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Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen (2016): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.867318, Supplement to: Dineshram, R et al. (2016): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology, 22(6), 2054-2068, https://doi.org/10.1111/gcb.13249

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Abstract:
The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs.
Keyword(s):
Animalia; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Gene expression (incl. proteomics); Laboratory experiment; Mollusca; Mortality/Survival; North Pacific; Pelagos; Salinity; Single species; Temperate; Temperature; Zooplankton
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Coverage:
Latitude: 36.066670 * Longitude: 120.366670
Event(s):
Jiaozhou_Bay * Latitude: 36.066670 * Longitude: 120.366670 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-10-24.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeThiyagarajan, Vengatesenstudy
2SpeciesSpeciesThiyagarajan, Vengatesen
3Registration number of speciesReg spec noThiyagarajan, Vengatesen
4Uniform resource locator/link to referenceURL refThiyagarajan, VengatesenWoRMS Aphia ID
5TreatmentTreatThiyagarajan, Vengatesen
6Accession numberAccess NoThiyagarajan, Vengatesen
7Fold changeFold changeThiyagarajan, Vengatesenlog2 fold change
8IdentificationIDThiyagarajan, Vengatesentank
9IndividualsInd#Thiyagarajan, Vengatesenlive
10IndividualsInd#Thiyagarajan, Vengatesentotal
11SurvivalSurvival%Thiyagarajan, Vengatesen
12Number of expressed proteinsExpress prot#Thiyagarajan, Vengatesendown-regulated proteins
13Number of expressed proteinsExpress prot#Thiyagarajan, Vengatesenup-regulated proteins
14Number of expressed proteinsExpress prot#Thiyagarajan, Vengatesentotal
15ProteinsProtein%Thiyagarajan, Vengatesendown-regulated
16ProteinsProtein%Thiyagarajan, Vengatesenup-regulated
17pHpHThiyagarajan, VengatesenNBS scale
18pH, standard deviationpH std dev±Thiyagarajan, VengatesenNBS scale
19Temperature, waterTemp°CThiyagarajan, Vengatesen
20Temperature, water, standard deviationTemp std dev±Thiyagarajan, Vengatesen
21SalinitySalThiyagarajan, Vengatesen
22Salinity, standard deviationSal std dev±Thiyagarajan, Vengatesen
23Alkalinity, totalATµmol/kgThiyagarajan, Vengatesen
24Alkalinity, total, standard deviationAT std dev±Thiyagarajan, Vengatesen
25Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmThiyagarajan, VengatesenCalculated using CO2SYS
26Carbonate ion[CO3]2-µmol/kgThiyagarajan, VengatesenCalculated using CO2SYS
27Calcite saturation stateOmega CalThiyagarajan, VengatesenCalculated using CO2SYS
28Aragonite saturation stateOmega ArgThiyagarajan, VengatesenCalculated using CO2SYS
29Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
31Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
35Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
36Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
37Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
38Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
269779 data points

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