Hanke, Inken; Reinwald, Hannes; Hassenrück, Christiane; Kunzmann, Andreas; Aerts, Johan; Gärdes, Astrid (2020): Scale cortisol, biomarkers, and gut microbiome of milkfish (Chanos chanos) experimentally exposed to thermal stress [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.919971
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Abstract:
Milkfish (Chanos chanos) is one of the most important aquaculture species in Asian countries. These teleost fish are traditionally cultured in outdoor-based systems and therefore have to cope with daily and/or seasonally changing environmental conditions. Temperature changes beyond the optimal range of a fish species are known to induce an endocrine stress response resulting in the release of cortisol via the hypothalamic-pituitary-interrenal axis. Moreover, (thermal) stress induces glucocorticoid-mediated changes in the fish's energy metabolism to cope with the stressor(s) and regain homeostasis. Long-term elevations of cortisol are known to be detrimental for fish performance. In this study, we investigated the stress response of juvenile milkfish, which were exposed to a gradual temperature increase of 1°C per day over 7 days in the range from 26°C to 33°C, followed by an exposure to constant 33°C for 21 days. We quantified ontogenetic (OG) and regenerated (RG) scale cortisol to evaluate chronic stress. To investigate metabolic implications and oxidative stress response, activity levels of key enzymes involved in metabolic (isocitrate dehydrogenase - IDH, lactate dehydrogenase - LDH, electron transfer system - ETS) and antioxidant (superoxide dismutase - SOD, catalase - CAT) related pathways were quantified. Furthermore, we measured available energy resources (protein, carbohydrates, lipids) and potential cellular damage due to oxidative stress (lipid peroxidation - LPO). Finally, changes in the gut microbiome of the milkfish related to the temperature stress were analyzed to elucidate their role in the stress response and interactions with physiological parameters.
This study is part of the ACUTE project (AquaCUlture practice in Tropical coastal Ecosystems - Understanding ecological and socio-economic consequences) funded by the Leibniz Association grant SAW-2015-ZMT-4. It is associated with the following publications: Hanke et al., 2019 (doi:10.1016/j.aquaculture.2018.09.016) and Hassenrück et al., 2020 (doi:10.3390/microorganisms9010005). The final OTU table and statistical analysis scripts for Hassenrück et al., 2020 are supplied as further details to this data set.
Related to:
Hanke, Inken; Ampe, Bart; Kunzmann, Andreas; Gärdes, Astrid; Aerts, Johan (2019): Thermal stress response of juvenile milkfish (Chanos chanos) quantified by ontogenetic and regenerated scale cortisol. Aquaculture, 500, 24-30, https://doi.org/10.1016/j.aquaculture.2018.09.016
Hassenrück, Christiane; Reinwald, Hannes; Kunzmann, Andreas; Tiedemann, Inken; Gärdes, Astrid (2020): Effects of Thermal Stress on the Gut Microbiome of Juvenile Milkfish (Chanos chanos). Microorganisms, 9(1), 5, https://doi.org/10.3390/microorganisms9010005
Further details:
Project(s):
Funding:
Leibniz Association (Leibniz Association), grant/award no. SAW-2015-ZMT-4: Aquaculture practice in tropical coastal ecosystems - understanding ecological and socio-economic consequences
Coverage:
Latitude: 53.107804 * Longitude: 8.845476
Date/Time Start: 2017-01-11T00:00:00 * Date/Time End: 2017-02-02T00:00:00
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Sample ID | Sample ID | Hanke, Inken | |||
| 2 | DATE/TIME | Date/Time | Hanke, Inken | Geocode – collection date | ||
| 3 | Sample material | Samp mat | Hanke, Inken | for microbial analysis | ||
| 4 | Treatment: temperature description | T:Temp descr | Hanke, Inken | Control: 26°C, no temperature change; High: gradual temperature increase of 1°C per day in the range from 26 to 33°C between d-7 and d0, followed by 21 days at constant 33°C | ||
| 5 | Tank number | Tank No | Hanke, Inken | |||
| 6 | Time point, descriptive | Time point | Hanke, Inken | Timepoint after the start of the experiment that the fish and water were sampled for microbial analysis (Sampling events: d-7: immediately before the start of the gradual temperature increase in the high temperature treatment; d0: immediately after the gradual temperature increase in high temperature treatment; d7: after 7 days at constant elevated temperature in the high temperature treatment; d14: after 14 days at constant elevated temperature in the high temperature treatment; d21: end of the experiment after 21 days at constant elevated temperature in the high temperature treatment | ||
| 7 | Chanos chanos, mass | C. chanos m | g | Hanke, Inken | Fish, wet weighted | Weight of the fish at d-7, corresponding MIxS parameter: host_tot_mass |
| 8 | Chanos chanos, length | C. chanos l | cm | Hanke, Inken | Fish measuring board | Total length of the fish at d-7, corresponding MIxS parameter: host_length |
| 9 | Chanos chanos, mass | C. chanos m | g | Hanke, Inken | Fish, wet weighted | Weight of the fish at d0, corresponding MIxS parameter: host_tot_mass |
| 10 | Chanos chanos, length | C. chanos l | cm | Hanke, Inken | Fish measuring board | Total length of the fish at d0, corresponding MIxS parameter: host_length |
| 11 | Chanos chanos, mass | C. chanos m | g | Hanke, Inken | Fish, wet weighted | Weight of the fish at d7, corresponding MIxS parameter: host_tot_mass |
| 12 | Chanos chanos, length | C. chanos l | cm | Hanke, Inken | Fish measuring board | Total length of the fish at d7, corresponding MIxS parameter: host_length |
| 13 | Chanos chanos, mass | C. chanos m | g | Hanke, Inken | Fish, wet weighted | Weight of the fish at d14, corresponding MIxS parameter: host_tot_mass |
| 14 | Chanos chanos, length | C. chanos l | cm | Hanke, Inken | Fish measuring board | Total length of the fish at d14, corresponding MIxS parameter: host_length |
| 15 | Chanos chanos, mass | C. chanos m | g | Hanke, Inken | Fish, wet weighted | Weight of the fish at d21 days, corresponding MIxS parameter: host_tot_mass |
| 16 | Chanos chanos, length | C. chanos l | cm | Hanke, Inken | Fish measuring board | Total length of the fish at d21, corresponding MIxS parameter: host_length |
| 17 | Cortisol | Cortisol | pg/mg | Hanke, Inken | after Aerts et al. (2015) | Ontogenetic scale cortisol at d-7, orig. unit: [ug kg-1] |
| 18 | Cortisol | Cortisol | pg/mg | Hanke, Inken | after Aerts et al. (2015) | Ontogenetic scale cortisol at d0, orig. unit: [ug kg-1] |
| 19 | Cortisol | Cortisol | pg/mg | Hanke, Inken | after Aerts et al. (2015) | Regenerated scale cortisol at d21, orig. unit: [ug kg-1] |
| 20 | Lactate dehydrogenase activity, per protein mass | LDH/prot | nmol/min/mg | Hanke, Inken | described by Vassault (1983) and adapted by Diamantino et al. (2001) | Activity of lactate dehydrogenase in muscle tissue [nmol (min mg protein)-1] |
| 21 | Isocitrate dehydrogenase activity, per protein mass | IDH/prot | nmol/min/mg | Hanke, Inken | described by Ellis and Goldberg (1971) and adapted to microplate usage by Lima et al. (2007) | Activity of isocitrate dehydrogenase in muscle tissue [nmol (min mg protein)-1] |
| 22 | Proteins, per wet mass | Protein wm | mJ/mg | Hanke, Inken | Bradford method (1976) | Total protein in muscle tissue [mJ (mg wet weight)-1] |
| 23 | Carbohydrates, per wet mass | CHO wm | mJ/mg | Hanke, Inken | after De Coen and Janssen (1997) | Total carbohydrates in muscle tissue [mJ (mg wet weight)-1] |
| 24 | Lipids, per wet mass | Lipid wm | mJ/mg | Hanke, Inken | Extraction of Lipids after Bligh & Dyer (1959) | Total lipids in muscle tissue [mJ (mg wet weight)-1] |
| 25 | Available energy, per wet mass | Ea/WM | mJ/mg | Hanke, Inken | Calculated (sum of Protein, Carbohydrates, Lipids) | Energy available as calculated by the sum of total protein, carbohydrates, and lipids in muscle tissue [mJ (mg wet weight)-1] |
| 26 | Electron transport system activity, per wet mass | ETS | mJ/h/mg | Hanke, Inken | after De Coen and Janssen (1997) | Activity of electron transfer system (ETS) in muscle tissue [mJ (h mg wet weight)-1] |
| 27 | Cellular energy allocation | CEA | Hanke, Inken | Calculated according to Verslycke et al. (2004) | Ratio of EA and ETS | |
| 28 | Catalase activity, per protein mass | CAT/prot | nmol/min/mg | Hanke, Inken | after Clairborne (1985) | Activity of catalase in liver tissue [nmol (min mg protein)-1] |
| 29 | Superoxide dismutase activity, unit per protein mass | SOD/prot | U/mg | Hanke, Inken | McCord and Fridovich (1969) with adaptation for measurements in microplates described by Lima et al. (2007) | Activity of superoxide dismutase in liver tissue [U (mg protein)-1] |
| 30 | Lipid peroxidation, per wet mass | LPO | nmol/mg | Hanke, Inken | Ohkawa et al. (1979) and Bird and Draper (1984) | Lipid peroxidation in liver tissue, TBARS: thiobarbituric acid reactive substances [nmol TBARS/mg] |
| 31 | Hepatosomatic index | HSI | Hanke, Inken | Calculated | As (liver weight/fish weight) *100 |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
2478 data points
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