Schilling, Marcel; Maiwald, Thomas; Hengl, Stefan; Winter, Dominic; Kreutz, Clemens; Kolch, Walter; Lehmann, Wolf D; Timmer, Jens; Klingmüller, Ursula (2012): Proliferation of retrovirally transduced CFU-E cells after incubation with increasing Epo-concentration (Figure 5c) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.775548
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Abstract:
Data contain source data for Figure 5c from Schilling et al., 2009. Cell fate decisions are regulated by the coordinated activation of signalling pathways such as the extracellular signal-regulated kinase (ERK) cascade, but contributions of individual kinase isoforms are mostly unknown. The authors combined quantitative data from erythropoietin-induced pathway activation in primary erythroid progenitor (colony-forming unit erythroid stage, CFU-E) cells with mathematical modelling, in order to predict and experimentally confirmed a distributive ERK phosphorylation mechanism in CFU-E cells. The authors found evidences that double-phosphorylated ERK1 attenuates proliferation beyond a certain activation level, whereas activated ERK2 enhances proliferation with saturation kinetics. Retrovirally transduced CFU-E cells were incubated with increasing Epo concentrations for 14 h and proliferation was measured by [3H]-thymidine incorporation.
Related to:
Schilling, Marcel; Maiwald, Thomas; Hengl, Stefan; Winter, Dominic; Kreutz, Clemens; Kolch, Walter; Lehmann, Wolf D; Timmer, Jens; Klingmüller, Ursula (2009): Theoretical and experimental analysis links isoformspecific ERK signalling to cell fate decisions. Molecular Systems Biology, 5, https://doi.org/10.1038/msb.2009.91
Coverage:
Date/Time Start: 2007-11-26T00:00:00 * Date/Time End: 2007-11-26T00:00:00
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | ||||
| 2 | Treatment: chemical concentration (biological activity), of Erythropoietin | T:Epo | U/ml | Schilling, Marcel | Chemical used as treatment; [UniProtKB: P29676] (http://identifiers.org/uniprot/P29676) | |
| 3 | Treatment: Amount concentration, of Extracellular signal-Regulated Kinase 1 | T:ERK1 | Schilling, Marcel | Chemical used as treatment i.e. elevated or control | ||
| 4 | Treatment: Amount concentration, of Extracellular signal-Regulated Kinase 2 | T:ERK2 | Schilling, Marcel | Chemical used as treatment i.e. elevated or control | ||
| 5 | Treatment: Amount concentration, protein kinase B | T:AKT | Schilling, Marcel | Chemical used as treatment i.e. elevated or control | ||
| 6 | Detector raw counts | Counts | cpm | Schilling, Marcel | Thymidine incorporation |
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
800 data points