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Anderson, Kelli; Elizur, Abigail (2011): qPCR counts of mRNA for hepatic reference gene selection in juvenile female Atlantic salmon from perturbation experiments under artificial temperature conditions. University of the Sunshine Coast, PANGAEA, https://doi.org/10.1594/PANGAEA.772213, In supplement to: Anderson, K; Elizur, A (2012): Hepatic reference gene selection in adult and juvenile female Atlantic salmon at normal and elevated temperatures. BMC Research Notes, 5(21), 1-9, https://doi.org/10.1186/1756-0500-5-21

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Abstract:
The dataset contains raw data (quantification cycle) for a study which determined the most suitable hepatic reference genes for normalisation of qPCR data orginating from juvenile Atlantic salmon (14 days) exposed to 14 and 22 degrees C. These results will be useful for anyone wanting to study the effects of climate change/elevated temperature on reproductive physiology of fish (and perhaphs other vertebrates).
Coverage:
Date/Time Start: 2009-01-02T00:00:00 * Date/Time End: 2009-01-02T00:00:00
Event(s):
SALTAS_Experimental_Tank(14degC)_February_2009 * Date/Time: 2009-02-02T00:00:00 * Device: Experimental tank (1 cubic metre) under artificial conditions (Exp_Tank_1m3)
SALTAS_Experimental_Tank(22degC)_February_2009 * Date/Time: 2009-02-02T00:00:00 * Device: Experimental tank (1 cubic metre) under artificial conditions (Exp_Tank_1m3)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Event labelEvent
2DATE/TIMEDate/TimeGeocode
3Treatment: durationT:durationdaysAnderson, KelliDuration of the treatment
4Treatment: hormonesT:hormonesAnderson, KelliHormones used as treatment
5Treatment: temperatureTreat temp°CAnderson, KelliTemperature used as treatment
6Life Cycle: stateLC:stateAnderson, KelliState of the Life cycle
7qPCR counts, meanCq#Anderson, KelliFluorescence-based quantitative real-time PCR (qPCR)of mRNA [vernacular: mRNA abundance] expressing gene Tbp [GenBank: BT059217] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon]
8qPCR counts, standard error of meanCq, SEM±Anderson, KelliFluorescence-based quantitative real-time PCR (qPCR)of mRNA [vernacular: mRNA abundance] expressing gene Tbp [GenBank: BT059217] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon]
9qPCR counts, meanCq#Anderson, KelliFluorescence-based quantitative real-time PCR (qPCR)of mRNA [vernacular: mRNA abundance] expressing gene Hprt1 [GenBank: BT043501] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon]
10qPCR counts, standard error of meanCq, SEM±Anderson, KelliFluorescence-based quantitative real-time PCR (qPCR)of mRNA [vernacular: mRNA abundance] expressing gene Hprt1 [GenBank: BT043501] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon]
11qPCR counts, meanCq#Anderson, KelliFluorescence-based quantitative real-time PCR (qPCR)of mRNA [vernacular: mRNA abundance] expressing gene Efla [GenBank: AF321836] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon]
12qPCR counts, standard error of meanCq, SEM±Anderson, KelliFluorescence-based quantitative real-time PCR (qPCR)of mRNA [vernacular: mRNA abundance] expressing gene Efla [GenBank: AF321836] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon]
Size:
120 data points

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