Hu, Marian Y; Sucre, Elliott; Charmantier-Daures, Mireille; Charmantier, Guy; Lucassen, Magnus; Himmerkus, Nina; Melzner, Frank (2015): Localization of ion-regulatory epithelia in embryos and hatchlings of two cephalopods [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.842790, Supplement to: Hu, MY et al. (2010): Localization of ion-regulatory epithelia in embryos and hatchlings of two cephalopods. Cell and Tissue Research, 339(3), 571-583, https://doi.org/10.1007/s00441-009-0921-8
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Abstract:
The tissue distribution and ontogeny of Na+/K+-ATPase has been examined as an indicator for ion-regulatory epithelia in whole animal sections of embryos and hatchlings of two cephalopod species: the squid Loligo vulgaris and the cuttlefish Sepia officinalis. This is the first report of the immunohistochemical localization of cephalopod Na+/K+-ATPase with the polyclonal antibody alpha (H-300) raised against the human alpha1-subunit of Na+/K+-ATPase. Na+/K+-ATPase immunoreactivity was observed in several tissues (gills, pancreatic appendages, nerves), exclusively located in baso-lateral membranes lining blood sinuses. Furthermore, large single cells in the gill of adult L. vulgaris specimens closely resembled Na+/K+-ATPase-rich cells described in fish. Immunohistochemical observations indicated that the amount and distribution of Na+/K+-ATPase in late cuttlefish embryos was similar to that found in juvenile and adult stages. The ion-regulatory epithelia (e.g., gills, excretory organs) of the squid embryos and paralarvae exhibited less differentiation than adults. Na+/K+-ATPase activities for whole animals were higher in hatchlings of S. officinalis (157.0 ± 32.4 µmol/g FM/h) than in those of L. vulgaris (31.8 ± 3.3 µmol/g FM/h). S. officinalis gills and pancreatic appendages achieved activities of 94.8 ± 18.5 and 421.8 ± 102.3 µmol ATP/g FM/h, respectively. High concentrations of Na+/K+-ATPase in late cephalopod embryos might be important in coping with the challenging abiotic conditions (low pH, high pCO2) that these organisms encounter inside their eggs. Our results also suggest a higher sensitivity of squid vs. cuttlefish embryos to environmental acid-base disturbances.
Project(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Species | Species | Hu, Marian Y | |||
| 2 | Type | Type | Hu, Marian Y | |||
| 3 | Replicates | Repl | # | Hu, Marian Y | ||
| 4 | Sodium/Potassium adenosine triphosphatase activity | Na/K-ATPase act | µmol/g/h | Hu, Marian Y | ||
| 5 | Standard deviation | Std dev | ± | Hu, Marian Y | Na/K-ATPase | |
| 6 | Adenosine triphosphatase activity | ATPase act | µmol/g/h | Hu, Marian Y | other ATPases | |
| 7 | Standard deviation | Std dev | ± | Hu, Marian Y | other ATPases | |
| 8 | Adenosine triphosphatase activity | ATPase act | µmol/g/h | Hu, Marian Y | total ATPase | |
| 9 | Standard deviation | Std dev | ± | Hu, Marian Y | total ATPase | |
| 10 | Change in Sodium/potassium ATPase alpha subunit expression | NaK-ATPase | % | Hu, Marian Y |
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
40 data points
Data
| 1 Species | 2 Type | 3 Repl [#] | 4 Na/K-ATPase act [µmol/g/h] | 5 Std dev [±] (Na/K-ATPase) | 6 ATPase act [µmol/g/h] (other ATPases) | 7 Std dev [±] (other ATPases) | 8 ATPase act [µmol/g/h] (total ATPase) | 9 Std dev [±] (total ATPase) | 10 NaK-ATPase [%] |
|---|---|---|---|---|---|---|---|---|---|
| Loligo vulgaris | whole animals | 5 | 31.83 | 3.3 | 341.94 | 59.57 | 373.77 | 60.21 | 8.52 |
| Sepia officinalis | whole animals | 6 | 156.97 | 32.4 | 928.74 | 125.62 | 1106.02 | 132.65 | 14.19 |
| Sepia officinalis | gill | 8 | 94.83 | 18.5 | 404.81 | 66.76 | 499.64 | 75.15 | 18.98 |
| Sepia officinalis | pancreatic appendages | 8 | 421.75 | 102.2 | 661.69 | 66.51 | 1083.44 | 157.37 | 38.92 |