Schilling, Marcel; Maiwald, Thomas; Hengl, Stefan; Winter, Dominic; Kreutz, Clemens; Kolch, Walter; Lehmann, Wolf D; Timmer, Jens; Klingmüller, Ursula: Modeled integrated responses of ppERK1 and ppERK2 of normal and elevated ERK1 and ERK2 levels (Figure 5b) [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.775546 (unpublished dataset)
Abstract:
The present dataset contain source data for Figure 5b from Schilling et al., 2009. Cell fate decisions are regulated by the coordinated activation of signalling pathways such as the extracellular signal-regulated kinase (ERK) cascade, but contributions of individual kinase isoforms are mostly unknown. The authors combined quantitative data from erythropoietin-induced pathway activation in primary erythroid progenitor (colony-forming unit erythroid stage, CFU-E) cells with mathematical modelling, in order to predict and experimentally confirmed a distributive ERK phosphorylation mechanism in CFU-E cells. The authors found evidences that double-phosphorylated ERK1 attenuates proliferation beyond a certain activation level, whereas activated ERK2 enhances proliferation with saturation kinetics. They show integrated responses of double-phosphorylated ERK1 and ERK2 that were calculated for different Epo concentrations for the original model as well as for models with elevated ERK1 or ERK2 levels.
Related to:
Schilling, Marcel; Maiwald, Thomas; Hengl, Stefan; Winter, Dominic; Kreutz, Clemens; Kolch, Walter; Lehmann, Wolf D; Timmer, Jens; Klingmüller, Ursula (2009): Theoretical and experimental analysis links isoformspecific ERK signalling to cell fate decisions. Molecular Systems Biology, 5, https://doi.org/10.1038/msb.2009.91
Coverage:
Date/Time Start: 2009-01-01T00:00:00 * Date/Time End: 2009-01-01T00:00:00
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | ||||
| 2 | Treatment: Erythropoietin | T:Epo | U/ml | Schilling, Marcel | Chemical used as treatment; [UniProtKB: P29676] (http://identifiers.org/uniprot/P29676) | |
| 3 | Treatment: Amount concentration, of Extracellular signal-Regulated Kinase 1 | T:ERK1 | Schilling, Marcel | Chemical used as treatment i.e. elevated or control | ||
| 4 | Treatment: Amount concentration, of Extracellular signal-Regulated Kinase 2 | T:ERK2 | Schilling, Marcel | Chemical used as treatment i.e. elevated or control | ||
| 5 | Response (time*molecule concentration in cells) of double-phosphorylated Extracellular signal-Regulated Kinase 1, computed | ppERK1 computed | min*104 molec/# | Schilling, Marcel | Result of integrated response to different treatments, computed | |
| 6 | Response (time*molecule concentration in cells) of double-phosphorylated Extracellular signal-Regulated Kinase 2, computed | ppERK2 computed | min*104 molec/# | Schilling, Marcel | Result of integrated response to different treatments, computed |
Size:
225 data points