Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

Horwitz, Rael; Borell, Esther M; Yam, Ruth; Shemesh, Aldo; Fine, Maoz (2015): Natural high pCO2 increases autotrophy in Anemonia viridis (Anthozoa) as revealed from stable isotope (C, N) analysis [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.846688, Supplement to: Horwitz, R et al. (2015): Natural high pCO2 increases autotrophy in Anemonia viridis (Anthozoa) as revealed from stable isotope (C, N) analysis. Scientific Reports, 5, 8779, https://doi.org/10.1038/srep08779

Always quote citation above when using data! You can download the citation in several formats below.

RIS CitationBibTeX CitationShow MapGoogle Earth

Abstract:
Contemporary cnidarian-algae symbioses are challenged by increasing CO2 concentrations (ocean warming and acidification) affecting organisms' biological performance. We examined the natural variability of carbon and nitrogen isotopes in the symbiotic sea anemone Anemonia viridis to investigate dietary shifts (autotrophy/heterotrophy) along a natural pCO2 gradient at the island of Vulcano, Italy. delta 13C values for both algal symbionts (Symbiodinium) and host tissue of A. viridis became significantly lighter with increasing seawater pCO2. Together with a decrease in the difference between delta 13C values of both fractions at the higher pCO2 sites, these results indicate there is a greater net autotrophic input to the A. viridis carbon budget under high pCO2 conditions. delta 15N values and C/N ratios did not change in Symbiodinium and host tissue along the pCO2 gradient. Additional physiological parameters revealed anemone protein and Symbiodinium chlorophyll a remained unaltered among sites. Symbiodinium density was similar among sites yet their mitotic index increased in anemones under elevated pCO2. Overall, our findings show that A. viridis is characterized by a higher autotrophic/heterotrophic ratio as pCO2 increases. The unique trophic flexibility of this species may give it a competitive advantage and enable its potential acclimation and ecological success in the future under increased ocean acidification.
Keyword(s):
Anemonia viridis; Animalia; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Cnidaria; CO2 vent; Coast and continental shelf; Field observation; Mediterranean Sea; Single species; Symbiodinium sp.; Temperate
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Coverage:
Latitude: 38.416670 * Longitude: 14.950000
Date/Time Start: 2012-05-01T00:00:00 * Date/Time End: 2012-05-31T00:00:00
Event(s):
Levante_Bay_OA * Latitude: 38.416670 * Longitude: 14.950000 * Date/Time Start: 2012-05-01T00:00:00 * Date/Time End: 2012-05-31T00:00:00 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-06-01.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesHorwitz, Rael
2FigureFigHorwitz, Rael
3SiteSiteHorwitz, Rael
4Sample IDSample IDHorwitz, Rael
5Absolute protein contentProteinmgHorwitz, Rael
6Wet massWet mgHorwitz, Rael
7Proteins, totalTPRTµg/gHorwitz, Rael
8Symbiont cell densitySymbiont#/mgHorwitz, Raelper protein
9Chlorophyll a per cellChl a/cellpg/#Horwitz, Rael
10Mitotic indexMitotic indexHorwitz, Rael
11δ13Cδ13C‰ PDBHorwitz, Raelseawater
12δ18Oδ18O‰Horwitz, Raelseawater
13δ13Cδ13C‰ PDBHorwitz, Raeltissue
14δ13Cδ13C‰ PDBHorwitz, Raelsymbiodinium
15δ15Nδ15N‰ airHorwitz, Raeltissue
16δ15Nδ15N‰ airHorwitz, Raelsymbiodinium
17Carbon/Nitrogen ratioC/NHorwitz, Raeltissue
18Carbon/Nitrogen ratioC/NHorwitz, Raelsymbiodinium
19SalinitySalHorwitz, Rael
20pHpHHorwitz, RaelPotentiometricNBS scale
21pH, standard deviationpH std dev±Horwitz, RaelPotentiometricNBS scale
22Temperature, waterTemp°CHorwitz, Rael
23Temperature, water, standard deviationTemp std dev±Horwitz, Rael
24Alkalinity, totalATµmol/kgHorwitz, RaelPotentiometric titration
25Alkalinity, total, standard deviationAT std dev±Horwitz, RaelPotentiometric titration
26Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmHorwitz, RaelCalculated using CO2SYS
27Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Horwitz, RaelCalculated using CO2SYS
28Carbon, inorganic, dissolvedDICµmol/kgHorwitz, RaelCalculated using CO2SYS
29Carbon, inorganic, dissolved, standard deviationDIC std dev±Horwitz, RaelCalculated using CO2SYS
30Bicarbonate ion[HCO3]-µmol/kgHorwitz, RaelCalculated using CO2SYS
31Bicarbonate ion, standard deviation[HCO3]- std dev±Horwitz, RaelCalculated using CO2SYS
32Carbonate ion[CO3]2-µmol/kgHorwitz, RaelCalculated using CO2SYS
33Carbonate ion, standard deviation[CO3]2- std dev±Horwitz, RaelCalculated using CO2SYS
34Carbon dioxideCO2µmol/kgHorwitz, RaelCalculated using CO2SYS
35Carbon dioxide, standard deviationCO2 std dev±Horwitz, RaelCalculated using CO2SYS
36Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
37pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
38Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
39Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
40Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
41Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
42Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
43Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
44Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
45Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
7065 data points

Download Data

Download dataset as tab-delimited text — use the following character encoding:

View dataset as HTML (shows only first 2000 rows)