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Schilling, Marcel; Maiwald, Thomas; Hengl, Stefan; Winter, Dominic; Kreutz, Clemens; Kolch, Walter; Lehmann, Wolf D; Timmer, Jens; Klingmüller, Ursula (2012): Proliferation of retrovirally transduced CFU-E cells after incubation with increasing Epo-concentration (Figure 5c). PANGAEA, https://doi.org/10.1594/PANGAEA.775548

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Abstract:
Data contain source data for Figure 5c from Schilling et al., 2009. Cell fate decisions are regulated by the coordinated activation of signalling pathways such as the extracellular signal-regulated kinase (ERK) cascade, but contributions of individual kinase isoforms are mostly unknown. The authors combined quantitative data from erythropoietin-induced pathway activation in primary erythroid progenitor (colony-forming unit erythroid stage, CFU-E) cells with mathematical modelling, in order to predict and experimentally confirmed a distributive ERK phosphorylation mechanism in CFU-E cells. The authors found evidences that double-phosphorylated ERK1 attenuates proliferation beyond a certain activation level, whereas activated ERK2 enhances proliferation with saturation kinetics. Retrovirally transduced CFU-E cells were incubated with increasing Epo concentrations for 14 h and proliferation was measured by [3H]-thymidine incorporation.
Related to:
Schilling, Marcel; Maiwald, Thomas; Hengl, Stefan; Winter, Dominic; Kreutz, Clemens; Kolch, Walter; Lehmann, Wolf D; Timmer, Jens; Klingmüller, Ursula (2009): Theoretical and experimental analysis links isoformspecific ERK signalling to cell fate decisions. Molecular Systems Biology, 5, https://doi.org/10.1038/msb.2009.91
Coverage:
Date/Time Start: 2007-11-26T00:00:00 * Date/Time End: 2007-11-26T00:00:00
Event(s):
Experiment_ppERK_Treatment_a * Date/Time: 2007-11-26T00:00:00 * Comment: TREATMENT: ERK1=control; ERK2=control; ARK=control
Experiment_ppERK_Treatment_b * Date/Time: 2007-11-26T00:00:00 * Comment: TREATMENT: ERK1=elevated; ERK2=control; ARK=control
Experiment_ppERK_Treatment_c * Date/Time: 2007-11-26T00:00:00 * Comment: TREATMENT: ERK1=control; ERK2=elevated; ARK=control
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Event labelEvent
2Treatment: chemical concentration (biological activity), of ErythropoietinT:EpoU/mlSchilling, MarcelChemical used as treatment; [UniProtKB: P29676] (http://identifiers.org/uniprot/P29676)
3Treatment: Amount concentration, of Extracellular signal-Regulated Kinase 1T:ERK1Schilling, MarcelChemical used as treatment i.e. elevated or control
4Treatment: Amount concentration, of Extracellular signal-Regulated Kinase 2T:ERK2Schilling, MarcelChemical used as treatment i.e. elevated or control
5Treatment: Amount concentration, protein kinase BT:AKTSchilling, MarcelChemical used as treatment i.e. elevated or control
6Detector raw countsCountscpmSchilling, MarcelThymidine incorporation
Size:
800 data points

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