Citation:

s00227-018-3293-3

Name: Seawater carbonate chemistry and shell dissolution in dead gastropod larvae and adult Limacina helicina pteropods
Published: 2018
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Coast and continental shelf; Laboratory experiment; Limacina helicina; Mollusca; North Pacific; Other; Pelagos; Single species; Temperate; Zooplankton

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook TwitterShow Map Google Earth

Abstract:

Ocean acidification (OA) increases aragonite shell dissolution in calcifying marine organisms. It has been proposed that bacteria associated with molluscan shell surfaces in situ could damage the periostracum and reduce its protective function against shell dissolution. However, the influence of bacteria on shell dissolution under OA conditions is unknown. In this study, dissolution in dead shells from gastropod larvae and adult pteropods (Limacina helicina) was examined following a 5-day incubation under a range of aragonite saturation states (Ωarag; values ranging from 0.5 to 1.8) both with and without antibiotics. Gastropod and pteropod specimens were collected from Puget Sound, Washington (48°33′19″N, 122°59′49″W and 47°41′11″N, 122°25′23″W, respectively), preserved, stored, and then treated in August 2015. Environmental scanning electron microscopy (ESEM) was used to determine the severity and extent of dissolution, which was scored as mild, severe, or summed (mild + severe) dissolution. Shell dissolution increased with decreasing Ωarag. In gastropod larvae, there was a significant interaction between the effects of antibiotics and Ωarag on severe dissolution, indicating that microbes could mediate certain types of dissolution among shells under low Ωarag. In L. helicina, there were no significant interactions between the effects of antibiotics and Ωarag on dissolution. These findings suggest that bacteria may differentially influence the response of some groups of shelled planktonic gastropods to OA conditions. This is the first assessment of the microbial–chemical coupling of dissolution in shells of either gastropod larvae or adult L. helicina under OA.

Keyword(s):

Animalia; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Coast and continental shelf; Laboratory experiment; Limacina helicina; Mollusca; North Pacific; Other; Pelagos; Single species; Temperate; Zooplankton

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Median Latitude: 48.120835 * Median Longitude: -122.710000 * South-bound Latitude: 47.686390 * West-bound Longitude: -122.996940 * North-bound Latitude: 48.555280 * East-bound Longitude: -122.423060

Date/Time Start: 2014-10-30T00:00:00 * Date/Time End: 2015-08-15T00:00:00

Event(s):

Puget_Sound_OA * Latitude: 47.686390 * Longitude: -122.423060 * Date/Time Start: 2014-10-30T00:00:00 * Date/Time End: 2015-08-15T00:00:00 * Method/Device: Experiment (EXP)

San_Juan_Channel * Latitude: 48.555280 * Longitude: -122.996940 * Date/Time Start: 2014-10-30T00:00:00 * Date/Time End: 2015-08-15T00:00:00 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-03-25.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Bausch, Alexandra Renee
2	Type	Type		Bausch, Alexandra Renee		study
3	Species	Species		Bausch, Alexandra Renee
4	Registration number of species	Reg spec no		Bausch, Alexandra Renee		WoRMS Aphia ID
5	Uniform resource locator/link to reference	URL ref		Bausch, Alexandra Renee
6	Experiment duration	Exp duration	days	Bausch, Alexandra Renee
7	Treatment	Treat		Bausch, Alexandra Renee
8	Replicates	Repl	#	Bausch, Alexandra Renee
9	Aragonite saturation state	Omega Arg		Bausch, Alexandra Renee
10	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Bausch, Alexandra Renee
11	Percentage	Perc	%	Bausch, Alexandra Renee		Mild dissolution
12	Percentage	Perc	%	Bausch, Alexandra Renee		Severe dissolution
13	Percentage	Perc	%	Bausch, Alexandra Renee		Summed dissolution
14	Temperature, water	Temp	°C	Bausch, Alexandra Renee
15	Temperature, water, standard deviation	Temp std dev	±	Bausch, Alexandra Renee
16	Salinity	Sal		Bausch, Alexandra Renee
17	Salinity, standard deviation	Sal std dev	±	Bausch, Alexandra Renee
18	pH	pH		Bausch, Alexandra Renee		total scale
19	pH, standard deviation	pH std dev	±	Bausch, Alexandra Renee		total scale
20	Alkalinity, total	AT	µmol/kg	Bausch, Alexandra Renee
21	Alkalinity, total, standard deviation	AT std dev	±	Bausch, Alexandra Renee
22	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
23	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
24	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
25	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
26	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
27	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1754 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 Type (study)	3 Species	4 Reg spec no (WoRMS Aphia ID)	5 URL ref	6 Exp duration [days]	7 Treat	8 Repl [#]	9 Omega Arg	10 Omega Arg std dev [±]	11 Perc [%] (Mild dissolution)	12 Perc [%] (Severe dissolution)	13 Perc [%] (Summed dissolution)	14 Temp [°C]	15 Temp std dev [±]	16 Sal	17 Sal std dev [±]	18 pH (total scale)	19 pH std dev [±] (total scale)	20 AT [µmol/kg]	21 AT std dev [±]	22 CSC flag	23 CO2 [µmol/kg]	24 fCO2water_SST_wet [µatm]	25 pCO2water_SST_wet [µatm]	26 [HCO3]- [µmol/kg]	27 [CO3]2- [µmol/kg]	28 DIC [µmol/kg]	29 Omega Arg	30 Omega Cal
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.5 + antibiotics	2	0.558	0.02	1.725	7.611	9.337	14.4	1.0	30.3	0.2	7.44	0.03	2105.47	3.32	8	67.55	1724.43	1730.74	2016.20	35.99	2119.74	0.56	0.88
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.5 + antibiotics	2	0.558	0.02	3.067	7.016	10.083	14.4	1.0	30.3	0.2	7.44	0.03	2105.47	3.32	8	67.55	1724.43	1730.74	2016.20	35.99	2119.74	0.56	0.88
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.6 + antibiotics	2	0.726	0.09	3.040	4.506	7.545	14.3	1.1	30.3	0.2	7.55	0.07	2105.47	3.32	8	51.93	1321.75	1326.59	1992.24	45.64	2089.82	0.71	1.12
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.6 + antibiotics	2	0.726	0.09	5.038	0.000	5.038	14.3	1.1	30.3	0.2	7.55	0.07	2105.47	3.32	8	51.93	1321.75	1326.59	1992.24	45.64	2089.82	0.71	1.12
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.7 + antibiotics	2	0.903	0.12	1.138	0.000	1.138	14.3	1.1	30.4	0.2	7.66	0.05	2105.47	3.32	8	39.65	1009.64	1013.34	1961.44	58.04	2059.13	0.90	1.42
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.7 + antibiotics	2	0.903	0.12	0.684	0.000	0.684	14.3	1.1	30.4	0.2	7.66	0.05	2105.47	3.32	8	39.65	1009.64	1013.34	1961.44	58.04	2059.13	0.90	1.42
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.8 + antibiotics	3	1.206	0.25	0.000	0.297	0.297	14.4	1.2	30.3	0.2	7.79	0.08	2105.47	3.32	8	28.67	731.85	734.52	1915.61	76.56	2020.84	1.19	1.88
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.8 + antibiotics	3	1.206	0.25	0.615	0.000	0.615	14.4	1.2	30.3	0.2	7.79	0.08	2105.47	3.32	8	28.67	731.85	734.52	1915.61	76.56	2020.84	1.19	1.88
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.8 + antibiotics	3	1.206	0.25	0.240	0.000	0.240	14.4	1.2	30.3	0.2	7.79	0.08	2105.47	3.32	8	28.67	731.85	734.52	1915.61	76.56	2020.84	1.19	1.88
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.9 + antibiotics	2	1.254	0.15	1.039	0.000	1.039	14.9	1.2	30.4	0.2	7.78	0.03	2105.47	3.32	8	28.96	751.27	753.99	1915.87	76.48	2021.31	1.19	1.88
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.9 + antibiotics	2	1.254	0.15	0.414	0.000	0.414	14.9	1.2	30.4	0.2	7.78	0.03	2105.47	3.32	8	28.96	751.27	753.99	1915.87	76.48	2021.31	1.19	1.88
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=8.0 + antibiotics	2	1.597	0.16	0.175	0.000	0.175	14.5	1.2	30.4	0.2	7.93	0.05	2105.47	3.32	8	19.99	512.31	514.18	1850.66	102.75	1973.41	1.60	2.52
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=8.0 + antibiotics	2	1.597	0.16	0.000	0.000	0.000	14.5	1.2	30.4	0.2	7.93	0.05	2105.47	3.32	8	19.99	512.31	514.18	1850.66	102.75	1973.41	1.60	2.52
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.5	1	0.561	0.03	7.918	0.698	8.616	14.5	1.1	30.3	0.2	7.44	0.04	2105.47	3.32	8	67.38	1725.38	1731.68	2015.88	36.13	2119.38	0.56	0.89
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.6	2	0.860	0.12	14.084	0.208	14.292	14.5	1.2	30.3	0.2	7.61	0.09	2105.47	3.32	8	44.64	1143.19	1147.36	1975.60	52.37	2072.61	0.82	1.29
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.6	2	0.860	0.12	5.818	0.000	5.818	14.5	1.2	30.3	0.2	7.61	0.09	2105.47	3.32	8	44.64	1143.19	1147.36	1975.60	52.37	2072.61	0.82	1.29
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.7	2	1.050	0.25	5.330	0.000	5.330	14.2	1.0	30.4	0.2	7.72	0.09	2105.47	3.32	8	34.28	870.18	873.37	1942.33	65.73	2042.34	1.02	1.61
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.7	2	1.050	0.25	3.812	0.000	3.812	14.2	1.0	30.4	0.2	7.72	0.09	2105.47	3.32	8	34.28	870.18	873.37	1942.33	65.73	2042.34	1.02	1.61
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.8	1	1.315	0.29	1.480	0.000	1.480	14.3	1.1	30.3	0.2	7.83	0.09	2105.47	3.32	8	25.99	661.47	663.89	1899.80	82.93	2008.72	1.29	2.04
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=7.9	1	1.262	0.09	4.829	0.000	4.829	14.3	1.0	30.4	0.2	7.82	0.04	2105.47	3.32	8	26.62	677.86	680.34	1903.48	81.41	2011.51	1.27	2.00
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=8	2	1.786	0.15	0.000	0.000	0.000	14.3	1.0	30.4	0.2	7.99	0.04	2105.47	3.32	8	17.21	438.18	439.78	1819.94	115.13	1952.28	1.79	2.82
San_Juan_Channel	laboratory	Gastropod larvae			5	pH=8	2	1.786	0.15	0.589	0.000	0.589	14.3	1.0	30.4	0.2	7.99	0.04	2105.47	3.32	8	17.21	438.18	439.78	1819.94	115.13	1952.28	1.79	2.82
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.5 + antibiotics	3	0.498	0.03	17.781	6.666	24.447	14.6	1.1	30.3	0.2	7.38	0.03	2105.47	3.32	8	77.59	1993.07	2000.35	2026.74	31.76	2136.09	0.50	0.78
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.5 + antibiotics	3	0.498	0.03	12.799	20.191	32.990	14.6	1.1	30.3	0.2	7.38	0.03	2105.47	3.32	8	77.59	1993.07	2000.35	2026.74	31.76	2136.09	0.50	0.78
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.5 + antibiotics	3	0.498	0.03	15.025	14.161	29.185	14.6	1.1	30.3	0.2	7.38	0.03	2105.47	3.32	8	77.59	1993.07	2000.35	2026.74	31.76	2136.09	0.50	0.78
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.6 + antibiotics	2	0.717	0.09	1.537	1.206	2.743	14.5	1.2	30.3	0.2	7.55	0.08	2105.47	3.32	8	51.67	1323.08	1327.92	1991.45	45.98	2089.09	0.72	1.13
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.6 + antibiotics	2	0.717	0.09	3.845	0.031	3.876	14.5	1.2	30.3	0.2	7.55	0.08	2105.47	3.32	8	51.67	1323.08	1327.92	1991.45	45.98	2089.09	0.72	1.13
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.7 + antibiotics	4	0.872	0.27	0.069	0.000	0.069	14.6	1.2	30.4	0.2	7.62	0.12	2105.47	3.32	8	43.40	1115.33	1119.40	1971.93	53.83	2069.16	0.84	1.32
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.7 + antibiotics	4	0.872	0.27	0.032	0.000	0.032	14.6	1.2	30.4	0.2	7.62	0.12	2105.47	3.32	8	43.40	1115.33	1119.40	1971.93	53.83	2069.16	0.84	1.32
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.7 + antibiotics	4	0.872	0.27	0.198	0.000	0.198	14.6	1.2	30.4	0.2	7.62	0.12	2105.47	3.32	8	43.40	1115.33	1119.40	1971.93	53.83	2069.16	0.84	1.32
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.7 + antibiotics	4	0.872	0.27	0.397	0.000	0.397	14.6	1.2	30.4	0.2	7.62	0.12	2105.47	3.32	8	43.40	1115.33	1119.40	1971.93	53.83	2069.16	0.84	1.32
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.8 + antibiotics	4	1.196	0.24	0.157	0.000	0.157	14.2	0.9	30.3	0.2	7.79	0.08	2105.47	3.32	8	28.82	731.30	733.98	1916.89	76.02	2021.74	1.18	1.87
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.8 + antibiotics	4	1.196	0.24	0.078	0.000	0.078	14.2	0.9	30.3	0.2	7.79	0.08	2105.47	3.32	8	28.82	731.30	733.98	1916.89	76.02	2021.74	1.18	1.87
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.8 + antibiotics	4	1.196	0.24	0.000	0.000	0.000	14.2	0.9	30.3	0.2	7.79	0.08	2105.47	3.32	8	28.82	731.30	733.98	1916.89	76.02	2021.74	1.18	1.87
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.8 + antibiotics	4	1.196	0.24	0.133	0.000	0.133	14.2	0.9	30.3	0.2	7.79	0.08	2105.47	3.32	8	28.82	731.30	733.98	1916.89	76.02	2021.74	1.18	1.87
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.9 + antibiotics	3	1.127	0.09	0.027	0.000	0.027	14.5	1.1	30.4	0.2	7.76	0.04	2105.47	3.32	8	30.78	788.66	791.53	1926.11	72.30	2029.20	1.13	1.77
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.9 + antibiotics	3	1.127	0.09	0.027	0.000	0.027	14.5	1.1	30.4	0.2	7.76	0.04	2105.47	3.32	8	30.78	788.66	791.53	1926.11	72.30	2029.20	1.13	1.77
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.9 + antibiotics	3	1.127	0.09	0.077	0.000	0.077	14.5	1.1	30.4	0.2	7.76	0.04	2105.47	3.32	8	30.78	788.66	791.53	1926.11	72.30	2029.20	1.13	1.77
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=8.0 + antibiotics	4	1.601	0.14	0.020	0.000	0.020	14.6	1.1	30.4	0.2	7.93	0.04	2105.47	3.32	8	19.94	512.44	514.31	1849.83	103.10	1972.87	1.61	2.53
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=8.0 + antibiotics	4	1.601	0.14	0.170	0.000	0.170	14.6	1.1	30.4	0.2	7.93	0.04	2105.47	3.32	8	19.94	512.44	514.31	1849.83	103.10	1972.87	1.61	2.53
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=8.0 + antibiotics	4	1.601	0.14	0.012	0.000	0.012	14.6	1.1	30.4	0.2	7.93	0.04	2105.47	3.32	8	19.94	512.44	514.31	1849.83	103.10	1972.87	1.61	2.53
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=8.0 + antibiotics	4	1.601	0.14	0.183	0.000	0.183	14.6	1.1	30.4	0.2	7.93	0.04	2105.47	3.32	8	19.94	512.44	514.31	1849.83	103.10	1972.87	1.61	2.53
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.5	3	0.574	0.05	14.202	4.365	18.566	14.3	1.1	30.3	0.2	7.45	0.04	2105.47	3.32	8	66.11	1682.59	1688.76	2014.53	36.66	2117.30	0.57	0.90
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.5	3	0.574	0.05	18.381	2.544	20.925	14.3	1.1	30.3	0.2	7.45	0.04	2105.47	3.32	8	66.11	1682.59	1688.76	2014.53	36.66	2117.30	0.57	0.90
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.5	3	0.574	0.05	20.598	6.438	27.036	14.3	1.1	30.3	0.2	7.45	0.04	2105.47	3.32	8	66.11	1682.59	1688.76	2014.53	36.66	2117.30	0.57	0.90
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.6	2	0.778	0.13	0.219	0.106	0.325	14.2	1.0	30.3	0.2	7.59	0.10	2105.47	3.32	8	47.24	1198.64	1203.03	1982.39	49.61	2079.24	0.77	1.22
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.6	2	0.778	0.13	0.687	0.000	0.687	14.2	1.0	30.3	0.2	7.59	0.10	2105.47	3.32	8	47.24	1198.64	1203.03	1982.39	49.61	2079.24	0.77	1.22
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.7	4	0.991	0.20	0.330	0.000	0.330	14.6	0.9	30.4	0.2	7.69	0.08	2105.47	3.32	8	36.53	938.89	942.31	1950.31	62.55	2049.39	0.97	1.54
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.7	4	0.991	0.20	0.252	0.000	0.252	14.6	0.9	30.4	0.2	7.69	0.08	2105.47	3.32	8	36.53	938.89	942.31	1950.31	62.55	2049.39	0.97	1.54
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.7	4	0.991	0.20	0.153	0.000	0.153	14.6	0.9	30.4	0.2	7.69	0.08	2105.47	3.32	8	36.53	938.89	942.31	1950.31	62.55	2049.39	0.97	1.54
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.7	4	0.991	0.20	0.184	0.000	0.184	14.6	0.9	30.4	0.2	7.69	0.08	2105.47	3.32	8	36.53	938.89	942.31	1950.31	62.55	2049.39	0.97	1.54
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.8	5	1.190	0.14	0.131	0.000	0.131	14.7	1.1	30.3	0.2	7.80	0.09	2105.47	3.32	8	27.73	714.46	717.06	1909.63	79.01	2016.37	1.23	1.94
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.8	5	1.190	0.14	0.063	0.000	0.063	14.7	1.1	30.3	0.2	7.80	0.09	2105.47	3.32	8	27.73	714.46	717.06	1909.63	79.01	2016.37	1.23	1.94
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.8	5	1.190	0.14	0.060	0.000	0.060	14.7	1.1	30.3	0.2	7.80	0.09	2105.47	3.32	8	27.73	714.46	717.06	1909.63	79.01	2016.37	1.23	1.94
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.8	5	1.190	0.14	0.034	0.000	0.034	14.7	1.1	30.3	0.2	7.80	0.09	2105.47	3.32	8	27.73	714.46	717.06	1909.63	79.01	2016.37	1.23	1.94
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.8	5	1.190	0.14	0.085	0.000	0.085	14.7	1.1	30.3	0.2	7.80	0.09	2105.47	3.32	8	27.73	714.46	717.06	1909.63	79.01	2016.37	1.23	1.94
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.9	3	1.159	0.10	0.000	0.000	0.000	14.5	1.2	30.4	0.2	7.77	0.03	2105.47	3.32	8	30.02	769.18	771.99	1922.30	73.84	2026.16	1.15	1.81
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.9	3	1.159	0.10	0.000	0.038	0.038	14.5	1.2	30.4	0.2	7.77	0.03	2105.47	3.32	8	30.02	769.18	771.99	1922.30	73.84	2026.16	1.15	1.81
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=7.9	3	1.159	0.10	0.346	0.000	0.346	14.5	1.2	30.4	0.2	7.77	0.03	2105.47	3.32	8	30.02	769.18	771.99	1922.30	73.84	2026.16	1.15	1.81
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=8	3	1.762	0.16	0.011	0.000	0.011	14.5	1.2	30.4	0.2	7.97	0.04	2105.47	3.32	8	18.02	461.85	463.53	1829.32	111.37	1958.72	1.74	2.73
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=8	3	1.762	0.16	0.051	0.000	0.051	14.5	1.2	30.4	0.2	7.97	0.04	2105.47	3.32	8	18.02	461.85	463.53	1829.32	111.37	1958.72	1.74	2.73
Puget_Sound_OA	laboratory	Limacina helicina (zooplankton)	140223	marinespecies.org	5	pH=8	3	1.762	0.16	0.000	0.000	0.000	14.5	1.2	30.4	0.2	7.97	0.04	2105.47	3.32	8	18.02	461.85	463.53	1829.32	111.37	1958.72	1.74	2.73