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Bausch, Alexandra Renee; Gallego, M Angeles; Harianto, Januar; Thibodeau, Patricia; Bednaršek, Nina; Havenhand, Jonathan N; Klinger, Terrie (2018): Seawater carbonate chemistry and shell dissolution in dead gastropod larvae and adult Limacina helicina pteropods [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.899574, Supplement to: Bausch, AR et al. (2018): Influence of bacteria on shell dissolution in dead gastropod larvae and adult Limacina helicina pteropods under ocean acidification conditions. Marine Biology, 165(2), https://doi.org/10.1007/s00227-018-3293-3

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Abstract:
Ocean acidification (OA) increases aragonite shell dissolution in calcifying marine organisms. It has been proposed that bacteria associated with molluscan shell surfaces in situ could damage the periostracum and reduce its protective function against shell dissolution. However, the influence of bacteria on shell dissolution under OA conditions is unknown. In this study, dissolution in dead shells from gastropod larvae and adult pteropods (Limacina helicina) was examined following a 5-day incubation under a range of aragonite saturation states (Ωarag; values ranging from 0.5 to 1.8) both with and without antibiotics. Gastropod and pteropod specimens were collected from Puget Sound, Washington (48°33′19″N, 122°59′49″W and 47°41′11″N, 122°25′23″W, respectively), preserved, stored, and then treated in August 2015. Environmental scanning electron microscopy (ESEM) was used to determine the severity and extent of dissolution, which was scored as mild, severe, or summed (mild + severe) dissolution. Shell dissolution increased with decreasing Ωarag. In gastropod larvae, there was a significant interaction between the effects of antibiotics and Ωarag on severe dissolution, indicating that microbes could mediate certain types of dissolution among shells under low Ωarag. In L. helicina, there were no significant interactions between the effects of antibiotics and Ωarag on dissolution. These findings suggest that bacteria may differentially influence the response of some groups of shelled planktonic gastropods to OA conditions. This is the first assessment of the microbial–chemical coupling of dissolution in shells of either gastropod larvae or adult L. helicina under OA.
Keyword(s):
Animalia; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Coast and continental shelf; Laboratory experiment; Limacina helicina; Mollusca; North Pacific; Other; Pelagos; Single species; Temperate; Zooplankton
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb
Coverage:
Median Latitude: 48.120835 * Median Longitude: -122.710000 * South-bound Latitude: 47.686390 * West-bound Longitude: -122.996940 * North-bound Latitude: 48.555280 * East-bound Longitude: -122.423060
Date/Time Start: 2014-10-30T00:00:00 * Date/Time End: 2015-08-15T00:00:00
Event(s):
Puget_Sound_OA * Latitude: 47.686390 * Longitude: -122.423060 * Date/Time Start: 2014-10-30T00:00:00 * Date/Time End: 2015-08-15T00:00:00 * Method/Device: Experiment (EXP)
San_Juan_Channel * Latitude: 48.555280 * Longitude: -122.996940 * Date/Time Start: 2014-10-30T00:00:00 * Date/Time End: 2015-08-15T00:00:00 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-03-25.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventBausch, Alexandra Renee
2TypeTypeBausch, Alexandra Reneestudy
3SpeciesSpeciesBausch, Alexandra Renee
4Registration number of speciesReg spec noBausch, Alexandra ReneeWoRMS Aphia ID
5Uniform resource locator/link to referenceURL refBausch, Alexandra Renee
6Experiment durationExp durationdaysBausch, Alexandra Renee
7TreatmentTreatBausch, Alexandra Renee
8ReplicatesRepl#Bausch, Alexandra Renee
9Aragonite saturation stateOmega ArgBausch, Alexandra Renee
10Aragonite saturation state, standard deviationOmega Arg std dev±Bausch, Alexandra Renee
11PercentagePerc%Bausch, Alexandra ReneeMild dissolution
12PercentagePerc%Bausch, Alexandra ReneeSevere dissolution
13PercentagePerc%Bausch, Alexandra ReneeSummed dissolution
14Temperature, waterTemp°CBausch, Alexandra Renee
15Temperature, water, standard deviationTemp std dev±Bausch, Alexandra Renee
16SalinitySalBausch, Alexandra Renee
17Salinity, standard deviationSal std dev±Bausch, Alexandra Renee
18pH, total scalepHTBausch, Alexandra Reneetotal scale
19pH, standard deviationpH std dev±Bausch, Alexandra Reneetotal scale
20Alkalinity, totalATµmol/kgBausch, Alexandra Renee
21Alkalinity, total, standard deviationAT std dev±Bausch, Alexandra Renee
22Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
23Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
24Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
25Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
26Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1754 data points

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