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Schreiber, L; Holler, L; Knittel, Katrin; Meyerdierks, Anke; Amann, Rudolf (2016): Anaerobic methanotrophic archaea-2 and Desulfosarcina/Desulfococcus group in sediments at different stations. PANGAEA, (unpublished dataset), Supplement to: Schreiber, L et al. (2010): Identification of the dominant sulfate-reducing bacterial partner of anaerobic methanotrophs of the ANME-2 clade. Environmental Microbiology, 12(8), 2327-2340,

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The anaerobic oxidation of methane (AOM) with sulfate as terminal electron acceptor is mediated by consortia of methanotrophic archaea (ANME) and sulfate-reducing bacteria (SRB). In sediment samples from Hydrate Ridge, the Isis Mud Volcano and the Gulf of Mexico, DSS cells accounted for 3-6% of all DAPI-stained single cells. Out of these, 8-17% were labelled with probe SEEP1a-1441. This translated into relative abundances of single SEEP-SRB1a cells of 0.3% to 0.7%. Contrastingly, in a sediment sample from the Gullfaks oil field, DSS cells accounted for 18% and SEEP-SRB1a for 9% of all single cells. This sediment sample also featured an unusually high abundance of single ANME-2 cells and only very few ANME-2/DSS aggregates in comparison with other AOM habitats. Considering also the nature of the sample, it is likely that the high number of single ANME-2 and SEEP-SRB1a cells were an artifact of sample preparation. Here, harsher sonication was required to remove the microorganisms from coarse sand prior to CARD-FISH analysis.
Source data set:
Knittel, Katrin; Lösekann, Tina; Boetius, Antje; Kort, Renate; Amann, Rudolf (2016): Relative abundance of anaerobic methanotrophic archaea in bacterial mat Cascadia margin off Oregon. PANGAEA,
Wegener, Gunter; Shovitri, M; Knittel, Katrin; Niemann, H; Hovland, Martin; Boetius, Antje (2016): Microbial community in sediment of Gullfaks and Tommeliten methane seeps in the Northern North Sea. PANGAEA,
Median Latitude: 42.799431 * Median Longitude: -79.780501 * South-bound Latitude: 27.782460 * West-bound Longitude: -125.147450 * North-bound Latitude: 61.174335 * East-bound Longitude: 31.389750
Date/Time Start: 2000-07-27T04:40:00 * Date/Time End: 2006-11-14T04:00:00
Minimum Elevation: -992.0 m * Maximum Elevation: -145.0 m
HE208/771 * Latitude: 61.174335 * Longitude: 2.242584 * Date/Time: 2004-05-17T11:53:10 * Elevation: -145.0 m * Recovery: 0.2 m * Location: Kvitebjorn * Campaign: HE208 * Basis: Heincke * Method/Device: Multicorer with television (TVMUC)
M70/2b_812_PUC-8 (812-8) * Latitude: 32.361070 * Longitude: 31.389750 * Date/Time: 2006-11-14T04:00:00 * Elevation: -992.0 m * Location: Isis Mud Volcano * Campaign: M70/2b (BIONIL) * Basis: Meteor (1986) * Method/Device: Push corer (PUC) * Comment: core 8 taken again again heavy ebullition
SO148/1_19-2 (TV-MUC-6) * Latitude Start: 44.569950 * Longitude Start: -125.146550 * Latitude End: 44.568400 * Longitude End: -125.146783 * Date/Time Start: 2000-07-27T04:40:00 * Date/Time End: 2000-07-27T07:02:00 * Elevation: -777.0 m * Recovery: 0.26 m * Campaign: SO148/1 (TECFLUX II) * Basis: Sonne * Method/Device: Multicorer with television (TVMUC) * Comment: 6 cores, 20-26 cm
Total and absolute cell numbers based on Knittel et al. (2005; doi:10.1128/AEM.71.1.467-479.2005) and Wegener et al. (2008; doi:10.5194/bg-5-1127-2008).
30 data points

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