Citation:

PANGAEA.904040

Name: Ontogenetic scale cortisol, body condition, and gut content analyses of juvenile milkfish (Chanos chanos) cultured in commercial marine cage systems
Published: 2019-07-18
License: https://creativecommons.org/licenses/by/4.0/
Keywords: marine aquaculture; microbial communities; Philippines

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Published: 2019-07-18 • DOI registered: 2022-09-06

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Abstract:

To quantify chronic stress levels in milkfish cultured within a typical commercial mariculture setting, we measured ontogenetic (OG) scale cortisol of milkfish sampled from four cage systems (F1-F4) with different geographic locations and management strategies. Sampling stations were located within a mariculture area (approx. 2.2 x 0.6 km) of the Guiguiwanen Channel, Bolinao (Philippines). Each station was sampled twice (time point I and II) with an intermediate time interval of 15 days. According to Aerts et al. (2015), ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) was used to analyze OG scale cortisol.

Additional to the stress-physiological data, environmental parameters (oxygen concentration, salinity) were recorded, and the gut content of the fish and the fish feed were analyzed for their carbon (C) and nitrogen (N) content, CN stable isotope signatures, presence of antibiotics (LC-MS/MS), organic matter (pyrolysis-GCMS, MetaboLights accession MTBLS1438), microbial community composition (16S amplicon sequencing, PRJEB33594, OTU table available here), and abundance of the virulence factor gene thermolabile hemolysin of Vibrio sp. (qPCR, primers: tlh-G-vibrio-0515-a-S-22: 5'-GCTGGTTCTTRGGDCAYTTCTC-3', tlh-G-vibrio-0750-a-A-22: 5'-TGGAACGCYACGGTTRTAGTTC-3'). Furthermore, the sampling took place as part of a larger sampling campaign within the ACUTE project (AquaCUlture practice in Tropical coastal Ecosystems: Understanding ecological and socio-economic consequences; funding: Leibniz SAW-2015-ZMT-4), during which also sediment microbial communities and environmental parameters were surveyed (sediment: doi: 10.1093/femsec/fiz006, SRA accession: SRP155505, doi:10.1594/PANGAEA.900758).

Keyword(s):

marine aquaculture; microbial communities; Philippines

Related to:

Hanke, Inken; Hassenrück, Christiane; Ampe, Bart; Kunzmann, Andreas; Gärdes, Astrid; Aerts, Johan (2020): Chronic stress under commercial aquaculture conditions: Scale cortisol to identify and quantify potential stressors in milkfish (Chanos chanos) mariculture. Aquaculture, 526, 735352, https://doi.org/10.1016/j.aquaculture.2020.735352

Moncada, Chyrene; Hassenrück, Christiane; Gärdes, Astrid; Conaco, Cecilia (2019): Sediment and pore water measurements taken from a fish farming area in Bolinao, Philippines, 2017-06-10 and 2017-06-11 [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.900758

Further details:

Chemical compounds table of milkfish field B17 used in Hassenrück (2020)

Fish feed information used in Hassenrück (2020)

OTU information used in Hassenrück (2020)

OTU sequences of microbial communities from milkfish field B17 used in Hassenrück (2020)

Hanke, Inken; Hassenrück, Christiane; Kunzmann, Andreas; Aerts, Johan; Gärdes, Astrid (2021): Gut microbial communities of milkfish from open-cage mariculture [dataset]. European Nucleotide Archive (ENA), insdc:PRJEB33594

Coverage:

Median Latitude: 16.383955 * Median Longitude: 119.921657 * South-bound Latitude: 16.379720 * West-bound Longitude: 119.915700 * North-bound Latitude: 16.388020 * East-bound Longitude: 119.926830

Date/Time Start: 2017-05-12T00:00:00 * Date/Time End: 2017-06-05T00:00:00

Minimum DEPTH, water: 0.5 m * Maximum DEPTH, water: 20.5 m

Event(s):

Guiguiwanen_channel-F1 * Latitude: 16.383080 * Longitude: 119.915700 * Location: Philippines

Guiguiwanen_channel-F2 * Latitude: 16.388020 * Longitude: 119.918170 * Location: Philippines

Guiguiwanen_channel-F3 * Latitude: 16.385000 * Longitude: 119.925930 * Location: Philippines

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Hassenrück, Christiane
2	Sample code/label	Sample label		Hassenrück, Christiane		Sample title
3	Sample ID	Sample ID		Hassenrück, Christiane		Taxon ID
4	Sample comment	Sample comment		Hassenrück, Christiane		Sample description
5	DATE/TIME	Date/Time		Hassenrück, Christiane		Geocode – Sampling date
6	DEPTH, water	Depth water	m	Hassenrück, Christiane		Geocode – Approximate sampling depth (fish collected with hand net)
7	Host	Host		Hassenrück, Christiane		Common name
8	Sample ID	Sample ID		Hassenrück, Christiane		Host taxid
9	Description	Description		Hassenrück, Christiane		Host body site
10	Mass	Mass	g	Hassenrück, Christiane		Host mass total
11	Length	l	m	Hassenrück, Christiane		Host length, total length
12	Length	l	m	Hassenrück, Christiane		Standard length
13	Description	Description		Hassenrück, Christiane		Type of fish feed used at the time of sampling
14	Station label	Station		Hassenrück, Christiane		Name of sampling site used in manuscript
15	Time point, descriptive	Time point		Hassenrück, Christiane		Name of sampling timepoint used in manuscript
16	DEPTH, water	Depth water	m	Hassenrück, Christiane		Geocode – Water depth at the fish cage
17	Size	Size		Hassenrück, Christiane		Approximate length of fish at stocking
18	Number	No		Hassenrück, Christiane		Approximate number of fish in the cage at time of stocking
19	Fish	Fish	#/m³	Hassenrück, Christiane	Calculated	Approximately stocking density calculated from the the number of fish per cage and the approximate cage volume (F1-F3: approx. 1440m3; F4: approx. 1152m3)
20	Time in days	Time	days	Hassenrück, Christiane		Culture time of fish within the cage prior to sampling
21	Condition factor	CF		Hassenrück, Christiane		Body condition
22	Liver, mass	Liver m	g	Hassenrück, Christiane	Weighted
23	Cortisol	Cortisol	pg/mg	Hassenrück, Christiane	after Aerts et al. (2015)	Cortisol level in ontogenetic scales
24	Carbon, organic, total	TOC	%	Hassenrück, Christiane	Elemental analyzer, EuroVector, EA 3000
25	Nitrogen, total	TN	%	Hassenrück, Christiane	Elemental analyzer, EuroVector, EA 3000
26	Carbon/Nitrogen ratio	C/N		Hassenrück, Christiane	Calculated
27	δ13C, organic carbon	δ13C Corg	‰ PDB	Hassenrück, Christiane	Elemental analyzer, EuroVector, EA 3000
28	δ15N, total dissolved nitrogen	δ15N TDN	‰ air	Hassenrück, Christiane	Elemental analyzer, EuroVector, EA 3000
29	Gene copies	GCN	#/ng	Hassenrück, Christiane	Fluorescence-based quantitative real-time PCR (qPCR)	Thermolabile hemolysin (tlh) gene copies per ng extracted DNA
30	Gene copies	GCN	#/µl	Hassenrück, Christiane	Fluorescence-based quantitative real-time PCR (qPCR)	Thermolabile hemolysin (tlh) gene copies per 300µL of gut content
31	Sample comment	Sample comment		Hassenrück, Christiane	Fluorescence-based quantitative real-time PCR (qPCR)	Indicates if gene copy number is below limit of detection (LoD) or below limit of quantification (LoQ) as determined by the calibration of the qPCR essay with known quantities of gene copies

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Size:

1155 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 Sample label (Sample title)	3 Sample ID (Taxon ID)	4 Sample comment (Sample description)	5 Date/Time (Sampling date)	6 Depth water [m] (Approximate sampling depth (f...)	7 Host (Common name)	8 Sample ID (Host taxid)	9 Description (Host body site)	10 Mass [g] (Host mass total)	11 l [m] (Host length, total length)	12 l [m] (Standard length)	13 Description (Type of fish feed used at the...)	14 Station (Name of sampling site used in...)	15 Time point (Name of sampling timepoint us...)	16 Depth water [m] (Water depth at the fish cage)	17 Size (Approximate length of fish at...)	18 No (Approximate number of fish in...)	19 Fish [#/m³] (Approximately stocking densit...)	20 Time [days] (Culture time of fish within t...)	21 CF (Body condition)	22 Liver m [g] (Weighted)	23 Cortisol [pg/mg] (Cortisol level in ontogenetic...)	24 TOC [%] (Elemental analyzer, EuroVecto...)	25 TN [%] (Elemental analyzer, EuroVecto...)	26 C/N (Calculated)	27 δ13C Corg [‰ PDB] (Elemental analyzer, EuroVecto...)	28 δ15N TDN [‰ air] (Elemental analyzer, EuroVecto...)	29 GCN [#/ng] (Thermolabile hemolysin (tlh) ...)	30 GCN [#/µl] (Thermolabile hemolysin (tlh) ...)	31 Sample comment (Indicates if gene copy number...)
Guiguiwanen_channel-F1	F1_C_1	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-12	0.5	milkfish	29144	Intestine [FMA:7199]	119.85	0.225	0.184	Aquaration Fingerling Crumble B-Meg	F1	I	18.8	5-6 cm	46000	32	93	1.052181070	2.2843	0.056	41.271	7.358	5.608997010	-25.448	3.228	3068	74650
Guiguiwanen_channel-F1	F1_C_2	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-12	0.5	milkfish	29144	Intestine [FMA:7199]	108.37	0.223	0.180	Aquaration Fingerling Crumble B-Meg	F1	I	18.8	5-6 cm	46000	32	93	0.977224810	1.8686	0.059						4	6630	<LOQ
Guiguiwanen_channel-F1	F1_C_3	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-12	0.5	milkfish	29144	Intestine [FMA:7199]	114.62	0.225	0.182	Aquaration Fingerling Crumble B-Meg	F1	I	18.8	5-6 cm	46000	32	93	1.006266118	2.3353	0.049						3	5313	<LOQ
Guiguiwanen_channel-F1	F1_C_4	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-12	0.5	milkfish	29144	Intestine [FMA:7199]	99.21	0.214	0.173	Aquaration Fingerling Crumble B-Meg	F1	I	18.8	5-6 cm	46000	32	93	1.012311405	1.9267	0.037	35.488	4.694	7.560289732	-25.435	3.324	6	3151	<LOQ
Guiguiwanen_channel-F1	F1_C_5	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-12	0.5	milkfish	29144	Intestine [FMA:7199]	126.02	0.230	0.189	Aquaration Fingerling Crumble B-Meg	F1	I	18.8	5-6 cm	46000	32	93	1.035752445	2.4867	0.068						234	451333
Guiguiwanen_channel-F1	F1_C_6	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-12	0.5	milkfish	29144	Intestine [FMA:7199]	104.95	0.220	0.179	Aquaration Fingerling Crumble B-Meg	F1	I	18.8	5-6 cm	46000	32	93	0.985631104	2.2458	0.038	35.517	5.066	7.010856692	-25.429	3.390	411	85642
Guiguiwanen_channel-F1	F1_C_7	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-27	0.5	milkfish	29144	Intestine [FMA:7199]	222.86	0.287	0.232	Aquaration Juveniles Pellets B-Meg	F1	II	20.5	5-6 cm	46000	32	108	0.942728064	2.7901	0.304						115	156858	<LOQ
Guiguiwanen_channel-F1	F1_C_8	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-27	0.5	milkfish	29144	Intestine [FMA:7199]	181.10	0.268	0.215	Aquaration Juveniles Pellets B-Meg	F1	II	20.5	5-6 cm	46000	32	108	0.940836306	3.0971	0.129	39.027	8.874	4.397903989	-24.140	4.375	726	1176583
Guiguiwanen_channel-F1	F1_C_9	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-27	0.5	milkfish	29144	Intestine [FMA:7199]	206.30	0.272	0.221	Aquaration Juveniles Pellets B-Meg	F1	II	20.5	5-6 cm	46000	32	108	1.025162038	3.8103	0.146	40.833	8.149	5.010798871	-24.701	3.830	532	1050000
Guiguiwanen_channel-F1	F1_C_10	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-27	0.5	milkfish	29144	Intestine [FMA:7199]	204.96	0.270	0.222	Aquaration Juveniles Pellets B-Meg	F1	II	20.5	5-6 cm	46000	32	108	1.041304679	4.1441	0.138						2759	4867333
Guiguiwanen_channel-F1	F1_C_11	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-27	0.5	milkfish	29144	Intestine [FMA:7199]	203.68	0.271	0.220	Aquaration Juveniles Pellets B-Meg	F1	II	20.5	5-6 cm	46000	32	108	1.023388456	4.1544	0.208	41.432	9.760	4.245081967	-24.619	3.407	8135555	17905000000
Guiguiwanen_channel-F1	F1_C_12	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-27	0.5	milkfish	29144	Intestine [FMA:7199]	258.12	0.302	0.246	Aquaration Juveniles Pellets B-Meg	F1	II	20.5	5-6 cm	46000	32	108	0.937132129	5.1708	0.305						0	0	<LOD
Guiguiwanen_channel-F2	F2_C_1	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-21	0.5	milkfish	29144	Intestine [FMA:7199]	82.91	0.203	0.165	Santeh fingerling crumble (1)/ New Hope Fish grower feeds (crumble 2)	F2	I	13.1	5-6 cm	45000	31	80	0.991103024	1.4635	0.037	36.669	5.160	7.106395349	-23.284	3.347	0	0	<LOD
Guiguiwanen_channel-F2	F2_C_2	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-21	0.5	milkfish	29144	Intestine [FMA:7199]	90.10	0.203	0.166	Santeh fingerling crumble (1)/ New Hope Fish grower feeds (crumble 2)	F2	I	13.1	5-6 cm	45000	31	80	1.077052014	1.8487	0.055						34	52283	<LOQ
Guiguiwanen_channel-F2	F2_C_3	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-21	0.5	milkfish	29144	Intestine [FMA:7199]	79.67	0.198	0.162	Santeh fingerling crumble (1)/ New Hope Fish grower feeds (crumble 2)	F2	I	13.1	5-6 cm	45000	31	80	1.026358885	1.3337	0.025	35.755	4.837	7.391978499	-24.219	3.232	607	1051667
Guiguiwanen_channel-F2	F2_C_4	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-21	0.5	milkfish	29144	Intestine [FMA:7199]	84.30	0.203	0.166	Santeh fingerling crumble (1)/ New Hope Fish grower feeds (crumble 2)	F2	I	13.1	5-6 cm	45000	31	80	1.007719032	1.4772	0.030	36.077	4.336	8.320341328	-23.871	3.057	742	1391667
Guiguiwanen_channel-F2	F2_C_5	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-21	0.5	milkfish	29144	Intestine [FMA:7199]	95.15	0.207	0.172	Santeh fingerling crumble (1)/ New Hope Fish grower feeds (crumble 2)	F2	I	13.1	5-6 cm	45000	31	80	1.072748106	1.7325	0.039						2753	5060000
Guiguiwanen_channel-F2	F2_C_6	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture, PCR failed (no sequencing data)	2017-05-21	0.5	milkfish	29144	Intestine [FMA:7199]	72.94	0.188	0.153	Santeh fingerling crumble (1)/ New Hope Fish grower feeds (crumble 2)	F2	I	13.1	5-6 cm	45000	31	80	1.097721603	1.6495	0.032
Guiguiwanen_channel-F2	F2_C_7	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-06-05	0.5	milkfish	29144	Intestine [FMA:7199]	109.71	0.225	0.180	New Hope Fish grower feeds (crumble 2)/ Santeh Starter pellets	F2	II	12.9	5-6 cm	45000	31	95	0.963160494	1.6804	0.058						2562	2781667
Guiguiwanen_channel-F2	F2_C_8	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-06-05	0.5	milkfish	29144	Intestine [FMA:7199]	116.38	0.231	0.188	New Hope Fish grower feeds (crumble 2)/ Santeh Starter pellets	F2	II	12.9	5-6 cm	45000	31	95	0.944153078	1.7774	0.065	37.264	4.979	7.484233782	-24.205	3.415	2841	3089167
Guiguiwanen_channel-F2	F2_C_9	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-06-05	0.5	milkfish	29144	Intestine [FMA:7199]	148.29	0.244	0.200	New Hope Fish grower feeds (crumble 2)/ Santeh Starter pellets	F2	II	12.9	5-6 cm	45000	31	95	1.020804054	2.5864	0.105	37.836	5.814	6.507739938	-23.926	3.503	543	921750
Guiguiwanen_channel-F2	F2_C_10	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-06-05	0.5	milkfish	29144	Intestine [FMA:7199]	103.37	0.219	0.180	New Hope Fish grower feeds (crumble 2)/ Santeh Starter pellets	F2	II	12.9	5-6 cm	45000	31	95	0.984151983	1.4235	0.053						0	0	<LOD
Guiguiwanen_channel-F2	F2_C_11	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-06-05	0.5	milkfish	29144	Intestine [FMA:7199]	111.42	0.230	0.188	New Hope Fish grower feeds (crumble 2)/ Santeh Starter pellets	F2	II	12.9	5-6 cm	45000	31	95	0.915755733	1.6525	0.066						1887	4995833
Guiguiwanen_channel-F2	F2_C_12	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-06-05	0.5	milkfish	29144	Intestine [FMA:7199]	120.66	0.231	0.192	New Hope Fish grower feeds (crumble 2)/ Santeh Starter pellets	F2	II	12.9	5-6 cm	45000	31	95	0.978875325	2.0569	0.062	37.472	6.017	6.227688217	-24.381	3.385	0	0	<LOD
Guiguiwanen_channel-F3	F3_C_1	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-19	0.5	milkfish	29144	Intestine [FMA:7199]	121.06	0.231	0.187	Pinoy Starter Pellets	F3	I	13.7	5-6 cm	45000	31	94	0.982120395	2.7175	0.064	34.460	5.827	5.913849322	-23.832	3.947	0	0	<LOD
Guiguiwanen_channel-F3	F3_C_2	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-19	0.5	milkfish	29144	Intestine [FMA:7199]	107.89	0.221	0.181	Pinoy Starter Pellets	F3	I	13.7	5-6 cm	45000	31	94	0.999549651	2.0326	0.057						0	0	<LOD
Guiguiwanen_channel-F3	F3_C_3	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-19	0.5	milkfish	29144	Intestine [FMA:7199]	150.21	0.250	0.202	Pinoy Starter Pellets	F3	I	13.7	5-6 cm	45000	31	94	0.961344000	3.1958	0.087	35.816	6.947	5.155606737	-23.988	4.240	8	6433	<LOQ
Guiguiwanen_channel-F3	F3_C_4	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-19	0.5	milkfish	29144	Intestine [FMA:7199]	117.58	0.229	0.187	Pinoy Starter Pellets	F3	I	13.7	5-6 cm	45000	31	94	0.979099906	3.2671	0.054						29	23196	<LOQ
Guiguiwanen_channel-F3	F3_C_5	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-19	0.5	milkfish	29144	Intestine [FMA:7199]	125.02	0.230	0.189	Pinoy Starter Pellets	F3	I	13.7	5-6 cm	45000	31	94	1.027533492	3.1768	0.067	38.383	5.659	6.782647111	-25.086	4.106	36	30458
Guiguiwanen_channel-F3	F3_C_6	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-19	0.5	milkfish	29144	Intestine [FMA:7199]	77.55	0.200	0.165	Pinoy Starter Pellets	F3	I	13.7	5-6 cm	45000	31	94	0.969375000	1.5962	0.028						19	15686	<LOQ
Guiguiwanen_channel-F3	F3_C_7	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-06-03	0.5	milkfish	29144	Intestine [FMA:7199]	243.65	0.298	0.241	Pinoy Starter Pellets	F3	II	12.9	5-6 cm	45000	31	109	0.920698898	3.5468	0.138	38.562	7.723	4.993137382	-23.244	4.300	0	0	<LOD
Guiguiwanen_channel-F3	F3_C_8	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-06-03	0.5	milkfish	29144	Intestine [FMA:7199]	177.19	0.260	0.209	Pinoy Starter Pellets	F3	II	12.9	5-6 cm	45000	31	109	1.008136095	3.1974	0.145						0	0	<LOD
Guiguiwanen_channel-F3	F3_C_9	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-06-03	0.5	milkfish	29144	Intestine [FMA:7199]	134.52	0.244	0.200	Pinoy Starter Pellets	F3	II	12.9	5-6 cm	45000	31	109	0.926013631	3.3382	0.086	37.497	6.664	5.626800720	-23.636	4.425	0	0	<LOD
Guiguiwanen_channel-F3	F3_C_10	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-06-03	0.5	milkfish	29144	Intestine [FMA:7199]	192.57	0.281	0.230	Pinoy Starter Pellets	F3	II	12.9	5-6 cm	45000	31	109	0.867899965	3.7437	0.181						0	0	<LOD
Guiguiwanen_channel-F3	F3_C_11	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-06-03	0.5	milkfish	29144	Intestine [FMA:7199]	112.92	0.232	0.190	Pinoy Starter Pellets	F3	II	12.9	5-6 cm	45000	31	109	0.904288306	2.1175	0.058	33.799	7.238	4.669660127	-23.083	4.867	0	0	<LOD
Guiguiwanen_channel-F3	F3_C_12	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-06-03	0.5	milkfish	29144	Intestine [FMA:7199]	118.30	0.232	0.192	Pinoy Starter Pellets	F3	II	12.9	5-6 cm	45000	31	109	0.947372535	1.8906	0.065						0	0	<LOD
Guiguiwanen_channel-F4	F4_C_1	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-14	0.5	milkfish	29144	Intestine [FMA:7199]	241.91	0.281	0.230	Aquaration Fingerling Pellets B-Meg	F4	I	8.8	8-11 cm	55000	48	76	1.090272007	6.6017	0.118	40.051	5.722	6.999475708	-25.506	3.214	0	0	<LOD
Guiguiwanen_channel-F4	F4_C_2	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-14	0.5	milkfish	29144	Intestine [FMA:7199]	187.47	0.260	0.214	Aquaration Fingerling Pellets B-Meg	F4	I	8.8	8-11 cm	55000	48	76	1.066624943	6.2790	0.083	35.639	5.040	7.071230159	-25.185	3.435	0	0	<LOD
Guiguiwanen_channel-F4	F4_C_3	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-14	0.5	milkfish	29144	Intestine [FMA:7199]	172.04	0.253	0.208	Aquaration Fingerling Pellets B-Meg	F4	I	8.8	8-11 cm	55000	48	76	1.062350607	4.8599	0.075						1	1702	<LOQ
Guiguiwanen_channel-F4	F4_C_4	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-14	0.5	milkfish	29144	Intestine [FMA:7199]	240.77	0.278	0.225	Aquaration Fingerling Pellets B-Meg	F4	I	8.8	8-11 cm	55000	48	76	1.120644812	7.2581	0.134						0	0	<LOD
Guiguiwanen_channel-F4	F4_C_5	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-14	0.5	milkfish	29144	Intestine [FMA:7199]	178.25	0.256	0.210	Aquaration Fingerling Pellets B-Meg	F4	I	8.8	8-11 cm	55000	48	76	1.062452793	5.1755	0.137						0	0	<LOD
Guiguiwanen_channel-F4	F4_C_6	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-14	0.5	milkfish	29144	Intestine [FMA:7199]	232.17	0.277	0.228	Aquaration Fingerling Pellets B-Meg	F4	I	8.8	8-11 cm	55000	48	76	1.092362529	5.6995	0.166	38.814	5.512	7.041727141	-25.451	3.281	0	0	<LOD
Guiguiwanen_channel-F4	F4_C_7	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-29	0.5	milkfish	29144	Intestine [FMA:7199]	218.51	0.278	0.228	Aquaration Juveniles Pellets B-Meg	F4	II	8.6	8-11 cm	55000	48	91	1.017037413	3.2292	0.143	36.383	5.507	6.606682404	-24.876	3.825	0	0	<LOD
Guiguiwanen_channel-F4	F4_C_8	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-29	0.5	milkfish	29144	Intestine [FMA:7199]	325.67	0.305	0.253	Aquaration Juveniles Pellets B-Meg	F4	II	8.6	8-11 cm	55000	48	91	1.147831757	7.8397	0.177						5	8315	<LOQ
Guiguiwanen_channel-F4	F4_C_9	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-29	0.5	milkfish	29144	Intestine [FMA:7199]	313.41	0.300	0.248	Aquaration Juveniles Pellets B-Meg	F4	II	8.6	8-11 cm	55000	48	91	1.160777778	7.9063	0.294	37.148	4.495	8.264293660	-25.519	3.474	357	728833
Guiguiwanen_channel-F4	F4_C_10	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-29	0.5	milkfish	29144	Intestine [FMA:7199]	358.94	0.324	0.273	Aquaration Juveniles Pellets B-Meg	F4	II	8.6	8-11 cm	55000	48	91	1.055326461	6.9866	0.199	38.548	4.623	8.338308458	-25.296	3.436	8542	12871667
Guiguiwanen_channel-F4	F4_C_11	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-29	0.5	milkfish	29144	Intestine [FMA:7199]	290.85	0.301	0.250	Aquaration Juveniles Pellets B-Meg	F4	II	8.6	8-11 cm	55000	48	91	1.066521418	7.1470	0.196						0	0	<LOD
Guiguiwanen_channel-F4	F4_C_12	1602388	content of the distal 10cm of the milkfish intestine, milkfish from open-cage mariculture	2017-05-29	0.5	milkfish	29144	Intestine [FMA:7199]	375.69	0.313	0.265	Aquaration Juveniles Pellets B-Meg	F4	II	8.6	8-11 cm	55000	48	91	1.225170758	6.3385	0.268						977	1705667