Citation:

PANGAEA.914234

Name: Seawater carbonate chemistry and photosynthetic potential, cell density, lipid content of Symbiodinium
Published: 2019
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Myzozoa; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; South Atlantic; Tropical

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook Twitter

Abstract:

Dinoflagellates from the Symbiodiniaceae family and corals have an ecologically important endosymbiotic relationship. Scleractinian corals cannot survive for long periods without their symbionts. These algae, also known as zooxanthellae, on the other hand, thrives outside the coral cells. The free-living populations of zooxanthellae are essential for the resilience of the coral to environmental stressors such as temperature anomalies and ocean acidification. Yet, little is known about how ocean acidification may affect the free-living zooxanthellae. In this study we aimed to test morphological, physiological and biochemical responses of zooxanthellae from the Symbiodinium genus isolated from the coral Mussismilia braziliensis, endemic to the Brazilian coast, to acidification led by increased atmospheric CO2. We tested whether photosynthetic yield, cell ultrastructure, cell density and lipid profile would change after up to 16 days of exposure to pH 7.5 in an atmospheric pCO2 of 1633 μatm. Photosynthetic yield and cell density were negatively affected and chloroplasts showed vesiculated thylakoids, indicating morphological damage. Moreover, Symbiodinium fatty acid profile drastically changed in acidified condition, showing lower polyunsaturated fatty acids and higher saturated fatty acids contents, when compared to the control, non-acidified condition. These results show that seawater acidification as an only stressor causes significant changes in the physiology, biochemistry and ultrastructure of free-living Symbiodinium.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Myzozoa; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; South Atlantic; Tropical

Supplement to:

Hill, Lilian J; Paradas, Wladimir C; Willemes, Maria Julia; Pereira, Miria G; Salomon, Paulo S; Mariath, Rodrigo; Moura, Rodrigo L; Atella, Georgia C; Farina, Marcos; Amado-Filho, Gilberto M; Salgado, Leonardo T (2019): Acidification-induced cellular changes in Symbiodinium isolated from Mussismilia braziliensis. PLoS ONE, 14(8), e0220130, https://doi.org/10.1371/journal.pone.0220130

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-03-18.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Salgado, Leonardo T		study
2	Species	Species		Salgado, Leonardo T
3	Treatment	Treat		Salgado, Leonardo T
4	Experiment day	Exp day	day	Salgado, Leonardo T
5	Maximum photochemical quantum yield of photosystem II	Fv/Fm		Salgado, Leonardo T
6	Photochemical quantum yield	Fv/Fm		Salgado, Leonardo T
7	Number of cells	No cells	#	Salgado, Leonardo T
8	Growth rate	µ	1/day	Salgado, Leonardo T
9	Lipids	Lipids		Salgado, Leonardo T		Sterols species
10	Percentage	Perc	%	Salgado, Leonardo T		Sterols species
11	Lipids	Lipids		Salgado, Leonardo T		FAs classes
12	Percentage	Perc	%	Salgado, Leonardo T		FAs classes
13	Number of pixels	No pix	#	Salgado, Leonardo T
14	Partial pressure of carbon dioxide (ambient atmosphere)	pCO2air	µatm	Salgado, Leonardo T
15	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Salgado, Leonardo T
16	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Salgado, Leonardo T
17	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Salgado, Leonardo T
18	Carbon dioxide	CO2	µmol/kg	Salgado, Leonardo T
19	Carbon dioxide, standard deviation	CO2 std dev	±	Salgado, Leonardo T
20	pH	pH		Salgado, Leonardo T		total scale
21	pH, standard deviation	pH std dev	±	Salgado, Leonardo T		total scale
22	Bicarbonate ion	[HCO3]-	µmol/kg	Salgado, Leonardo T
23	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Salgado, Leonardo T
24	Carbonate ion	[CO3]2-	µmol/kg	Salgado, Leonardo T
25	Carbonate ion, standard deviation	[CO3]2- std dev	±	Salgado, Leonardo T
26	Aragonite saturation state	Omega Arg		Salgado, Leonardo T
27	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Salgado, Leonardo T
28	Calcite saturation state	Omega Cal		Salgado, Leonardo T
29	Calcite saturation state, standard deviation	Omega Cal std dev	±	Salgado, Leonardo T
30	Alkalinity, total	AT	µmol/kg	Salgado, Leonardo T
31	Alkalinity, total, standard deviation	AT std dev	±	Salgado, Leonardo T
32	Temperature, water	Temp	°C	Salgado, Leonardo T
33	Temperature, water, standard deviation	Temp std dev	±	Salgado, Leonardo T
34	Salinity	Sal		Salgado, Leonardo T
35	Salinity, standard deviation	Sal std dev	±	Salgado, Leonardo T
36	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

6995 data points

Download Data

Download dataset as tab-delimited text — use the following character encoding:

View dataset as HTML (shows only first 2000 rows)