Citation:

s00227-013-2176-x

Name: Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis
Published: 2013
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea hongkongensis; Gene expression (incl. proteomics); Growth/Morphology; Laboratory experiment; Mollusca; North Pacific; Pelagos; Single species; Tropical; Zooplankton

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Abstract:

Ocean acidification (OA) is beginning to have noticeable negative impact on calcification rate, shell structure and physiological energy budgeting of several marine organisms; these alter the growth of many economically important shellfish including oysters. Early life stages of oysters may be particularly vulnerable to OA-driven low pH conditions because their shell is made up of the highly soluble form of calcium carbonate (CaCO3) mineral, aragonite. Our long-term CO2 perturbation experiment showed that larval shell growth rate of the oyster species Crassostrea hongkongensis was significantly reduced at pH < 7.9 compared to the control (8.2). To gain new insights into the underlying mechanisms of low-pH-induced delays in larval growth, we have examined the effect of pH on the protein expression pattern, including protein phosphorylation status at the pediveliger larval stage. Using two-dimensional electrophoresis and mass spectrometry, we demonstrated that the larval proteome was significantly altered by the two low pH treatments (7.9 and 7.6) compared to the control pH (8.2). Generally, the number of expressed proteins and their phosphorylation level decreased with low pH. Proteins involved in larval energy metabolism and calcification appeared to be down-regulated in response to low pH, whereas cell motility and production of cytoskeletal proteins were increased. This study on larval growth coupled with proteome change is the first step toward the search for novel Protein Expression Signatures indicative of low pH, which may help in understanding the mechanisms involved in low pH tolerance.

Keyword(s):

Animalia; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea hongkongensis; Gene expression (incl. proteomics); Growth/Morphology; Laboratory experiment; Mollusca; North Pacific; Pelagos; Single species; Tropical; Zooplankton

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-04-03.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Thiyagarajan, Vengatesen
2	Figure	Fig		Thiyagarajan, Vengatesen
3	Treatment	Treat		Thiyagarajan, Vengatesen
4	Replicates	Repl	#	Thiyagarajan, Vengatesen
5	Incubation duration	Inc dur	days	Thiyagarajan, Vengatesen
6	Shell length	Shell l	mm	Thiyagarajan, Vengatesen		larval
7	Growth rate	µ	mm/day	Thiyagarajan, Vengatesen
8	Growth rate, standard deviation	µ std dev	±	Thiyagarajan, Vengatesen
9	Protein name	Protein		Thiyagarajan, Vengatesen
10	Protein spots, total	Protein spots	#	Thiyagarajan, Vengatesen
11	Protein spots, total, standard deviation	Protein spots std dev	±	Thiyagarajan, Vengatesen
12	Spot intensity, relative	Spot inten		Thiyagarajan, Vengatesen		ratiometric comparison of phosphoprotein/total prtein-14-3-3 protein
13	Spot intensity, relative, standard deviation	Spot inten std dev	±	Thiyagarajan, Vengatesen		ratiometric comparison of phosphoprotein/total prtein-14-3-3 protein
14	Temperature, water	Temp	°C	Thiyagarajan, Vengatesen
15	Salinity	Sal		Thiyagarajan, Vengatesen
16	Alkalinity, total	AT	µmol/kg	Thiyagarajan, Vengatesen	Potentiometric titration
17	pH	pH		Thiyagarajan, Vengatesen	Potentiometric	NBS scale
18	Carbon, inorganic, dissolved	DIC	µmol/kg	Thiyagarajan, Vengatesen	Calculated using CO2SYS
19	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Thiyagarajan, Vengatesen	Calculated using CO2SYS
20	Carbon dioxide	CO2	µmol/kg	Thiyagarajan, Vengatesen	Calculated using CO2SYS
21	Bicarbonate ion	[HCO3]-	µmol/kg	Thiyagarajan, Vengatesen	Calculated using CO2SYS
22	Carbonate ion	[CO3]2-	µmol/kg	Thiyagarajan, Vengatesen	Calculated using CO2SYS
23	Calcite saturation state	Omega Cal		Thiyagarajan, Vengatesen	Calculated using CO2SYS
24	Aragonite saturation state	Omega Arg		Thiyagarajan, Vengatesen	Calculated using CO2SYS
25	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
26	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
27	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

6308 data points

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