/* DATA DESCRIPTION: Citation: Meador, Travis B; Gagen, Emma; Loscar, Michael E; Goldhammer, Tobias; Yoshinaga, Marcos Yukio; Wendt, Jenny; Thomm, Michael; Hinrichs, Kai-Uwe (2014): Polar membrane lipids and elemental composition of Thermococcus kodakarensis cells according to growth stage and phosphorus supply. PANGAEA, https://doi.org/10.1594/PANGAEA.833263, Supplement to: Meador, TB et al. (2014): Thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability. Frontiers in Microbiology, 5, 1-13, https://doi.org/10.3389/fmicb.2014.00010 Abstract: We observed significant changes in the elemental and intact polar lipid (IPL) composition of the archaeon Thermococcus kodakarensis (KOD1) in response to growth stage and phosphorus supply. Reducing the amount of organic supplements and phosphate in growth media resulted in significant decreases in cell size and cellular quotas of carbon (C), nitrogen (N), and phosphorus (P), which coincided with significant increases in cellular IPL quota and IPLs comprising multiple P atoms and hexose moieties. Relatively more cellular P was stored as IPLs in P-limited cells (2-8%) compared to control cells (<0.8%). We also identified a specific IPL biomarker containing a phosphatidyl-N-acetylhexoseamine headgroup that was relatively enriched during rapid cell division. These observations serve as empirical evidence of IPL adaptations in Archaea that will help to interpret the distribution of these biomarkers in natural systems. The reported cell quotas of C, N, and P represent the first such data for a specific archaeon and suggest that thermophiles are C-rich compared to the cell carbon-to-volume relationship reported for planktonic bacteria. Comment: The T. kodakarensis cells were a specific strain grown in a fermenter at the University of Regensburg. Abbreviations as follows: PA-AR = phosphatidic acid archaeol; PG-AR = phosphatidyl glycerol archaeol; 2G-AR = diglycosidic archaeol; PE-AR = phosphatidyl ethanolamine archaeol; P-HexNAc-AR = phosphatidyl N-acetylhexoseamine archaeol; 68+Hex(NH2)-PI-AR = 68 (Da) + hexoseamine + phosphatidyl inositol archaeol; PS-AR = phosphatidyl serine archaeol; PI-AR = phosphatidyl inositol archaeol; Hex(NH2)-PI-AR = hexoseamine + phosphatidyl inositol archaeol; Hex-PI-AR = hexose + phosphatidyl inositol archaeol; PI-Hex(NH2)-PI-AR = hexoseamine + diphosphatidyl inositol archaeol; PG-GDGT = phosphatidyl glycerol glycerol dialkyl glycerol tetraether; 2G-GDGT = diglycosidic glycerol dialkyl glycerol tetraether; 2G-Me-GDGT = diglycosidic methylated glycerol dialkyl glycerol tetraether; 2G-2Me-GDGT = diglycosidic dimethylated glycerol dialkyl glycerol tetraether; PI-GDGT = phosphatidyl inositol glycerol dialkyl glycerol tetraether; Hex(NH2)-PI-GDGT = hexoseamine + phosphatidyl inositol glycerol dialkyl glycerol tetraether; Hex-PI-GDGT-PI = hexose + diphosphatidyl inositol glycerol dialkyl glycerol tetraether; Hex(NH2)-PI-GDGT-PI = hexoseamine + diphosphatidyl inositol glycerol dialkyl glycerol tetraether; PI-GDGT-PI = diphosphatidyl inositol glycerol dialkyl glycerol tetraether Parameter(s): Treatment (Treat) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) Growth phase (Growth phase) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) Replicate (Repl) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) Incubation duration [h] (Inc dur) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) Thermococcus kodakarensis [#] (T. kodakarensis) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) Dry mass [g] (Dry m) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) Carbon, organic, total, per cell [fg/#] (TOC/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) Nitrogen, total, per cell [fg/#] (TN/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) Phosphorus, total, per cell [fg/#] (TP/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) Archaeol, per cell [fg/#] (AR/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: PA-AR Archaeol, per cell [fg/#] (AR/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: PG-AR Archaeol, per cell [fg/#] (AR/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: 2G-AR Archaeol, per cell [fg/#] (AR/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: PE-AR Archaeol, per cell [fg/#] (AR/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: P-HexNAc-AR Archaeol, per cell [fg/#] (AR/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: 68+Hex(NH2)-PI-AR Archaeol, per cell [fg/#] (AR/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: PS-AR Archaeol, per cell [fg/#] (AR/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: PI-AR Archaeol, per cell [fg/#] (AR/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: Hex(NH2)-PI-AR Archaeol, per cell [fg/#] (AR/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: Hex-PI-AR Archaeol, per cell [fg/#] (AR/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: PI-Hex(NH2)-PI-AR Glycerol dialkyl glycerol tetraether, per cell [fg/#] (GDGT/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: PG-GDGT Glycerol dialkyl glycerol tetraether, per cell [fg/#] (GDGT/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: 2G-GDGT Glycerol dialkyl glycerol tetraether, per cell [fg/#] (GDGT/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: 2G-Me-GDGT Glycerol dialkyl glycerol tetraether, per cell [fg/#] (GDGT/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: 2G-2Me-GDGT Glycerol dialkyl glycerol tetraether, per cell [fg/#] (GDGT/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: PI-GDGT Glycerol dialkyl glycerol tetraether, per cell [fg/#] (GDGT/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: Hex(NH2)-PI-GDGT Glycerol dialkyl glycerol tetraether, per cell [fg/#] (GDGT/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: Hex-PI-GDGT-PI Glycerol dialkyl glycerol tetraether, per cell [fg/#] (GDGT/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: Hex(NH2)-PI-GDGT-PI Glycerol dialkyl glycerol tetraether, per cell [fg/#] (GDGT/cell) * PI: Meador, Travis B (https://orcid.org/0000-0001-8255-5883, travis.meador@bc.cas.cz) * COMMENT: PI-GDGT-PI License: Creative Commons Attribution 3.0 Unported (CC-BY-3.0) (URI: https://creativecommons.org/licenses/by/3.0/) Size: 458 data points */ Treat Growth phase Repl Inc dur [h] T. kodakarensis [#] Dry m [g] TOC/cell [fg/#] TN/cell [fg/#] TP/cell [fg/#] AR/cell [fg/#] (PA-AR) AR/cell [fg/#] (PG-AR) AR/cell [fg/#] (2G-AR) AR/cell [fg/#] (PE-AR) AR/cell [fg/#] (P-HexNAc-AR) AR/cell [fg/#] (68+Hex(NH2)-PI-AR) AR/cell [fg/#] (PS-AR) AR/cell [fg/#] (PI-AR) AR/cell [fg/#] (Hex(NH2)-PI-AR) AR/cell [fg/#] (Hex-PI-AR) AR/cell [fg/#] (PI-Hex(NH2)-PI-AR) GDGT/cell [fg/#] (PG-GDGT) GDGT/cell [fg/#] (2G-GDGT) GDGT/cell [fg/#] (2G-Me-GDGT) GDGT/cell [fg/#] (2G-2Me-GDGT) GDGT/cell [fg/#] (PI-GDGT) GDGT/cell [fg/#] (Hex(NH2)-PI-GDGT) GDGT/cell [fg/#] (Hex-PI-GDGT-PI) GDGT/cell [fg/#] (Hex(NH2)-PI-GDGT-PI) GDGT/cell [fg/#] (PI-GDGT-PI) control expo 1 6 1.01E11 0.0741 204 56.0 0.0071 0.0216 0.0063 0.0036 0.1064 0.0038 0.0000 0.4602 0.0343 0.0049 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0055 0.0164 control expo 2 9 1.41E11 0.0832 132 35.0 2.7 0.0035 0.0000 0.0261 0.0028 0.1017 0.0028 0.0019 0.3471 0.0325 0.0034 0.0000 0.0000 0.0131 0.0364 0.0072 0.0000 0.0000 0.0000 0.0001 0.0020 control expo 3 7 9.58E10 0.0657 133 36.0 3.2 0.0000 0.0082 0.0000 0.0000 0.1103 0.0000 0.0000 0.3720 0.0172 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 control stat 2 24 1.30E12 0.8012 224 65.0 6.6 0.0056 0.0000 0.0000 0.0019 0.0398 0.0035 0.0000 0.5910 0.0754 0.0039 0.0000 0.0057 0.0000 0.0051 0.0000 0.0000 0.0000 0.0000 0.0000 0.0048 control stat 3 24 1.62E12 0.7030 180 46.0 4.1 0.0000 0.0103 0.0000 0.0000 0.0027 0.0029 0.0076 0.2647 0.0252 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0197 0.0150 0.0000 0.0000 0.0000 reduced Org expo 1 6 9.19E10 0.3919 82 17.0 0.0000 0.0000 0.0000 0.0000 0.2011 0.0000 0.0000 0.4960 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 reduced Org expo 3 10 1.48E11 0.1879 63 15.0 0.0000 0.0000 0.0000 0.0000 0.1225 0.0079 0.0000 0.6212 0.0679 0.0107 0.0000 0.0000 0.0000 0.0000 0.0000 0.0241 0.0000 0.0000 0.0000 0.0000 reduced Org stat 1 19 5.22E10 0.2393 82 17.0 0.0000 0.0000 0.0015 0.0000 0.0198 0.0000 0.0000 0.7469 0.0456 0.0129 0.0000 0.0000 0.0000 0.0000 0.0000 0.0187 0.0000 0.0000 0.0013 0.0061 reduced Org stat 2 22 9.68E11 0.6138 91 12.0 0.0000 0.0000 0.0000 0.0000 0.0400 0.0000 0.0000 1.1634 0.0851 0.0498 0.0000 0.0000 0.0000 0.0000 0.0000 0.0787 0.0000 0.0000 0.0000 0.0000 reduced Org stat 3 23 1.60E11 0.2546 55 10.0 0.0000 0.0000 0.0000 0.0000 0.0500 0.0110 0.0000 0.8348 0.1894 0.0782 0.0000 0.0000 0.0000 0.0000 0.0000 0.0240 0.0000 0.0000 0.0000 0.0000 reduced Org&P expo 1 7 1.07E11 0.0470 60 13.0 1.2 0.0000 0.0000 0.0000 0.0000 0.0530 0.0077 0.0000 0.6598 0.0614 0.0113 0.0014 0.0000 0.0000 0.0000 0.0000 0.0412 0.0000 0.0000 0.0070 0.0200 reduced Org&P expo 2 6 9.75E10 0.0571 93 20.0 2.0 0.0000 0.0000 0.0000 0.0000 0.0377 0.0168 0.0000 1.1553 0.1764 0.0313 0.0068 0.0000 0.0000 0.0000 0.0000 0.0417 0.0246 0.0000 0.0169 0.0871 reduced Org&P expo 3 5 1.11E11 0.0455 108 26.0 3.2 0.0000 0.0020 0.0000 0.0000 0.0842 0.0231 0.0000 1.1010 0.1361 0.0348 0.0055 0.0000 0.0000 0.0000 0.0000 0.0167 0.0178 0.0000 0.0211 0.0969 reduced Org&P stat 1 24 2.72E11 0.0559 36 7.0 0.6 0.0000 0.0000 0.0000 0.0000 0.0119 0.0048 0.0000 0.3522 0.0703 0.0275 0.0018 0.0000 0.0000 0.0000 0.0000 0.0084 0.0000 0.0062 0.0061 0.0113 reduced Org&P stat 2 24 2.13E11 0.0518 40 7.6 0.3 0.0000 0.0000 0.0000 0.0000 0.0111 0.0083 0.0000 0.5301 0.1165 0.0401 0.0032 0.0000 0.0000 0.0000 0.0000 0.0135 0.0115 0.0117 0.0209 0.0380 reduced Org&P stat 3 22 1.90E11 0.0620 67 10.0 1.6 0.0000 0.0007 0.0000 0.0000 0.0393 0.0145 0.0000 0.6129 0.1882 0.0761 0.0049 0.0000 0.0000 0.0000 0.0000 0.0102 0.0501 0.0115 0.0387 0.0680