Anderson, Kelli; Elizur, Abigail (2011): qPCR counts of mRNA for hepatic reference gene selection in adult female Atlantic salmon from perturbation experiments under artificial temperature conditions [dataset]. University of the Sunshine Coast, PANGAEA, https://doi.org/10.1594/PANGAEA.772212, In supplement to: Anderson, K; Elizur, A (2012): Hepatic reference gene selection in adult and juvenile female Atlantic salmon at normal and elevated temperatures. BMC Research Notes, 5(21), 1-9, https://doi.org/10.1186/1756-0500-5-21
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Abstract:
The dataset contains raw data (quantification cycle) for a study which determined the most suitable hepatic reference genes for normalisation of qPCR data orginating from adult (entire reproductive season) Atlantic salmon (14 days) exposed to 14 and 22 degrees C. These results will be useful for anyone wanting to study the effects of climate change/elevated temperature on reproductive physiology of fish (and perhaphs other vertebrates). In addition, a target gene (vitellogenin) has normalised using an inappropriate and an 'ideal' reference gene to demonstrate the consequences of using an unstable reference gene for normalisation.
For the adult experiment, maiden and repeat adult females were held at the Salmon Enterprises of Tasmania (SALTAS) Wayatinah Hatchery (Tasmania, Australia) at ambient temperature and photoperiod in either 200 (maidens) or 50 (repeats) m3 circular tanks at stocking densities of 12-18, and 24-36 kg m-3 for maidens and repeats, respectively, until transfered to the experimental tanks.
Coverage:
Date/Time Start: 2007-01-08T00:00:00 * Date/Time End: 2008-01-04T00:00:00
Event(s):
SALTAS_Experimental_Tank(14degC)_April_2008 * Date/Time: 2008-04-01T00:00:00 * Method/Device: Experimental tank (4 cubic metre) under artificial conditions (Exp_Tank_4m3) * Comment: Fish were transferred to temperature-controlled 4 m3 tanks (14 fish per tank) under simulated ambient photoperiod and 14 °C. Fish were not fed from the time of transfer to the temperature controlled systems.
SALTAS_Experimental_Tank(14degC)_August_2007 * Date/Time: 2007-08-01T00:00:00 * Method/Device: Experimental tank (4 cubic metre) under artificial conditions (Exp_Tank_4m3) * Comment: Fish were transferred to temperature-controlled 4 m3 tanks (14 fish per tank) under simulated ambient photoperiod and 14 °C. Fish were not fed from the time of transfer to the temperature controlled systems.
SALTAS_Experimental_Tank(14degC)_February_2008 * Date/Time: 2008-02-01T00:00:00 * Method/Device: Experimental tank (4 cubic metre) under artificial conditions (Exp_Tank_4m3) * Comment: Fish were transferred to temperature-controlled 4 m3 tanks (14 fish per tank) under simulated ambient photoperiod and 14 °C. Fish were not fed from the time of transfer to the temperature controlled systems.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | ||||
| 2 | DATE/TIME | Date/Time | Geocode | |||
| 3 | Treatment: temperature | T:temp | °C | Anderson, Kelli | Temperature used as treatment | |
| 4 | Life Cycle: state | LC:state | Anderson, Kelli | State of the Life cycle | ||
| 5 | qPCR counts, mean | Cq | # | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene Tbp [GenBank: BT059217] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] |
| 6 | qPCR counts, standard error of mean | Cq SEM | ± | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene Tbp [GenBank: BT059217] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] |
| 7 | qPCR counts, mean | Cq | # | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene Hprt1 [GenBank: BT043501] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] |
| 8 | qPCR counts, standard error of mean | Cq SEM | ± | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene Hprt1 [GenBank: BT043501] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] |
| 9 | qPCR counts, mean | Cq | # | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene Efla [GenBank: AF321836] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] |
| 10 | qPCR counts, standard error of mean | Cq SEM | ± | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene Efla [GenBank: AF321836] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] |
| 11 | qPCR counts, mean | Cq | # | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene ß-tubulin [GenBank: DQ367888] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] |
| 12 | qPCR counts, standard error of mean | Cq SEM | ± | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene ß-tubulin [GenBank: DQ367888] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] |
| 13 | qPCR counts, ratio | Cq/Cq | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene Vtg [GenBank: DQ834857] relative to mRNA expressing Tbp [GenBank: BT059217] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] | |
| 14 | qPCR counts, ratio, standard error of mean | Cq/Cq SEM | ± | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene Vtg [GenBank: DQ834857] relative to mRNA expressing Tbp [GenBank: BT059217] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] |
| 15 | qPCR counts, ratio | Cq/Cq | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene Vtg [GenBank: DQ834857] relative to mRNA expressing ß-tubulin [GenBank: DQ367888] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] | |
| 16 | qPCR counts, ratio, standard error of mean | Cq/Cq SEM | ± | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene Vtg [GenBank: DQ834857] relative to mRNA expressing ß-tubulin [GenBank: DQ367888] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] |
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
252 data points