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Hu, Marian Y; Sucre, Elliott; Charmantier-Daures, Mireille; Charmantier, Guy; Lucassen, Magnus; Himmerkus, Nina; Melzner, Frank (2015): Localization of ion-regulatory epithelia in embryos and hatchlings of two cephalopods. PANGAEA,, Supplement to: Hu, MY et al. (2010): Localization of ion-regulatory epithelia in embryos and hatchlings of two cephalopods. Cell and Tissue Research, 339(3), 571-583,

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The tissue distribution and ontogeny of Na+/K+-ATPase has been examined as an indicator for ion-regulatory epithelia in whole animal sections of embryos and hatchlings of two cephalopod species: the squid Loligo vulgaris and the cuttlefish Sepia officinalis. This is the first report of the immunohistochemical localization of cephalopod Na+/K+-ATPase with the polyclonal antibody alpha (H-300) raised against the human alpha1-subunit of Na+/K+-ATPase. Na+/K+-ATPase immunoreactivity was observed in several tissues (gills, pancreatic appendages, nerves), exclusively located in baso-lateral membranes lining blood sinuses. Furthermore, large single cells in the gill of adult L. vulgaris specimens closely resembled Na+/K+-ATPase-rich cells described in fish. Immunohistochemical observations indicated that the amount and distribution of Na+/K+-ATPase in late cuttlefish embryos was similar to that found in juvenile and adult stages. The ion-regulatory epithelia (e.g., gills, excretory organs) of the squid embryos and paralarvae exhibited less differentiation than adults. Na+/K+-ATPase activities for whole animals were higher in hatchlings of S. officinalis (157.0 ± 32.4 µmol/g FM/h) than in those of L. vulgaris (31.8 ± 3.3 µmol/g FM/h). S. officinalis gills and pancreatic appendages achieved activities of 94.8 ± 18.5 and 421.8 ± 102.3 µmol ATP/g FM/h, respectively. High concentrations of Na+/K+-ATPase in late cephalopod embryos might be important in coping with the challenging abiotic conditions (low pH, high pCO2) that these organisms encounter inside their eggs. Our results also suggest a higher sensitivity of squid vs. cuttlefish embryos to environmental acid-base disturbances.
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesHu, Marian Y
2TypeTypeHu, Marian Y
3ReplicatesRepl#Hu, Marian Y
4Sodium/Potassium adenosine triphosphatase activityNa/K-ATPase actµmol/g/hHu, Marian Y
5Standard deviationStd dev±Hu, Marian YNa/K-ATPase
6Adenosine triphosphatase activityATPase actµmol/g/hHu, Marian Yother ATPases
7Standard deviationStd dev±Hu, Marian Yother ATPases
8Adenosine triphosphatase activityATPase actµmol/g/hHu, Marian Ytotal ATPase
9Standard deviationStd dev±Hu, Marian Ytotal ATPase
10Change in Sodium/potassium ATPase alpha subunit expressionNaK-ATPase%Hu, Marian Y
40 data points

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