Trebec, Aja; Hernández-Hernández, Nauzet; Arístegui, Javier: Nutrient limitation study on marine microbial abundance and photophysiology in coastal and offshore waters of the Canary region [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.996055 (dataset in review)
Abstract:
The waters of the Canary Islands region undergo chronic nutrient limitation, resulting in oligotrophy and predominance of picoplankton throughout the year. Situated between the North Atlantic Subtropical Gyre (NASTG) and the highly productive Northwest African coastal upwelling system, and influenced by the continuous Saharan dust deposition, this region experiences a uniquely transient oceanographic regime. To understand nutrient limitation and the potential for nutrient co-limitation in the region, full-factorial nitrate (NO3-), phosphate (PO43-), and dissolved iron (Fe) enrichment bioassay experiments were conducted in coastal and offshore waters east of Gran Canaria in late September 2024. The response of marine microbes to these nutrient additions was measured after 48 hours of incubation. The quantified variables include concentrations of inorganic nutrients (nitrate + nitrite, phosphate and silicate), dissolved organic carbon and nitrogen, total dissolved iron, as well as marine bacterial and phytoplankton abundance and community composition. Additionally, high-resolution photophysiological responses – including maximum photochemical efficiency (Fv/Fm) – were monitored. Measurements were obtained using flow cytometry and a Single Turnover Active Fluorometer (LabSTAF) instrument for bulk photophysiology. This dataset provides key empirical evidence of primary nitrogen limitation, with minimal secondary phosphate limitation, and the absence of iron limitation in the region, offering a distinct contrast to the broader NASTG and advancing our understanding of oligotrophic marine systems such as the Canary Islands region.
Keyword(s):
Related to:
Trebec, Aja; Hernández-Hernández, Nauzet; Arístegui, Javier (submitted): Nitrogen, not iron, controls marine microbial communities in coastal and offshore waters of the Canary region. Scientific Reports
Funding:
Universidad de Las Palmas de Gran Canaria (ULPGC), grant/award no. UPP25/2/T03S: ULPGC 2025 Predoctoral fellowship under the research training program
Coverage:
Median Latitude: 27.984600 * Median Longitude: -15.360900 * South-bound Latitude: 27.979900 * West-bound Longitude: -15.369800 * North-bound Latitude: 27.989300 * East-bound Longitude: -15.352000
Date/Time Start: 2024-09-24T00:00:00 * Date/Time End: 2024-09-25T07:30:00
Minimum DEPTH, water, experiment: 1.5 m * Maximum DEPTH, water, experiment: 1.5 m
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Arístegui, Javier | |||
| 2 | Type of study | Study type | Arístegui, Javier | |||
| 3 | LATITUDE | Latitude | Arístegui, Javier | Geocode | ||
| 4 | LONGITUDE | Longitude | Arístegui, Javier | Geocode | ||
| 5 | Location type | Loc type | Arístegui, Javier | |||
| 6 | DEPTH, water, experiment | Depth water exp | m | Arístegui, Javier | Geocode | |
| 7 | Depth, water, experiment, bottom/maximum | Depth water exp max | m | Arístegui, Javier | Echosounder (ECHO) | |
| 8 | Date/time start, experiment | Date/time start exp | Arístegui, Javier | |||
| 9 | Date/time end, experiment | Date/time end exp | Arístegui, Javier | |||
| 10 | Day of experiment | DOE | day | Arístegui, Javier | ||
| 11 | Experimental treatment | Exp treat | Arístegui, Javier | |||
| 12 | Replicate, biological | Repl bio | Arístegui, Javier | |||
| 13 | Temperature, water | Temp | °C | Arístegui, Javier | Thermometer | |
| 14 | Nitrate and Nitrite | [NO3]- + [NO2]- | µmol/l | Arístegui, Javier | Autoanalyzer (AAIII HR Seal Analytical) according to Aminot (2009) | |
| 15 | Phosphate | [PO4]3- | µmol/l | Arístegui, Javier | Autoanalyzer (AAIII HR Seal Analytical) according to Aminot (2009) | |
| 16 | Silicate | Si(OH)4 | µmol/l | Arístegui, Javier | Autoanalyzer (AAIII HR Seal Analytical) according to Aminot (2009) | |
| 17 | Iron, total | Fe tot | nmol/l | Arístegui, Javier | Element analysis water via ICP-MS (<0.45 µm filtrate) | |
| 18 | Carbon, organic, dissolved | DOC | µmol/l | Arístegui, Javier | Shimadzu TOC-V CSH total organic carbon analyzer coupled to TNM-1 nitrogen analyzer | |
| 19 | Nitrogen, organic, dissolved | DON | µmol/l | Arístegui, Javier | Shimadzu TOC-V CSH total organic carbon analyzer coupled to TNM-1 nitrogen analyzer | |
| 20 | Bacteria, low DNA | Bac low | #/ml | Arístegui, Javier | Flow cytometer, Beckman Coulter, CytoFLEX S | |
| 21 | Bacteria, high DNA | Bac high | #/ml | Arístegui, Javier | Flow cytometer, Beckman Coulter, CytoFLEX S | |
| 22 | Bacteria, heterotrophic | HBA | #/ml | Arístegui, Javier | Flow cytometer, Beckman Coulter, CytoFLEX S | |
| 23 | Prochlorococcus | Prochlorococcus | #/ml | Arístegui, Javier | Flow cytometer, Beckman Coulter, CytoFLEX S | |
| 24 | Synechococcus | Synechococcus | #/ml | Arístegui, Javier | Flow cytometer, Beckman Coulter, CytoFLEX S | |
| 25 | Picoeukaryotes | PEuk | #/ml | Arístegui, Javier | Flow cytometer, Beckman Coulter, CytoFLEX S | |
| 26 | Nanoeukaryotes | NEuk | #/ml | Arístegui, Javier | Flow cytometry | CytoBuoy, CytoSense |
| 27 | Diatoms | Diatoms | #/ml | Arístegui, Javier | ||
| 28 | Maximum photochemical quantum yield of photosystem II | Fv/Fm | Arístegui, Javier | Fluorometer, Chelsea Instruments | LabSTAF | |
| 29 | Fluorescence, minimum | Fo | Arístegui, Javier | Fluorometer, Chelsea Instruments | LabSTAF | |
| 30 | Synechococcus cells, side scatter | Synechococcus SSC | arbitrary units | Arístegui, Javier | Flow cytometer, Beckman Coulter, CytoFLEX S | |
| 31 | Synechococcus cells, chlorophyll, mean fluorescence | Synechococcus chl mean F | arbitrary units | Arístegui, Javier | Flow cytometer, Beckman Coulter, CytoFLEX S | |
| 32 | Synechococcus cells, phycoerythrin, mean fluorescence | Synechococcus phycoe | arbitrary units | Arístegui, Javier | Flow cytometer, Beckman Coulter, CytoFLEX S | |
| 33 | Picophytoplankton cells, side scatter | Picophytopl SSC | arbitrary units | Arístegui, Javier | Flow cytometer, Beckman Coulter, CytoFLEX S; CytoBuoy, CytoSense | |
| 34 | Picophytoplankton cells, chlorophyll, mean fluorescence | Picophytopl chl mean F | arbitrary units | Arístegui, Javier | Flow cytometer, Beckman Coulter, CytoFLEX S; CytoBuoy, CytoSense | |
| 35 | Chlorophyll, mean fluorescence | Chl mean F | arbitrary units | Arístegui, Javier | Flow cytometer, Beckman Coulter, CytoFLEX S; CytoBuoy, CytoSense |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2640 data points
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