/* DATA DESCRIPTION: Citation: Petersen, Jillian M; Zielinski, Frank U; Pape, Thomas; Seifert, Richard; Moraru, Cristina; Amann, Rudolf; Hourdez, Stéphane; Girguis, Peter R; Wankel, Scott D; Barbe, Valerie; Pelletier, Eric; Fink, Dennis; Borowski, Christian; Bach, Wolfgang; Dubilier, Nicole (2013): Hydrogen consumption by gills of Bathymodiolus spp. collected at the Comfortless cove and Lilliput hydrothermal vent fields in May 2006 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.808951, In supplement to: Petersen, JM et al. (2011): Hydrogen is an energy source for hydrothermal vent symbioses. Nature, 476, 176-180, https://doi.org/10.1038/nature10325 Abstract: The discovery of deep-sea hydrothermal vents in 1977 revolutionized our understanding of the energy sources that fuel primary productivity on Earth. Hydrothermal vent ecosystems are dominated by animals that live in symbiosis with chemosynthetic bacteria. So far, only two energy sources have been shown to power chemosynthetic symbioses: reduced sulphur compounds and methane. Using metagenome sequencing, single-gene fluorescence in situ hybridization, immunohistochemistry, shipboard incubations and in situ mass spectrometry, we show here that the symbionts of the hydrothermal vent mussel Bathymodiolus from the Mid-Atlantic Ridge use hydrogen to power primary production. In addition, we show that the symbionts of Bathymodiolus mussels from Pacific vents have hupL, the key gene for hydrogen oxidation. Furthermore, the symbionts of other vent animals such as the tubeworm Riftia pachyptila and the shrimp Rimicaris exoculata also have hupL. We propose that the ability to use hydrogen as an energy source is widespread in hydrothermal vent symbioses, particularly at sites where hydrogen is abundant. Related to: Koschinsky, Andrea; Billings, Andrew; Devey, Colin W; Dubilier, Nicole; Duester, Alan; Edge, David; Garbe-Schönberg, Dieter; German, Christopher R; Giere, Olav; Keir, Robin; Lackschewitz, Klas Sven; Mai, Anh Hoang; Marbler, Herwig; Mawick, Jule; Melchert, Bernd; Mertens, Christian; Ochsenhirt, Wolf-Thilo; Peters, Marc; Sander, Sylvia; Schmale, Oliver; Schmidt, Werner; Seifert, Richard; Seiter, Christian; Stöber, Uwe; Suck, Inken; Walter, Maren; Weber, Stefan; Yoerger, Dana; Zarrouk, Marcel; Zielinski, Frank (2006): Mid-Atlantic Expedition 2006, Cruise No. 68, Leg 1, Fluid geochemistry, biology and geological setting of hydrothermal systems at the southern MAR (4°S - 10°S) (MAR-SÜD III), 27 April - 2 June 2006, Bridgetown (Barbados) - Recife (Brazil). METEOR-Berichte, Leitstelle Meteor, Institut für Meereskunde der Universität Hamburg, 06, 189 pp, https://doi.org/10.2312/cr_m68-1 Koschinsky, Andrea; Billings, Andrew; Devey, Colin W; Dubilier, Nicole; Duester, Alan; Edge, David; Garbe-Schönberg, Dieter; German, Christopher R; Giere, Olav; Keir, Robin; Lackschewitz, Klas Sven; Mai, Anh Hoang; Marbler, Herwig; Mawick, Jule; Melchert, Bernd; Mertens, Christian; Peters, Michael; Sander, Sylvia; Schmale, Oliver; Schmidt, Werner; Seifert, Richard; Seiter, Christian; Stöber, Uwe; Suck, Inken; Walter, Maren; Weber, Stefan; Yoerger, Dana; Zarrouk, Marcel; Zielinski, Frank (2006): Discovery of new hydrothermal vent fields on the Southern Mid-Atlantic Ridge (4°S - 10°S) during Cruise M68/1. InterRidge News, 15, 9-15, hdl:10013/epic.41214.d001 Zielinski, Frank (2009): Geobiological coupling of hydrothermal vent fluids with endosymbiotic primary producers of bathymodiolus mussels from hydrothermal vents on the Mid-Atlantic Ridge [dissertation]. University of Bremen, Germany, 159 pp, urn:nbn:de:gbv:46-diss000115375 Project(s): From Mantle to Ocean: Energy-, Material- and Life-cycles at Spreading Axes (DERIDGE) (URI: https://www.geomar.de/forschen/fb4/fb4-muhs/projekte/spp1144/) Funding: German Research Foundation (DFG) (URI: http://www.dfg.de/en/), grant/award no. 5471797: From Mantle to Ocean: Energy-, Material- and Life-cycles at Spreading Axes (URI: https://gepris.dfg.de/project/5471797) Coverage: MEDIAN LATITUDE: -7.176584 * MEDIAN LONGITUDE: -12.791274 * SOUTH-BOUND LATITUDE: -9.553380 * WEST-BOUND LONGITUDE: -13.209130 * NORTH-BOUND LATITUDE: -4.802330 * EAST-BOUND LONGITUDE: -12.371500 DATE/TIME START: 2006-05-14T11:06:00 * DATE/TIME END: 2006-05-27T13:45:00 MINIMUM ELEVATION: -2993.0 m * MAXIMUM ELEVATION: -1475.0 m Event(s): M68/1-20-ROV * LATITUDE START: -4.802830 * LONGITUDE START: -12.372500 * LATITUDE END: -4.802830 * LONGITUDE END: -12.371500 * DATE/TIME: 2006-05-14T11:06:00 * ELEVATION: -2993.0 m * CAMPAIGN: M68/1 (MARSUED3) * BASIS: Meteor (1986) (URI: https://en.wikipedia.org/wiki/RV_Meteor_(1986)) * METHOD/DEVICE: Remote operated vehicle (ROV) * COMMENT: Twin Peaks M68/1-24-ROV * LATITUDE START: -4.802330 * LONGITUDE START: -12.382000 * LATITUDE END: -4.803000 * LONGITUDE END: -12.371500 * DATE/TIME START: 2006-05-15T15:06:00 * DATE/TIME END: 2006-05-16T01:32:00 * ELEVATION START: -2993.0 m * ELEVATION END: -2991.0 m * CAMPAIGN: M68/1 (MARSUED3) * BASIS: Meteor (1986) (URI: https://en.wikipedia.org/wiki/RV_Meteor_(1986)) * METHOD/DEVICE: Remote operated vehicle (ROV) * COMMENT: Turtle Pits area M68/1-39-ROV * LATITUDE START: -9.553380 * LONGITUDE START: -13.208560 * LATITUDE END: -9.548630 * LONGITUDE END: -13.209130 * DATE/TIME START: 2006-05-18T19:24:00 * DATE/TIME END: 2006-05-19T00:01:00 * ELEVATION START: -1475.0 m * ELEVATION END: -1494.0 m * CAMPAIGN: M68/1 (MARSUED3) * BASIS: Meteor (1986) (URI: https://en.wikipedia.org/wiki/RV_Meteor_(1986)) * METHOD/DEVICE: Remote operated vehicle (ROV) * COMMENT: S of Liliput, 2nd mussel bed M68/1-70-ROV * LATITUDE START: -9.550170 * LONGITUDE START: -13.206330 * LATITUDE END: -9.549500 * LONGITUDE END: -13.208670 * DATE/TIME: 2006-05-27T13:45:00 * ELEVATION: -1495.0 m * CAMPAIGN: M68/1 (MARSUED3) * BASIS: Meteor (1986) (URI: https://en.wikipedia.org/wiki/RV_Meteor_(1986)) * METHOD/DEVICE: Remote operated vehicle (ROV) * COMMENT: Liliput area Comment: Hydrogen consumption incubations Sampling site: For on-board physiological experiments specimens belonging to undescribed Bathymodiolus species (B. spp.) were collected at the Comfortless Cove and Lilliput hydrothermal vent fields on the southern Mid-Atlantic Ridge. Mussels were sampled using nets (40 cm length, 20 cm diameter opening, mesh size 335 µm, Hydrobios, Kiel, Germany) handled by the manipulator arm of the remotely operated vehicles (ROVs) Quest 4000 m (MARUM, Bremen, Germany). Mussel dissection for shipboard incubations: Mussels were opened with a scalpel by cutting through the posterior and anterior adductor muscles. Viability was tested by prodding the foot with a dissection needle and only mussels whose foot contracted were used. The foot and both gills were separated from the remaining tissue using dissection scissors. Tissue pieces of 6 mm in diameter were cut out of the gill and the foot tissues using a steel hole-puncher. One tissue piece from each individual was frozen in liquid nitrogen for weight determination in the home laboratory. For negative controls, foot tissues were used which do not contain endosymbiotic bacteria. Experimental setup: For hydrogen partial pressures up to 1,000 ppm (in helium) glass serum vials (58 ml) were fully filled with chilled (4°C) sterile-filtered (0.22 µm) bottom seawater retrieved from 3,000 m water depth. One piece of gill tissue was placed in the vial, the vial closed with a gas-tight butyl rubber stopper, and crimped with aluminium seals. Control vials contained foot tissue or no tissue at all. 20 ml of hydrogen gas (100 ppm, 250 ppm or 1,000 ppm in helium, Linde) were injected through the rubber stopper using a gas-tight syringe (SGE Analytical Science and Hamilton) with a second syringe allowing pressure compensation through the outflow of the same volume of seawater. For hydrogen partial pressures above 1,000 ppm (in air) glass serum vials were processed as described above with the exception that the vials were filled with only 38 ml of seawater. Pure hydrogen gas (100%; Air Liquide) was then injected in the air headspace through the rubber stopper to the desired final partial pressure (1,000-3,000 ppm) using a gas-tight syringe. All vials were placed upside down to minimize possible gas exchange via the rubber stopper and incubated at 4°C on a slowly rotating table. At given time points a subsample was taken with a gas-tight syringe from the headspace with the pressure decrease compensated through the inflow of oxygen-saturated, chilled sterile-filtered seawater from a second syringe. Analysis of the headspace hydrogen content: The hydrogen concentration in the headspace was determined using a gas chromatograph (Thermo Trace GC ultra) equipped with a packed stainless steel column (Molecular Sieve 5A, carrier gas: He) and a pulse discharge detector (PDD). Recording and calculation of results was performed using a PC operated integration system (Thermo Chrom Card A/D). Analytical procedures were calibrated daily with commercial gas standards (Linde). Incubation conditions and rate calculations: The concentrations of dissolved hydrogen and oxygen were calculated from Crozier and Yamamoto (1974) [doi:10.1021/je60062a007] and Weiss (1970) [doi:10.1016/0011-7471(70)90037-9] under the assumption of Henry's law i.e. that the concentration of a dissolved gas in a solution is directly proportional to the partial pressure of that gas above the solution. The molar volume of an ideal gas (22.414 l mol-1) was used to convert between the partial pressure of a gas (ppm) and the amount of the gas (in moles) in the headspace. The rates of hydrogen removal from the headspace [in nmol h-1 (piece tissue)-1] were calculated for the first 60 minutes after addition of the electron source performing non-linear regression through the data points obtained during the complete incubation period of up to 60 hours. Exponential and hyperbolic regression described by the equations f=a*exp(b/(x+c)) and f=y0+(a*b)/(b+x) gave well-fitting regression curves (R2 = 0.920 and 0.921, respectively; standard error of estimate 6.434 and 6.400, respectively). The effect of methodological hydrogen removal from the headspace through subsampling was considered. The rates at which hydrogen disappeared from incubation vials containing only seawater but no tissues (chemical oxidation, hydrogen loss by diffusion) were subtracted from the tissue rates. Resulting rates were then normalized to gram wet weight (in nmol h-1 [g wet weight]-1). Parameter(s): Event label (Event) Sample ID (Sample ID) * PI: Zielinski, Frank (frank.zielinski@ufz.de) Time in minutes [min] (Time min) * PI: Zielinski, Frank (frank.zielinski@ufz.de) Hydrogen [ppmv] (H2) * PI: Zielinski, Frank (frank.zielinski@ufz.de) * COMMENT: headspace Hydrogen concentration [nmol] (H2) * PI: Zielinski, Frank (frank.zielinski@ufz.de) * COMMENT: dissolved Wet mass [mg] (Wet m) * PI: Zielinski, Frank (frank.zielinski@ufz.de) Tissue piece, number of [#] (Tissue p) * PI: Zielinski, Frank (frank.zielinski@ufz.de) Hydrogen consumption rate [nmol/h] (H2 cons rate) * PI: Zielinski, Frank (frank.zielinski@ufz.de) * COMMENT: per gill tissue piece Hydrogen consumption rate per weight [nmol/h/g] (H2 cons rate) * PI: Zielinski, Frank (frank.zielinski@ufz.de) * COMMENT: normalized to wet weight Comment (Comment) * PI: Zielinski, Frank (frank.zielinski@ufz.de) License: Creative Commons Attribution 3.0 Unported (CC-BY-3.0) (URI: https://creativecommons.org/licenses/by/3.0/) Size: 1582 data points */ Event Sample ID Time min [min] H2 [ppmv] H2 [nmol] Wet m [mg] Tissue p [#] H2 cons rate [nmol/h] H2 cons rate [nmol/h/g] Comment M68/1-20-ROV 20ROV/1-1 gill 0 H2-Spike M68/1-20-ROV 20ROV/1-1 gill 4 95 73 M68/1-20-ROV 20ROV/1-1 gill 104 91 71 M68/1-20-ROV 20ROV/1-1 gill 244 87 68 M68/1-20-ROV 20ROV/1-1 gill 424 84 65 M68/1-20-ROV 20ROV/1-1 gill 664 80 62 M68/1-20-ROV 20ROV/1-1 gill 964 74 57 M68/1-20-ROV 20ROV/1-1 gill 1698 72 55 M68/1-20-ROV 20ROV/1-1 gill 2303 66 51 M68/1-20-ROV 20ROV/1-1 gill 3883 49 38 M68/1-20-ROV 20ROV/1-1 gill 5358 28 22 M68/1-20-ROV 20ROV/1-1 gill 38.4 1 0.62 12.23 M68/1-20-ROV 20ROV/1-2 gill 0 H2-Spike M68/1-20-ROV 20ROV/1-2 gill 2 93 72 M68/1-20-ROV 20ROV/1-2 gill 242 84 65 M68/1-20-ROV 20ROV/1-2 gill 422 76 59 M68/1-20-ROV 20ROV/1-2 gill 662 72 56 M68/1-20-ROV 20ROV/1-2 gill 972 70 54 M68/1-20-ROV 20ROV/1-2 gill 1696 57 44 M68/1-20-ROV 20ROV/1-2 gill 2301 45 35 M68/1-20-ROV 20ROV/1-2 gill 3881 31 24 M68/1-20-ROV 20ROV/1-2 gill 5356 20 15 M68/1-20-ROV 20ROV/1-2 gill 36.0 1 1.85 47.07 M68/1-20-ROV 20ROV/1-1 foot 0 H2-Spike M68/1-20-ROV 20ROV/1-1 foot 2 97 75 M68/1-20-ROV 20ROV/1-1 foot 102 96 74 M68/1-20-ROV 20ROV/1-1 foot 242 93 72 M68/1-20-ROV 20ROV/1-1 foot 422 91 70 M68/1-20-ROV 20ROV/1-1 foot 662 91 70 M68/1-20-ROV 20ROV/1-1 foot 972 90 69 M68/1-20-ROV 20ROV/1-1 foot 1696 88 68 M68/1-20-ROV 20ROV/1-1 foot 2301 86 67 M68/1-20-ROV 20ROV/1-1 foot 3881 85 66 M68/1-20-ROV 20ROV/1-1 foot 5356 80 62 M68/1-20-ROV 20ROV/1-1 foot 1 0.10 not applicable M68/1-20-ROV (20ROV/1) seawater control 0 H2-Spike M68/1-20-ROV (20ROV/1) seawater control 2 96 74 M68/1-20-ROV (20ROV/1) seawater control 102 91 71 M68/1-20-ROV (20ROV/1) seawater control 242 92 71 M68/1-20-ROV (20ROV/1) seawater control 422 90 69 M68/1-20-ROV (20ROV/1) seawater control 662 91 70 M68/1-20-ROV (20ROV/1) seawater control 972 90 70 M68/1-20-ROV (20ROV/1) seawater control 1696 90 70 M68/1-20-ROV (20ROV/1) seawater control 2301 87 68 M68/1-20-ROV (20ROV/1) seawater control 3881 85 66 M68/1-20-ROV (20ROV/1) seawater control 5356 82 63 M68/1-20-ROV (20ROV/1) seawater control 0.07 not applicable M68/1-20-ROV 20ROV/1-1 gill 0 H2-Spike M68/1-20-ROV 20ROV/1-1 gill 2 938 727 M68/1-20-ROV 20ROV/1-1 gill 102 902 699 M68/1-20-ROV 20ROV/1-1 gill 242 891 690 M68/1-20-ROV 20ROV/1-1 gill 422 843 654 M68/1-20-ROV 20ROV/1-1 gill 662 837 649 M68/1-20-ROV 20ROV/1-1 gill 972 810 627 M68/1-20-ROV 20ROV/1-1 gill 1696 747 579 M68/1-20-ROV 20ROV/1-1 gill 2301 670 519 M68/1-20-ROV 20ROV/1-1 gill 3881 429 332 M68/1-20-ROV 20ROV/1-1 gill 5356 325 251 M68/1-20-ROV 20ROV/1-1 gill 38.4 1 5.27 4.29 M68/1-20-ROV 20ROV/1-2 gill 0 H2-Spike M68/1-20-ROV 20ROV/1-2 gill 2 949 735 M68/1-20-ROV 20ROV/1-2 gill 102 905 702 M68/1-20-ROV 20ROV/1-2 gill 242 892 691 M68/1-20-ROV 20ROV/1-2 gill 422 824 639 M68/1-20-ROV 20ROV/1-2 gill 662 787 610 M68/1-20-ROV 20ROV/1-2 gill 972 735 570 M68/1-20-ROV 20ROV/1-2 gill 1696 652 505 M68/1-20-ROV 20ROV/1-2 gill 2301 560 434 M68/1-20-ROV 20ROV/1-2 gill 3881 362 281 M68/1-20-ROV 20ROV/1-2 gill 5356 299 232 M68/1-20-ROV 20ROV/1-2 gill 36.0 1 11.42 10.44 M68/1-20-ROV 20ROV/1-1 foot 0 H2-Spike M68/1-20-ROV 20ROV/1-1 foot 2 966 748 M68/1-20-ROV 20ROV/1-1 foot 102 910 705 M68/1-20-ROV 20ROV/1-1 foot 242 906 702 M68/1-20-ROV 20ROV/1-1 foot 422 905 702 M68/1-20-ROV 20ROV/1-1 foot 662 905 701 M68/1-20-ROV 20ROV/1-1 foot 972 904 700 M68/1-20-ROV 20ROV/1-1 foot 1696 898 696 M68/1-20-ROV 20ROV/1-1 foot 2301 880 682 M68/1-20-ROV 20ROV/1-1 foot 3881 847 657 M68/1-20-ROV 20ROV/1-1 foot 5356 800 620 M68/1-20-ROV 20ROV/1-1 foot 1 0.63 not applicable M68/1-20-ROV (20ROV/1) seawater control 0 H2-Spike M68/1-20-ROV (20ROV/1) seawater control 2 987 765 M68/1-20-ROV (20ROV/1) seawater control 102 936 725 M68/1-20-ROV (20ROV/1) seawater control 242 938 727 M68/1-20-ROV (20ROV/1) seawater control 422 932 722 M68/1-20-ROV (20ROV/1) seawater control 662 927 718 M68/1-20-ROV (20ROV/1) seawater control 972 910 705 M68/1-20-ROV (20ROV/1) seawater control 1696 830 643 M68/1-20-ROV (20ROV/1) seawater control 2301 834 646 M68/1-20-ROV (20ROV/1) seawater control 3881 820 635 M68/1-20-ROV (20ROV/1) seawater control 5356 795 616 M68/1-20-ROV (20ROV/1) seawater control 1.09 not applicable M68/1-24-ROV 24ROV/5-1 gill 0 H2-Spike M68/1-24-ROV 24ROV/5-1 gill 2 926 718 M68/1-24-ROV 24ROV/5-1 gill 92 920 713 M68/1-24-ROV 24ROV/5-1 gill 282 881 683 M68/1-24-ROV 24ROV/5-1 gill 477 849 658 M68/1-24-ROV 24ROV/5-1 gill 707 820 636 M68/1-24-ROV 24ROV/5-1 gill 1052 782 606 M68/1-24-ROV 24ROV/5-1 gill 2242 674 522 M68/1-24-ROV 24ROV/5-1 gill 3762 579 448 M68/1-24-ROV 24ROV/5-1 gill 5407 445 345 M68/1-24-ROV 24ROV/5-1 gill 24.0 1 6.97 249.67 M68/1-24-ROV 24ROV/5-2a gill 0 H2-Spike M68/1-24-ROV 24ROV/5-2a gill 2 972 753 M68/1-24-ROV 24ROV/5-2a gill 92 938 727 M68/1-24-ROV 24ROV/5-2a gill 282 888 689 M68/1-24-ROV 24ROV/5-2a gill 497 867 672 M68/1-24-ROV 24ROV/5-2a gill 727 793 614 M68/1-24-ROV 24ROV/5-2a gill 1072 806 625 M68/1-24-ROV 24ROV/5-2a gill 2262 725 562 M68/1-24-ROV 24ROV/5-2a gill 3782 569 441 M68/1-24-ROV 24ROV/5-2a gill 5427 424 328 M68/1-24-ROV 24ROV/5-2a gill 30.3 1 6.94 196.65 M68/1-24-ROV 24ROV/5-2b gill 0 H2-Spike M68/1-24-ROV 24ROV/5-2b gill 2 822 637 M68/1-24-ROV 24ROV/5-2b gill 92 821 636 M68/1-24-ROV 24ROV/5-2b gill 102 793 614 M68/1-24-ROV 24ROV/5-2b gill 272 772 598 M68/1-24-ROV 24ROV/5-2b gill 477 758 587 M68/1-24-ROV 24ROV/5-2b gill 707 749 580 M68/1-24-ROV 24ROV/5-2b gill 1062 707 548 M68/1-24-ROV 24ROV/5-2b gill 2292 650 504 M68/1-24-ROV 24ROV/5-2b gill 3762 617 478 M68/1-24-ROV 24ROV/5-2b gill 5407 567 439 M68/1-24-ROV 24ROV/5-2b gill 30.3 1 6.74 190.19 M68/1-24-ROV 24ROV/5-1a foot 0 H2-Spike M68/1-24-ROV 24ROV/5-1a foot 2 978 758 M68/1-24-ROV 24ROV/5-1a foot 92 950 736 M68/1-24-ROV 24ROV/5-1a foot 282 931 721 M68/1-24-ROV 24ROV/5-1a foot 487 903 700 M68/1-24-ROV 24ROV/5-1a foot 717 889 689 M68/1-24-ROV 24ROV/5-1a foot 1062 896 694 M68/1-24-ROV 24ROV/5-1a foot 2252 838 650 M68/1-24-ROV 24ROV/5-1a foot 3772 810 627 M68/1-24-ROV 24ROV/5-1a foot 5417 770 597 M68/1-24-ROV 24ROV/5-1a foot 1 1.19 not applicable M68/1-24-ROV 24ROV/5-1b foot 0 H2-Spike M68/1-24-ROV 24ROV/5-1b foot 2 904 701 M68/1-24-ROV 24ROV/5-1b foot 102 797 617 M68/1-24-ROV 24ROV/5-1b foot 272 805 624 M68/1-24-ROV 24ROV/5-1b foot 477 797 617 M68/1-24-ROV 24ROV/5-1b foot 707 807 626 M68/1-24-ROV 24ROV/5-1b foot 1062 783 607 M68/1-24-ROV 24ROV/5-1b foot 2292 762 591 M68/1-24-ROV 24ROV/5-1b foot 3762 756 586 M68/1-24-ROV 24ROV/5-1b foot 5407 649 503 M68/1-24-ROV 24ROV/5-1b foot 1 1.01 not applicable M68/1-24-ROV (24ROV/5) seawater control A 0 H2-Spike M68/1-24-ROV (24ROV/5) seawater control A 2 983 762 M68/1-24-ROV (24ROV/5) seawater control A 92 936 725 M68/1-24-ROV (24ROV/5) seawater control A 282 919 712 M68/1-24-ROV (24ROV/5) seawater control A 487 923 716 M68/1-24-ROV (24ROV/5) seawater control A 717 916 710 M68/1-24-ROV (24ROV/5) seawater control A 1062 904 700 M68/1-24-ROV (24ROV/5) seawater control A 2252 877 680 M68/1-24-ROV (24ROV/5) seawater control A 3772 848 657 M68/1-24-ROV (24ROV/5) seawater control A 5417 798 618 M68/1-24-ROV (24ROV/5) seawater control A 0.88 not applicable M68/1-24-ROV (24ROV/5) seawater control B 0 H2-Spike M68/1-24-ROV (24ROV/5) seawater control B 2 893 692 M68/1-24-ROV (24ROV/5) seawater control B 102 880 682 M68/1-24-ROV (24ROV/5) seawater control B 272 867 672 M68/1-24-ROV (24ROV/5) seawater control B 477 878 680 M68/1-24-ROV (24ROV/5) seawater control B 707 873 677 M68/1-24-ROV (24ROV/5) seawater control B 1062 857 664 M68/1-24-ROV (24ROV/5) seawater control B 2292 826 640 M68/1-24-ROV (24ROV/5) seawater control B 3762 806 624 M68/1-24-ROV (24ROV/5) seawater control B 5407 708 549 M68/1-24-ROV (24ROV/5) seawater control B 1.07 not applicable M68/1-39-ROV 39ROV/6-1a gill 0 H2-Spike M68/1-39-ROV 39ROV/6-1a gill 2 94 73 M68/1-39-ROV 39ROV/6-1a gill 92 88 68 M68/1-39-ROV 39ROV/6-1a gill 182 89 69 M68/1-39-ROV 39ROV/6-1a gill 294 83 65 M68/1-39-ROV 39ROV/6-1a gill 634 80 62 M68/1-39-ROV 39ROV/6-1a gill 979 74 57 M68/1-39-ROV 39ROV/6-1a gill 1734 69 53 M68/1-39-ROV 39ROV/6-1a gill 21.5 1 1.65 69.75 M68/1-39-ROV 39ROV/6-1b gill 1812 H2-Spike M68/1-39-ROV 39ROV/6-1b gill 1814 136 105 M68/1-39-ROV 39ROV/6-1b gill 1899 127 99 M68/1-39-ROV 39ROV/6-1b gill 2324 113 88 M68/1-39-ROV 39ROV/6-1b gill 2649 100 78 M68/1-39-ROV 39ROV/6-1b gill 3299 82 63 M68/1-39-ROV 39ROV/6-1b gill 21.5 1 1.65 69.82 M68/1-39-ROV 39ROV/6-1c gill 3669 H2-Spike M68/1-39-ROV 39ROV/6-1c gill 3671 164 127 M68/1-39-ROV 39ROV/6-1c gill 21.5 1 not applicable M68/1-39-ROV 39ROV/6-2a gill 0 H2-Spike M68/1-39-ROV 39ROV/6-2a gill 2 92 72 M68/1-39-ROV 39ROV/6-2a gill 182 88 68 M68/1-39-ROV 39ROV/6-2a gill 294 84 65 M68/1-39-ROV 39ROV/6-2a gill 464 76 59 M68/1-39-ROV 39ROV/6-2a gill 634 68 53 M68/1-39-ROV 39ROV/6-2a gill 1009 53 41 M68/1-39-ROV 39ROV/6-2a gill 1734 35 27 M68/1-39-ROV 39ROV/6-2a gill 22.5 1 2.21 91.27 M68/1-39-ROV 39ROV/6-2b gill 1812 H2-Spike M68/1-39-ROV 39ROV/6-2b gill 1814 255 198 M68/1-39-ROV 39ROV/6-2b gill 1899 233 181 M68/1-39-ROV 39ROV/6-2b gill 2324 167 129 M68/1-39-ROV 39ROV/6-2b gill 2649 118 91 M68/1-39-ROV 39ROV/6-2b gill 3299 55 43 M68/1-39-ROV 39ROV/6-2b gill 22.5 1 6.80 295.35 M68/1-39-ROV 39ROV/6-2c gill 3669 H2-Spike M68/1-39-ROV 39ROV/6-2c gill 3679 M68/1-39-ROV 39ROV/6-2c gill 3681 135 105 M68/1-39-ROV 39ROV/6-2c gill 22.5 1 not applicable M68/1-39-ROV 39ROV/6-3a gill 0 H2-Spike M68/1-39-ROV 39ROV/6-3a gill 2 93 72 M68/1-39-ROV 39ROV/6-3a gill 92 85 66 M68/1-39-ROV 39ROV/6-3a gill 182 80 62 M68/1-39-ROV 39ROV/6-3a gill 294 74 57 M68/1-39-ROV 39ROV/6-3a gill 464 53 41 M68/1-39-ROV 39ROV/6-3a gill 634 36 28 M68/1-39-ROV 39ROV/6-3a gill 1009 15 12 M68/1-39-ROV 39ROV/6-3a gill 1734 3 2 M68/1-39-ROV 39ROV/6-3a gill 28.4 10 0.58 16.75 M68/1-39-ROV 39ROV/6-3b gill 1812 H2-Spike M68/1-39-ROV 39ROV/6-3b gill 1814 234 182 M68/1-39-ROV 39ROV/6-3b gill 1899 180 140 M68/1-39-ROV 39ROV/6-3b gill 2324 55 43 M68/1-39-ROV 39ROV/6-3b gill 2649 15 11 M68/1-39-ROV 39ROV/6-3b gill 3299 3 2 M68/1-39-ROV 39ROV/6-3b gill 28.4 10 2.31 84.86 M68/1-39-ROV 39ROV/6-3c gill 3679 H2-Spike M68/1-39-ROV 39ROV/6-3c gill 3681 92 71 M68/1-39-ROV 39ROV/6-3c gill 28.4 10 not applicable M68/1-39-ROV 39ROV/6-3d gill 9772 H2-Spike M68/1-39-ROV 39ROV/6-3d gill 10981 489 379 M68/1-39-ROV 39ROV/6-3d gill 12330 266 206 M68/1-39-ROV 39ROV/6-3d gill 28.4 10 not applicable M68/1-39-ROV 39ROV/6-5a gill 0 H2-Spike M68/1-39-ROV 39ROV/6-5a gill 2 92 71 M68/1-39-ROV 39ROV/6-5a gill 92 83 64 M68/1-39-ROV 39ROV/6-5a gill 182 78 60 M68/1-39-ROV 39ROV/6-5a gill 294 71 55 M68/1-39-ROV 39ROV/6-5a gill 464 58 45 M68/1-39-ROV 39ROV/6-5a gill 634 44 34 M68/1-39-ROV 39ROV/6-5a gill 1009 22 17 M68/1-39-ROV 39ROV/6-5a gill 1734 7 5 M68/1-39-ROV 39ROV/6-5a gill 19.6 10 0.48 12.84 M68/1-39-ROV 39ROV/6-5b gill 1812 H2-Spike M68/1-39-ROV 39ROV/6-5b gill 1814 235 182 M68/1-39-ROV 39ROV/6-5b gill 1899 195 151 M68/1-39-ROV 39ROV/6-5b gill 2324 79 61 M68/1-39-ROV 39ROV/6-5b gill 2649 29 23 M68/1-39-ROV 39ROV/6-5b gill 3299 6 5 M68/1-39-ROV 39ROV/6-5b gill 19.6 10 1.69 60.19 M68/1-39-ROV 39ROV/6-5c gill 3679 H2-Spike M68/1-39-ROV 39ROV/6-5c gill 3681 85 66 M68/1-39-ROV 39ROV/6-5c gill 19.6 10 not applicable M68/1-39-ROV 39ROV/6-5d gill 9763 H2-Spike M68/1-39-ROV 39ROV/6-5d gill 11021 601 466 M68/1-39-ROV 39ROV/6-5d gill 12330 184 143 M68/1-39-ROV 39ROV/6-5d gill 19.6 10 not applicable M68/1-39-ROV 39ROV/6-10a gill 0 H2-Spike M68/1-39-ROV 39ROV/6-10a gill 2 94 72 M68/1-39-ROV 39ROV/6-10a gill 92 82 64 M68/1-39-ROV 39ROV/6-10a gill 182 70 54 M68/1-39-ROV 39ROV/6-10a gill 294 57 44 M68/1-39-ROV 39ROV/6-10a gill 464 40 31 M68/1-39-ROV 39ROV/6-10a gill 634 25 19 M68/1-39-ROV 39ROV/6-10a gill 1009 9 7 M68/1-39-ROV 39ROV/6-10a gill 1734 1 1 M68/1-39-ROV 39ROV/6-10a gill 22.8 10 0.94 30.89 M68/1-39-ROV 39ROV/6-10b gill 1812 H2-Spike M68/1-39-ROV 39ROV/6-10b gill 1814 231 179 M68/1-39-ROV 39ROV/6-10b gill 1899 176 137 M68/1-39-ROV 39ROV/6-10b gill 2324 60 47 M68/1-39-ROV 39ROV/6-10b gill 2649 20 15 M68/1-39-ROV 39ROV/6-10b gill 3299 5 4 M68/1-39-ROV 39ROV/6-10b gill 22.8 10 2.23 81.64 M68/1-39-ROV 39ROV/6-10c gill 3679 H2-Spike M68/1-39-ROV 39ROV/6-10c gill 3681 84 65 M68/1-39-ROV 39ROV/6-10c gill 22.8 10 not applicable M68/1-39-ROV 39ROV/6-10d gill 9755 H2-Spike M68/1-39-ROV 39ROV/6-10d gill 11029 679 526 M68/1-39-ROV 39ROV/6-10d gill 12330 216 168 M68/1-39-ROV 39ROV/6-10d gill 22.8 10 not applicable M68/1-39-ROV 39ROV/6-1a foot 0 H2-Spike M68/1-39-ROV 39ROV/6-1a foot 2 94 73 M68/1-39-ROV 39ROV/6-1a foot 92 92 71 M68/1-39-ROV 39ROV/6-1a foot 182 86 66 M68/1-39-ROV 39ROV/6-1a foot 294 84 65 M68/1-39-ROV 39ROV/6-1a foot 464 83 64 M68/1-39-ROV 39ROV/6-1a foot 634 82 64 M68/1-39-ROV 39ROV/6-1a foot 1009 82 64 M68/1-39-ROV 39ROV/6-1a foot 1734 80 62 M68/1-39-ROV 39ROV/6-1a foot 1 0.20 not applicable M68/1-39-ROV 39ROV/6-1b foot 1812 H2-Spike M68/1-39-ROV 39ROV/6-1b foot 1814 291 225 M68/1-39-ROV 39ROV/6-1b foot 1899 283 220 M68/1-39-ROV 39ROV/6-1b foot 2324 277 215 M68/1-39-ROV 39ROV/6-1b foot 2649 275 213 M68/1-39-ROV 39ROV/6-1b foot 3299 272 211 M68/1-39-ROV 39ROV/6-1b foot 1 0.29 not applicable M68/1-39-ROV 39ROV/6-1c foot 3679 H2-Spike M68/1-39-ROV 39ROV/6-1c foot 3681 316 245 M68/1-39-ROV 39ROV/6-1c foot 1 not applicable M68/1-39-ROV 39ROV/6-1d foot 9746 H2-Spike M68/1-39-ROV 39ROV/6-1d foot 10971 1604 1243 M68/1-39-ROV 39ROV/6-1d foot 12350 1633 1266 M68/1-39-ROV 39ROV/6-1d foot 1 not applicable M68/1-39-ROV (39ROV/6) seawater control (a) 0 H2-Spike M68/1-39-ROV (39ROV/6) seawater control (a) 2 94 73 M68/1-39-ROV (39ROV/6) seawater control (a) 92 93 72 M68/1-39-ROV (39ROV/6) seawater control (a) 182 92 71 M68/1-39-ROV (39ROV/6) seawater control (a) 294 87 67 M68/1-39-ROV (39ROV/6) seawater control (a) 464 87 67 M68/1-39-ROV (39ROV/6) seawater control (a) 634 87 67 M68/1-39-ROV (39ROV/6) seawater control (a) 1009 86 67 M68/1-39-ROV (39ROV/6) seawater control (a) 1734 83 64 M68/1-39-ROV (39ROV/6) seawater control (a) 0.21 not applicable M68/1-39-ROV (39ROV/6) seawater control (b) 1812 H2-Spike M68/1-39-ROV (39ROV/6) seawater control (b) 1814 158 122 M68/1-39-ROV (39ROV/6) seawater control (b) 1899 156 121 M68/1-39-ROV (39ROV/6) seawater control (b) 2324 152 118 M68/1-39-ROV (39ROV/6) seawater control (b) 2649 143 110 M68/1-39-ROV (39ROV/6) seawater control (b) 3304 150 116 M68/1-39-ROV (39ROV/6) seawater control (b) 0.18 not applicable M68/1-39-ROV (39ROV/6) seawater control (c) 3679 H2-Spike M68/1-39-ROV (39ROV/6) seawater control (c) 3681 229 177 M68/1-39-ROV (39ROV/6) seawater control (c) not applicable M68/1-39-ROV (39ROV/6) seawater control (d) 9737 H2-Spike M68/1-39-ROV (39ROV/6) seawater control (d) 10971 1662 1288 M68/1-39-ROV (39ROV/6) seawater control (d) 12330 1654 1282 M68/1-39-ROV (39ROV/6) seawater control (d) not applicable M68/1-70-ROV 70ROV/8-1a gill 0 H2-Spike M68/1-70-ROV 70ROV/8-1a gill 2 2173 1684 M68/1-70-ROV 70ROV/8-1a gill 92 2047 1586 M68/1-70-ROV 70ROV/8-1a gill 460 1745 1352 M68/1-70-ROV 70ROV/8-1a gill 847 1487 1153 M68/1-70-ROV 70ROV/8-1a gill 1297 1143 886 M68/1-70-ROV 70ROV/8-1a gill 2012 776 601 M68/1-70-ROV 70ROV/8-1a gill 2602 477 369 M68/1-70-ROV 70ROV/8-1a gill 29.3 10 4.77 128.21 M68/1-70-ROV 70ROV/8-1b gill 2657 H2-Spike M68/1-70-ROV 70ROV/8-1b gill 2662 3020 2340 M68/1-70-ROV 70ROV/8-1b gill 2752 2582 2001 M68/1-70-ROV 70ROV/8-1b gill 2932 2190 1697 M68/1-70-ROV 70ROV/8-1b gill 3317 1472 1141 M68/1-70-ROV 70ROV/8-1b gill 3677 996 772 M68/1-70-ROV 70ROV/8-1b gill 29.3 10 12.51 390.04 M68/1-70-ROV 70ROV/8-2a gill 0 H2-Spike M68/1-70-ROV 70ROV/8-2a gill 1 2127 1648 M68/1-70-ROV 70ROV/8-2a gill 91 2019 1565 M68/1-70-ROV 70ROV/8-2a gill 459 1700 1317 M68/1-70-ROV 70ROV/8-2a gill 846 1467 1137 M68/1-70-ROV 70ROV/8-2a gill 1296 1177 912 M68/1-70-ROV 70ROV/8-2a gill 2011 759 588 M68/1-70-ROV 70ROV/8-2a gill 2601 479 371 M68/1-70-ROV 70ROV/8-2a gill 25.9 10 4.53 120.06 M68/1-70-ROV 70ROV/8-2b gill 2659 H2-Spike M68/1-70-ROV 70ROV/8-2b gill 2661 2873 2226 M68/1-70-ROV 70ROV/8-2b gill 2751 2517 1951 M68/1-70-ROV 70ROV/8-2b gill 2931 2027 1571 M68/1-70-ROV 70ROV/8-2b gill 3316 1331 1032 M68/1-70-ROV 70ROV/8-2b gill 3676 743 576 M68/1-70-ROV 70ROV/8-2b gill 25.9 10 11.48 355.17 M68/1-70-ROV 70ROV/8-3a gill 0 H2-Spike M68/1-70-ROV 70ROV/8-3a gill 1 2135 1655 M68/1-70-ROV 70ROV/8-3a gill 91 2046 1586 M68/1-70-ROV 70ROV/8-3a gill 459 1695 1313 M68/1-70-ROV 70ROV/8-3a gill 846 1373 1064 M68/1-70-ROV 70ROV/8-3a gill 1296 1000 775 M68/1-70-ROV 70ROV/8-3a gill 2011 653 506 M68/1-70-ROV 70ROV/8-3a gill 2601 412 319 M68/1-70-ROV 70ROV/8-3a gill 28.8 10 5.83 163.98 M68/1-70-ROV 70ROV/8-3b gill 2660 H2-Spike M68/1-70-ROV 70ROV/8-3b gill 2661 2855 2212 M68/1-70-ROV 70ROV/8-3b gill 2751 2626 2035 M68/1-70-ROV 70ROV/8-3b gill 2931 2261 1752 M68/1-70-ROV 70ROV/8-3b gill 3316 1620 1255 M68/1-70-ROV 70ROV/8-3b gill 3676 1033 801 M68/1-70-ROV 70ROV/8-3b gill 28.8 10 6.94 201.54 M68/1-70-ROV 70ROV/8-1 foot 0 H2-Spike M68/1-70-ROV 70ROV/8-1 foot 2 2004 1553 M68/1-70-ROV 70ROV/8-1 foot 92 1955 1515 M68/1-70-ROV 70ROV/8-1 foot 460 1951 1512 M68/1-70-ROV 70ROV/8-1 foot 847 1914 1483 M68/1-70-ROV 70ROV/8-1 foot 1297 1881 1458 M68/1-70-ROV 70ROV/8-1 foot 2012 1869 1448 M68/1-70-ROV 70ROV/8-1 foot 2602 1874 1452 M68/1-70-ROV 70ROV/8-1 foot 2752 1877 1455 M68/1-70-ROV 70ROV/8-1 foot 2932 1862 1443 M68/1-70-ROV 70ROV/8-1 foot 3317 1883 1459 M68/1-70-ROV 70ROV/8-1 foot 3677 1844 1429 M68/1-70-ROV 70ROV/8-1 foot 1 1.26 not applicable M68/1-70-ROV (70ROV/8) seawater control 0 M68/1-70-ROV (70ROV/8) seawater control 3 2095 1623 M68/1-70-ROV (70ROV/8) seawater control 93 2070 1604 M68/1-70-ROV (70ROV/8) seawater control 461 2016 1562 M68/1-70-ROV (70ROV/8) seawater control 848 2040 1581 M68/1-70-ROV (70ROV/8) seawater control 1298 1998 1548 M68/1-70-ROV (70ROV/8) seawater control 2013 2000 1550 M68/1-70-ROV (70ROV/8) seawater control 2603 1987 1540 M68/1-70-ROV (70ROV/8) seawater control 2753 2027 1571 M68/1-70-ROV (70ROV/8) seawater control 2933 2004 1553 M68/1-70-ROV (70ROV/8) seawater control 3318 1997 1548 M68/1-70-ROV (70ROV/8) seawater control 3678 1989 1541 M68/1-70-ROV (70ROV/8) seawater control 0.71 not applicable