Thielecke, Antonia; Eggers, Sarah Lena; Fernández-Méndez, Mar: Carbon uptake rates in water and ice samples during Polarstern cruise ArcWatch-1 (PS138) in 2023 [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.996486 (dataset in review)
Abstract:
Net primary production (NPP) and photosynthesis–irradiance (PI) parameters were determined from water column and sea-ice samples collected during the ArcWatch-1 (PS138) expedition aboard RV Polarstern (3 August – 30 September 2023). Water samples were collected from the surface and the Chl a maximum using a CTD-rosette (SBE 911+, Sea-Bird Electronics); sea ice was sampled with a Kovacs corer and under-ice water (UIW) and melt pond water (MPW) was sampled with a hand pump from directly beneath the ice and the meltponds, respectively. Ice cores were cut into a bottom section of 10 cm and the remaining top section. Upper core sections were mechanically broken to accelerate melting and pooled. NPP was measured via the ¹⁴C uptake method (Steemann Nielsen, 1952): samples were spiked with NaH¹⁴CO₃ (1 µCi mL⁻¹) and incubated for 8 h at 0°C across a light gradient (0–430 µmol photons m⁻² s⁻¹, spherical quantum sensor). Particulate ¹⁴C uptake was quantified by liquid scintillation counting after filtration (0.2 µm), with dark uptake subtracted. Dissolved inorganic carbon samples were collected in triplicates and were measured by flow injection analysis (Hall and Aller, 1992) to calculate isotope incorporation. Chlorophyll a was quantified by reverse-phase HPLC (modified from Barlow et al., 1997). PI curves were fitted using the Platt et al. (1980) model (nonlinear least-squares, R package minpack.lm), yielding maximum photosynthetic capacity, initial slope, and photoinhibition parameter, with derived light-response parameters calculated following Platt et al. (1982).
Related to:
Barlow, Raymond G; Cummings, Denise G; Gibb, S W (1997): Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC. Marine Ecology Progress Series, 161, 303-307, https://doi.org/10.3354/meps161303
Hall, Per O J; Aller, Robert C (1992): Rapid, small-volume, flow injection analysis for SCO2, and NH4+ in marine and freshwaters. Limnology and Oceanography, 37(5), 1113-1119, https://doi.org/10.4319/lo.1992.37.5.1113
Nielsen, E S (1952): The Use of Radio-active Carbon (C14) for Measuring Organic Production in the Sea. ICES Journal of Marine Science, 18(2), 117-140, https://doi.org/10.1093/icesjms/18.2.117
Platt, Trevor; Gallegos, Charles L; Harrison, W G (1980): Photoinhibition of Photosynthesis in Natural Assemblages of Marine Phytoplankton. Journal of Marine Research, 38, 687-701
Platt, Trevor; Harrison, W G; Irwin, Brian; Horne, Edward; Gallegos, Charles L (1982): Photosynthesis and photoadaptation of marine phytoplankton in the arctic. Deep Sea Research Part A. Oceanographic Research Papers, 29(10), 1159-1170, https://doi.org/10.1016/0198-0149(82)90087-5
Project(s):
Funding:
Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven (AWI), grant/award no. AWI_PS138_02: Biological Oceanography
Coverage:
Median Latitude: 86.082443 * Median Longitude: 71.922694 * South-bound Latitude: 80.949072 * West-bound Longitude: -15.222563 * North-bound Latitude: 89.935866 * East-bound Longitude: 130.350341
Date/Time Start: 2023-08-06T15:38:56 * Date/Time End: 2023-09-20T08:57:11
Minimum DEPTH, water: 2.0 m * Maximum DEPTH, water: 53.0 m
Event(s):
PS138_2-1 * Latitude: 80.948096 * Longitude: 15.350650 * Date/Time: 2023-08-06T15:38:56 * Elevation: -2028.0 m * O2A Registry URI: https://registry.o2a-data.de/items/5452 * Campaign: PS138 (ArcWatch-1) * Basis: Polarstern * Method/Device: CTD/Rosette (CTD-RO) * Comment: at max depth
PS138_9_Coring-001 (SI_corer_9cm) * Latitude: 84.073565 * Longitude: 31.211554 * Date/Time Start: 2023-08-08T16:53:13 * Date/Time End: 2023-08-08T16:53:13 * O2A Registry URI: https://registry.o2a-data.de/items/5065 * Campaign: PS138 (ArcWatch-1) * Basis: Polarstern * Method/Device: Mark II motorized coring device, Kovacs Enterprise Inc (MARKII) * Comment: Biocore_1; IceStation #1
PS138_9_Coring-002 (SI_corer_9cm) * Latitude: 84.073565 * Longitude: 31.211554 * Date/Time Start: 2023-08-08T16:53:33 * Date/Time End: 2023-08-08T16:53:33 * O2A Registry URI: https://registry.o2a-data.de/items/5065 * Campaign: PS138 (ArcWatch-1) * Basis: Polarstern * Method/Device: Mark II motorized coring device, Kovacs Enterprise Inc (MARKII) * Comment: Biocore_2; IceStation #1
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Thielecke, Antonia | |||
| 2 | Type of study | Study type | Thielecke, Antonia | |||
| 3 | Sample type | Samp type | Thielecke, Antonia | |||
| 4 | Core section, comment | Sec comm | Thielecke, Antonia | ice core IDs of pooled cores, top and bottom sections, combined and used for biological analysis | ||
| 5 | LATITUDE | Latitude | Thielecke, Antonia | Geocode | ||
| 6 | LONGITUDE | Longitude | Thielecke, Antonia | Geocode | ||
| 7 | DATE/TIME | Date/Time | Thielecke, Antonia | Geocode | ||
| 8 | Treatment: light intensity | T:Io | µmol/m2/s | Thielecke, Antonia | ||
| 9 | Ice-station | Ice-station | Thielecke, Antonia | |||
| 10 | Incubation temperature | Inc Temp | °C | Thielecke, Antonia | ||
| 11 | Carbon, inorganic, dissolved | DIC | mmol/l | Thielecke, Antonia | Flow injection analysis (Hall and Aller 1992) | |
| 12 | Carbon, inorganic, dissolved, standard deviation | DIC std dev | ± | Thielecke, Antonia | Flow injection analysis (Hall and Aller 1992) | |
| 13 | Incubation duration | Inc dur | days | Thielecke, Antonia | ||
| 14 | Carbon uptake rate, fractionated | C upt rate frac | µg/l/h | Thielecke, Antonia | 14C radiotracer incubation | |
| 15 | Chlorophyll a | Chl a | µg/l | Thielecke, Antonia | High Performance Liquid Chromatography (HPLC) | |
| 16 | Initial slope of the photosynthesis-irradiance curve | alpha | (mg C /mg Chl a/h)/(µE/m2/s) | Thielecke, Antonia | PGH model fit according to Platt et al. (1980) and nonlinear least-square via R minpack.lm | |
| 17 | Photoinhibition parameter | beta | mg C/m3/h/(µmol photons/m2/s) | Thielecke, Antonia | PGH model fit according to Platt et al. (1980) and nonlinear least-square via R minpack.lm | |
| 18 | Light saturated maximum photosynthetic rate per Chlorophyll a | Pbmax | mg/mg/h | Thielecke, Antonia | PGH model fit according to Platt et al. (1980) and nonlinear least-square via R minpack.lm | no photoinhibition correction |
| 19 | Light saturated maximum photosynthetic rate per Chlorophyll a | Pbmax | mg/mg/h | Thielecke, Antonia | Pbmax = Pbmax (uncorr.) x (alpha/(alpha+beta)) x (beta/(alpha+beta))^(beta/alpha) | |
| 20 | Light saturation index | Ik | µmol/m2/s | Thielecke, Antonia | derived, Ik = Pbmax(corr)/alpha | |
| 21 | Irradiance at maximum photosynthetic activity | Im | µmol/m2/s | Thielecke, Antonia | derived, Iopt = (Pbmax(uncorr.)/alpha)*ln((alpha+beta)/beta) | |
| 22 | Coefficient of determination | R**2 | Thielecke, Antonia | PGH model fit according to Platt et al. (1980) and nonlinear least-square via R minpack.lm | ||
| 23 | DEPTH, water | Depth water | m | Thielecke, Antonia | CTD/Rosette (CTD-RO) | Geocode |
| 24 | Ice thickness | Ice thick | m | Thielecke, Antonia | ||
| 25 | Depth, ice/snow, bottom/maximum | Depth ice/snow bot | m | Thielecke, Antonia |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
11634 data points
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