Data Description

Citation:
Kreiss, CM et al. (2015): Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua). doi:10.1594/PANGAEA.848599,
Supplement to: Kreiss, Cornelia M; Michael, Katharina; Bock, Christian; Lucassen, Magnus; Pörtner, Hans-Otto (2015): Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills of Atlantic cod (Gadus morhua). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 182, 102-112, doi:10.1016/j.cbpa.2014.12.019
Abstract:
Effects of severe hypercapnia have been extensively studied in marine fishes, while knowledge on the impacts of moderately elevated CO2 levels and their combination with warming is scarce. Here we investigate ion regulation mechanisms and energy budget in gills from Atlantic cod acclimated long-term to elevated PCO2 levels (2500 µatm) and temperature (18 °C). Isolated perfused gill preparations established to determine gill thermal plasticity during acute exposures (10-22 °C) and in vivo costs of Na+/K+-ATPase activity, protein and RNA synthesis. Maximum enzyme capacities of F1Fo-ATPase, H+-ATPase and Na+/K+-ATPase were measured in vitro in crude gill homogenates. After whole animal acclimation to elevated PCO2 and/or warming, branchial oxygen consumption responded more strongly to acute temperature change. The fractions of gill respiration allocated to protein and RNA synthesis remained unchanged. In gills of fish CO2-exposed at both temperatures, energy turnover associated with Na+/K+-ATPase activity was reduced by 30% below rates of control fish. This contrasted in vitro capacities of Na+/K+-ATPase, which remained unchanged under elevated CO2 at 10 °C, and earlier studies which had found a strong upregulation under severe hypercapnia. F1Fo-ATPase capacities increased in hypercapnic gills at both temperatures, whereas Na+/K+ATPase and H+-ATPase capacities only increased in response to elevated CO2 and warming indicating the absence of thermal compensation under CO2. We conclude that in vivo ion regulatory energy demand is lowered under moderately elevated CO2 levels despite the stronger thermal response of total gill respiration and the upregulation of F1Fo-ATPase. This effect is maintained at elevated temperature.
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloise (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb *
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-08-06
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Species *SpeciesKreiss, Cornelia M *
2Figure *FigKreiss, Cornelia M *
3Tissues *TissuesKreiss, Cornelia M *gill tissue
4Concentration *Concµmol/lKreiss, Cornelia M *Metabolite concentrations (succinate µM)
5Concentration *Concµmol/lKreiss, Cornelia M *Metabolite concentrations (lactate µM)
6Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmKreiss, Cornelia M *Treatment
7Treatment: temperature *T:temp°CKreiss, Cornelia M *incubation temperature
8Treatment: temperature *T:temp°CKreiss, Cornelia M *acute experimental temperature
9Respiration rate, oxygen *Resp O2µmol/g/hKreiss, Cornelia M *gill respiration MO2
10Respiration rate, oxygen, standard deviation *Resp O2 std dev±Kreiss, Cornelia M *Calculated *gill respiration MO2
11Description *DescriptionKreiss, Cornelia M *inhibited process
12Respiration rate, oxygen *Resp O2µmol/g/hKreiss, Cornelia M *MO2
13Respiration rate, oxygen, standard deviation *Resp O2 std dev±Kreiss, Cornelia M *Calculated *MO2
14Fraction *Frac%Kreiss, Cornelia M *fraction of gill respiration
15Standard deviation *Std dev±Kreiss, Cornelia M *fraction of gill respiration
16Enzyme *EnzymeKreiss, Cornelia M *
17Adenosine triphosphatase activity *ATPase actµmol/g/hKreiss, Cornelia M *enzyme capacity
18Standard deviation *Std dev±Kreiss, Cornelia M *enzyme capacity
19Adenosine triphosphatase activity *ATPase actµmol/g/hKreiss, Cornelia M *F1Fo-ATPase
20Sodium/Potassium adenosine triphosphatase activity *Na/K-ATPase actµmol/g/hKreiss, Cornelia M *
21Adenosine triphosphatase activity *ATPase actµmol/g/hKreiss, Cornelia M *H+-ATPase
22Temperature, water *Temp°CKreiss, Cornelia M *CTD, SEA-BIRD SBE 911plus *
23Temperature, water, standard deviation *Temp std dev±Kreiss, Cornelia M *Temperature
24Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmKreiss, Cornelia M *
25Partial pressure of carbon dioxide, standard deviation *pCO2 std dev±Kreiss, Cornelia M *
26pH *pHKreiss, Cornelia M *NBS scale
27pH, standard deviation *pH std dev±Kreiss, Cornelia M *NBS scale
28pH *pHKreiss, Cornelia M *total scale
29pH, standard deviation *pH std dev±Kreiss, Cornelia M *total scale
30Salinity *SalKreiss, Cornelia M *
31Salinity, standard deviation *Sal std dev±Kreiss, Cornelia M *
32Carbon, inorganic, dissolved *DICµmol/lKreiss, Cornelia M *
33Carbon, inorganic, dissolved, standard deviation *DIC std dev±Kreiss, Cornelia M *
34Carbonate system computation flag *CSC flagYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
35pH *pHYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *total scale
36Carbon dioxide *CO2µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
37Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
38Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
39Bicarbonate ion *[HCO3]-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
40Carbonate ion *[CO3]2-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
41Carbon, inorganic, dissolved *DICµmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
42Alkalinity, total *ATµmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
43Aragonite saturation state *Omega ArgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
44Calcite saturation state *Omega CalYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
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