@misc{hu2015loie, author={Marian Y {Hu} and Elliott {Sucre} and Mireille {Charmantier-Daures} and Guy {Charmantier} and Magnus {Lucassen} and Nina {Himmerkus} and Frank {Melzner}}, title={{Localization of ion-regulatory epithelia in embryos and hatchlings of two cephalopods}}, year={2015}, doi={10.1594/PANGAEA.842790}, url={https://doi.org/10.1594/PANGAEA.842790}, note={Supplement to: Hu, MY et al. (2010): Localization of ion-regulatory epithelia in embryos and hatchlings of two cephalopods. Cell and Tissue Research, 339(3), 571-583, https://doi.org/10.1007/s00441-009-0921-8}, abstract={The tissue distribution and ontogeny of Na+/K+-ATPase has been examined as an indicator for ion-regulatory epithelia in whole animal sections of embryos and hatchlings of two cephalopod species: the squid Loligo vulgaris and the cuttlefish Sepia officinalis. This is the first report of the immunohistochemical localization of cephalopod Na+/K+-ATPase with the polyclonal antibody alpha (H-300) raised against the human alpha1-subunit of Na+/K+-ATPase. Na+/K+-ATPase immunoreactivity was observed in several tissues (gills, pancreatic appendages, nerves), exclusively located in baso-lateral membranes lining blood sinuses. Furthermore, large single cells in the gill of adult L. vulgaris specimens closely resembled Na+/K+-ATPase-rich cells described in fish. Immunohistochemical observations indicated that the amount and distribution of Na+/K+-ATPase in late cuttlefish embryos was similar to that found in juvenile and adult stages. The ion-regulatory epithelia (e.g., gills, excretory organs) of the squid embryos and paralarvae exhibited less differentiation than adults. Na+/K+-ATPase activities for whole animals were higher in hatchlings of S. officinalis (157.0 $\pm$ 32.4 $\mathrm{\mu}$mol/g FM/h) than in those of L. vulgaris (31.8 $\pm$ 3.3 $\mathrm{\mu}$mol/g FM/h). S. officinalis gills and pancreatic appendages achieved activities of 94.8 $\pm$ 18.5 and 421.8 $\pm$ 102.3 $\mathrm{\mu}$mol ATP/g FM/h, respectively. High concentrations of Na+/K+-ATPase in late cephalopod embryos might be important in coping with the challenging abiotic conditions (low pH, high pCO2) that these organisms encounter inside their eggs. Our results also suggest a higher sensitivity of squid vs. cuttlefish embryos to environmental acid-base disturbances.}, type={data set}, publisher={PANGAEA} }