Akeerath Mundanatt, Aman; Vidal-Melgosa, Silvia; Müller, Juliane: Antibody BAM1 binding signals and deoxyhexose concentrations in anion exchange fractions of glycans extracted from sediment cores PS097/72-2 and PS097/72-1 [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.996225 (dataset in review)
Abstract:
Sediment sections from cores PS097/72-2 and PS97/072-1 collected on the expedition PS97 were freeze-dried and homogenized. Glycans were sequentially extracted from the freeze-dried sediment as described by Hellige et al., 2026. Simultaneously, replicates of combusted sand were treated as the negative controls. The extracts were pooled and loaded onto an anion exchange column to isolate anionic glycans. By applying an increasing salt gradient, the extracted glycans were fractionated into 30 x 1 mL AEX fractions. The anion exchange fractions were probed for the presence of fucoidan with monoclonal antibody (BAM1; Torode et al., 2015) using enzyme-linked immunosorbent assay (Hellige et al., 2026). Additionally the deoxyhexose concentrations of the fractions were also quantified using a modified L-cysteine assay (manuscript in prep, Dische and Shettles, 1948). The deoxyhexose concentrations were normalized to the weight of dried sediment.
References:
Dische, Zacharias; Shettles, Landrum B (1948): A SPECIFIC COLOR REACTION OF METHYLPENTOSES AND A SPECTROPHOTOMETRIC MICROMETHOD FOR THEIR DETERMINATION. Journal of Biological Chemistry, 175(2), 595-603, https://doi.org/10.1016/S0021-9258(18)57178-7
Hellige, Inga; Akeerath Mundanatt, Aman; Massing, Jana Chiara; Hehemann, Jan-Hendrik (2026): Fucoidan carbon is stored in coastal vegetated ecosystems. Biogeosciences, 23(1), 387-398, https://doi.org/10.5194/bg-23-387-2026
Torode, Thomas A; Marcus, Susan E; Jam, Murielle; Tonon, Thierry; Blackburn, Richard S; Hervé, Cécile; Knox, J Paul (2015): Monoclonal Antibodies Directed to Fucoidan Preparations from Brown Algae. PLoS ONE, 10(2), e0118366, https://doi.org/10.1371/journal.pone.0118366
Funding:
European Research Council (ERC), grant/award no. 101044738: Discover molecular pathways for marine glyco-Carbon sequestration
Coverage:
Median Latitude: -62.006500 * Median Longitude: -56.064534 * South-bound Latitude: -62.006500 * West-bound Longitude: -56.064670 * North-bound Latitude: -62.006500 * East-bound Longitude: -56.064330
Date/Time Start: 2016-03-10T04:13:00 * Date/Time End: 2016-03-10T06:05:00
Minimum DEPTH, sediment/rock: 0.02 m * Maximum DEPTH, sediment/rock: 8.68 m
Event(s):
PS97/072-1 * Latitude: -62.006500 * Longitude: -56.064330 * Date/Time: 2016-03-10T04:13:00 * Elevation: -1992.9 m * Recovery: 15.83 m * Location: Scotia Sea * Campaign: PS97 (ANT-XXXI/3) * Basis: Polarstern * Method/Device: Piston corer (PC)
PS97/072-2 * Latitude: -62.006500 * Longitude: -56.064670 * Date/Time Start: 2016-03-10T06:05:00 * Date/Time End: 2016-03-10T06:05:00 * Elevation: -1991.7 m * Location: Bransfield Strait * Campaign: PS97 (ANT-XXXI/3) * Basis: Polarstern * Method/Device: MultiCorer (MUC)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Akeerath Mundanatt, Aman | |||
| 2 | LATITUDE | Latitude | Akeerath Mundanatt, Aman | Geocode | ||
| 3 | LONGITUDE | Longitude | Akeerath Mundanatt, Aman | Geocode | ||
| 4 | DATE/TIME | Date/Time | Akeerath Mundanatt, Aman | Geocode | ||
| 5 | DEPTH, sediment/rock | Depth sed | m | Akeerath Mundanatt, Aman | Geocode | |
| 6 | Sample type | Samp type | Akeerath Mundanatt, Aman | |||
| 7 | Anion exchange fraction number | AEX fraction no | Akeerath Mundanatt, Aman | see abstract | Chromatographic fractionation | |
| 8 | Fucoidan, BAM1 antibody signal | Fucoidan BAM1 | Akeerath Mundanatt, Aman | Enzyme-linked immunosorbent assay (ELISA) | OD 450 nm | |
| 9 | Deoxyhexose, per unit sediment dry mass | Deoxyhex/sed dm | µg/g | Akeerath Mundanatt, Aman | L-Cysteine assay |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1567 data points