | DEPTH, water | Depth water | m | Hassenrück, Christiane | Geocode – Approximate sampling depth (fish collected with hand net) | |
| 7 | Host | Host | Hassenrück, Christiane | Common name | ||
| 8 | Sample ID | Sample ID | Hassenrück, Christiane | Host taxid | ||
| 9 | Description | Description | Hassenrück, Christiane | Host body site | ||
| 10 | Mass | Mass | g | Hassenrück, Christiane | Host mass total | |
| 11 | Length | l | m | Hassenrück, Christiane | Host length, total length | |
| 12 | Length | l | m | Hassenrück, Christiane | Standard length | |
| 13 | Description | Description | Hassenrück, Christiane | Type of fish feed used at the time of sampling | ||
| 14 | Station label | Station | Hassenrück, Christiane | Name of sampling site used in manuscript | ||
| 15 | Time point, descriptive | Time point | Hassenrück, Christiane | Name of sampling timepoint used in manuscript | ||
| 16 | DEPTH, water | Depth water | m | Hassenrück, Christiane | Geocode – Water depth at the fish cage | |
| 17 | Size | Size | Hassenrück, Christiane | Approximate length of fish at stocking | ||
| 18 | Number | No | Hassenrück, Christiane | Approximate number of fish in the cage at time of stocking | ||
| 19 | Fish | Fish | #/m3 | Hassenrück, Christiane | Calculated | Approximately stocking density calculated from the the number of fish per cage and the approximate cage volume (F1-F3: approx. 1440m3; F4: approx. 1152m3) |
| 20 | Time in days | Time | days | Hassenrück, Christiane | Culture time of fish within the cage prior to sampling | |
| 21 | Condition factor | CF | Hassenrück, Christiane | Body condition | ||
| 22 | Liver, mass | Liver m | g | Hassenrück, Christiane | Weighted | |
| 23 | Cortisol | Cortisol | pg/mg | Hassenrück, Christiane | after Aerts et al. (2015) | Cortisol level in ontogenetic scales |
| 24 | Carbon, organic, total | TOC | % | Hassenrück, Christiane | Elemental analyzer, EuroVector, EA 3000 | |
| 25 | Nitrogen, total | TN | % | Hassenrück, Christiane | Elemental analyzer, EuroVector, EA 3000 | |
| 26 | Carbon/Nitrogen ratio | C/N | Hassenrück, Christiane | Calculated | ||
| 27 | δ13C, organic carbon | δ13C Corg | ‰ PDB | Hassenrück, Christiane | Elemental analyzer, EuroVector, EA 3000 | |
| 28 | δ15N, total dissolved nitrogen | δ15N TDN | ‰ air | Hassenrück, Christiane | Elemental analyzer, EuroVector, EA 3000 | |
| 29 | Gene copies | GCN | #/ng | Hassenrück, Christiane | Fluorescence-based quantitative real-time PCR (qPCR) | Thermolabile hemolysin (tlh) gene copies per ng extracted DNA |
| 30 | Gene copies | GCN | #/µl | Hassenrück, Christiane | Fluorescence-based quantitative real-time PCR (qPCR) | Thermolabile hemolysin (tlh) gene copies per 300µL of gut content |
| 31 | Sample comment | Sample comment | Hassenrück, Christiane | Fluorescence-based quantitative real-time PCR (qPCR) | Indicates if gene copy number is below limit of detection (LoD) or below limit of quantification (LoQ) as determined by the calibration of the qPCR essay with known quantities of gene copies |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
1155 data points
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- Hassenrück, C (2019): Physico-chemical characterization of the water column in the open-cage milkfish aquaculture area in Bolinao. https://doi.org/10.1594/PANGAEA.904134
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