Citation:

PANGAEA.995770 (DOI registration in progress)

Name: Behavioral, metabolomic, heat shock protein 70, and catalase responses of Littorina littorea to acute experimental warming in Helgoland (North Sea, Germany) during November 2023 and June 2024
Published: 2026-07-27
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Behavioral response; Gastropod; Laboratory experiment; metabolomics; oxidative stress; Seasonal acclimatization; warming

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Published: 2026-07-27

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Abstract:

Adult Littorina littorea were collected from the intertidal zone of Helgoland (North Sea, Germany; 54°11′ N, 7°53′ E) during November 2023 and June 2024 to investigate physiological and behavioral responses to acute warming. After collection, the organisms were transported to the Alfred Wegener Institute in Bremerhaven, Germany, and acclimated for two weeks to season-specific temperatures representative of winter (10 °C) and summer (16 °C) in aquarium systems containing filtered, recirculating, and continuously aerated seawater at a salinity of 33. Animals were fed Fucus vesiculosus ad libitum throughout the acclimation period. Following acclimation, individuals were exposed to a continuous warming ramp from the respective seasonal acclimation temperature to 34 °C at a rate of 1.5 °C h⁻¹. Samples were collected at predefined temperature intervals between 10 and 34 °C for winter-acclimated animals and between 16 and 34 °C for summer-acclimated animals.The dataset contains behavioral, metabolomic, and biochemical measurements obtained from snails at the respective experimental temperatures. Behavioral observations include the time required for antenna extension and the initiation of locomotion. Metabolomic data were obtained from whole-body soft-tissue extracts using proton nuclear magnetic resonance spectroscopy using the ultra-shielded vertical 9.4 T NMR spectrometer (Advance III HD 400 WB, Bruker-BioSpin GmbH, Germany), with a 1.7 mm triple tuned 1H-13C-15N NMR probe and a Carr-Purcell-Meiboom-Gill sequence (Bruker protocol cpmgpr1d, TOPSPIN 3.5, Bruker GmbH, Germany) with water suppression. Biochemical measurements comprise heat shock protein 70 abundance and catalase activity determined from whole-body tissue homogenates. The data were collected to characterize temperature-dependent physiological and behavioral responses of L. littorea across seasons and to identify thermal thresholds during acute warming exposure.

Keyword(s):

Behavioral response; Gastropod; Laboratory experiment; metabolomics; oxidative stress; Seasonal acclimatization; warming

Related to:

García, Tomás; Bedulina, Daria S; Sokolova, Inna A; Lannig, Gisela (2026): Seasonal impact on the thermal tolerance window of a keystone coastal grazer, the common periwinkle Littorina littorea. Journal of Comparative Physiology B-Biochemical Systemic and Environmentalphysiology, https://doi.org/10.1007/s00360-026-01670-3

Coverage:

Median Latitude: 53.861063 * Median Longitude: 8.231806 * South-bound Latitude: 53.533250 * West-bound Longitude: 7.883131 * North-bound Latitude: 54.188876 * East-bound Longitude: 8.580480

Date/Time Start: 2023-11-03T12:00:00 * Date/Time End: 2024-06-27T07:00:00

Event(s):

Littorina_littorea_exp_summer * Latitude: 53.533250 * Longitude: 8.580480 * Date/Time: 2024-06-27T07:00:00 * Method/Device: Laboratory experiment * Comment: Experimental analyse at the Alfred Wegner Institute Bremerhaven

Littorina_littorea_exp_winter * Latitude: 53.533250 * Longitude: 8.580480 * Date/Time: 2024-04-09T07:00:00 * Method/Device: Laboratory experiment * Comment: Experimental analyse at the Alfred Wegner Institute Bremerhaven

Littorina_littorea_sampling_summer * Latitude: 54.188876 * Longitude: 7.883131 * Date/Time: 2024-06-04T12:00:00 * Method/Device: Biological sample (BIOS) * Comment: Gastropods of the species Littorina littorea were collected during low tide in the Nordstrand, Helgoland by foot during low tide

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		García, Tomás
2	Type of study	Study type		García, Tomás
3	Species, unique identification	Species UID		García, Tomás
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		García, Tomás
5	Species, unique identification (URI)	Species UID (URI)		García, Tomás
6	Sample ID	Sample ID		García, Tomás
7	Treatment: temperature	T:temp	°C	García, Tomás	Fiber-optic thermometer, Weidmann Optocon, FOTEMP1-4
8	Time in seconds	Time sec	s	García, Tomás	Body camera, Hoestr, A39	Time to show antennas
9	Time in seconds	Time sec	s	García, Tomás	Body camera, Hoestr, A39	Start of movement
10	Aminobutyrate	Aminobutyrate	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
11	Adenylates, total	Adenylates	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
12	Acetate	Acetate	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
13	Alanine	Ala	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
14	Arginine	Arg	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
15	Betaine	Betaine	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
16	Carnitine	Carnitine	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
17	Choline	Choline	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
18	Fumarate	Fumarate	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
19	Glucose	Glc	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
20	Glutamate	Glutamate	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
21	Glutamine	Gln	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
22	Glutathione	GSH	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
23	Homarine	Homarine	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
24	Hypotaurine	Hypotaurine	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
25	Isoleucine	Ile	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
26	Lactate	Lactate	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
27	Leucine	Leu	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
28	Maleate	Maleate	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
29	Maltose	Maltose	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
30	Methionine	Met	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
31	O-Acetylcholine	O-Acetylcholine	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
32	O-Phosphocholine	O-Phosphocholine	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
33	Propionate	Propionate	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
34	Pyruvate	Pyruvate	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
35	Serine	Ser	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
36	Succinate	Succinate	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
37	Taurine	Tau	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
38	Trimethylamine N-oxide	TMAO	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
39	Valine	Val	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
40	sn-Glycero-3-phosphocholine	sn-Glycero-3-phosphocholine	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
41	beta-Alanine	b-ala	mmol/l	García, Tomás	NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
42	Heat-shock protein 70	Hsp70	arbitrary units	García, Tomás	Protein separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); followed by immunoblotting	An integrative control sample (a pooled sample from all individuals) was included on each gel and used as an internal reference. Hsp70 levels were expressed in arbitrary units relative to this control
43	Catalase, unit per protein mass	CAT/prot	U/mg	García, Tomás	Microplate reader, BioTek, Synergy H1

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

5496 data points

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