Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

Meador, Travis B; Gagen, Emma; Loscar, Michael E; Goldhammer, Tobias; Yoshinaga, Marcos Yukio; Wendt, Jenny; Thomm, Michael; Hinrichs, Kai-Uwe (2014): Polar membrane lipids and elemental composition of Thermococcus kodakarensis cells according to growth stage and phosphorus supply. PANGAEA, https://doi.org/10.1594/PANGAEA.833263, Supplement to: Meador, TB et al. (2014): Thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability. Frontiers in Microbiology, 5, 1-13, https://doi.org/10.3389/fmicb.2014.00010

Always quote citation above when using data! You can download the citation in several formats below.

RIS CitationBibTeX Citation

Abstract:
We observed significant changes in the elemental and intact polar lipid (IPL) composition of the archaeon Thermococcus kodakarensis (KOD1) in response to growth stage and phosphorus supply. Reducing the amount of organic supplements and phosphate in growth media resulted in significant decreases in cell size and cellular quotas of carbon (C), nitrogen (N), and phosphorus (P), which coincided with significant increases in cellular IPL quota and IPLs comprising multiple P atoms and hexose moieties. Relatively more cellular P was stored as IPLs in P-limited cells (2-8%) compared to control cells (<0.8%). We also identified a specific IPL biomarker containing a phosphatidyl-N-acetylhexoseamine headgroup that was relatively enriched during rapid cell division. These observations serve as empirical evidence of IPL adaptations in Archaea that will help to interpret the distribution of these biomarkers in natural systems. The reported cell quotas of C, N, and P represent the first such data for a specific archaeon and suggest that thermophiles are C-rich compared to the cell carbon-to-volume relationship reported for planktonic bacteria.
Comment:
The T. kodakarensis cells were a specific strain grown in a fermenter at the University of Regensburg. Abbreviations as follows: PA-AR = phosphatidic acid archaeol; PG-AR = phosphatidyl glycerol archaeol; 2G-AR = diglycosidic archaeol; PE-AR = phosphatidyl ethanolamine archaeol; P-HexNAc-AR = phosphatidyl N-acetylhexoseamine archaeol; 68+Hex(NH2)-PI-AR = 68 (Da) + hexoseamine + phosphatidyl inositol archaeol; PS-AR = phosphatidyl serine archaeol; PI-AR = phosphatidyl inositol archaeol; Hex(NH2)-PI-AR = hexoseamine + phosphatidyl inositol archaeol; Hex-PI-AR = hexose + phosphatidyl inositol archaeol; PI-Hex(NH2)-PI-AR = hexoseamine + diphosphatidyl inositol archaeol; PG-GDGT = phosphatidyl glycerol glycerol dialkyl glycerol tetraether; 2G-GDGT = diglycosidic glycerol dialkyl glycerol tetraether; 2G-Me-GDGT = diglycosidic methylated glycerol dialkyl glycerol tetraether; 2G-2Me-GDGT = diglycosidic dimethylated glycerol dialkyl glycerol tetraether; PI-GDGT = phosphatidyl inositol glycerol dialkyl glycerol tetraether; Hex(NH2)-PI-GDGT = hexoseamine + phosphatidyl inositol glycerol dialkyl glycerol tetraether; Hex-PI-GDGT-PI = hexose + diphosphatidyl inositol glycerol dialkyl glycerol tetraether; Hex(NH2)-PI-GDGT-PI = hexoseamine + diphosphatidyl inositol glycerol dialkyl glycerol tetraether; PI-GDGT-PI = diphosphatidyl inositol glycerol dialkyl glycerol tetraether
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TreatmentTreatMeador, Travis B
2Growth phaseGrowth phaseMeador, Travis B
3ReplicateReplicateMeador, Travis B
4Incubation durationInc durhMeador, Travis B
5Thermococcus kodakarensisT. kodakarensis#Meador, Travis B
6Dry massDry mgMeador, Travis B
7Carbon, organic, total, content per cellTOC/cellfg/#Meador, Travis B
8Nitrogen, total, content per cellTN/cellfg/#Meador, Travis B
9Phosphorus, total, content per cellTP/cellfg/#Meador, Travis B
10Archaeol content per cellAR/cellfg/#Meador, Travis BPA-AR
11Archaeol content per cellAR/cellfg/#Meador, Travis BPG-AR
12Archaeol content per cellAR/cellfg/#Meador, Travis B2G-AR
13Archaeol content per cellAR/cellfg/#Meador, Travis BPE-AR
14Archaeol content per cellAR/cellfg/#Meador, Travis BP-HexNAc-AR
15Archaeol content per cellAR/cellfg/#Meador, Travis B68+Hex(NH2)-PI-AR
16Archaeol content per cellAR/cellfg/#Meador, Travis BPS-AR
17Archaeol content per cellAR/cellfg/#Meador, Travis BPI-AR
18Archaeol content per cellAR/cellfg/#Meador, Travis BHex(NH2)-PI-AR
19Archaeol content per cellAR/cellfg/#Meador, Travis BHex-PI-AR
20Archaeol content per cellAR/cellfg/#Meador, Travis BPI-Hex(NH2)-PI-AR
21Glycerol dialkyl glycerol tetraether, content per cellGDGT/cellfg/#Meador, Travis BPG-GDGT
22Glycerol dialkyl glycerol tetraether, content per cellGDGT/cellfg/#Meador, Travis B2G-GDGT
23Glycerol dialkyl glycerol tetraether, content per cellGDGT/cellfg/#Meador, Travis B2G-Me-GDGT
24Glycerol dialkyl glycerol tetraether, content per cellGDGT/cellfg/#Meador, Travis B2G-2Me-GDGT
25Glycerol dialkyl glycerol tetraether, content per cellGDGT/cellfg/#Meador, Travis BPI-GDGT
26Glycerol dialkyl glycerol tetraether, content per cellGDGT/cellfg/#Meador, Travis BHex(NH2)-PI-GDGT
27Glycerol dialkyl glycerol tetraether, content per cellGDGT/cellfg/#Meador, Travis BHex-PI-GDGT-PI
28Glycerol dialkyl glycerol tetraether, content per cellGDGT/cellfg/#Meador, Travis BHex(NH2)-PI-GDGT-PI
29Glycerol dialkyl glycerol tetraether, content per cellGDGT/cellfg/#Meador, Travis BPI-GDGT-PI
Size:
458 data points

Download Data

Download dataset as tab-delimited text — use the following character encoding:

View dataset as HTML