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Cumbo, Vivian R; Fan, Tung-Yung; Edmunds, Peter J (2012): Seawater carbonate chemistry and protein content, respiration, symbiodinium densities, survivorship of Pocillopora damicornis larvae in a laboratory experiment [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.823582, Supplement to: Cumbo, VR et al. (2013): Effects of exposure duration on the response of Pocillopora damicornis larvae to elevated temperature and high pCO2. Journal of Experimental Marine Biology and Ecology, 439, 100-107, https://doi.org/10.1016/j.jembe.2012.10.019

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Abstract:
Efforts to evaluate the response of coral larvae to global climate change (GCC) and ocean acidification (OA) typically employ short experiments of fixed length, yet it is unknown how the response is affected by exposure duration. In this study, we exposed larvae from the brooding coral Pocillopora damicornis to contrasts of temperature (24.00 °C [ambient] versus 30.49 °C) and pCO2 (49.4 Pa versus 86.2 Pa) for varying periods (1-5 days) to test the hypothesis that exposure duration had no effect on larval response as assessed by protein content, respiration, Symbiodinium density, and survivorship; exposure times were ecologically relevant compared to representative pelagic larval durations (PLD) for corals. Larvae differed among days for all response variables, and the effects of the treatment were relatively consistent regardless of exposure duration for three of the four response variables. Protein content and Symbiodinium density were unaffected by temperature and pCO2, but respiration increased with temperature (but not pCO2) with the effect intensifying as incubations lengthened. Survival, however, differed significantly among treatments at the end of the study, and by the 5th day, 78% of the larvae were alive and swimming under ambient temperature and ambient pCO2, but only 55-59% were alive in the other treatments. These results demonstrate that the physiological effects of temperature and pCO2 on coral larvae can reliably be detected within days, but effects on survival require > or = 5 days to detect. The detection of time-dependent effects on larval survivorship suggests that the influence of GCC and OA will be stronger for corals having long PLDs.
Keyword(s):
Animalia; Biomass/Abundance/Elemental composition; Cnidaria; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Mortality/Survival; North Pacific; Pelagos; Pocillopora damicornis; Respiration; Single species; Temperate; Temperature; Zooplankton
Further details:
Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4 [webpage]. https://cran.r-project.org/package=seacarb
Coverage:
Latitude: 21.938170 * Longitude: 120.746020
Date/Time Start: 2011-03-02T00:00:00 * Date/Time End: 2011-03-20T00:00:00
Minimum Elevation: -10.0 m * Maximum Elevation: -5.0 m
Event(s):
Nanwan_Bay * Latitude: 21.938170 * Longitude: 120.746020 * Date/Time Start: 2011-03-02T00:00:00 * Date/Time End: 2011-03-20T00:00:00 * Elevation Start: -5.0 m * Elevation End: -10.0 m * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-29.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesCumbo, Vivian R
2DateDateCumbo, Vivian R
3Sample code/labelSample labelCumbo, Vivian Rtank
4ReplicateReplCumbo, Vivian R
5TreatmentTreatCumbo, Vivian R
6Incubation durationInc durdaysCumbo, Vivian R
7Proteins per individualProt/indµg/#Cumbo, Vivian RSpectrophotometric
8Respiration rate, oxygen, per proteinResp O2/protnmol/mg/minCumbo, Vivian RRespirometer (RESP)
9Symbiont cell densitySymbiont#/mgCumbo, Vivian R
10MortalityMortality%Cumbo, Vivian R
11SalinitySalCumbo, Vivian R
12Temperature, waterTemp°CCumbo, Vivian R
13Temperature, water, standard errorT std e±Cumbo, Vivian R
14IrradianceEµmol/m2/sCumbo, Vivian R
15Irradiance, standard errorE std e±Cumbo, Vivian R
16pHpHCumbo, Vivian Rtotal scale
17Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetPaCumbo, Vivian RCalculated
18Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Cumbo, Vivian RCalculated
19Alkalinity, totalATµmol/kgCumbo, Vivian R
20Alkalinity, total, standard errorAT std e±Cumbo, Vivian R
21Aragonite saturation stateOmega ArgCumbo, Vivian RCalculated
22Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
23pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale, in situ
24Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
25Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
26Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
5823 data points

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