Giannakourou, Antonia; Pitta, Paraskevi: Bacterial abundance in the eastern Mediteranean Sea in August and September 2008 during SES_GR2 [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.853925 (unpublished dataset)
Abstract:
The SES_GR2_MICROBIAL PARAMETERS dataset is based on samples collected in the framework of the project SESAME, in the Ionian, Libyan and Aegean Sea during August-September 2008. The objectives were to measure the standing stocks and calculate the production of the microbial compartment of the food web, describe the vertical distribution pattern and characterize its structure and function through the water column. Subsamples for virus, heterotrophic bacteria and cyanobacteria (Synechococcus spp. and Prochlorococcus spp.) counting were analyzed using a FACSCalibur (Becton Dickinson) flow cytometer equipped with a standard laser (488 nm) and filter set and using deionized water as sheath fluid. Fluorescent beads with a diameter of 0.97 μm (Polysciences) were added to each sample as an internal standard, and all parameters were normalized to the beads and expressed as relative units. SYBRGreen I stain (Molecular Probe) was used to stain viral and heterotrophic bacterial DNA. Viruses were counted according to (Brussaard 1984). In order to avoid bulk consentrations of viruses samples we dilluted to Tris-EDTA (pH=8,0) buffer to a final sollution of 1/5 to 1/100. Total abundance and nucleid content classes were calculated using the Paint-A-Gate software (Becton Dickinson).
Related to:
Giannakourou, Antonia; Pitta, Paraskevi: Bacterial production in the eastern Mediteranean Sea in August and September 2008 during SES_GR2 [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.853926
Further details:
Brussaard, Corina P D (2004): Optimization of Procedures for Counting Viruses by Flow Cytometry. Applied and Environmental Microbiology, 70(3), 1506-1513, https://doi.org/10.1128/AEM.70.3.1506-1513.2004
Project(s):
Funding:
Sixth Framework Programme (FP6), grant/award no. 36949: Southern European Seas: Assessing and Modelling Ecosystem Changes
Coverage:
Median Latitude: 36.514018 * Median Longitude: 23.694133 * South-bound Latitude: 34.377170 * West-bound Longitude: 18.000330 * North-bound Latitude: 40.267170 * East-bound Longitude: 26.234000
Date/Time Start: 2008-08-28T03:55:00 * Date/Time End: 2008-09-07T09:10:00
Minimum DEPTH, water: 2 m * Maximum DEPTH, water: 4000 m
Event(s):
SES_GR2-00A1d_NIS (00A1d) * Latitude: 40.267170 * Longitude: 25.239000 * Date/Time: 2008-09-07T09:10:00 * Location: East Mediterranean Sea * Campaign: SES_GR2 * Basis: Aegaeo * Method/Device: Monegasque Trawl (MTRW) * Comment: All bottles fired. All bottles were checked before and after they were fired.
SES_GR2-00A02_NIS (00A02) * Latitude: 39.777500 * Longitude: 25.518670 * Date/Time: 2008-09-06T15:10:00 * Location: Eastern Mediterranean Sea * Campaign: SES_GR2 * Basis: Aegaeo * Method/Device: Bottle, Niskin (NIS) * Comment: All bottles fired. All bottles were checked before and after they were fired.
SES_GR2-0A03c_NIS (0A03c) * Latitude: 39.219500 * Longitude: 24.988170 * Date/Time: 2008-09-06T09:45:00 * Location: East Mediterranean Sea * Campaign: SES_GR2 * Basis: Aegaeo * Method/Device: Monegasque Trawl (MTRW) * Comment: All bottles fired. All bottles were checked before and after they were fired.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Zervoudaki, Soultana | |||
| 2 | Optional event label | Event 2 | Zervoudaki, Soultana | |||
| 3 | Date/Time of event | Date/Time | Zervoudaki, Soultana | |||
| 4 | Latitude of event | Latitude | Zervoudaki, Soultana | |||
| 5 | Longitude of event | Longitude | Zervoudaki, Soultana | |||
| 6 | DEPTH, water | Depth water | m | Zervoudaki, Soultana | Geocode | |
| 7 | Bacteria, heterotrophic | HBA | #/ml | Zervoudaki, Soultana | Measured/Determined | |
| 8 | Prochlorococcus | Prochlorococcus | #/ml | Zervoudaki, Soultana | Measured/Determined | |
| 9 | Synechococcus | Synechococcus | #/ml | Zervoudaki, Soultana | Measured/Determined | |
| 10 | Viral abundance | Virus | #/ml | Zervoudaki, Soultana | Measured/Determined |
Size:
628 data points