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Petersen, Jillian M; Zielinski, Frank U; Pape, Thomas; Seifert, Richard; Moraru, Cristina; Amann, Rudolf; Hourdez, Stéphane; Girguis, Peter R; Wankel, Scott D; Barbe, Valerie; Pelletier, Eric; Fink, Dennis; Borowski, Christian; Bach, Wolfgang; Dubilier, Nicole (2013): Hydrogen consumption by gills of Bathymodiolus spp. collected at the Comfortless cove and Lilliput hydrothermal vent fields in May 2006 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.808951, In supplement to: Petersen, JM et al. (2011): Hydrogen is an energy source for hydrothermal vent symbioses. Nature, 476, 176-180, https://doi.org/10.1038/nature10325

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Published: 2013-03-16DOI registered: 2013-05-09

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Abstract:
The discovery of deep-sea hydrothermal vents in 1977 revolutionized our understanding of the energy sources that fuel primary productivity on Earth. Hydrothermal vent ecosystems are dominated by animals that live in symbiosis with chemosynthetic bacteria. So far, only two energy sources have been shown to power chemosynthetic symbioses: reduced sulphur compounds and methane. Using metagenome sequencing, single-gene fluorescence in situ hybridization, immunohistochemistry, shipboard incubations and in situ mass spectrometry, we show here that the symbionts of the hydrothermal vent mussel Bathymodiolus from the Mid-Atlantic Ridge use hydrogen to power primary production. In addition, we show that the symbionts of Bathymodiolus mussels from Pacific vents have hupL, the key gene for hydrogen oxidation. Furthermore, the symbionts of other vent animals such as the tubeworm Riftia pachyptila and the shrimp Rimicaris exoculata also have hupL. We propose that the ability to use hydrogen as an energy source is widespread in hydrothermal vent symbioses, particularly at sites where hydrogen is abundant.
Related to:
Koschinsky, Andrea; Billings, Andrew; Devey, Colin W; Dubilier, Nicole; Duester, Alan; Edge, David; Garbe-Schönberg, Dieter; German, Christopher R; Giere, Olav; Keir, Robin; Lackschewitz, Klas Sven; Mai, Anh Hoang; Marbler, Herwig; Mawick, Jule; Melchert, Bernd; Mertens, Christian; Ochsenhirt, Wolf-Thilo; Peters, Marc; Sander, Sylvia; Schmale, Oliver; Schmidt, Werner; Seifert, Richard; Seiter, Christian; Stöber, Uwe; Suck, Inken; Walter, Maren; Weber, Stefan; Yoerger, Dana; Zarrouk, Marcel; Zielinski, Frank (2006): Mid-Atlantic Expedition 2006, Cruise No. 68, Leg 1, Fluid geochemistry, biology and geological setting of hydrothermal systems at the southern MAR (4°S - 10°S) (MAR-SÜD III), 27 April - 2 June 2006, Bridgetown (Barbados) - Recife (Brazil). METEOR-Berichte, Leitstelle Meteor, Institut für Meereskunde der Universität Hamburg, 06, 189 pp, https://doi.org/10.2312/cr_m68-1
Koschinsky, Andrea; Billings, Andrew; Devey, Colin W; Dubilier, Nicole; Duester, Alan; Edge, David; Garbe-Schönberg, Dieter; German, Christopher R; Giere, Olav; Keir, Robin; Lackschewitz, Klas Sven; Mai, Anh Hoang; Marbler, Herwig; Mawick, Jule; Melchert, Bernd; Mertens, Christian; Peters, Michael; Sander, Sylvia; Schmale, Oliver; Schmidt, Werner; Seifert, Richard; Seiter, Christian; Stöber, Uwe; Suck, Inken; Walter, Maren; Weber, Stefan; Yoerger, Dana; Zarrouk, Marcel; Zielinski, Frank (2006): Discovery of new hydrothermal vent fields on the Southern Mid-Atlantic Ridge (4°S - 10°S) during Cruise M68/1. InterRidge News, 15, 9-15, hdl:10013/epic.41214.d001
Zielinski, Frank (2009): Geobiological coupling of hydrothermal vent fluids with endosymbiotic primary producers of bathymodiolus mussels from hydrothermal vents on the Mid-Atlantic Ridge [dissertation]. University of Bremen, Germany, 159 pp, urn:nbn:de:gbv:46-diss000115375
Funding:
German Research Foundation (DFG), grant/award no. 5471797: From Mantle to Ocean: Energy-, Material- and Life-cycles at Spreading Axes
Coverage:
Median Latitude: -7.176584 * Median Longitude: -12.791274 * South-bound Latitude: -9.553380 * West-bound Longitude: -13.209130 * North-bound Latitude: -4.802330 * East-bound Longitude: -12.371500
Date/Time Start: 2006-05-14T11:06:00 * Date/Time End: 2006-05-27T13:45:00
Minimum Elevation: -2993.0 m * Maximum Elevation: -1475.0 m
Event(s):
M68/1-20-ROV  * Latitude Start: -4.802830 * Longitude Start: -12.372500 * Latitude End: -4.802830 * Longitude End: -12.371500 * Date/Time: 2006-05-14T11:06:00 * Elevation: -2993.0 m * Campaign: M68/1 (MARSUED3) * Basis: Meteor (1986) * Method/Device: Remote operated vehicle (ROV) * Comment: Twin Peaks
M68/1-24-ROV  * Latitude Start: -4.802330 * Longitude Start: -12.382000 * Latitude End: -4.803000 * Longitude End: -12.371500 * Date/Time Start: 2006-05-15T15:06:00 * Date/Time End: 2006-05-16T01:32:00 * Elevation Start: -2993.0 m * Elevation End: -2991.0 m * Campaign: M68/1 (MARSUED3) * Basis: Meteor (1986) * Method/Device: Remote operated vehicle (ROV) * Comment: Turtle Pits area
M68/1-39-ROV  * Latitude Start: -9.553380 * Longitude Start: -13.208560 * Latitude End: -9.548630 * Longitude End: -13.209130 * Date/Time Start: 2006-05-18T19:24:00 * Date/Time End: 2006-05-19T00:01:00 * Elevation Start: -1475.0 m * Elevation End: -1494.0 m * Campaign: M68/1 (MARSUED3) * Basis: Meteor (1986) * Method/Device: Remote operated vehicle (ROV) * Comment: S of Liliput, 2nd mussel bed
Comment:
Hydrogen consumption incubations
Sampling site:
For on-board physiological experiments specimens belonging to undescribed Bathymodiolus species (B. spp.) were collected at the Comfortless Cove and Lilliput hydrothermal vent fields on the southern Mid-Atlantic Ridge. Mussels were sampled using nets (40 cm length, 20 cm diameter opening, mesh size 335 µm, Hydrobios, Kiel, Germany) handled by the manipulator arm of the remotely operated vehicles (ROVs) Quest 4000 m (MARUM, Bremen, Germany).
Mussel dissection for shipboard incubations:
Mussels were opened with a scalpel by cutting through the posterior and anterior adductor muscles. Viability was tested by prodding the foot with a dissection needle and only mussels whose foot contracted were used. The foot and both gills were separated from the remaining tissue using dissection scissors. Tissue pieces of 6 mm in diameter were cut out of the gill and the foot tissues using a steel hole-puncher. One tissue piece from each individual was frozen in liquid nitrogen for weight determination in the home laboratory. For negative controls, foot tissues were used which do not contain endosymbiotic bacteria.
Experimental setup:
For hydrogen partial pressures up to 1,000 ppm (in helium) glass serum vials (58 ml) were fully filled with chilled (4°C) sterile-filtered (0.22 µm) bottom seawater retrieved from 3,000 m water depth. One piece of gill tissue was placed in the vial, the vial closed with a gas-tight butyl rubber stopper, and crimped with aluminium seals. Control vials contained foot tissue or no tissue at all. 20 ml of hydrogen gas (100 ppm, 250 ppm or 1,000 ppm in helium, Linde) were injected through the rubber stopper using a gas-tight syringe (SGE Analytical Science and Hamilton) with a second syringe allowing pressure compensation through the outflow of the same volume of seawater. For hydrogen partial pressures above 1,000 ppm (in air) glass serum vials were processed as described above with the exception that the vials were filled with only 38 ml of seawater. Pure hydrogen gas (100%; Air Liquide) was then injected in the air headspace through the rubber stopper to the desired final partial pressure (1,000-3,000 ppm) using a gas-tight syringe. All vials were placed upside down to minimize possible gas exchange via the rubber stopper and incubated at 4°C on a slowly rotating table. At given time points a subsample was taken with a gas-tight syringe from the headspace with the pressure decrease compensated through the inflow of oxygen-saturated, chilled sterile-filtered seawater from a second syringe.
Analysis of the headspace hydrogen content:
The hydrogen concentration in the headspace was determined using a gas chromatograph (Thermo Trace GC ultra) equipped with a packed stainless steel column (Molecular Sieve 5A, carrier gas: He) and a pulse discharge detector (PDD). Recording and calculation of results was performed using a PC operated integration system (Thermo Chrom Card A/D). Analytical procedures were calibrated daily with commercial gas standards (Linde).
Incubation conditions and rate calculations:
The concentrations of dissolved hydrogen and oxygen were calculated from Crozier and Yamamoto (1974) [doi:10.1021/je60062a007] and Weiss (1970) [doi:10.1016/0011-7471(70)90037-9] under the assumption of Henry's law i.e. that the concentration of a dissolved gas in a solution is directly proportional to the partial pressure of that gas above the solution. The molar volume of an ideal gas (22.414 l mol-1) was used to convert between the partial pressure of a gas (ppm) and the amount of the gas (in moles) in the headspace. The rates of hydrogen removal from the headspace [in nmol h-1 (piece tissue)-1] were calculated for the first 60 minutes after addition of the electron source performing non-linear regression through the data points obtained during the complete incubation period of up to 60 hours. Exponential and hyperbolic regression described by the equations f=a*exp(b/(x+c)) and f=y0+(a*b)/(b+x) gave well-fitting regression curves (R2 = 0.920 and 0.921, respectively; standard error of estimate 6.434 and 6.400, respectively). The effect of methodological hydrogen removal from the headspace through subsampling was considered. The rates at which hydrogen disappeared from incubation vials containing only seawater but no tissues (chemical oxidation, hydrogen loss by diffusion) were subtracted from the tissue rates. Resulting rates were then normalized to gram wet weight (in nmol h-1 [g wet weight]-1).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
Event labelEvent
Sample IDSample IDZielinski, Frank
Time in minutesTime minminZielinski, Frank
HydrogenH2ppmvZielinski, Frankheadspace
Hydrogen concentrationH2nmolZielinski, Frankdissolved
Wet massWet mmgZielinski, Frank
Tissue piece, number ofTissue p#Zielinski, Frank
Hydrogen consumption rateH2 cons ratenmol/hZielinski, Frankper gill tissue piece
Hydrogen consumption rate per weightH2 cons ratenmol/h/gZielinski, Franknormalized to wet weight
10 CommentCommentZielinski, Frank
Size:
1582 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Event

Sample ID

Time min [min]

H2 [ppmv]

H2 [nmol]

Wet m [mg]

Tissue p [#]

H2 cons rate [nmol/h]

H2 cons rate [nmol/h/g]
10 
Comment
M68/1-20-ROV 20ROV/1-1 gill0H2-Spike
M68/1-20-ROV20ROV/1-1 gill49573
M68/1-20-ROV20ROV/1-1 gill1049171
M68/1-20-ROV20ROV/1-1 gill2448768
M68/1-20-ROV20ROV/1-1 gill4248465
M68/1-20-ROV20ROV/1-1 gill6648062
M68/1-20-ROV20ROV/1-1 gill9647457
M68/1-20-ROV20ROV/1-1 gill16987255
M68/1-20-ROV20ROV/1-1 gill23036651
M68/1-20-ROV20ROV/1-1 gill38834938
M68/1-20-ROV20ROV/1-1 gill53582822
M68/1-20-ROV20ROV/1-1 gill38.410.6212.23
M68/1-20-ROV20ROV/1-2 gill0H2-Spike
M68/1-20-ROV20ROV/1-2 gill29372
M68/1-20-ROV20ROV/1-2 gill2428465
M68/1-20-ROV20ROV/1-2 gill4227659
M68/1-20-ROV20ROV/1-2 gill6627256
M68/1-20-ROV20ROV/1-2 gill9727054
M68/1-20-ROV20ROV/1-2 gill16965744
M68/1-20-ROV20ROV/1-2 gill23014535
M68/1-20-ROV20ROV/1-2 gill38813124
M68/1-20-ROV20ROV/1-2 gill53562015
M68/1-20-ROV20ROV/1-2 gill36.011.8547.07
M68/1-20-ROV20ROV/1-1 foot0H2-Spike
M68/1-20-ROV20ROV/1-1 foot29775
M68/1-20-ROV20ROV/1-1 foot1029674
M68/1-20-ROV20ROV/1-1 foot2429372
M68/1-20-ROV20ROV/1-1 foot4229170
M68/1-20-ROV20ROV/1-1 foot6629170
M68/1-20-ROV20ROV/1-1 foot9729069
M68/1-20-ROV20ROV/1-1 foot16968868
M68/1-20-ROV20ROV/1-1 foot23018667
M68/1-20-ROV20ROV/1-1 foot38818566
M68/1-20-ROV20ROV/1-1 foot53568062
M68/1-20-ROV20ROV/1-1 foot10.10not applicable
M68/1-20-ROV(20ROV/1) seawater control0H2-Spike
M68/1-20-ROV(20ROV/1) seawater control29674
M68/1-20-ROV(20ROV/1) seawater control1029171
M68/1-20-ROV(20ROV/1) seawater control2429271
M68/1-20-ROV(20ROV/1) seawater control4229069
M68/1-20-ROV(20ROV/1) seawater control6629170
M68/1-20-ROV(20ROV/1) seawater control9729070
M68/1-20-ROV(20ROV/1) seawater control16969070
M68/1-20-ROV(20ROV/1) seawater control23018768
M68/1-20-ROV(20ROV/1) seawater control38818566
M68/1-20-ROV(20ROV/1) seawater control53568263
M68/1-20-ROV(20ROV/1) seawater control0.07not applicable
M68/1-20-ROV20ROV/1-1 gill0H2-Spike
M68/1-20-ROV20ROV/1-1 gill2938727
M68/1-20-ROV20ROV/1-1 gill102902699
M68/1-20-ROV20ROV/1-1 gill242891690
M68/1-20-ROV20ROV/1-1 gill422843654
M68/1-20-ROV20ROV/1-1 gill662837649
M68/1-20-ROV20ROV/1-1 gill972810627
M68/1-20-ROV20ROV/1-1 gill1696747579
M68/1-20-ROV20ROV/1-1 gill2301670519
M68/1-20-ROV20ROV/1-1 gill3881429332
M68/1-20-ROV20ROV/1-1 gill5356325251
M68/1-20-ROV20ROV/1-1 gill38.415.274.29
M68/1-20-ROV20ROV/1-2 gill0H2-Spike
M68/1-20-ROV20ROV/1-2 gill2949735
M68/1-20-ROV20ROV/1-2 gill102905702
M68/1-20-ROV20ROV/1-2 gill242892691
M68/1-20-ROV20ROV/1-2 gill422824639
M68/1-20-ROV20ROV/1-2 gill662787610
M68/1-20-ROV20ROV/1-2 gill972735570
M68/1-20-ROV20ROV/1-2 gill1696652505
M68/1-20-ROV20ROV/1-2 gill2301560434
M68/1-20-ROV20ROV/1-2 gill3881362281
M68/1-20-ROV20ROV/1-2 gill5356299232
M68/1-20-ROV20ROV/1-2 gill36.0111.4210.44
M68/1-20-ROV20ROV/1-1 foot0H2-Spike
M68/1-20-ROV20ROV/1-1 foot2966748
M68/1-20-ROV20ROV/1-1 foot102910705
M68/1-20-ROV20ROV/1-1 foot242906702
M68/1-20-ROV20ROV/1-1 foot422905702
M68/1-20-ROV20ROV/1-1 foot662905701
M68/1-20-ROV20ROV/1-1 foot972904700
M68/1-20-ROV20ROV/1-1 foot1696898696
M68/1-20-ROV20ROV/1-1 foot2301880682
M68/1-20-ROV20ROV/1-1 foot3881847657
M68/1-20-ROV20ROV/1-1 foot5356800620
M68/1-20-ROV20ROV/1-1 foot10.63not applicable
M68/1-20-ROV(20ROV/1) seawater control0H2-Spike
M68/1-20-ROV(20ROV/1) seawater control2987765
M68/1-20-ROV(20ROV/1) seawater control102936725
M68/1-20-ROV(20ROV/1) seawater control242938727
M68/1-20-ROV(20ROV/1) seawater control422932722
M68/1-20-ROV(20ROV/1) seawater control662927718
M68/1-20-ROV(20ROV/1) seawater control972910705
M68/1-20-ROV(20ROV/1) seawater control1696830643
M68/1-20-ROV(20ROV/1) seawater control2301834646
M68/1-20-ROV(20ROV/1) seawater control3881820635
M68/1-20-ROV(20ROV/1) seawater control5356795616
M68/1-20-ROV(20ROV/1) seawater control1.09not applicable
M68/1-24-ROV 24ROV/5-1 gill0H2-Spike
M68/1-24-ROV24ROV/5-1 gill2926718
M68/1-24-ROV24ROV/5-1 gill92920713
M68/1-24-ROV24ROV/5-1 gill282881683
M68/1-24-ROV24ROV/5-1 gill477849658
M68/1-24-ROV24ROV/5-1 gill707820636
M68/1-24-ROV24ROV/5-1 gill1052782606
M68/1-24-ROV24ROV/5-1 gill2242674522
M68/1-24-ROV24ROV/5-1 gill3762579448
M68/1-24-ROV24ROV/5-1 gill5407445345
M68/1-24-ROV24ROV/5-1 gill24.016.97249.67
M68/1-24-ROV24ROV/5-2a gill0H2-Spike
M68/1-24-ROV24ROV/5-2a gill2972753
M68/1-24-ROV24ROV/5-2a gill92938727
M68/1-24-ROV24ROV/5-2a gill282888689
M68/1-24-ROV24ROV/5-2a gill497867672
M68/1-24-ROV24ROV/5-2a gill727793614
M68/1-24-ROV24ROV/5-2a gill1072806625
M68/1-24-ROV24ROV/5-2a gill2262725562
M68/1-24-ROV24ROV/5-2a gill3782569441
M68/1-24-ROV24ROV/5-2a gill5427424328
M68/1-24-ROV24ROV/5-2a gill30.316.94196.65
M68/1-24-ROV24ROV/5-2b gill0H2-Spike
M68/1-24-ROV24ROV/5-2b gill2822637
M68/1-24-ROV24ROV/5-2b gill92821636
M68/1-24-ROV24ROV/5-2b gill102793614
M68/1-24-ROV24ROV/5-2b gill272772598
M68/1-24-ROV24ROV/5-2b gill477758587
M68/1-24-ROV24ROV/5-2b gill707749580
M68/1-24-ROV24ROV/5-2b gill1062707548
M68/1-24-ROV24ROV/5-2b gill2292650504
M68/1-24-ROV24ROV/5-2b gill3762617478
M68/1-24-ROV24ROV/5-2b gill5407567439
M68/1-24-ROV24ROV/5-2b gill30.316.74190.19
M68/1-24-ROV24ROV/5-1a foot0H2-Spike
M68/1-24-ROV24ROV/5-1a foot2978758
M68/1-24-ROV24ROV/5-1a foot92950736
M68/1-24-ROV24ROV/5-1a foot282931721
M68/1-24-ROV24ROV/5-1a foot487903700
M68/1-24-ROV24ROV/5-1a foot717889689
M68/1-24-ROV24ROV/5-1a foot1062896694
M68/1-24-ROV24ROV/5-1a foot2252838650
M68/1-24-ROV24ROV/5-1a foot3772810627
M68/1-24-ROV24ROV/5-1a foot5417770597
M68/1-24-ROV24ROV/5-1a foot11.19not applicable
M68/1-24-ROV24ROV/5-1b foot0H2-Spike
M68/1-24-ROV24ROV/5-1b foot2904701
M68/1-24-ROV24ROV/5-1b foot102797617
M68/1-24-ROV24ROV/5-1b foot272805624
M68/1-24-ROV24ROV/5-1b foot477797617
M68/1-24-ROV24ROV/5-1b foot707807626
M68/1-24-ROV24ROV/5-1b foot1062783607
M68/1-24-ROV24ROV/5-1b foot2292762591
M68/1-24-ROV24ROV/5-1b foot3762756586
M68/1-24-ROV24ROV/5-1b foot5407649503
M68/1-24-ROV24ROV/5-1b foot11.01not applicable
M68/1-24-ROV(24ROV/5) seawater control A0H2-Spike
M68/1-24-ROV(24ROV/5) seawater control A2983762
M68/1-24-ROV(24ROV/5) seawater control A92936725
M68/1-24-ROV(24ROV/5) seawater control A282919712
M68/1-24-ROV(24ROV/5) seawater control A487923716
M68/1-24-ROV(24ROV/5) seawater control A717916710
M68/1-24-ROV(24ROV/5) seawater control A1062904700
M68/1-24-ROV(24ROV/5) seawater control A2252877680
M68/1-24-ROV(24ROV/5) seawater control A3772848657
M68/1-24-ROV(24ROV/5) seawater control A5417798618
M68/1-24-ROV(24ROV/5) seawater control A0.88not applicable
M68/1-24-ROV(24ROV/5) seawater control B0H2-Spike
M68/1-24-ROV(24ROV/5) seawater control B2893692
M68/1-24-ROV(24ROV/5) seawater control B102880682
M68/1-24-ROV(24ROV/5) seawater control B272867672
M68/1-24-ROV(24ROV/5) seawater control B477878680
M68/1-24-ROV(24ROV/5) seawater control B707873677
M68/1-24-ROV(24ROV/5) seawater control B1062857664
M68/1-24-ROV(24ROV/5) seawater control B2292826640
M68/1-24-ROV(24ROV/5) seawater control B3762806624
M68/1-24-ROV(24ROV/5) seawater control B5407708549
M68/1-24-ROV(24ROV/5) seawater control B1.07not applicable
M68/1-39-ROV 39ROV/6-1a gill0H2-Spike
M68/1-39-ROV39ROV/6-1a gill29473
M68/1-39-ROV39ROV/6-1a gill928868
M68/1-39-ROV39ROV/6-1a gill1828969
M68/1-39-ROV39ROV/6-1a gill2948365
M68/1-39-ROV39ROV/6-1a gill6348062
M68/1-39-ROV39ROV/6-1a gill9797457
M68/1-39-ROV39ROV/6-1a gill17346953
M68/1-39-ROV39ROV/6-1a gill21.511.6569.75
M68/1-39-ROV39ROV/6-1b gill1812H2-Spike
M68/1-39-ROV39ROV/6-1b gill1814136105
M68/1-39-ROV39ROV/6-1b gill189912799
M68/1-39-ROV39ROV/6-1b gill232411388
M68/1-39-ROV39ROV/6-1b gill264910078
M68/1-39-ROV39ROV/6-1b gill32998263
M68/1-39-ROV39ROV/6-1b gill21.511.6569.82
M68/1-39-ROV39ROV/6-1c gill3669H2-Spike
M68/1-39-ROV39ROV/6-1c gill3671164127
M68/1-39-ROV39ROV/6-1c gill21.51not applicable
M68/1-39-ROV39ROV/6-2a gill0H2-Spike
M68/1-39-ROV39ROV/6-2a gill29272
M68/1-39-ROV39ROV/6-2a gill1828868
M68/1-39-ROV39ROV/6-2a gill2948465
M68/1-39-ROV39ROV/6-2a gill4647659
M68/1-39-ROV39ROV/6-2a gill6346853
M68/1-39-ROV39ROV/6-2a gill10095341
M68/1-39-ROV39ROV/6-2a gill17343527
M68/1-39-ROV39ROV/6-2a gill22.512.2191.27
M68/1-39-ROV39ROV/6-2b gill1812H2-Spike
M68/1-39-ROV39ROV/6-2b gill1814255198
M68/1-39-ROV39ROV/6-2b gill1899233181
M68/1-39-ROV39ROV/6-2b gill2324167129
M68/1-39-ROV39ROV/6-2b gill264911891
M68/1-39-ROV39ROV/6-2b gill32995543
M68/1-39-ROV39ROV/6-2b gill22.516.80295.35
M68/1-39-ROV39ROV/6-2c gill3669H2-Spike
M68/1-39-ROV39ROV/6-2c gill3679
M68/1-39-ROV39ROV/6-2c gill3681135105
M68/1-39-ROV39ROV/6-2c gill22.51not applicable
M68/1-39-ROV39ROV/6-3a gill0H2-Spike
M68/1-39-ROV39ROV/6-3a gill29372
M68/1-39-ROV39ROV/6-3a gill928566
M68/1-39-ROV39ROV/6-3a gill1828062
M68/1-39-ROV39ROV/6-3a gill2947457
M68/1-39-ROV39ROV/6-3a gill4645341
M68/1-39-ROV39ROV/6-3a gill6343628
M68/1-39-ROV39ROV/6-3a gill10091512
M68/1-39-ROV39ROV/6-3a gill173432
M68/1-39-ROV39ROV/6-3a gill28.4100.5816.75
M68/1-39-ROV39ROV/6-3b gill1812H2-Spike
M68/1-39-ROV39ROV/6-3b gill1814234182
M68/1-39-ROV39ROV/6-3b gill1899180140
M68/1-39-ROV39ROV/6-3b gill23245543
M68/1-39-ROV39ROV/6-3b gill26491511
M68/1-39-ROV39ROV/6-3b gill329932
M68/1-39-ROV39ROV/6-3b gill28.4102.3184.86
M68/1-39-ROV39ROV/6-3c gill3679H2-Spike
M68/1-39-ROV39ROV/6-3c gill36819271
M68/1-39-ROV39ROV/6-3c gill28.410not applicable
M68/1-39-ROV39ROV/6-3d gill9772H2-Spike
M68/1-39-ROV39ROV/6-3d gill10981489379
M68/1-39-ROV39ROV/6-3d gill12330266206
M68/1-39-ROV39ROV/6-3d gill28.410not applicable
M68/1-39-ROV39ROV/6-5a gill0H2-Spike
M68/1-39-ROV39ROV/6-5a gill29271
M68/1-39-ROV39ROV/6-5a gill928364
M68/1-39-ROV39ROV/6-5a gill1827860
M68/1-39-ROV39ROV/6-5a gill2947155
M68/1-39-ROV39ROV/6-5a gill4645845
M68/1-39-ROV39ROV/6-5a gill6344434
M68/1-39-ROV39ROV/6-5a gill10092217
M68/1-39-ROV39ROV/6-5a gill173475
M68/1-39-ROV39ROV/6-5a gill19.6100.4812.84
M68/1-39-ROV39ROV/6-5b gill1812H2-Spike
M68/1-39-ROV39ROV/6-5b gill1814235182
M68/1-39-ROV39ROV/6-5b gill1899195151
M68/1-39-ROV39ROV/6-5b gill23247961
M68/1-39-ROV39ROV/6-5b gill26492923
M68/1-39-ROV39ROV/6-5b gill329965
M68/1-39-ROV39ROV/6-5b gill19.6101.6960.19
M68/1-39-ROV39ROV/6-5c gill3679H2-Spike
M68/1-39-ROV39ROV/6-5c gill36818566
M68/1-39-ROV39ROV/6-5c gill19.610not applicable
M68/1-39-ROV39ROV/6-5d gill9763H2-Spike
M68/1-39-ROV39ROV/6-5d gill11021601466
M68/1-39-ROV39ROV/6-5d gill12330184143
M68/1-39-ROV39ROV/6-5d gill19.610not applicable
M68/1-39-ROV39ROV/6-10a gill0H2-Spike
M68/1-39-ROV39ROV/6-10a gill29472
M68/1-39-ROV39ROV/6-10a gill928264
M68/1-39-ROV39ROV/6-10a gill1827054
M68/1-39-ROV39ROV/6-10a gill2945744
M68/1-39-ROV39ROV/6-10a gill4644031
M68/1-39-ROV39ROV/6-10a gill6342519
M68/1-39-ROV39ROV/6-10a gill100997
M68/1-39-ROV39ROV/6-10a gill173411
M68/1-39-ROV39ROV/6-10a gill22.8100.9430.89
M68/1-39-ROV39ROV/6-10b gill1812H2-Spike
M68/1-39-ROV39ROV/6-10b gill1814231179
M68/1-39-ROV39ROV/6-10b gill1899176137
M68/1-39-ROV39ROV/6-10b gill23246047
M68/1-39-ROV39ROV/6-10b gill26492015
M68/1-39-ROV39ROV/6-10b gill329954
M68/1-39-ROV39ROV/6-10b gill22.8102.2381.64
M68/1-39-ROV39ROV/6-10c gill3679H2-Spike
M68/1-39-ROV39ROV/6-10c gill36818465
M68/1-39-ROV39ROV/6-10c gill22.810not applicable
M68/1-39-ROV39ROV/6-10d gill9755H2-Spike
M68/1-39-ROV39ROV/6-10d gill11029679526
M68/1-39-ROV39ROV/6-10d gill12330216168
M68/1-39-ROV39ROV/6-10d gill22.810not applicable
M68/1-39-ROV39ROV/6-1a foot0H2-Spike
M68/1-39-ROV39ROV/6-1a foot29473
M68/1-39-ROV39ROV/6-1a foot929271
M68/1-39-ROV39ROV/6-1a foot1828666
M68/1-39-ROV39ROV/6-1a foot2948465
M68/1-39-ROV39ROV/6-1a foot4648364
M68/1-39-ROV39ROV/6-1a foot6348264
M68/1-39-ROV39ROV/6-1a foot10098264
M68/1-39-ROV39ROV/6-1a foot17348062
M68/1-39-ROV39ROV/6-1a foot10.20not applicable
M68/1-39-ROV39ROV/6-1b foot1812H2-Spike
M68/1-39-ROV39ROV/6-1b foot1814291225
M68/1-39-ROV39ROV/6-1b foot1899283220
M68/1-39-ROV39ROV/6-1b foot2324277215
M68/1-39-ROV39ROV/6-1b foot2649275213
M68/1-39-ROV39ROV/6-1b foot3299272211
M68/1-39-ROV39ROV/6-1b foot10.29not applicable
M68/1-39-ROV39ROV/6-1c foot3679H2-Spike
M68/1-39-ROV39ROV/6-1c foot3681316245
M68/1-39-ROV39ROV/6-1c foot1not applicable
M68/1-39-ROV39ROV/6-1d foot9746H2-Spike
M68/1-39-ROV39ROV/6-1d foot1097116041243
M68/1-39-ROV39ROV/6-1d foot1235016331266
M68/1-39-ROV39ROV/6-1d foot1not applicable
M68/1-39-ROV(39ROV/6) seawater control (a)0H2-Spike
M68/1-39-ROV(39ROV/6) seawater control (a)29473
M68/1-39-ROV(39ROV/6) seawater control (a)929372
M68/1-39-ROV(39ROV/6) seawater control (a)1829271
M68/1-39-ROV(39ROV/6) seawater control (a)2948767
M68/1-39-ROV(39ROV/6) seawater control (a)4648767
M68/1-39-ROV(39ROV/6) seawater control (a)6348767
M68/1-39-ROV(39ROV/6) seawater control (a)10098667
M68/1-39-ROV(39ROV/6) seawater control (a)17348364
M68/1-39-ROV(39ROV/6) seawater control (a)0.21not applicable
M68/1-39-ROV(39ROV/6) seawater control (b)1812H2-Spike
M68/1-39-ROV(39ROV/6) seawater control (b)1814158122
M68/1-39-ROV(39ROV/6) seawater control (b)1899156121
M68/1-39-ROV(39ROV/6) seawater control (b)2324152118
M68/1-39-ROV(39ROV/6) seawater control (b)2649143110
M68/1-39-ROV(39ROV/6) seawater control (b)3304150116
M68/1-39-ROV(39ROV/6) seawater control (b)0.18not applicable
M68/1-39-ROV(39ROV/6) seawater control (c)3679H2-Spike
M68/1-39-ROV(39ROV/6) seawater control (c)3681229177
M68/1-39-ROV(39ROV/6) seawater control (c)not applicable
M68/1-39-ROV(39ROV/6) seawater control (d)9737H2-Spike
M68/1-39-ROV(39ROV/6) seawater control (d)1097116621288
M68/1-39-ROV(39ROV/6) seawater control (d)1233016541282
M68/1-39-ROV(39ROV/6) seawater control (d)not applicable
M68/1-70-ROV 70ROV/8-1a gill0H2-Spike
M68/1-70-ROV70ROV/8-1a gill221731684
M68/1-70-ROV70ROV/8-1a gill9220471586
M68/1-70-ROV70ROV/8-1a gill46017451352
M68/1-70-ROV70ROV/8-1a gill84714871153
M68/1-70-ROV70ROV/8-1a gill12971143886
M68/1-70-ROV70ROV/8-1a gill2012776601
M68/1-70-ROV70ROV/8-1a gill2602477369
M68/1-70-ROV70ROV/8-1a gill29.3104.77128.21
M68/1-70-ROV70ROV/8-1b gill2657H2-Spike
M68/1-70-ROV70ROV/8-1b gill266230202340
M68/1-70-ROV70ROV/8-1b gill275225822001
M68/1-70-ROV70ROV/8-1b gill293221901697
M68/1-70-ROV70ROV/8-1b gill331714721141
M68/1-70-ROV70ROV/8-1b gill3677996772
M68/1-70-ROV70ROV/8-1b gill29.31012.51390.04
M68/1-70-ROV70ROV/8-2a gill0H2-Spike
M68/1-70-ROV70ROV/8-2a gill121271648
M68/1-70-ROV70ROV/8-2a gill9120191565
M68/1-70-ROV70ROV/8-2a gill45917001317
M68/1-70-ROV70ROV/8-2a gill84614671137
M68/1-70-ROV70ROV/8-2a gill12961177912
M68/1-70-ROV70ROV/8-2a gill2011759588
M68/1-70-ROV70ROV/8-2a gill2601479371
M68/1-70-ROV70ROV/8-2a gill25.9104.53120.06
M68/1-70-ROV70ROV/8-2b gill2659H2-Spike
M68/1-70-ROV70ROV/8-2b gill266128732226
M68/1-70-ROV70ROV/8-2b gill275125171951
M68/1-70-ROV70ROV/8-2b gill293120271571
M68/1-70-ROV70ROV/8-2b gill331613311032
M68/1-70-ROV70ROV/8-2b gill3676743576
M68/1-70-ROV70ROV/8-2b gill25.91011.48355.17
M68/1-70-ROV70ROV/8-3a gill0H2-Spike
M68/1-70-ROV70ROV/8-3a gill121351655
M68/1-70-ROV70ROV/8-3a gill9120461586
M68/1-70-ROV70ROV/8-3a gill45916951313
M68/1-70-ROV70ROV/8-3a gill84613731064
M68/1-70-ROV70ROV/8-3a gill12961000775
M68/1-70-ROV70ROV/8-3a gill2011653506
M68/1-70-ROV70ROV/8-3a gill2601412319
M68/1-70-ROV70ROV/8-3a gill28.8105.83163.98
M68/1-70-ROV70ROV/8-3b gill2660H2-Spike
M68/1-70-ROV70ROV/8-3b gill266128552212
M68/1-70-ROV70ROV/8-3b gill275126262035
M68/1-70-ROV70ROV/8-3b gill293122611752
M68/1-70-ROV70ROV/8-3b gill331616201255
M68/1-70-ROV70ROV/8-3b gill36761033801
M68/1-70-ROV70ROV/8-3b gill28.8106.94201.54
M68/1-70-ROV70ROV/8-1 foot0H2-Spike
M68/1-70-ROV70ROV/8-1 foot220041553
M68/1-70-ROV70ROV/8-1 foot9219551515
M68/1-70-ROV70ROV/8-1 foot46019511512
M68/1-70-ROV70ROV/8-1 foot84719141483
M68/1-70-ROV70ROV/8-1 foot129718811458
M68/1-70-ROV70ROV/8-1 foot201218691448
M68/1-70-ROV70ROV/8-1 foot260218741452
M68/1-70-ROV70ROV/8-1 foot275218771455
M68/1-70-ROV70ROV/8-1 foot293218621443
M68/1-70-ROV70ROV/8-1 foot331718831459
M68/1-70-ROV70ROV/8-1 foot367718441429
M68/1-70-ROV70ROV/8-1 foot11.26not applicable
M68/1-70-ROV(70ROV/8) seawater control0
M68/1-70-ROV(70ROV/8) seawater control320951623
M68/1-70-ROV(70ROV/8) seawater control9320701604
M68/1-70-ROV(70ROV/8) seawater control46120161562
M68/1-70-ROV(70ROV/8) seawater control84820401581
M68/1-70-ROV(70ROV/8) seawater control129819981548
M68/1-70-ROV(70ROV/8) seawater control201320001550
M68/1-70-ROV(70ROV/8) seawater control260319871540
M68/1-70-ROV(70ROV/8) seawater control275320271571
M68/1-70-ROV(70ROV/8) seawater control293320041553
M68/1-70-ROV(70ROV/8) seawater control331819971548
M68/1-70-ROV(70ROV/8) seawater control367819891541
M68/1-70-ROV(70ROV/8) seawater control0.71not applicable