Lorenzo, Rodrigo; Romero, Carolina; Tapella, Federico; Storch, Daniela: Haemolymph lactate, muscle lactate, midgut gland lactate and midgut gland glycogen of Lithodes santolla exposed to different oxygen and temperature conditions measured in 2017 in the laboratory in Ushuaia, Argentina [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.997826 (dataset in review)
Abstract:
This dataset comprises physiological and metabolic measurements of the southern king crab, Lithodes santolla, exposed to different temperatures (0–25 ºC) and two oxygen conditions (Normoxia: 21 kPa and Reduction: 12 kPa). Metabolic variables include lactate and glycogen concentrations measured in haemolymph, muscle, and midgut gland tissues. The dataset was generated to evaluate the physiological responses of L. santolla to environmental variation in temperature and oxygen availability. Metabolic measurements were obtained in 2017 in Ushuaia, Tierra del Fuego, Argentina, using animals purchased from local fishers. Lactate concentrations were determined using a commercial enzymatic assay kit (Wiener Lab, Rosario, Argentina), and glycogen concentrations were quantified using the phenol-sulfuric acid method following Lo et al. (1970). Absorbance measurements were performed with a microplate reader (BioTek Instruments, Winooski, USA).
Keyword(s):
Supplement to:
Lorenzo, Rodrigo; Romero, Carolina; Tapella, Federico; Storch, Daniela (submitted): Moderate reduction in ambient oxygen increases thermal sensitivity of Lithodes santolla.
Related to:
Lorenzo, Rodrigo; Romero, Carolina; Tapella, Federico; Storch, Daniela: Oxygen consumption, heart rate, and ventilation rate of Lithodes santolla in response to declining oxygen partial pressures measured in 2016 in the laboratory in Germany [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.997822
Lorenzo, Rodrigo; Romero, Carolina; Tapella, Federico; Storch, Daniela: Oxygen consumption, heart rate, and ventilation rate of Lithodes santolla in response to different temperatures and oxygen levels measured in 2016 in the laboratory in Germany [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.997816
References:
Lo, S; Russell, J C; Taylor, A W (1970): Determination of glycogen in small tissue samples. Journal of Applied Physiology, 28(2), 234-236, https://doi.org/10.1152/jappl.1970.28.2.234
Project(s):
Funding:
Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i), grant/award no. PICT 12-2368: Agencia Nacional de Promoción Científica y Tecnológica of Argentina
German Research Foundation (DFG), grant/award no. 238009887: Climate driven Antarctic invasion? Physiological impacts of temperature and hypoxia on life stages of reptant decapod crustaceans and implications for distribution shifts
Horizon 2020 of the European Commission (H2020), grant/award no. 872690: Coastal ecosystem carbon balance in times of rapid glacier melt (CoastCarb)
Coverage:
Median Latitude: -54.810500 * Median Longitude: -68.299000 * South-bound Latitude: -54.822000 * West-bound Longitude: -68.322000 * North-bound Latitude: -54.799000 * East-bound Longitude: -68.276000
Date/Time Start: 2017-03-28T17:00:00 * Date/Time End: 2017-03-28T17:00:00
Event(s):
Catch_L_santolla_2017 (Purchase of Lithodes santolla from local fishermen for experiments) * Latitude: -54.799000 * Longitude: -68.276000 * Date/Time Start: 2017-01-15T10:00:00 * Date/Time End: 2017-01-15T11:00:00 * Location: Beagle Channel * Method/Device: Fishing pods
Metabolite_by_temperature (Laboratory experiment in Ushuaia, Argentina on the effect of oxygen availability and temperature on the metabolism of Lithodes santolla) * Latitude: -54.822000 * Longitude: -68.322000 * Date/Time: 2017-03-28T17:00:00 * Location: Ushuaia, Argentina * Method/Device: Laboratory experiment
Comment:
Further funding:
* Program-oriented Funding IV (PoF IV), Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research
Parameter(s):
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1492 data points