Anderson, Kelli; Elizur, Abigail (2013): qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.810131, Supplement to: Anderson, K; Elizur, A (2012): Hepatic reference gene selection in adult and juvenile female Atlantic salmon at normal and elevated temperatures. BMC Research Notes, 5(21), 1-9, https://doi.org/10.1186/1756-0500-5-21
Always quote citation above when using data! You can download the citation in several formats below.
Abstract:
The use of quantitative real-time polymerase chain reaction (qPCR) has become widespread due to its specificity, sensitivity and apparent ease of use. However, experimental error can be introduced at many stages during sample processing and analysis, and for this reason qPCR data are often normalised to an internal reference gene. The present study used three freely available algorithms (GeNorm, NormFinder and BestKeeper) to assess the stability of hepatically expressed candidate reference genes (Hprt1, Tbp, Ef1a and b-tubulin) in two experiments. In the first, female Atlantic salmon (Salmo salar) broodstock of different ages were reared at either 14 or 22°C for an entire reproductive season, therefore a reference gene that does not respond to thermal challenge or reproductive condition was sought. In the second, estrogen treated juvenile salmon were maintained at the same temperatures for 14 days and a reference gene that does not respond to temperature or estrogen was required. Additionally, we performed independent statistic analysis to validate the outputs obtained from the program based analysis.
Coverage:
Date/Time Start: 2007-01-08T00:00:00 * Date/Time End: 2009-01-02T00:00:00
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
2 datasets
Download Data
Datasets listed in this publication series
- Anderson, K; Elizur, A (2011): qPCR counts of mRNA for hepatic reference gene selection in adult female Atlantic salmon from perturbation experiments under artificial temperature conditions. https://doi.org/10.1594/PANGAEA.772212
- Anderson, K; Elizur, A (2011): qPCR counts of mRNA for hepatic reference gene selection in juvenile female Atlantic salmon from perturbation experiments under artificial temperature conditions. https://doi.org/10.1594/PANGAEA.772213