Citation:

j.marpolbul.2012.07.043

Name: Analysis of Pacific oyster larval proteome and its response to high-CO2
Published: 2012
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Gene expression (incl. proteomics); Growth/Morphology; Laboratory experiment; Mollusca; North Pacific; Pelagos; Single species; Tropical; Zooplankton

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Abstract:

Most calcifying organisms show depressed metabolic, growth and calcification rates as symptoms to high-CO(2) due to ocean acidification (OA) process. Analysis of the global expression pattern of proteins (proteome analysis) represents a powerful tool to examine these physiological symptoms at molecular level, but its applications are inadequate. To address this knowledge gap, 2-DE coupled with mass spectrophotometer was used to compare the global protein expression pattern of oyster larvae exposed to ambient and to high-CO(2). Exposure to OA resulted in marked reduction of global protein expression with a decrease or loss of 71 proteins (18% of the expressed proteins in control), indicating a wide-spread depression of metabolic genes expression in larvae reared under OA. This is, to our knowledge, the first proteome analysis that provides insights into the link between physiological suppression and protein down-regulation under OA in oyster larvae.

Keyword(s):

Animalia; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Gene expression (incl. proteomics); Growth/Morphology; Laboratory experiment; Mollusca; North Pacific; Pelagos; Single species; Tropical; Zooplankton

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-02-20.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Thiyagarajan, Vengatesen
2	Identification	ID		Thiyagarajan, Vengatesen
3	Duration, number of days	Duration	days	Thiyagarajan, Vengatesen		days after fertilization
4	Replicate	Repl		Thiyagarajan, Vengatesen
5	Treatment	Treat		Thiyagarajan, Vengatesen
6	Shell length	Shell l	mm	Thiyagarajan, Vengatesen
7	Protein name	Protein		Thiyagarajan, Vengatesen
8	Spot intensity, relative	Spot inten		Thiyagarajan, Vengatesen
9	Salinity	Sal		Thiyagarajan, Vengatesen
10	Temperature, water	Temp	°C	Thiyagarajan, Vengatesen
11	Alkalinity, total	AT	µmol/kg	Thiyagarajan, Vengatesen
12	pH	pH		Thiyagarajan, Vengatesen		NBS scale
13	Carbon, inorganic, dissolved	DIC	µmol/kg	Thiyagarajan, Vengatesen	Calculated using CO2SYS
14	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Thiyagarajan, Vengatesen	Calculated using CO2SYS
15	Carbon dioxide	CO2	µmol/kg	Thiyagarajan, Vengatesen	Calculated using CO2SYS
16	Bicarbonate ion	[HCO3]-	µmol/kg	Thiyagarajan, Vengatesen	Calculated using CO2SYS
17	Carbonate ion	[CO3]2-	µmol/kg	Thiyagarajan, Vengatesen	Calculated using CO2SYS
18	Calcite saturation state	Omega Cal		Thiyagarajan, Vengatesen	Calculated using CO2SYS
19	Aragonite saturation state	Omega Arg		Thiyagarajan, Vengatesen	Calculated using CO2SYS
20	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
21	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
22	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
23	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
24	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
25	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
26	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
27	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1861 data points

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